Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita

Rome, Italy

Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita

Rome, Italy
SEARCH FILTERS
Time filter
Source Type

Maranghi F.,Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita | Lorenzetti S.,Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita | Tassinari R.,Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita | Moracci G.,Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita | And 16 more authors.
Reproductive Toxicology | Year: 2010

The plasticizer di-(2-ethylhexyl)phthalate (DEHP) affects reproductive development, glycogen and lipid metabolism. Whereas liver is a main DEHP target in adult rodents, the potential impact on metabolic programming is unknown. Effects of in utero DEHP exposure on liver development were investigated upon treatment of pregnant CD-1 mice on gestational days (GD)11-19. F1 mice were examined at post-natal days 21 (weaning) and 35 (start of puberty): parameters included liver histopathological, immunocytochemical and α-fetoprotein (AFP) gene expression analyses. In utero DEHP exposure altered post-natal liver development in weanling mice causing significant, dose-related (i) increased hepatosteatosis, (ii) decreased glycogen storage, (iii) increased β-catenin intracytoplasmic localization (females only). At puberty, significantly decreased glycogen storage was still present in males. A treatment-induced phenotype was identified with lack of glycogen accumulation and intracytoplasmic localization of β-catenin which was associated with increased AFP gene expression. Our findings suggested that DEHP alters post-natal liver development delaying the programming of glycogen metabolism. © 2010 Elsevier Inc.

Loading Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita collaborators
Loading Veterinary And Food Toxicology Unit Instituto Superiore Of Sanita collaborators