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Seoul, South Korea

Park S.Y.,Sahmyook University | Lee D.K.,Sahmyook University | An H.M.,Sahmyook University | Kim J.R.,Sahmyook University | And 7 more authors.
Biotechnology and Biotechnological Equipment

In the present study Lactobacillus plantarum CBT1209, Pediococcus pentosaceus CBT SL4, and Bifidobacterium longum SPM1205 showed curd formation in soy milk for 8 hours of incubation time in a single-culture experiment. Among these strains, B. longum SPM1205 as a new isolated strain, inhibited the activity of fecal harmful enzymes of rat. Therefore, we investigated the possibility of using a mixed culture containing B. longum SPM1205 for obtaining a curd-type soy-based yogurt with respect to curd formation, proximate composition and sensory attributes. Then we examined the changes in pH, lactic acid, and lactic acid bacteria during cold storage. B. longum SPM1205 was capable of producing moderate sour taste and good overall acceptability in curd-type plain soy-based yogurt for 8 hours of fermentation. Most sensory qualities were not significantly different in the strawberry soy-based yogurt compared to those of commercial milk-based yogurt. The pH of the strawberry soy-based yogurt decreased significantly (P < 0.05) during the days of storage (day 0, 5.18; day 1, 4.80), but the decrease slowed down after 3 days of storage. The lactic acid content and lactic acid bacteria population of plain and strawberry soy-based yogurt were not significantly changed over 10 days of storage. The products contained over 8 log of viable lactic acid bacteria. The results provide feasibility that a curd-type soy-based yogurt can be manufactured using B. longum SPM1205. However, more experiments are needed to improve the sensory qualities such as beany or astringent flavor and color. Source

Park S.Y.,Sahmyook University | Lee D.K.,Sahmyook University | An H.M.,Sahmyook University | Cha M.G.,Sahmyook University | And 6 more authors.
Iranian Journal of Biotechnology

A total of twenty-two strict anaerobic and Gram-positive Bifidobacteria, identified as B. adolescentis, B. pseudocatenulatum, or B. longum, were isolated from healthy adult Koreans. We here investigated the cell morphology, antimicrobial resistance patterns to novel antibiotics and genotypic differentiation of Bifidobacteria assessing repetitive DNA element PCR (rep-PCR) fingerprinting using the BOXA1R primer at the species level. All Bifidobacterium spp., except B. adolescentis SPM1005 and B. longum SPM1205, formed round and convex colonies. All B. adolescentis, B. pseudocatenulatum, and B. longum were opaque white glossy in colony color, and short, long, and irregular rods in morphological shape. In addition, all B. adolescentis, B. pseudocatenulatum, and B. longum formed a variety of shapes ranging from rods to Vshaped, Y-shaped, clubbed rods, or irregular. All Bifidobacterium spp., except B. adolescentis SPM0214, were sensitive to daptomycin (DAP), linezolid (LIN), and tigecycline (TIG). B. adolescentis SPM0214 was resistant to DAP. Genomic fingerprinting patterns of B. adolescentis, B. pseudocatenulatum, and B. longum were diverse and different from those of the KCTC strain. The band size of B. adolescentis, B. pseududocatenulatum, and B. longum varied from 3.0 kb to 300 bp, 2.0 kb to 200 bp, and 2.0 kb to 500 bp, respectively. In conclusion, twenty-two strains of B. adolescentis, B. pseudocatenulatum, and B. longum isolated from healthy adult Koreans were very diverse in both phenotype and genotype. Moreover, this diversity of phenotype and genotype may support that health promoting effects of individual strain of Bifidobacterium spp. human isolates could be different and specific even within same species. Source

Dela Pena I.J.I.,Sahmyook University | Hong E.,Food R and nter | Kim H.J.,Sahmyook University | De La Pena J.B.,Sahmyook University | And 3 more authors.
American Journal of Chinese Medicine

The Artemisia group of plants has long been used as a traditional remedy for various conditions. The present study assessed the sleep-promoting (sedative-hypnotic) effects of Artemisia capillaris Thunberg (A. capillaris), and elucidated a possible mechanism behind its effect. ICR mice were given A. capillaris extract (oral) at different dosages (50, 100, 200, 300, or 400 mg/kg), distilled water (oral; control), or diazepam (intraperitoneal; reference drug). One hour after administration, locomotion (open-field test) and motor coordination (rota-rod test) were assessed. The extract's effect on pentobarbital-induced sleep was also evaluated. Additionally, electroencephalographic (EEG) recordings were measured in rats. To evaluate a possible mechanism behind its effects, changes in chloride (Cl-) ion influx were measured in human neuroblastoma cells. As compared to the control group, mice treated with A. capillaris demonstrated significantly decreased locomotor activity and impaired motor balance and coordination. The extract also shortened the onset and lengthened the duration of sleep induced by pentobarbital sodium. These effects were comparable to that induced by diazepam. Furthermore, A. capillaris-treated rats showed increased delta and decreased alpha EEG waves; an electroencephalographic pattern indicative of relaxation or sedation. In neuroblastoma cells, the extract dose-dependently increased Cl- ion influx, which was blocked by co-administration of bicuculline, a GABAA receptor competitive antagonist, suggesting that its effects are mediated through the GABAA receptor-Cl- ion channel complex. Altogether, the results of the present study demonstrate that A. capillaris possesses potent sedative-hypnotic effects, which are probably mediated through potentiation of the GABAA receptor-Cl- ion channel complex. © 2015 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine. Source

dela Pena I.J.I.,Sahmyook University | de la Pena J.B.,Sahmyook University | Yoon S.Y.,Sahmyook University | Kim H.J.,Sahmyook University | And 5 more authors.
Natural Product Sciences

Laurus nobilis (L. nobilis) is traditionally used as an herbal medicine to treat various diseases. Ethanol (EtOH) consumption entails physiological, mental and psychomotor alterations. The aim of the present study was to assess the effects of L. nobilis in attenuating the EtOH-induced psychomotor alterations. L. nobilis was administered to SD rats, 30 minutes before EtOH administration (4 g/kg), at doses of 25, 50 and 100 mg/kg. Evaluations of psychomotor activity in the open-field, accelerating rota-rod, wire, and swimming ability were done at 1, 2, 4 and 8 hours after EtOH administration. In addition, blood ethanol and acetaldehyde levels were also measured. Pre-treatment of L. nobilis significantly improved EtOH-induced psychomotor alterations and decreased blood ethanol and acetaldehyde levels. These findings suggest that L. nobilis might be an effective substance to attenuate the harmful effects of EtOH, particularly psychomotor alterations, and can potentially be considered as a functional food. Source

Dela Pena I.J.I.,Sahmyook University | Hong E.,Food R and nter | De La Pena J.B.,Sahmyook University | Kim H.J.,Sahmyook University | And 5 more authors.
Journal of Medicinal Food

Milk has long been known and used to promote sleep. The sleep-promoting effect of milk has been attributed to its psychological associations (i.e., the memory of a mother giving milk at bedtime) and its rich store of sleep-promoting constituents (e.g., tryptophan). Studies have shown that milk harvested at night (Night milk) contains exceptionally high amounts of tryptophan and melatonin. In the present study, we evaluated the psychopharmacological properties of Night milk, particularly its probable sleep-promoting/enhancing, and anxiolytic effects. Night milk was orally administered to ICR mice at various concentrations (100, 200, or 300mg/kg). An hour after administration, assessment of its sedative (open-field and rotarod tests) and sedative sleep-potentiating effects (pentobarbital-induced sleeping test) was conducted. For comparison, the effects of Day milk (daytime milking) were also assessed. In addition, the effects of Night milk on anxiety behavior (elevated plus maze [EPM] test) and electroencephalographic (EEG) waves were evaluated. Night milk-treated animals exhibited decreased spontaneous locomotion (open-field test) and impaired motor balance and coordination (rotarod test). Furthermore, Night milk shortened the sleep onset and prolonged the sleep duration induced by pentobarbital sodium. These effects were comparable to that of diazepam. In addition, Night milk significantly increased the percentage of time spent and entries into the open arms of the EPM, indicating that it also has anxiolytic effects. No significant changes in EEG waves were observed. Altogether, these findings suggest that Night milk is a promising natural aid for sleep- and anxiety-related disturbances. © Copyright 2015, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2015. Source

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