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Lei L.,Food and Nutritional science Programme | Li Y.M.,Food and Nutritional science Programme | Wang X.,Food and Nutritional science Programme | Liu Y.,Food and Nutritional science Programme | And 5 more authors.
European Journal of Lipid Science and Technology | Year: 2015

The present study investigated the underlying mechanism by which citrus polymethoxylated flavones (PMF) reduced plasma triacylglycerols (TG) in hamsters. Four groups of male hamsters were given a non-cholesterol diet (NCD), a high cholesterol diet (HCD), a high cholesterol diet with supplementation of 0.5% PMF (L-PMF), and a high cholesterol diet with supplementation of 1.0% PMF (H-PMF), respectively, for 8 weeks. Results showed PMF could lower plasma TG by 32-46%, but it had no effect on plasma cholesterol. This was accompanied by down-regulation of mRNA of liver sterol regulatory element binding protein-1c (SREBP1c), fatty acid synthase (FAS), and peroxisome proliferator activated receptor alpha (PPARα) while up-regulation of liver lipoprotein lipase (LPL). In addition, dietary PMF increased cholesterol by 5-15% and total lipids by 34-43% in the liver. PMF supplementation also reduced the body weight gain and the relative weights of white adipose tissue pads by 23-39%, possibly by decreasing the gene expression of FAS and LPL in the epididymal fat pad. It was concluded that plasma TG-lowering activity of dietary PMF was mediated by modulating genes involved in lipid metabolism in hamsters. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Jiao R.,Food and Nutritional science Programme | Guan L.,Food and Nutritional science Programme | Yang N.,Food and Nutritional science Programme | Peng C.,Food and Nutritional science Programme | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

Dietary cholesterol elevates plasma total cholesterol (TC) level. However, no study to date has examined how cholesterol intake frequency interacts with the gene of sterol transporters, receptors, and enzymes involved in cholesterol metabolism. Thirty-three hamsters were divided into three groups with the control hamsters being given daily 9 mg of cholesterol in the diet (CD), whereas the second group being gavage-administered 3 mg of cholesterol three times per day (C-3) and the third group being gavage-administered 9 mg of cholesterol one time per day (C-1). The experiment lasted for 6 weeks. The hamsters were killed under carbon dioxide suffocation. Data demonstrated that plasma TC, non-high-density lipoprotein cholesterol, and triacylglycerols were elevated with the increasing cholesterol intake frequency. Western blotting analyses revealed that the intake frequency had no effect on protein mass of hepatic sterol regulatory element binding protein-2, liver X receptor-α, 3-hydroxy-3-methylglutaryl-CoA reductase, LDL receptor, and cholesterol- 7α-hydroxylase. However, the frequent cholesterol intake down-regulated the mRNA level of hepatic LDL receptor. In contrast, the frequent cholesterol intake up-regulated the mRNA levels of intestinal Niemann-Pick C1-like 1 (NPC1L1), acyl coenzyme A:cholesterol acyltransferase 2 (ACAT2), and microsomal triacylglycerol transport protein (MTP). It was concluded that the cholesterol intake frequency-induced elevation in plasma TC was associated with greater cholesterol absorption, possibly mediated by up-regulation of NPC1L1, ACAT2, and MTP. © 2010 American Chemical Society.

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