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Kim J.-Y.,National Institute of Chemical Safety | Chae Y.-S.,South Korea National institute of Food and Drug Safety Evaluation | Moon J.-A.,Bureau of Food Nutrition and Dietary Safety | Baek S.-H.,Korea University
Food Science and Technology Research | Year: 2014

Establishment of an analytical method on zilpaterol (ZPT), the beta-agonists, and a growth promoting agent for livestock was undertaken. Analytical method for ZPT in the beef by LC-ESI-MS/MS was adopted. Before sample extraction, a beef sample was hydrolyzed by beta-glucuronidase/arylsulfatase and 0.2 M ammonium acetate buffer solution. Afterwards, the mixture was extracted with ethylacetate and then defatted by n-hexane/methanol partitioning. Subsequently, the separated organic layer was evaporated and the dissolved solution was purified by using a Molecular Imprinted Polymer solid phase extraction. The quantitation was carried out using LC-ESIMS/ MS with C18, a non-polarity column under the gradient condition with 10 mM ammonium acetate (pH 4.3): acetonitrile (95 : 5 (v/v), a mobile phase A) and acetonitrile (a mobile phase B). The standard calibration curve presented linearity of correlation coeficient (r2) > 0.999, analyzed from 0.2 to 10 ng/mL. The limit of quantitation in a beef showed 0.2 μg/kg. The average recoveries of intra- and inter-day experiment ranged from 100.9 to 108.5% and 103.3 to 104.5%, respectively. The repeatability and reproducibility for intra- and inter-day measurement expressed as a relative standard deviation was less than 10.0%, respectively. Therefore, the established analytical method for ZPT residue in beef was applicable to the official analytical method with the acceptable level of sensitivity, repeatability and reproducibility. Copyright © 2014, Japanese Society for Food Science and Technology

Kim J.-Y.,National Institute of Chemical Safety | Song H.-Y.,Wonkwang University | Moon J.-A.,Bureau of Food Nutrition and Dietary Safety | Shin M.-H.,Planning and Budget Office | Baek S.-H.,Korea University
Korean Journal of Food Science and Technology | Year: 2014

This study was carried out to examine the quality properties of honey in Korean commercial markets. The moisture content, stable carbon isotope ratio, invert sugar, cane sugar, and hydroxy-methylfurfural (HMF) contents of honey were measured according to the Korea Food Code and AOAC's (Association of Official Analytical Chemists) official methods. The stable carbon isotope ratio ranged from -25.18 to -12.60, which clearly differed between honey of C3 origin (flower) and C4 origin (artificial). Results of quality measurements revealed a moisture content of 18.12 to 19.70%, fructose content of 36.10 to 43.94%, glucose content of 22.61 to 31.91%, sucrose content of 1.56 to 4.75%, invert sugar content of 64.89 to 72.79%, and HMF content of 4.10 to 78.66 mg/kg. These values demonstrate that the quality of the tested honey meets the standard criteria of the Korean Food Code and Codex. However, it is necessary to reconsider the appropriate criteria for imported honey because it is circulated in the market through a long distribution process. © The Korean Society of Food Science and Technology.

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