Entity

Time filter

Source Type

Prague, Czech Republic

Ehler E.,Charles University | Vanek D.,Forensic DNA Service | Vanek D.,Charles University | Stenzl V.,Institute of Criminalistics | And 2 more authors.
Croatian Medical Journal | Year: 2011

Aim: To evaluate Y-chromosomal diversity of the Moravian Valachs of the Czech Republic and compare them with a Czech population sample and other samples from Central and South-Eastern Europe, and to evaluate the effects of genetic isolation and sampling. Methods: The first sample set of the Valachs consisted of male donors from the Valach region in northeastern Czech Republic border-area. The second sample set of the Valachs consisted of 79 men who originated from lineages defined by surname. No close relatives were sampled. The third sample set consisted of 273 unrelated men from the whole of the Czech Republic and was used for comparison, as well as published data for other. The total number of samples was 3244. Yshort tandem repeat (STR) markers were typed by standard methods using PowerPlex® Y System (Promega) and Yfiler® Amplification Kit (Applied Biosystems) kits. Y-chromosomal haplogroups were estimated from the haplotype information. Haplotype diversity and other intra- and inter-population statistics were computed. Results: The Moravian Valachs showed a lower genetic variability of Y-STR markers than other Central European populations, resembling more to the isolated Balkan populations (Aromuns, Csango, Bulgarian, and Macedonian Roma) than the surrounding populations (Czechs, Slovaks, Poles, Saxons). We illustrated the effect of sampling on Valach paternal lineages, which includes reduction of discrimination capacity and variability inside Y-chromosomal haplogroups. Valach modal haplotype belongs to R1a haplogroup and it was not detected in the Czech population. Conclusion: The Moravian Valachs display strong substructure and isolation in their Y chromosomal markers. They represent a unique Central European population model for population genetics. Source


Votrubova-Dubska J.,Forensic DNA Service | Vanek D.,Forensic DNA Service | Vanek D.,Charles University | Vanek D.,Institute of Legal Medicine | And 4 more authors.
Forensic Science International | Year: 2016

During a rescue excavation in October 2011, archaeologists discovered a mass grave with 10 individuals. The skeletons should belong to victims of the battle of Reichenberg between the Austrian and Prussian armies on April 21, 1757. Several bones of the skeletons were covered with a blue colored encrustation. Initial DNA analysis failed due to strong inhibition. Chemical analysis of the bluish encrustation indicated the presence of the iron phosphate mineral vivianite (Fe3(PO4)2·(H2O)8). This technical note describes a novel procedure for the removal of this inhibitory substance. © 2016 Elsevier Ireland Ltd. Source


Vanek D.,Forensic DNA Service | Vanek D.,Charles University | Saskova L.,Forensic DNA Service | Silerova M.,Forensic DNA Service | And 4 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

142 unrelated males from the population sample of the Czech Republic were genotyped using the miniSTR pentaplexes I and II in 8 Y-chromosomal STRs not included in Y-filer kit (Applied Biosystems, USA). A total of 142 haplotypes were obtained of which 127 were unique. The most common haplotype were found in 4 samples. We observed the haplotype diversity of 0.998 and the discrimination capacity of 0.894. © 2011 Elsevier Ireland Ltd. Source


Vanek D.,Forensic DNA Service | Vanek D.,Charles University | Silerova M.,Forensic DNA Service | Urbanova V.,Forensic DNA Service | And 3 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

120 unrelated individuals from the population sample of the Czech Republic were genotyped using the AmpFℓSTR® NGM™ PCR amplification kit (Applied Biosystems). The population study was conducted to evaluate the usefulness of the five new STR loci (D10S1248, D22S1045, D2S441, D1S1656, and D12S391) included in the new European Standard Set and to establish the allele frequencies. © 2011 Elsevier Ireland Ltd. Source


Vanek D.,Forensic DNA Service | Vanek D.,Charles University | Silerova M.,Forensic DNA Service | Urbanova V.,Forensic DNA Service | And 3 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

The aim of this study was to develop an extraction protocol for bone samples based on ZR Genomic DNA Tissue MicroPrep kit and perform a quantitative comparison with the existing silica extraction protocol based on Qiagen columns and evaluate the effect of demineralization on the quantity of extracted DNA. © 2011 Elsevier Ireland Ltd. Source

Discover hidden collaborations