Forensic Science Institute of Lanzhou Public Security

Lanzhou, China

Forensic Science Institute of Lanzhou Public Security

Lanzhou, China

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Yang X.,Gansu Institute of Political Science and Law | Yang X.,Forensic Science Institute of Lanzhou Public Security | Sun H.-B.,Gansu Institute of Political Science and Law | Sun H.-B.,Forensic Science Institute of Lanzhou Public Security | And 5 more authors.
Acta Anatomica Sinica | Year: 2014

Objective: To study the genetic relationship of the Y chromosomal short tandem repeat gene loci in Lanzhou Han population and other 25 populations.Methods: The frequency of alleles of Y-STRloci was obtained from a sample of 500 unrelated individuals living in Lanzhou City, and other 25 populations in different areas collected from the published data were used to calculate the genetic similarity coefficient and genetic distance. Phylogenetic trees based on the genetic distance were established.Results Populations of Lanzhou, Beijing, Shanxi and Inner Mongolia were in an identical cluster. Compared with minorities, the genetic distance between Lanzhou Han population and Inner Mongolia Mongolian population was dramatically smaller from other subpopulations. The populations in Malays and Indians were far from the other groups.Conclusion The Y-STR gene frequency distribution in 26 populations has identified differentiation in race, clime and evolution, and it is basically identical with the classification of human races which is similar to or according with other molecular anthropology research conclusions.


Zhang Z.,Xi'an Jiaotong University | Sun H.-B.,Key Laboratory of Evidence of Science and Technology Research and Application | Sun H.-B.,Forensic Science Institute of Lanzhou Public Security | Luo J.-H.,Key Laboratory of Evidence of Science and Technology Research and Application | And 3 more authors.
Journal of Forensic Medicine | Year: 2014

In the criminal cases of driving under the influence (DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis. The evidence can be acquired when the skin cells are observed on the surface of the airbag in a traffic accident. However, the low quantity or quality of the evidence collected from a crime scene prevents further identification analysis in many cases. In the current study, we reported a case of identifying touch DNA extraction from the shed skin cells from the deployed airbag of a motor vehicle. We managed to collect DNA evidence from the shed skin cells in an airbag using a proper approach of collection and extraction. The 5.87 ng of extracted DNA was sufficient for genotyping and forensic identification, which helped to identify the driver of the car in collision with a pier in the street. In DUI cases and other traffic accidents, therefore, the amount of touch DNA extracted from the deployed airbag can be sufficient for DNA marker genotyping and further analysis.


Luo J.,Key Laboratory of Evidence of Science and Technology Research and Application | Luo J.,Forensic Science Institute of Lanzhou Public Security | Sun H.,Key Laboratory of Evidence of Science and Technology Research and Application | Sun H.,Forensic Science Institute of Lanzhou Public Security | And 5 more authors.
Chinese Journal of Forensic Medicine | Year: 2014

Objective: To compare and improve forensic DNA extraction method from bones and teeth samples. Methods: Samples 18 bones and 30 teeth were acquired from skeletonized remains and triturated by TissueLyser-II. DNA materials were extracted from the triturated samples with PreFiler Express BTA™ forensic DNA extraction kit by Automate Express forensic DNA extraction system (BTA method) and decalcification method. DNA was quantified and followed by genotyping. The BTA method optimal samples size and paralysis time were determined by two femurs and eight molars from the same individual. Results: DNA extraction from bones and teeth were completed in 2.5 hours using TissueLyser-II and the BTA method. The quality of extracted DNA concentration is high for 18 bones and 30 teeth in the test, the same sample at different methods there were no significant difference between the same weight (P < 0.05), but the BTA method has more advantages in operation process. The success rate of bones and teeth STR typing were 94.4% and 96.7% using the BTA method, respectively. The results showed the optimal samples size 100mg and paralysis time 2h. Conclusion: The BTA method is an efficient method to extract DNA from bones and teeth, which are simple, can significantly improve the success rate of bones and teeth DNA test and can be applied in forensic practice.

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