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Hollosi L.,Thermo Fisher Scientific | Mittendorf K.,Thermo Fisher Scientific | Senyuva H.Z.,FoodLife International Ltd.
Chromatographia | Year: 2012

This paper describes an on-line sample preparation method for the simultaneous determination of 48 pesticides in grapes, baby food and wheat flour matrices. Target pesticides were selected to represent a wide variety of chemical structures and three typical matrices were selected. Turbulent flow chromatography was applied for on-line sample cleanup directly coupled to LC-MS/MS. The aim of the method was to reduce total analysis time, eliminate manual laboratory work, provide clean extracts and achieve reproducible results. Single laboratory method validation was conducted establishing limits of detection between 0.8 and 6.0 ng g-1 for baby food, and 0.8-10.3 ng g-1 for other matrices. Within-day precision values varied between 4 and 18 %, while between-day precisions were in the range 5-22 %. Method recovery ranged from 67 to 124 %, and method accuracy was demonstrated by analysis of external quality control samples. The method was also tested on 24 different survey samples from both bio and organic origin. The method was shown to be convenient, fast and fit for purpose in meeting regulatory requirements for pesticide residue monitoring. © Springer-Verlag Berlin Heidelberg 2012. Source

Senyuva H.Z.,FoodLife International Ltd. | Gokmen V.,Hacettepe University | Sarikaya E.A.,Thermo Fisher Scientific
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2015

A literature search from 2007 to 2014 was conducted to identify publications where principally LC-Orbitrap™-high-resolution mass spectrometry (HRMS) has been employed in food analysis. Of a total of 212 relevant references, only 22 papers were from 2007–10, but in subsequent years there has been a steady growth in publications with 38–55 relevant papers being published each year from 2011 to 2014. In the food safety area, over 50% of the published papers were equally divided between pesticides, veterinary drug residues and natural toxins (including mycotoxins) focused primarily on multi-analyte target analysis. LC-Orbitrap-HRMS was also found to be increasingly important for the analysis of bioactive substances, principally phenolic compounds in foods. A number of studies reported for the first time the identification of new fungal metabolites, predominantly various conjugated forms of known mycotoxins. Novel process contaminants were also identified by LC-Orbitrap-HRMS, as were various substances used for food adulteration and bioactive substances in herbal products and dietary supplements. Untargeted analysis is seen as a major future trend where HRMS plays a significant role. Retrospective analysis of scanned high-resolution mass spectra in conjunction with relevant databases can provide new insights. Metabolomics is also being increasingly used where foods are being profiled through fingerprinting using HRMS. All evidence points towards future growth in the number of applications of HRMS in food safety and quality, as the power of this technique gains wider recognition. © 2015 Taylor & Francis. Source

Bousova K.,Thermo Fisher Scientific | Senyuva H.,FoodLife International Ltd. | Mittendorf K.,Thermo Fisher Scientific
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2012

A fast and reliable multiresidue method is reported for the identification and quantification of 36 different compounds from seven different classes of antibiotics (aminoglycosides, sulfonamides, macrolides, quinolones, tetracyclines, lincosamides and trimethoprim) in milk. Automated online sample cleanup was applied using turbulent flow chromatography (Transcend TLX system), directly coupled to a mass spectrometer (MS/MS) for sensitive and specific detection. The method involved a simple extraction/protein precipitation using acetonitrile, followed by centrifugation and filtration. After this preliminary step, the extract was injected into the TLX-ESI-MS/MS using optimised turbulent flow and liquid chromatography (LC) conditions. Single-laboratory validation of the method was carried out according to the Directive 2002/657/EC, clearly demonstrating the suitability of this method for quantitative determination of this wide range of antibiotics in milk. A small survey, which covered milk samples of different origin and varied fat content, demonstrated the robustness of this method and its suitability for enforcement purposes. © 2012 Copyright Taylor and Francis Group, LLC. Source

Ates E.,Thermo Fisher Scientific | Godula M.,Thermo Fisher Scientific | Stroka J.,Institute for Reference Materials and Measurements | Senyuva H.,FoodLife International Ltd.
Food Chemistry | Year: 2014

A wide range of plant and fungal metabolites can occur in cereals and feed but only a limited number of target compounds are sought. This screening method is using a database of over 600 metabolites to establish contamination profiles in food and feed. Extracts were injected directly into an automated turbulent flow sample clean-up system, coupled to a liquid-chromatography-high-resolution- mass-spectrometer (Orbitrap). Compound identification criteria for database searching were defined and the approach was validated by spiking plant and fungal metabolites into cereals and feed. A small survey of market samples (15) and quality control materials (9) of maize, wheat and feed was conducted using this method. Besides regulated and known secondary metabolites, fumiquinazoline F, fusarochromanone and dihydrofusarubin were identified for the first time in samples of maize and oats. This method enables clean-up of crude extracts within 18 min and screening and confirmation of a wide range of different compound classes. © 2013 Elsevier Ltd. All rights reserved. Source

Bucchini L.,Hylobates Consulting Srl | Guzzon A.,Hylobates Consulting Srl | Poms R.,MoniQA Association | Senyuva H.,FoodLife International Ltd.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2016

As part of a European Union-funded project (FP7) developing ‘Integrated approaches to food allergen and allergy management’, a database was constructed based on publicly available information on food allergen recalls in Europe, North America, Hong Kong, Australia and New Zealand. Over 2000 entries were made into the database. The database covers a 4-year period from 2011 to 2014 and each entry is categorised into food type (two different classifications), identified allergen and cause where indicated by the authorities. Across different authorities, by far the biggest incidence of undeclared allergens occurred in the food categories of prepared dishes and snacks (range = 12–53%), and cereals and bakery products (range = 14–25% of all recalls and/or alerts). The biggest incidence of undeclared allergens, according to the information from most authorities, occurred for milk and milk products (16–31% of all products with recall or alert), followed by cereals containing gluten (9–19%), soy (5–45%), and egg and egg products (5–17%). Although 42–90% of the products with recalls/alerts were explained as being ‘Not indicated on the label’, this is a generic explanation of cause and does not provide much insight into the causes of the recall/alerts. However, 0–17% of products with recalls/alerts could be coded as caused by the unintended presence of an allergen as the probable result of cross-contact in production. Construction of the database of allergen recalls has provided some important lessons and recommendations to the authorities are made in this paper in terms of the harmonisation of the reporting of allergen recalls into a more standardised format. © 2016 Informa UK Limited, trading as Taylor & Francis Group Source

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