Institute of Forensic Science
Institute of Forensic Science
Qian H.,Institute of Forensic Science
American Journal of Forensic Medicine and Pathology | Year: 2017
ABSTRACT: This report presents a case of a 52-year-old man who had a traffic accident and died later. External examination could not determine the cause of death. Approximately 3 weeks later, an autopsy was performed. Postmortem computed tomography was performed before the autopsy. Postmortem imaging was interpreted to show hemorrhage beneath the tentorium, and the C5 to C6 (the fifth and sixth cervical vertebra) disc space was widened. During the autopsy, a cervical spinal cord injury was confirmed. However, a meningioma was found under the tentorium instead of a hemorrhage. There are a number of reasons that include postmortem changes that affected the virtopsy diagnosis, which still needs development. © 2017 by Lippincott Williams & Wilkins.
Sayin U.,University of Wisconsin - Madison |
Sayin U.,Institute of Forensic science |
Hutchinson E.,University of Wisconsin - Madison |
Hutchinson E.,U.S. National Institutes of Health |
And 2 more authors.
Neuroscience | Year: 2015
Neural activity promotes circuit formation in developing systems and during critical periods permanently modifies circuit organization and functional properties. These observations suggest that excessive neural activity, as occurs during seizures, might influence developing neural circuitry with long-term outcomes that depend on age at the time of seizures. We systematically examined long-term structural and functional consequences of seizures induced in rats by kainic acid, pentylenetetrazol, and hyperthermia across postnatal ages from birth through postnatal day 90 in adulthood (P90). Magnetic resonance imaging (MRI), diffusion tensor imaging (DTI), and electrophysiological methods at ≥P95 following seizures induced from P1 to P90 demonstrated consistent patterns of gross atrophy, microstructural abnormalities in the corpus callosum (CC) and hippocampus, and functional alterations in hippocampal circuitry at ≥P95 that were independent of the method of seizure induction and varied systematically as a function of age at the time of seizures. Three distinct epochs were observed in which seizures resulted in distinct long-term structural and functional outcomes at ≥P95. Seizures prior to P20 resulted in DTI abnormalities in CC and hippocampus in the absence of gross cerebral atrophy, and increased paired-pulse inhibition (PPI) in the dentate gyrus (DG) at ≥P95. Seizures after P30 induced a different pattern of DTI abnormalities in the fimbria and hippocampus accompanied by gross cerebral atrophy with increases in lateral ventricular volume, as well as increased PPI in the DG at ≥P95. In contrast, seizures between P20 and P30 did not result in cerebral atrophy or significant imaging abnormalities in the hippocampus or white matter, but irreversibly decreased PPI in the DG compared to normal adult controls. These age-specific long-term structural and functional outcomes identify P20-30 as a potential critical period in hippocampal development defined by distinctive long-term structural and functional properties in adult hippocampal circuitry, including loss of capacity for seizure-induced plasticity in adulthood that could influence epileptogenesis and other hippocampal-dependent behaviors and functional properties. © 2014 IBRO.
News Article | December 25, 2015
Jorge* is around 60 years old, works a white collar job, has gray hair, married children, and grown grandchildren. People who work with him would never imagine that he participates in religious rituals using a mind altering tea. And yet, thanks to ayahuasca, Jorge became a teetotaler. A big change for someone who, when he was younger, would buy several cases of whiskey at once. “I opened the boxes and started emptying the bottles in the kitchen sink. My wife was shocked,” he told me. He’s not the only alcoholic to renounce booze after an experience with the mystical drink. In 2010, after a decade of failed treatments, Robert Rhatigan took a trip to the Peruvian Amazon, where he participated in four rituals conducted by a shaman. During a speech at a TEDx event, he recounted how he “saw” the “several components from his mind floating in space, as if they were pieces of a puzzle” while under the effects of ayahuasca. The experience lasted two hours and included hymns sung by the shaman and severe purging sessions. By the end of the ceremony, he “saw” the pieces returning to his head. The one that corresponded to his alcohol addiction no longer fit in. There he knew that he was cured. “My transformation is something far from understood in Western medicine,” he says. There are some hospitals, universities, and research institutes in the West and around the world that are experimenting with powerful psychoactive substances, such as psilocybin, ibogaine and even LSD are being analyzed in hospitals and research institutes all around the world. “Regarding ayahuasca studies, Brazil is at the forefront of research,” said Luis Fernando Tófoli, professor of the medical psychology and psychiatry department of Unicamp and coordinator of the Laboratory of Interdisciplinary Studies of psychoactive drugs, in Portuguese. This year, a study conducted by Brazilian researchers was published in Nature. The piece examined the effects of the drink on two men and four women who showed symptoms of depression, ranging from moderate to severe. The participants consumed ayahuasca only once in doses that ranged between 120ml to 200ml prepared by a church of Santo Daime. They then had their mental health monitored through three questionnaires repeated eight times, the first one 40 minutes after intake and the last one three weeks later. The results showed that there were improvements shown by every participant, disregarding the levels of depression they displayed. According to one of the surveys, one day after the experiment, there had been a reduction of 62 percent in symptoms. One week later, the efficacy kept going up, getting up to 72 percent. According to another survey, depression symptoms such as sadness, difficulty concentrating, suicidal and negative thoughts, had been reduced by 82 percent. Side effects were not detected, although half of the subjects had vomited under influence of the tea. The results impressed the researchers. “We observed antidepressant effects the first hours after administering ayahuasca, and they remained significant for two to three weeks,” Flávia de Lima Osório and Rafael Guimarães dos Santos, two of the authors, said in an email in Portuguese. She’s a lecturer in the department of neurosciences and behavioral sciences of the Universidade de São Paulo (USP) Medical School in Ribeirão Preto and he is a postdoctoral researcher in the same department. “Besides, ayahuasca was tolerated quite well by the patients. The majority described the experience as positive, even if there was vomiting and nausea.” The fast results could be good news for those who need quick-acting treatments. “Antidepressants that are currently available take weeks to produce therapeutic effects, in addition to having significant side effects, such as sexual dysfunction and weight gain,” Flávia wrote. “Many patients don't get an effective therapeutic response. New pharmaceuticals, that act faster and more efficiently with less side effects, are necessary.” Tófoli agreed. “Antidepressants that were recently introduced to the market, do the same as the older ones. New substances able to act on other receptors, haven't been introduced yet. Psychopharmacology has not been able to get that type of response,” he said. The combination of being thirsty for good news with the thrill associated to mind altering performed in labs can help one understand the hype that followed the release of the research. After becoming news on Nature, the research was highlighted by some leading news channels around the world, from Huffington Post to Scientific American. The news coverage made the researchers wary. Flávia requested to be interviewed only by email “because there were already issues with previous reports.” She also reiterated that nothing is really demonstrated. “This is a pilot-study, with a few volunteers and without double-blind testing controlled by placebo. On studies with antidepressants in general, the placebo effect can be very significant. Therefore, we can’t affirm yet that ayahuasca has antidepressant capabilities, let alone that it can cure depression,” she wrote. A new wave of ayahuasca enthusiasts may appear in the very near future. In Natal, the researcher Dráulio de Barros Araújo, from the Brain Institute of UFRN, coordinates a study using placebos to compare the effects of the tea in a group setting of 80 people, half of them diagnosed with depression. On top of receiving medical supervision, the subjects will be submitted to high definition electroencephalography (EEG) exams. “A study with this kind of consolidated methodology aiming to evaluate the potential benefits of ayahuasca for depression patients has never been done before” said Tófoli, one of the researchers. “If we find positive results, we have all we need to cause a certain impact.” Another study, published this year in the journal Physiology and Behaviour, analyzed the effects of ayahuasca on lab rats that were addicted to alcohol. The study was authored by Alexandre Justo de Oliveira-Lima, who is a pharmacology professor at State University of Santa Cruz; Eduardo Marinho, of the same university; and Laís Berro, of Federal University of São Paulo; along with researchers from Brás Cubas University and from the Institute of Forensic Science of São Paulo. Research with animals is one step in the development of new drugs and, also, a resource for better understanding of organic alterations caused by the process of addition. The problematic of use of drugs, by both animals and by humans, is linked to the feedback loop between the ventral tegmental area (VTA) and nucleus accumbens, which theoretically make up our reward and pleasure system. Oliveira-Lima already had experience with analyzing how drugs like anti-psychotics fight alcohol addiction. Stories about the possible benefits of ayahuasca caught his attention. “We decided to do this research because there’s this ambivalence regarding what causes benefits, if it’s the religious experience or the tea acting directly,” Lima said in Portuguese. During the study, rats were injected with ethyl alcohol to induce changes in cerebral activity and behavior. Their motor skills were then evaluated. Because low doses of alcohol have a stimulant power, the subjects tend to move through greater distances when they are under the influence. There were two experimental phases. During the first phase, the animals were separated into groups. One group received doses of saline, which served as a control. Others received doses of ayahuasca with different concentration levels and, later, alcohol injections. “We are simulating a situation where a person goes to a ritual, takes the tea and then drinks alcohol,” Lima said. One group received just alcohol injections, always with the same dose. Ten days later, the researchers compared the frequency with which the animals moved. The results showed that those who received ayahuasca moved about 50 percent less than those who only had alcohol, suggesting that the tea desensitized the rats to alcohol. The tea appeared to prevent the changes in cerebral activity induced by alcohol, resulting in milder behavioral responses. In the second experiment, the animals were again split in several groups. One received saline injections only to serve as a control. The remainder of them were sensitized by alcohol, that is, they became dependent. Afterwards, ayahuasca injections were administered for eight consecutive days, in attempt to revert the sensitization. After seven days, new alcohol injections were given and movement frequencies between groups were compared. The graph showed that animals that were experiencing the effects of alcohol for the first time, as well as the ones submitted to the desensitization process with the tea earlier, displayed exactly the same behavior. In other words: the “dependency” was reverted. It’s as if an individual who was addicted to alcohol went to rehabilitation and got treated by being given ayahuasca for just a few months, Olivera-Lima said. “With sensitization reversal, that person would be less prone to feel like drinking again,” he said. “And even if they relapsed, at first the experience would be milder.” In theory, as we learn more about the neurochemistry of ayahuasca, we could engineer medications that wouldn’t require patients to spend hours vomiting while hallucinating in a sweat lodge (by the way, it’s worth to point out that the rats didn't vomit during the study). “Nowadays there are few medical options that are effective enough for the treatment of alcoholism. From studies like these, pharmaceutical companies can become dedicated in developing new products. I believe that ayahuasca will be a big source for the treatment of addiction in the next decades,” Lima said. The next step for the researchers will be to repeat the process of sensitization and desensitization and then remove the brains from the rats to analyze the changes. Unfortunately, a recent budget cut for Brazil’s National Program of Post-Graduation will likely impair these experiments. “Although scholarships have been preserved, those cuts affect up 75 percent of the budget that cover the necessary means for the research,” Lima said. “One equipment line that was going to be purchased, for example, was cancelled for the year. That will reflect negatively on Brazilian science for the next five years.” One of the reasons Brazil has some of the most active research in ayahuasca is because back in the 80s, legislation was passed to permit religious use of the tea. “We are grateful for the religious groups because they gained rights and conditions that allow us now to do research,” said Tófoli. He emphasizes that studies have shown ayahuasca is not for everyone. “There are people who can have psychotic breakdowns,” he said. The ayahuasca debate still is far from resolution. There are many external factors that may play into the effectiveness of a medication. Getting a prescription involves so much more than just consuming a drug. That experience includes many seemingly irrelevant, but potentially important, sub-experiences. The patient is thinking, either subconsciously or consciously, about whatever negative stigma or positive association they have with the drug. They’re having an experience with the psychiatrist, and another at the pharmacy. There may be effects of stress or emotion at any of these stages. The same is true of ayahuasca, but such emotional impacts may be magnified because the drug is so mind-altering. This can introduce new ethical questions as well as uncertainties about what’s actually causing the effects. Recall the experience Robert Rhatigan had, where he cured his addiction after “seeing it” outside of his own mind. With ayahuasca, there seem to be more things involved than just neurochemistry. “Many people decide to change things about their lives during the experience. There are those who decide to become vegetarians, for example,” said Tófoli. “How would we talk about whether the benefits are caused by the chemistry or what a person experiences during the mind altering process? We can't be sure.” But wait. Didn't the experiment with the rats demonstrate that it is essentially a physiological matter, independent of what happens in the conscious mind of who is experimenting ayahuasca? “And who says that the rats don't have a conscious mind?” Tófoli said. A very psychedelic response. But it’s a good reminder that we’re still far from understanding the fundamentals of ayahuasca therapy. *Jorge requested his real name to be withheld. This article was translated from Motherboard Brazil.
Sun Y.,Tsinghua University |
Liu K.,Tsinghua University |
Miao J.,Tsinghua University |
Wang Z.,Tsinghua University |
And 5 more authors.
Nano Letters | Year: 2010
Surface-enhanced Raman scattering (SERS) has attracted wide attention because it can enhance normally weak Raman signal by several orders of magnitude and facilitate the sensitive detection of molecules. Conventional SERS substrates are constructed by placing metal nanoparticles on a planar surface. Here we show that, if the planar surface was substituted by a unique nanoporous surface, the enhancement effect can be dramatically improved. The nanoporous surface can be easily fabricated in batches and at low costs by cross stacking superaligned carbon nanotube films. The as-prepared transparent and freestanding SERS substrate is capable of detecting ambient trinitrotoluene vapor, showing much higher Raman enhancement than ordinary planar substrates because of the extremely large surface area and the unique zero-dimensional at one-dimensional nanostructure. These results not only provide a new approach to ultrasensitive SERS substrates, but also are helpful for improving the fundamental understanding of SERS phenomena. © 2010 American Chemical Society.
Li K.,CAS Shanghai Institute of Applied Physics |
Qin W.,CAS Shanghai Institute of Applied Physics |
Li F.,CAS Shanghai Institute of Applied Physics |
Zhao X.,Institute of Forensic Science |
And 5 more authors.
Angewandte Chemie - International Edition | Year: 2013
Search for traces: Aptamer-bound Au nanoparticles (Au NPs) were used to provide high-resolution dark-field microscopy images of latent fingerprints (LFPs) with level 2 and level 3 details. Furthermore, the cocaine-induced aggregation of Au NPs results in a true green-to-red color change of the scattered light, providing a quasi-quantative method to identify cocaine loadings in LFPs. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Li F.,CAS Shanghai Institute of Applied Physics |
Pei H.,CAS Shanghai Institute of Applied Physics |
Wang L.,CAS Shanghai Institute of Applied Physics |
Lu J.,Wenzhou Medical College |
And 4 more authors.
Advanced Functional Materials | Year: 2013
A variety of nanomaterials have shown extraordinarily high quenching ability toward a broad range of fluorophores. Recently, there has been intense interest in developing new tools for fluorescent DNA analysis in solution or inside the cell based on this property, and by exploiting interactions between these nanoscale "superquenchers" and DNA molecules in the single-stranded (ss-) or double-stranded (ds-) forms. Here, a comparative study on the nanoqueching effects is performed by using a series of nanomaterials with different dimensions, i.e., gold nanoparticles (AuNPs, 0D), carbon nanotubes (CNTs, 1D), and graphene oxide (GO, 2D). The quenching efficiency, kinetics, differentiation ability, and influencing factors such as concentration and ionic strength are studied. Interestingly, GO exhibits superior quenching abilities to the other two materials in both the quenching efficiency and kinetics. As a result, a GO-based fluorescent sensor, designed in a simple mix-and-detect format, can detect concentrations of DNA as low as 0.2 nM, which is better than either CNTs or AuNPs by an order of magnitude. This sensor can also differentiate single-base mismatches much better than either CNTs- or AuNPs- based sensors. This study paves the way to better choice of nanomaterials for bioanalysis and elaborate design of biosensors for both in vitro diagnosis and in vivo bioimaging. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Qian H.,Institute of Forensic Science
American Journal of Forensic Medicine and Pathology | Year: 2016
ABSTRACT: This report presents a case of a 40-year-old woman who was found dead in her house. The examination of the body revealed no external injuries. The whole body was scanned by multi–detector-row computed tomography (CT) before autopsy, revealing massive hemorrhage in the right frontal extending into the ventricular system. At autopsy, the brain parenchyma was removed. Then CT angiography was carried on the isolated brain. Computed tomography angiography suggested a mass of irregular, tortuous vessels in areas of hemorrhage in the right frontal lobe of the brain. Finally, histological examination confirmed the result of CT angiography due to an arteriovenous malformation. Hence, postmortem CT angiography played an important role in diagnosis of the cerebral arteriovenous malformation that was responsible for a massive hemorrhage in the skull. © 2016 by Lippincott Williams & Wilkins.
Wang T.,Institute of Forensic science
American Journal of Forensic Medicine and Pathology | Year: 2016
ABSTRACT: Electrocution cases reported in the literature were mainly caused by low-voltage alternating current, with few cases associated with high-voltage direct current. In this article, we report the case of a 48-year-old woman who unexpectedly and suddenly died in the wild because of electric shock by a high-voltage direct current while hunting. The postmortem inspection found a suspected current mark on her right upper thigh, and histological examination of the suspected current mark revealed a slight elongation of the epidermal cell nuclei and separation of the corneous layer. Metal elements analysis by microbeam x-ray fluorescence spectrometry demonstrated a peak concentration of iron in the suspected current-injured skin surface, which was in line with the metal wire at the scene. In addition, the deceased had coronary atherosclerotic heart disease, which can increase the bodyʼs risk to the current damage. Our results indicated the usefulness of microbeam x-ray fluorescence spectrometry for the forensic diagnosis of electrocution. © 2016 by Lippincott Williams & Wilkins.
Li C.T.,Institute of Forensic science
Genetics and molecular research : GMR | Year: 2011
Allele frequencies of 30 insertion/deletion polymorphism (InDel) markers previously selected and validated for forensic purposes were assessed in 419 unrelated individuals originating from five different populations of P.R. China, including Chinese Han, Chinese Hui, Uighur, Mongolians, and Tibetans. Hardy-Weinberg equilibrium tests and linkage disequilibrium analysis were performed; the allele frequency distributions of the 30 InDel markers met the conditions for genetic equilibrium in all five populations and the InDel markers on the same chromosome did not generate any linkage blocking. Analysis of molecular variance indicated that genetic variation among the five populations represents only 4% of the total genetic diversity. We determined the cumulative power of discrimination for each population: 0.99999999999841 in Chinese Han, 0.99999999999690 in Chinese Hui, 0.99999999999709 in Uighur, 0.99999999999772 in Mongolians, and 0.99999999999854 in Tibetans.
Zhang S.H.,Institute of Forensic science
Genetics and molecular research : GMR | Year: 2012
Individual identification of urinary samples is necessary when sample switching or handling are suspected during a judicial process. To improve the rate of successful genotyping of urinary samples, we examined the stability of DNA in urinary samples stored for up to 30 days. Urinary samples from 20 healthy individuals (10 males and 10 females) were stored at -80°C with different concentrations of EDTA (0, 10 and 40 mM). Urinary DNA was extracted at days 0, 3, 9, and 30 after collection. The Quantifiler Human DNA Quantification Kit was used for measuring DNA concentration. Twenty STR loci were co-amplified using amelogenin-specific PCR with the Goldeneye 20A Kit. Significant differences in DNA concentration were observed between samples from females and males. In the case of female urinary DNA preservation with 10 and 40 mM EDTA the mean detection rate reached 0.95 after up to 30 days; for the male urinary samples, the mean detection rate of urinary DNA preserved with 40 mM EDTA was significantly higher than with 10 mM. We concluded that 40 mM EDTA is the best concentration for preservation of the DNA in urinary samples.