Food and Drug Laboratory Research Center

Tehrān, Iran

Food and Drug Laboratory Research Center

Tehrān, Iran
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Javanbakht M.,Amirkabir University of Technology | Moein M.M.,Amirkabir University of Technology | Akbari-adergani B.,Food and Drug Laboratory Research Center
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2012

The applicability of an on-line solid phase extraction method using molecularly imprinted monolithic column was developed for the assay of tramadol (TRD) in urine and plasma samples. The monolithic column was prepared by using TRD as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker and chloroform as the porogen with in situ molecular imprinting polymerization technique. Various parameters affecting the extraction efficiency of the monolithic column were evaluated. Chromatographic analysis of TRD after on-line clean-up of samples was performed by reversed-phase HPLC on an ACE column with ultraviolet detection at 218. nm. The present work was successfully applied for automated simple analysis of TRD in urine and plasma samples with high recoveries between 90.5-93.1% and 93.3-96.0%, respectively. The results revealed that in concentration up to 500. ng/mL of dextromethorphan (DEX), timolol (TMO) and O-desmethyltramadol (M1), the recoveries were not reduced more than 4.3% and 4.0% for plasma and urine samples, respectively. The limit of detection (S/N=3) and limit of quantification (S/N=10) for TRD in urine samples were 0.03. ng/mL and 0.10. ng/mL, and in plasma samples were 0.3 and 1.0. ng/mL, respectively. Inter-column precision of the assays (n=3) for urine and plasma samples at the 100. ng/mL TRD level were 4.0% and 4.2%, respectively. © 2012.


Moein M.M.,Amirkabir University of Technology | Javanbakht M.,Amirkabir University of Technology | Akbari-adergani B.,Food and Drug Laboratory Research Center
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2011

In this paper, a novel method is described for automated determination of dextromethorphan in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and dextromethorphan as template molecule. These imprinted polymers were used as solid-phase extraction sorbent for the extraction of dextromethorphan from human plasma samples. Various parameters affecting the extraction efficiency of the MIP cartridges were evaluated. The high selectivity of the sorbent coupled to the high performance liquid chromatographic system permitted a simple and rapid analysis of this drug in plasma samples with limits of detection (LOD) and quantification (LOQ) of 0.12 ng/mL and 0.35 ng/mL, respectively. The MIP selectivity was evaluated by analyzing of the dextromethorphan in presence of several substances with similar molecular structures and properties. Results from the HPLC analyses showed that the recoveries of dextromethorphan using MIP cartridges from human plasma samples in the range of 1-50 ng/mL were higher than 87%. © 2011 Elsevier B.V.


Moein M.M.,Amirkabir University of Technology | Javanbakht M.,Amirkabir University of Technology | Akbari-Adergani B.,Food and Drug Laboratory Research Center
Talanta | Year: 2014

In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL -1, 0.7 ng mL-1, and 0.03 ng mL-1, 0.1 ng mL-1 were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%. © 2013 Elsevier B.V.


Javanbakht M.,Amirkabir University of Technology | Attaran A.M.,Payamenoor University | Namjumanesh M.H.,Amirkabir University of Technology | Esfandyari-Manesh M.,Amirkabir University of Technology | Akbari-adergani B.,Food and Drug Laboratory Research Center
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2010

In this study, a novel method is described for the determination of tramadol in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and tramadol as template molecule. The novel imprinted polymer was used as a solid-phase extraction (SPE) sorbent for the extraction of tramadol from human plasma and urine. Various parameters affecting the extraction efficiency of the polymer have been evaluated. The optimal conditions for the MIP cartridges were studied. The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of tramadol. The limit of detection (LOD) and limit of quantification (LOQ) for tramadol in urine samples were 1.2 and 3.5 μg L-1, respectively. These limits for tramadol in plasma samples were 3.0 and 8.5 μg L-1, respectively. The recoveries for plasma and urine samples were higher than 91%. © 2010 Elsevier B.V. All rights reserved.


Moein M.M.,Amirkabir University of Technology | Javanbakht M.,Amirkabir University of Technology | Karimi M.,Amirkabir University of Technology | Akbari-Adergani B.,Food and Drug Laboratory Research Center
Talanta | Year: 2014

Sol-gel based molecularly imprinted polymer (MIP) nanofiber was successfully fabricated by electrospinning technique on the surface of a stainless steel bar. The manufactured tool was applied for on-line selective solid phase microextraction (SPME) and determination of acesulfame (ACF) as an artificial sweetener with high performance liquid chromatography (HPLC). The selective ability of method for the extraction of ACF was investigated in the presence of some selected sweeteners such as saccharine (SCH), aspartame (ASP) and caffeine (CAF). Electrospinning of MIP sol-gel solution on the stainless steel bar provided an unbreakable sorbent with high thermal, mechanical, and chemical stability. Moreover, application of the MIP-SPME tool revealed a unique approach for the selective microextraction of the analyte in beverage samples. In this work, 3-(triethoxysilyl)-propylamine (TMSPA) was chosen as a precursor due to its ability to imprint the analyte by hydrogen bonding, Van der Walls, and dipole-dipole interactions. Nylon 6 was also added as a backbone and support for the precursor in which sol could greatly growth during the sol-gel process and makes the solution electrospinable. Various effective parameters in the extraction efficiency of the MIP-SPME tool such as loading time, flow rate, desorption time, selectivity, and the sample volume were evaluated. The linearity for the ACF in beverage sample was in the range of 0.78-100.5 ng mL-1. Limit of detection (LOD) and quantification (LOQ) were 0.23 and 0.78 ng mL-1 respectively. The RSD values (n=5) were all below 3.5% at the 20 ng mL-1 level. © 2014 Elsevier B.V.


Yazdani N.,Tarbiat Modares University | Arzani K.,Tarbiat Modares University | Mostofi Y.,University of Tehran | Shekarchi M.,Food and Drug Laboratory Research Center
Postharvest Biology and Technology | Year: 2011

The effects of 1-methylcyclopropene (1-MCP) on ripening, superficial scald and concentrations of α-farnesene, conjugated trienols (CTols) and antioxidant enzyme activity of 'KS6' Asian pear (Pyrus serotina Rehd.) were studied. 1-MCP treated (2μLL-1) or untreated control fruit were stored at 1°C and 90-95% RH for up to 120 days. 1-MCP treated fruit were firmer than untreated fruit. Application of 1-MCP delayed skin color change. Scald appeared after shorter storage duration and was reduced, but not entirely controlled, with 1-MCP. Accumulation of α-farnesene and oxidation were slower in skin of 1-MCP treated fruit compared with controls. Catalase and peroxidase activities in untreated fruit either increased while activities decreased in 1-MCP treated fruit. Superoxide dismutase activity remained stable. The treatment of Asian pears with 1-MCP followed by cold storage maintained textural characteristics with less scald incidence. © 2010 Elsevier B.V.


Khosrokhavar R.,Food and Drug Laboratory Research Center | Mortazavian A.M.,Shahid Beheshti University
Milchwissenschaft | Year: 2010

Microencapsulation of probiotic microorganisms by using hydrocolloid materials is one of the most efficient techniques recently employed for increasing their viability under detrimental conditions in fermented milk products and under gastrointestinal conditions. The most widely used encapsulating material is Calcium (Ca) alginate. Ca-alginate microcapsules, apart from influencing viability of probiotic microorganisms, might also affect physical, physicochemical and sensory properties of drinks based on fermented milk. In this study, the influence of probiotic-containing microcapsules (Lactobacillus acidophilus LA-5 and Bifidobacterium lactis BB-12) on viscosity, surface tension, phase separation and sensory attributes of Doogh (Iranian drink based on fermented milk) during refrigerated storage period were investigated. Apparent viscosity (instrumental viscosity, but not oral viscosity) in Doogh containing microcapsules in all rotational velocities of viscometer (10, 20 or 50 rpm) except for 100 rpm was significantly higher in comparison to the control treatment. The former treatment exhibited significantly higher serum separation throughout the storage time. After 21 d refrigerated storage, a specific off flavor emerged in Doogh containing microcapsules which intensively enhanced towards the end of storage period (day 42). No other sensorial differences were observed between the two treatments.


Darvish-Damavandi M.,Tehran University of Medical Sciences | Nikfar S.,Tehran University of Medical Sciences | Nikfar S.,Food and Drug Laboratory Research Center | Abdollahi M.,Tehran University of Medical Sciences
World Journal of Gastroenterology | Year: 2010

We evaluated the efficacy and tolerability of mebeverine, a musculotropic antispasmodic agent, in irritable bowel syndrome (IBS) and compared its usual dosages by meta-analysis. Medical databases and all relevant literature were searched from 1965 to June 2009 for any placebo-controlled clinical trials of mebeverine, using search terms such as mebeverine, clinical trials, and IBS. Eight randomized trials met our criteria, including six trials that compared mebeverine with placebo and two that compared mebeverine tablets with capsules. These eight trials included 555 patients randomized to receive either mebeverine or placebo with 352 (63%) women and 203 (37%) men in all subtypes of IBS. The pooled relative risk (RR) for clinical improvement of mebeverine was 1.13 (95% CI: 0.59-2.16, P = 0.7056) and 1.33 (95% CI: 0.92-1.93, P = 0.129) for relief of abdominal pain. The efficacy of mebeverine 200 mg compared to mebeverine 135 mg indicated RRs of 1.12 (95% CI: 0.96-1.3, P = 0.168) for clinical or global improvement and 1.08 (95% CI: 0.87-1.34, P = 0.463) for relief of abdominal pain. Thus, mebeverine is mostly well tolerated with no significant adverse effects; however, its efficacy in global improvement of IBS is not statistically significant. © 2010 Baishideng. All rights reserved.


Hosseini A.,Tehran University of Medical Sciences | Nikfar S.,Tehran University of Medical Sciences | Nikfar S.,Food and Drug Laboratory Research Center | Abdollahi M.,Tehran University of Medical Sciences
Expert Opinion on Biological Therapy | Year: 2012

Introduction: Irritable bowel syndrome (IBS) is a common chronic gastrointestinal (GI) tract disorder with significant disability and a considerable financial burden to health service due to the consumption of resources including investigations, physician time, and cost of treatment. Despite availability of multiple treatment options, there is still poor functional recovery. Areas covered: Probiotics has been investigated as a promising treatment for IBS, and have demonstrated beneficial effects in some patients. There are many clinical trials investigating the therapeutic benefits of probiotics in IBS but most of them are heterogenic in terms of dose or species used and clinical endpoints. However, recent major meta-analyses revealed benefits of probiotics in patients with IBS. Inhibition of binding of pathogenic bacteria to intestinal epithelial cells, enhancing barrier function of intestinal epithelial, acidification of the colon, suppression of the growth of pathogens, modulation of immunity, inhibition of visceral hypersensitivity, alteration in mucosal response to stress, and improvement of bowel dysmotility are among mechanisms that probiotics may act. Most commonly used probiotics come from the genera Bifidobacterium and Lactobacillus but other species are in trial. Expert opinion: Although further studies are still needed, current evidences are almost enough to convince experts that probiotics are efficient in the treatment of IBS. © 2012 Informa UK, Ltd.


Nikfar S.,Tehran University of Medical Sciences | Nikfar S.,Food and Drug Laboratory Research Center
Journal of Medical Hypotheses and Ideas | Year: 2012

Tolerability is an essential part of drug therapy and can affect health and economic outcomes. Withdrawal due to adverse reactions of medicines or lack of effectiveness is a major concern in long-term treatments that influences cost-effectiveness analysis. In case of possibility of stopping and switch to other interventions in decision analysis model, overhead costing may affect results and decision-making processes. Thus, modifying of classic decision analysis model seems to be necessary in such cases. My hypothesis is that by the use of a new decision model that can make links between different Markov-like models accurate cost calculation could be achieved. The appearance of model is going to be like a semicycle net. Considering the probability of switching from one treatment strategy to another, one could give more precise economic evaluation results. In the first step, this model needs to be tested and compared with the conventional model. In the second step, the impact of these differences has to be examined in the practical field of health, drug policy and supply management. By applying this new decision model in total health budget, threshold and its consequences on national health accounts and share of health in gross domestic product should be tested. © 2012 Tehran University of Medical Sciences.

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