Russo P.,University of Foggia |
Russo P.,Promis Biotech srl |
Iturria I.,Food Research Unit |
Mohedano M.L.,CSIC - Biological Research Center |
And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2015
A critical feature of probiotic microorganisms is their ability to colonize the intestine of the host. Although the microbial potential to adhere to the human gut lumen has been investigated in in vitro models, there is still much to discover about their in vivo behaviour. Zebrafish is a vertebrate model that is being widely used to investigate various biological processes shared with humans. In this work, we report on the use of the zebrafish model to investigate the in vivo colonization ability of previously characterized probiotic lactic acid bacteria. Lactobacillus plantarum Lp90, L. plantarum B2 and Lactobacillus fermentum PBCC11.5 were fluorescently tagged by transfer of the pRCR12 plasmid, which encodes the mCherry protein and which was constructed in this work. The recombinant bacteria were used to infect germ-free zebrafish larvae. After removal of bacteria, the colonization ability of the strains was monitored until 3 days post-infection by using a fluorescence stereomicroscope. The results indicated differential adhesion capabilities among the strains. Interestingly, a displacement of bacteria from the medium to the posterior intestinal tract was observed as a function of time that suggested a transient colonization by probiotics. Based on fluorescence observation, L. plantarum strains exhibited a more robust adhesion capability. In conclusion, the use of pRCR12 plasmid for labelling Lactobacillus strains provides a powerful and very efficient tool to monitor the in vivo colonization in zebrafish larvae and to investigate the adhesion ability of probiotic microorganisms. © 2015, Springer-Verlag Berlin Heidelberg.
Perera M.P.M.S.H.,University of Peradeniya |
Sivakanesan R.,University of Peradeniya |
Abeysekara D.T.D.J.,University of Peradeniya |
Sarananda K.H.,Food Research Unit
Pakistan Journal of Nutrition | Year: 2014
Traditional Sri Lankan breakfast foods such as pittu, rotti, wandu, hopper and thosai made with 75% rice flour (Bg 352) and 25% soy flour (Pb 1) mixture were tested for their Glycaemic Index (Gl) and Glycaemic Load (GL). Pittu, rotti, wandu, hopper and thosai showed low Gl values of 35.5, 36.0, 43.0, 45.2 and 47.3, respectively. The GL of pittu, rotti, wandu, hopper and thosai were 5.4, 7.5, 5.4, 6.6 and 6.7, respectively and could be considered as low. The Gl of foods diminished when incorporated in composite meals. A vegetable mixed curry was prepared mixing Solanum melongena (eggplant), Lycopersicon esculentum (tomato), Cucurbita maxima (pumpkins) and Ipomoea aquatica (Kankun/water spinach) in order to study meal Gl and meal GL. The amount of fat, protein and available carbohydrate in the vegetable mixed curry were 9.2, 4.3 and 6.6 g/100 g dry weight, respectively. The Gl of pittu meal, rotti meal, wandu meal, hopper meal and thosai meal were 30.2, 31.2, 36.5, 38.3 and 43.8, respectively. Pittu meal, rotti meal, wandu meal, hopper meal and thosai meal showed GL values of 3.9, 5.0, 4.1, 4.9 and 6.0, respectively. Addition of vegetable mixed curry showed considerable reduction of Gl and GL of all test meals. © Asian Network for Scientific Information, 2014.
Abeywickrama K.,University of Kelaniya |
Wijerathna C.,University of Kelaniya |
Rajapaksha N.,University of Kelaniya |
Kannangara S.,University of Kelaniya |
Sarananda K.,Food Research Unit
Acta Horticulturae | Year: 2013
Anthracnose (Colletotrichum gloeosporioides), stem-end rot (Phomopsis caricaepapayae and Lasiodiplodia theobromae), and Phomopsis rot of papaya are major diseases resulting in relatively high post-harvest losses (up to 45%) in Sri Lanka. Current consumer preference is for tropical fruits free of agrochemicals. The objectives of the present investigation were to identify the in vitro efficacy of basil oil (Ocimum basillicum) and alum (sodium aluminium sulphate) on fungal pathogens of papaya fruit and to develop a relatively safe, in vivo integrated disease control strategy. Alum (1% (w/v)) and basil oil (0.12-0.16% (v/v)) in a liquid bioassay, indicated fungistatic and fungicidal efficacy on Colletotrichum gloeosporioides, Lasiodiplodia theobromae and Phomopsis caricae-papayae isolated from 'Red Lady' and 'Rathna' cultivars of papaya. In vivo investigation of fruits of both cultivars harvested from orchards in Sri Lanka at 25% maturity level indicated that papaya washed in 1% (w/v) alum and subsequently sprayed with an emulsion solution of 0.16% (v/v) basil oil and enclosed in styrofoam sleeves could be stored for a period of 14 days at 12-14°C. The visual quality rating (VQR) was high (11-13), and shriveling and "green islands" were low on fruits with zero disease severity where results were statistically similar to a Bavistin 500 mg/L (systemic fungicide) treatment. Physicochemical properties assessed were unaffected by the treatments and were comparable to the control (washed only in water). The organoleptic properties were slightly low in fruits subjected to cold temperature compared to fruits subjected to ambient temperature. No residues of basil oil could be detected on fruits by gas chromatography at the end of the experimental period. This integrated treatment strategy of papaya could be adopted for sea shipment and temperature-regulated supermarket storage of papaya fruits where storage life could be lengthened by up to 14 days. © 2013 ISHS.
Kang M.-C.,Jeju National University |
Kim S.Y.,Jeju National University |
Kim Y.T.,Jeju National University |
Kim E.-A.,Jeju National University |
And 8 more authors.
Carbohydrate Polymers | Year: 2014
The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications. © 2013 Elsevier Ltd. All rights reserved.
Wijesinghe W.A.J.P.,Jeju National University |
Wijesinghe W.A.J.P.,Food Research Unit |
Kim E.-A.,Jeju National University |
Kang M.-C.,Jeju National University |
And 4 more authors.
Environmental Toxicology and Pharmacology | Year: 2014
5β-Hydroxypalisadin B, a halogenated secondary metabolite isolated from red seaweed Laurencia snackeyi was evaluated for its anti-inflammatory activity in lipopolysaccharide (LPS)-induced zebrafish embryo. Preliminary studies suggested the effective concentrations of the compound as 0.25, 0.5, 1. μg/mL for further in vivo experiments. 5β-Hydroxypalisadin B, exhibited profound protective effect in the zebrafish embryo as confirmed by survival rate, heart beat rate, and yolk sac edema size. The compound acts as an effective agent against reactive oxygen species (ROS) formation induced by LPS and tail cut. Moreover, 5β-hydroxypalisadin B effectively inhibited the LPS-induced nitric oxide (NO) production in zebrafish embryo. All the tested protective effects of 5β-hydroxypalisadin B were comparable to the well-known anti-inflammatory agent dexamethasone. According to the results obtained, 5β-hydroxypalisadin B isolated from red seaweed L. snackeyi could be considered as an effective anti-inflammatory agent which might be further developed as a functional ingredient. © 2013 Elsevier B.V.
Wijesinghe J.A.A.C.,University of Sri Jayewardenepura |
Wicramasinghe I.,University of Sri Jayewardenepura |
Saranandha K.H.,Food Research Unit
Pakistan Journal of Nutrition | Year: 2016
Detailed studies have been carried out on Kithul (Caryota urens) native and modified flour as two pre-gelatinized treatments (PGI and PGII), acid modified (AC) and dextrinized modification (DX) flour treatments to compare gelatinization properties and amylose content. Among all treatments, PG-II (modified with excess water at 75°C heat treatment) showed significant changes with higher gelatinization temperatures (To = 74.02°C and Tp = 80.51°C) and lower Conclusion temperature (Tc = 90.04°C), gelatinization enthalpy (5.78 J/g) and amylose content (25.36). This study provides evidence that PGII is the most suitable modification treatment for native Kithul flour for introducing new flour source with an applicable form to meet specific needs with a view to effort expand application within the food industry. © Asian Network for Scientific Information, 2016.