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Irfan M.,University of Punjab | Nadeem M.,Food & Biotechnology Research Center | Syed Q.,Food & Biotechnology Research Center | Qazi J.I.,University of Punjab
Energy Sources, Part A: Recovery, Utilization and Environmental Effects | Year: 2016

In this study response surface methodology (RSM) was applied to study the effect of H2SO4 concentration, temperature, and time on the production of reducing sugars, total sugars, and total phenolic compounds from pine needles. Three variables with three levels showed that maximum release of total phenolic compounds (31.20 ± 0.002 mM) was observed at 1% H2SO4 concentration, 130°C temperature for 75 min of residence time. Under these conditions, the predicted value of total phenolic compounds was 31.27 mM, which indicated that the model is valid, having negligible variation in observed and predicted values. These results suggested that this substrate could be potentially used as substrate for bioethanol production. © 2016 Taylor & Francis Group, LLC.

Siddique A.,Food & Biotechnology Research Center | Gul A.,Food & Biotechnology Research Center | Irfan M.,Food & Biotechnology Research Center | Irfan M.,University of Sargodha | And 2 more authors.
Biofuels | Year: 2016

Biofuel production is popular with biotechnologists due to the continuously increasing need for energy. One major issue is to get maximum fuel production for minimum cost. In our studies we focused on both factors and developed a method for bioethanol production by investing in low cost chemicals. We compared three chemical pretreatments (NaOH, Na2SO3 and H2O2) at 121°C temperature and 15 psi pressure for delignification to evaluate the efficacy of chemicals in pretreatment. NaOH and Na2SO3 showed comparable percentage delignification (81.3 and 80.17% respectively) while H2O2 showed 67.4% delignification. Endogenously produced cellulolytic enzyme with 30 IU/mL CMCase and 22 IU/mL FPase units was used for saccharification of exposed cellulose. An indigenously developed strain of Saccharomyces cerevisiae SC36 (developed in a peptone free high gravity media) was used to ferment saccharified sugars into ethanol at 37°C fermentation temperature. We were able to convert 27% of the total bagasse (as per dry mass basis) into ethanol with NaOH pretreatment followed by 26% bagasse (as per dry mass basis) into ethanol with Na2SO3 pretreatment and 1% ethanol was obtained from H2O2 treated bagasse. © 2016 Informa UK Limited, trading as Taylor & Francis Group

Nadeem M.,Food & Biotechnology Research Center | Aftab M.U.,Food & Biotechnology Research Center | Irfan M.,Food & Biotechnology Research Center | Mushtaq M.,University of Punjab | And 2 more authors.
Frontiers in Life Science | Year: 2015

The present study was designed to produce ethanol from sugarcane bagasse treated with 2.5% NaOH through a simultaneous saccharification and fermentation process using Saccharomyces cerevisiae G1. Various process parameters such as incubation temperature, incubation period, initial pH and nitrogen sources were studied to achieve the maximum yield of ethanol. The results showed that the optimum ethanol yield (5.90%) was achieved at an incubation temperature of 30°C, initial pH 5.5, inoculum size 3% (v/v), after an incubation period of 96 h. Among various nitrogen sources, ammonium sulphate was found to be the most suitable source for ethanol production. These findings indicate that the optimization of process parameters is necessary to make the fermentation process economical, and the medium designed in the present study could be exploited on a commercial scale after suitable processing. © 2015 Taylor & Francis.

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