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Choi S.-Y.,Hannam University | Park G.-S.,Forensic Center | Lee S.Y.,Korea Research Institute of Bioscience and Biotechnology | Kim J.Y.,Korea Research Institute of Bioscience and Biotechnology | Kim Y.K.,Korea Research Institute of Bioscience and Biotechnology
Archives of Pharmacal Research | Year: 2011

In the course of searching for cholesteryl ester transfer protein (CETP) inhibitors from natural sources, a new type of CETP inhibitor, [10]-dehydrogingerdione (1), was isolated from the extract of rhizomes of Zingiber officinale Roscoe. By NMR spectroscopic analysis of its 1HNMR, 13C-NMR, and 1H-1H COSY, HMBC, HMQC and NOESY, more precise structure, compared with its originally proposed structures, of [10]-dehydrogingerdione has been elucidated. This active compound inhibited human plasma CETP with IC50 values of 35 μM. © 2011 The Pharmaceutical Society of Korea and Springer Netherlands.


News Article | March 16, 2016
Site: www.labdesignnews.com

A third tour has been added to the Laboratory Design Conference agenda. The conference will be held April 25-27 in Houston, Texas. . Sign up to tour the Harris County Forensics facility (Existing DNA Laboratory and New Building): In January 2013, the Harris County Institute of Forensic Sciences opened its 15,000 sq. ft., state-of-the-art Forensics Genetics Laboratory within a renovated train bay of Houston’s historic Nabisco cookie plant, today known as the Texas Medical Center’s John P. McGovern Campus.  The location of the laboratory in the TMC campus allows for close collaboration with other renowned medical and research science faculties. The Institute’s Forensic Genetics Laboratory received a Certificate of Recognition for Excellence in Construction from the Texas Building Branch of the Associated General Contractors. The project consisted of 17,000 sq. ft. designed for Serology Labs, Extraction Labs, Evidence Receiving/Storage, and administrative space for the laboratory. Vaughn Construction is serving as the construction manager-at-risk, with Johnston L.L.C. as the architect. The new, 140,000 sq. ft. Main Campus of the Harris County Institute of Forensic Sciences is currently being constructed on a 3.2-acre site directly across from its existing location. The comprehensive planning process for the building, which will house both the Medical Examiner and Crime Laboratory services for Harris County, began in 2007 and incorporated experts from business, medical, laboratory and scientific fields. The resulting design culminates in an integrated use of space flowing seamlessly between clinical, laboratory, administrative, public and teaching/training areas. Consideration was also given to the exterior design of the facility to ensure it complements the aesthetics of neighboring institutions located on the Texas Medical Center Campus. The new Forensic Center is expected to be completed in 2017. Vaughn Construction is serving as the construction manager-at-risk, with Page/ as the architect. The other facilities offered as part of the Laboratory Design Conference's tour package are Brockman Hall for Physics at Rice University, and Texas Medical Center. Click here for more information about these facilities . Tours of exemplary lab facilities, including those to which attendees would not otherwise have access, are an integral part of the overall   experience. Breakfast and round-trip transportation from the hotel to these sites will be provided.


Kim M.H.,Yonsei University | Kim M.H.,Forensic Center | Kim Y.,Yonsei University | Kim J.W.,Yonsei University | And 5 more authors.
Plant and Cell Physiology | Year: 2013

Brassinosteroids (BRs) activate the BRI1 and BAK1/SERK3 membrane receptor complex, which leads to a wide range of changes in gene expression, plant growth and development. As an initial step to elucidate additional roles of BAK1, we cloned a BAK1-binding protein, BAK1-Associating Receptor-Like Kinase 1 (BARK1), and characterized its gene expression and root phenotypes. BARK1 is a putative membrane LRR-RLK (leucine-rich repeat receptor-like kinase) protein that specifically binds to BAK1 and its homologs. Careful examination of BARK1 expression using transgenic plants expressing a green fluorescent protein (GFP) reporter under the control of the native BARK1 promoter (BARK1p::GFP) revealed that this gene is ubiquitously expressed in most plant tissues, and shows especially strong expression in the xylem vasculature of primary and lateral roots as well as in mature pollen. Interestingly, the expression of the BARK1 gene was increased in the BR biosynthetic loss-of-function mutant, det2, and a loss-of-function mutant of BR signaling, bak1-3. In contrast, this gene was down-regulated in the bzr1-1D plant, which is a BR signal gain-of-function mutant. BARK1-overexpressing transgenic plants clearly enhanced primary root growth in a dose-dependent manner, and their roots were hypersensitive to BR-induced root growth inhibition. In addition, both the number and density of lateral roots were dramatically increased in the BARK1 transgenic plants in a dose-dependent manner. Together with observations that ARF (AUXIN RESPONSE FACTOR) genes are up-regulated in the BARK1 overexpressor, we suggest that the BARK1 overexpressor phenotype with more lateral roots is partly due to the increased expression of ARF genes in this genetic background. In conclusion, BAK1-interacting BARK1 protein may be involved in BR-mediated plant growth and development such as in lateral roots via auxin regulation. © 2013 The Author.


Tak Y.K.,Seoul National University | Kim W.Y.,Seoul National University | Kim M.J.,Seoul National University | Han E.,Seoul National University | And 5 more authors.
Analytica Chimica Acta | Year: 2012

Forensic DNA samples can degrade easily due to exposure to light and moisture at the crime scene. In addition, the amount of DNA acquired at a criminal site is inherently limited. This limited amount of human DNA has to be quantified accurately after the process of DNA extraction. The accurately quantified extracted genomic DNA is then used as a DNA template in polymerase chain reaction (PCR) amplification for short tandem repeat (STR) human identification. Accordingly, highly sensitive and human-specific quantification of forensic DNA samples is an essential issue in forensic study. In this work, a quantum dot (Qdot)-labeled Alu sequence was developed as a probe to simultaneously satisfy both the high sensitivity and human genome selectivity for quantification of forensic DNA samples. This probe provided PCR-free determination of human genomic DNA and had a 2.5-femtogram detection limit due to the strong emission and photostability of the Qdot. The Qdot-labeled Alu sequence has been used successfully to assess 18 different forensic DNA samples for STR human identification. © 2012 Elsevier B.V.


PubMed | University of the Philippines at Diliman, Forensic Center and University of the Philippines at Manila
Type: | Journal: Forensic science international. Genetics | Year: 2015

The capability of DNA laboratories to perform genotyping procedures from post-mortem remains, including those that had undergone putrefaction, continues to be a challenge in the Philippines, a country characterized by very humid and warm conditions all year round. These environmental conditions accelerate the decomposition of human remains that were recovered after a disaster and those that were left abandoned after a crime. When considerable tissue decomposition of human remains has taken place, there is no other option but to extract DNA from bone and/or teeth samples. Routinely, femur shafts are obtained from recovered bodies for human identification because the calcium matrix protects the DNA contained in the osteocytes. In the Philippines, there is difficulty in collecting femur samples after natural disasters or even human-made disasters, because these events are usually characterized by a large number of fatalities. Identification of casualties is further delayed by limitation in human and material resources. Hence, it is imperative to test other types of biological samples that are easier to collect, transport, process and store. We analyzed DNA that were obtained from body fluid, bone marrow, muscle tissue, clavicle, femur, metatarsal, patella, rib and vertebral samples from five recently deceased untreated male cadavers and seven male human remains that were embalmed, buried for 1 month and then exhumed. The bodies had undergone different environmental conditions and were in various stages of putrefaction. A DNA extraction method utilizing a detergent-washing step followed by an organic procedure was used. The utility of bone marrow and vitreous fluid including bone marrow and vitreous fluid that was transferred on FTA() cards and subjected to autosomal STR and Y-STR DNA typing were also evaluated. DNA yield was measured and the presence or absence of PCR inhibitors in DNA extracts was assessed using Plexor()HY. All samples were amplified using PowerPlex()21 and PowerPlexY()23 systems and analyzed using the AB3500 Genetic Analyzer and the GeneMapper() ID-X v.1.2 software. PCR inhibitors were consistently detected in bone marrow, muscle tissue, rib and vertebra samples. Amplifiable DNA was obtained in a majority of the samples analyzed. DNA recovery from 0.1g biological material was adequate for successful genotyping of most of the non-bone and bone samples. Complete DNA profiles were generated from bone marrow, femur, metatarsal and patella with 0.1 ng DNA template. Using 0.5 ng DNA template resulted in increased allele recovery and improved intra- and inter-locus peak balance.


Lee H.,Chung - Ang University | Lee H.,Forensic Center | Bae S.,Chung - Ang University | Yoon Y.,Chung - Ang University
Journal of Nutritional Biochemistry | Year: 2013

(-)Epigallocatechin gallate (EGCG) is the most abundant catechin in green tea and reportedly has anti-obesity and anti-adipogenic effects. In this study, we determined that the up-regulation of the WNT/β-catenin pathway is the anti-adipogenic mechanisms of EGCG in 3T3-L1 cells. EGCG treatment down-regulates the expression of major genes involved in the adipogenesis pathway including peroxisome proliferator-activated receptor (PPAR)γ, CCAAT/enhancer binding protein (C/EBP)α, fatty acid binding protein (FABP)4 and fatty acid synthase (FASN), while up-regulating the nuclear level of β-catenin. Knockdown of β-catenin using small interfering (si) RNA attenuated the inhibitory effects of EGCG on intracellular lipid accumulation. β-catenin siRNA transfection also recovered terminal adipocyte markers such as FABP4, FASN, lipoprotein lipase and adiponectin, which were down-regulated by EGCG. The DNA binding activities as well as the expression levels of PPARγ and C/EBPα, which were down-regulated by EGCG, were significantly restored by β-catenin siRNA transfection. In addition, we found that EGCG efficiently up-regulates the WNT/β-catenin pathway. Among the members of the WNT/β-catenin pathway, the expressions of low density lipoprotein receptor-related protein (LRP)5, LRP6, disheveled (DVL)2 and DVL3 were significantly up-regulated, while AXIN expression was down-regulated by EGCG, and the phosphorylation of glycogen synthase kinase 3β was increased. These results suggest that EGCG activates the WNT/β-catenin pathway, resulting in the up-regulation of β-catenin, which down-regulates the major genes of the adipogenesis pathway. Taken together, our findings clearly show that the anti-adipogenic effects of EGCG are, at least partially, dependent on the WNT/β-catenin pathway. © 2013 Elsevier Inc.


Lee H.,Chung - Ang University | Lee H.,Forensic Center | Bae S.,Chung - Ang University | Yoon Y.,Chung - Ang University
International Journal of Molecular Medicine | Year: 2012

1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D, was found to have anti-adipogenic activity, however, its mechanism of action has not been fully elucidated. In this study, 3T3-L1 preadipocytes were differentiated in the presence and absence of 1,25(OH)2D3, and the expression of the genes and proteins of the wingless-type MMTV integration site (WNT)/β-catenin pathway were analyzed. While the expression of the members of the WNT/β-catenin pathway were significantly downregulated during the adipogenesis of untreated 3T3-L1 cells, 1,25(OH)2D3 treatment was found to maintain the WNT/β-catenin pathway. Among the members of the WNT/β-catenin pathway, the levels of WNT10B and disheveled (DVL)2 as well as the phosphorylation of glycogen synthase kinase (GSK)3β were maintained by 1,25(OH)2D3 treatment. The levels of nuclear β-catenin, which were downregulated during adipogenesis, were also maintained by 1,25(OH)2D3 treatment. The results of this study suggested that the anti-adipogenic effect of 1,25(OH)2D3 was mediated by the maintenance of the WNT/β-catenin pathway, which was normally downregulated during adipogenesis.


Choung C.M.,Forensic Center | Lee D.S.,Forensic Center | Park K.W.,Forensic Center | Han M.S.,Forensic Center
Forensic Science International: Genetics Supplement Series | Year: 2011

Current forensic DNA typing is conducted in approximately 8-9. h. Whole steps included DNA extraction step, Quantification, PCR amplification step, electrophoresis process through capillary separation with fluorescence detection, data analysis and DNA profile interpretation. Among them, we have tested rapid PCR method of AmpF. lSTR Identifler PCR to reduce running time. We have altered several PCR conditions of ordinary AmpF. lSTR Identifler PCR method and used 9947A control DNA to cut the time for the PCR reaction. In the results of this study, the critical step of the PCR reaction was the annealing step and also we reduced PCR running times by 1/3 to 2/3 (approximately 60-90. min) with complete concordance of STR allele calls using standard reference material 9947A. © 2011 Elsevier Ireland Ltd.


Ma Y.,Forensic Center | Ma Y.,China Pharmaceutical University | Liu J.,China Pharmaceutical University | Hou J.,China Pharmaceutical University | And 6 more authors.
PLoS ONE | Year: 2014

Diabetes mellitus type 1 (DM1) is an autoimmune disease that gradually destroys insulin-producing beta-cells. We have previously reported that mucosal administration of fusion protein of HSP65 with tandem repeats of P277 (HSP65-6P277) can reduce the onset of DM1 in non-obese diabetic (NOD) mice. To deliver large amounts of the fusion protein and to enhance long-term immune tolerance effects, in the present study, we investigated the efficacy of using orally administrated L. lactis expressing HSP65-6P277 to reduce the incidence of DM1 in NOD mice. L. lactis strain NZ9000 was engineered to express HSP65-6P277 either constitutively or by nisin induction. After immunization via gavage with the recombinant L. lactis strains to groups of 4-week old female NOD mice for 36 weeks, we observed that oral administration of recombinant L. Lactis resulted in the prevention of hyperglycemia, improved glucose tolerance and reduced insulitis. Immunologic analysis showed that treatment with recombinant L. lactis induced HSP65- and P277- specific T cell immuno-tolerance, as well as antigen-specific proliferation of splenocytes. The results revealed that the DM1-preventing function was in part caused by a reduction in the pro-inflammatory cytokine IFN-γ and an increase in the anti-inflammatory cytokine IL-10. Orally administered recombinant L. lactis delivering HSP65-6P277 may be an effective therapeutic approach in preventing DM1. © 2014 Ma et al.


Seong K.M.,Forensic Center | Seong K.M.,Kongju National University | Park J.H.,Forensic Center | Hyun Y.S.,Kongju National University | And 5 more authors.
Forensic Science International: Genetics | Year: 2014

We assessed the applicability of 30 insertion-deletion polymorphisms (INDELs) in forensic use and the level of genetic diversity in South Korea (n = 373) using the Investigator DIPplex® kit (Qiagen). Allele frequencies, heterozygocities, and forensic efficacy parameters were determined. No deviation from Hardy-Weinberg equilibrium was observed for any of the INDEL markers. A high level of discrimination power was observed (combined power of discrimination: 0.99999999995). The combined match probability value was 2.84 × 10-11 and the mean typical paternity indices were 0.878. Furthermore, we found one microvariant allele at HLD93 (rs2307570) that has not been reported. We expect that these 30 loci of INDEL markers will be useful for forensic identification and paternity testing in the South Korean population. © 2013 Elsevier Ireland Ltd.

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