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Ankara, Turkey

Ulca P.,Mega Center No | Ozturk Y.,Mega Center No | Senyuva H.Z.,FoodLife International
Food Additives and Contaminants: Part B Surveillance | Year: 2011

Surveys were carried out between 2007 and 2010 to determine the total levels of sulfites in 1245 samples of wines, dried apricots, dried vegetables, nuts, juices and purees, frozen foods and cereals containing dried fruit supplied by food inspectors and by food producers for testing or for export certification. Sulfite analysis of wine was carried out using the Ripper method with an LOQ of 5mg l -1 and for dried and other foods the Monier-Williams distillation procedure was employed with an LOQ of 10 mgkg -1. In the survey all wines contained measurable sulfites, but with the exception of one sample of white wine they were otherwise below Turkish Food Codex limits of 160mg kg -1 for red wine, 210 mgkg -1 to white wine and 235 mgkg -1 for sparkling wine. None of the cereal products, frozen foods, juices or purees contained sulfites above 10 mgkg -1. However, all dried apricot samples contained significant levels of sulfite with around 40% having levels exceeding the Turkish limit of 2000mg kg -1. Significant levels of sulfite were found in other samples of dried fruit with even a fruit and nut bar containing 1395 mg kg -1 of sulfite, suggesting the dried fruit ingredients contained levels above regulatory limits. © 2011 Taylor & Francis. Source


Ates E.,Thermo Fisher Scientific | Mittendorf K.,Thermo Fisher Scientific | Senyuva H.,FoodLife International
Journal of AOAC International | Year: 2013

An automated sample preparation technique involving cleanup and analytical separation in a single operation using an online coupled TurboFlow (RP-LC system) is reported. This method eliminates time-consuming sample preparation steps that can be potential sources for crosscontamination in the analysis of plasticizers. Using TurboFlow chromatography, liquid samples were injected directly into the automated system without previous extraction or cleanup. Special cleanup columns enabled specific binding of target compounds; higher MW compounds, i.e., fats and proteins, and other matrix interferences with different chemical properties were removed to waste, prior to LC/MS/MS. Systematic stepwise method development using this new technology in the food safety area is described. Selection of optimum columns and mobile phases for loading onto the cleanup column followed by transfer onto the analytical column and MS detection are critical method parameters. The method was optimized for the assay of 10 phthalates (dimethyl, diethyl, dipropyl, butyl benzyl, diisobutyl, dicyclohexyl, dihexyl, diethylhexyl, diisononyl, and diisododecyl) and one adipate (diethylhexyl) in beverages and milk. © 2014 Publishing Technology. Source


Ulca P.,Mega Center No | Balta H.,Mega Center No | Cagin T.,Mega Center No | Senyuva H.Z.,FoodLife International
Meat Science | Year: 2013

The method performance characteristics of commercially available PCR kits for animal species identification were established. Comminuted meat products containing different levels of pork were prepared from authentic beef, chicken, and turkey. These meat products were analysed in the raw state and after cooking for 20. min at 200. °C. For both raw and cooked meats, the PCR kit could correctly identify the animal species and could reliably detect the addition of pork at a level below 0.1%. A survey of 42 Turkish processed meat products such as soudjouk, salami, sausage, meatball, cured spiced beef and doner kebap was conducted. Thirty-six samples were negative for the presence of pork (<0.1%) and four were found to be correctly labelled as containing pork. However, one sausage sample was labelled as containing 5% beef, but beef DNA was not detected and a meatball sample labelled as 100% beef was found to contain chicken. Another turkey meatball sample was predominantly chicken. © 2013 Elsevier Ltd. Source


Demirhan Y.,Mega Center No | Ulca P.,Mega Center No | Senyuva H.Z.,FoodLife International
Meat Science | Year: 2012

A commercially available real-time PCR, based on a multi-copy target cytochrome b (cyt b) using porcine specific primers, has been validated for the Halal/Kosher authentication of gelatine. Extraction and purification of DNA from gelatine were successfully achieved using the SureFood® PREP Animal system, and real-time PCR was carried out using SureFood® Animal ID Pork Sens kit. The minimum level of adulteration that could be detected was 1.0% w/w for marshmallows and gum drops. A small survey was undertaken of processed food products such as gum drops, marshmallows and Turkish delight, believed to contain gelatine. Of fourteen food products from Germany, two samples were found to contain porcine gelatine, whereas of twenty-nine samples from Turkey twenty-eight were negative. However, one product from Turkey contained porcine DNA and thus was not Halal, and neither was the use of porcine gelatine indicated on the product label. © 2011 Elsevier Ltd. Source


Bousova K.,Thermo Fisher Scientific | Senyuva H.,FoodLife International | Mittendorf K.,Thermo Fisher Scientific
Journal of Chromatography A | Year: 2013

A multi-class method for identification and quantification of 36 antibiotics from seven different chemical classes (aminoglycosides, macrolides, lincosamides, sulfonamides, tetracyclines, quinolones and trimethoprim) has been developed by using liquid chromatography-mass spectrometry. The method was optimised for detection of antibiotics in chicken meat. Sample preparation including extraction with a mixture of acetonitrile:2% trichloroacetic acid (45:55, v/v), centrifugation and filtration was followed by on-line clean-up using turbulent flow chromatography. Using this automated on-line technique enabled a larger number of samples to be analysed per day than with a traditional clean-up technique (e.g. solid phase extraction). The optimised method was validated according to the European Commission Directive 2002/657/EC. In-house validation was performed by fortifying the blank matrix at three levels 0.5, 1.0 and 1.5 MRL (maximum residue limit), or respectively, at concentrations as low as possible for substances without an MRL. Precision in terms of repeatability standard deviation ranged from 3 to 28% and recovery values were between 80 and 120% in most cases. All calculated validation parameters including CCα and CCβ were in the compliance with the legislative requirements. © 2012 Elsevier B.V. Source

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