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Chavez-Briones M.L.,Autonomous University of Nuevo Leon | Chavez-Briones M.L.,Forensic Genetics Laboratory | Hernandez-Cortes R.,Forensic Genetics Laboratory | Jaramillo-Rangel G.,Autonomous University of Nuevo Leon | Ortega-Martinez M.,Autonomous University of Nuevo Leon
Genetics and Molecular Research | Year: 2015

Bite mark evidence has been repeatedly found in criminal cases. Physical comparison of a bite mark to the teeth of available suspects may not always be possible. Experimental studies have shown that the analysis of DNA present in the saliva recovered from bite marks might help in the identification of individuals. However, the application of this approach to an actual criminal case has been reported only once before in forensic literature. Therefore, there is very limited scientific and technical information available on this subject. The current study focuses on a woman found dead in her home; the autopsy ruled the death to be a result of manual strangulation. A bite mark was found on each breast. The single swab technique was used to collect evidence from these bite marks, and an organic extraction method was employed for DNA isolation. Short tandem repeat (STR) sequence typing was performed using a commercially available kit, and the result was compared to the STR profile of a suspect. A full singlesource STR profile was obtained from both bite marks, which matched the STR profile of the suspect. To the best of our knowledge, this is the second report on the analysis of DNA isolated from bite marks on the victim used to identify the crime perpetrator. Our results indicated that, contrary to most theoretical indications, a single swab technique for evidence collection and an organic method for DNA isolation could be very useful in solving this class of criminal cases. © FUNPEC-RP. Source

Vullo C.M.,Forensic Genetics Laboratory | Romero M.,Forensic Genetics Laboratory | Catelli L.,Forensic Genetics Laboratory | Sakic M.,International Commission for Missing Persons ICMP | And 13 more authors.
Forensic Science International: Genetics | Year: 2016

The GHEP-ISFG Working Group has recognized the importance of assisting DNA laboratories to gain expertise in handling DVI or missing persons identification (MPI) projects which involve the need for large-scale genetic profile comparisons. Eleven laboratories participated in a DNA matching exercise to identify victims from a hypothetical conflict with 193 missing persons. The post mortem database was comprised of 87 skeletal remain profiles from a secondary mass grave displaying a minimal number of 58 individuals with evidence of commingling. The reference database was represented by 286 family reference profiles with diverse pedigrees. The goal of the exercise was to correctly discover re-associations and family matches. The results of direct matching for commingled remains re-associations were correct and fully concordant among all laboratories. However, the kinship analysis for missing persons identifications showed variable results among the participants. There was a group of laboratories with correct, concordant results but nearly half of the others showed discrepant results exhibiting likelihood ratio differences of several degrees of magnitude in some cases. Three main errors were detected: (a) some laboratories did not use the complete reference family genetic data to report the match with the remains, (b) the identity and/or non-identity hypotheses were sometimes wrongly expressed in the likelihood ratio calculations, and (c) many laboratories did not properly evaluate the prior odds for the event. The results suggest that large-scale profile comparisons for DVI or MPI is a challenge for forensic genetics laboratories and the statistical treatment of DNA matching and the Bayesian framework should be better standardized among laboratories. © 2015 Elsevier Ireland Ltd. All rights reserved. Source

Montesino M.,University Institute of Research in Forensic science | Tagliabracci A.,Marche Polytechnic University | Zimmermann B.,Innsbruck Medical University | Gusmao L.,University of Porto | And 29 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

In this GHEP-ISFG exercise, participating labs were invited to evaluate a forensic case in which the mtDNA haplotype from a hair shaft in the victim's hand matched the suspect's haplotype. 31 forensic labs participated in the exercise. Although all except one used the EMPOP database to estimate the haplotype frequencies different final likelihood ratios (LRs) were reported. The main factors affecting these differences were: the origin of the reference population, the approaches to correct sampling errors, the LR formula, the source of EMPOP data (forensic/literature), the type of search (pattern or literal and "disregard Indels" option) and the selected edition range to perform the queries. This demonstrates that further efforts are needed in order to standardize the statistical evaluation of the mtDNA evidence. © 2011 Elsevier Ireland Ltd. Source

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A third tour has been added to the Laboratory Design Conference agenda. The conference will be held April 25-27 in Houston, Texas. . Sign up to tour the Harris County Forensics facility (Existing DNA Laboratory and New Building): In January 2013, the Harris County Institute of Forensic Sciences opened its 15,000 sq. ft., state-of-the-art Forensics Genetics Laboratory within a renovated train bay of Houston’s historic Nabisco cookie plant, today known as the Texas Medical Center’s John P. McGovern Campus.  The location of the laboratory in the TMC campus allows for close collaboration with other renowned medical and research science faculties. The Institute’s Forensic Genetics Laboratory received a Certificate of Recognition for Excellence in Construction from the Texas Building Branch of the Associated General Contractors. The project consisted of 17,000 sq. ft. designed for Serology Labs, Extraction Labs, Evidence Receiving/Storage, and administrative space for the laboratory. Vaughn Construction is serving as the construction manager-at-risk, with Johnston L.L.C. as the architect. The new, 140,000 sq. ft. Main Campus of the Harris County Institute of Forensic Sciences is currently being constructed on a 3.2-acre site directly across from its existing location. The comprehensive planning process for the building, which will house both the Medical Examiner and Crime Laboratory services for Harris County, began in 2007 and incorporated experts from business, medical, laboratory and scientific fields. The resulting design culminates in an integrated use of space flowing seamlessly between clinical, laboratory, administrative, public and teaching/training areas. Consideration was also given to the exterior design of the facility to ensure it complements the aesthetics of neighboring institutions located on the Texas Medical Center Campus. The new Forensic Center is expected to be completed in 2017. Vaughn Construction is serving as the construction manager-at-risk, with Page/ as the architect. The other facilities offered as part of the Laboratory Design Conference's tour package are Brockman Hall for Physics at Rice University, and Texas Medical Center. Click here for more information about these facilities . Tours of exemplary lab facilities, including those to which attendees would not otherwise have access, are an integral part of the overall   experience. Breakfast and round-trip transportation from the hotel to these sites will be provided.

Ottaviani E.,Forensic Genetics Laboratory | Vernarecci S.,Forensic Genetics Laboratory | Asili P.,Forensic Genetics Laboratory | Agostino A.,Thermo Fisher Scientific | Montagna P.,Forensic Genetics Laboratory
International Journal of Legal Medicine | Year: 2015

A total of 150 samples of unrelated males from North of Italy were analyzed with the prototype Yfiler® Plus kit (Thermo Fisher Scientific, Oyster Point, CA). This kit is a short tandem repeat (STR) assay based on six-dye chemistry that amplifies 25 Y-STR loci. Sixteen loci are in common with Yfiler® kit and nine are new (DYS576, DYS627, DYS460, DYS518, DYS570, DYS449, DYS481, DYF387S1a/b, DYS533). In this population study, the improvement of adding additional Y-STR markers increased the discriminatory capacity from 0.787 with the minimum haplotype (MHT) loci to 1 with the prototype Yfiler® Plus kit. © 2014, Springer-Verlag Berlin Heidelberg. Source

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