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Mousavi S.M.,Tehran University of Medical Sciences | Jahed Khaniki G.,Tehran University of Medical Sciences | Eskandari S.,Food and Drug Control Reference Laboratories Center | Rabiei M.,Food and Drug Control Reference Laboratories Center | And 2 more authors.
Journal of Food Composition and Analysis

Identifying of the species origin in meat and meat products is important for preventing adulteration and protecting consumers in terms of health and religious convictions. Species-specific polymerase chain reaction (PCR) has been known as a suitable method for identifying meat species; however, there is little information on applicability of this method for the detection of fraudulent actions in different food products. This study aimed to evaluate a species-specific PCR assay for the detecting of chicken and donkey meats as adulterants in raw ground meats. Specificity of the primer sets was tested against the target species. The method was applied to the binary meat mixtures of the target species with the detection limits ranged from 0.1% to 10% (w/w). Also, 91 ground beef samples and 53 mixtures of ground beef and lamb samples were collected from local butcheries and tested in order to evaluate the applicability of this method. The oligonucleotide primers amplified mitochondrial DNA sequences and revealed PCR products with expected sizes of 300, 225, 183 and 145 base pair from cattle, sheep, chicken and donkey respectively. PCR assay performed on the experimental meat mixtures showed the detection limit of 0.1% for all primer sets. Results demonstrated that 47.2% and 0.7% of all the samples contained chicken and donkey meats respectively. This method of detection can be applied by quality control laboratories and inspection services to determine adulteration in raw ground meat under certain circumstances. © 2015 Elsevier Inc. Source

Shabani H.,Parsian Bio Technology Laboratory | Mehdizadeh M.,Food and Drug Control Reference Laboratories Center | Mousavi S.M.,Food and Drug Control Reference Laboratories Center | Dezfouli E.A.,Dana Gene Pajouh Laboratory | And 5 more authors.
Food Chemistry

Abstract Consumption of food products derived from porcine sources is strictly prohibited in Islam. Gelatin, mostly derived from bovine and porcine sources, has many applications in the food and pharmaceutical industries. To ensure that food products comply with halal regulations, development of valid and reliable analytical methods is very much required. In this study, a species-specific polymerase chain reaction (PCR) assay using conserved regions of mitochondrial DNA (cytochrome b gene) was performed to evaluate the halal authenticity of gelatin. After isolation of DNA from gelatin powders with known origin, conventional PCR using species-specific primers was carried out on the extracted DNA. The amplified expected PCR products of 212 and 271 bp were observed for porcine and bovine gelatin, respectively. The sensitivity of the method was tested on binary gelatin mixtures containing 0.1%, 1%, 10%, and 100% (w/w) of porcine gelatin within bovine gelatin and vice versa. Although most of the DNA is degraded due to the severe processing steps of gelatin production, the minimum level of 0.1% w/w of both porcine and bovine gelatin was detected. Moreover, eight food products labeled as containing bovine gelatin and eight capsule shells were subjected to PCR examination. The results showed that all samples contained bovine gelatin, and the absence of porcine gelatin was verified. This method of species authenticity is very useful to verify whether gelatin and gelatin-containing food products are derived from halal ingredients. © 2015 Elsevier Ltd. All rights reserved. Source

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