Food and Agricultural Materials Inspection Center

Saitama, Japan

Food and Agricultural Materials Inspection Center

Saitama, Japan
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Ano Y.,University of Tokyo | Ano Y.,Kirin Holdings Co. | Sakudo A.,Osaka University | Kimata T.,University of Tokyo | And 3 more authors.
Neuroscience Letters | Year: 2010

Reactive oxygen species (ROS) play an important role in diverse vital functions including host defense via anti-viral and anti-bacterial effects, but ROS also lead to peroxynitrite and hydroxyl radical production, which are powerful mediators of brain injury in brain inflammation. It is known that NADPH oxidases (NOX) are the major source of ROS. In the present study, NOX2 expression and distribution were examined after intracranial encephalomyocarditis virus B variant (EMCV-B) infection, which causes encephalitis. The reverse transcriptase (RT)-polymerase chain reaction (PCR) and immunohistochemistry showed that the expression and distribution of NOX2 were significantly up-regulated after EMCV-B infection in microglial cells, which invaded into the surrounding regions where neurons were subjected to oxidative stress. These findings suggest that the oxidative stress generated by NOX2 in activated microglial cells damages neurons and that this is an important process in the pathogenesis of EMCV-B infection. © 2009 Elsevier Ireland Ltd. All rights reserved.


Yamamoto T.,Morinaga Institute of Biological Science Inc. | Kato M.,Morinaga Institute of Biological Science Inc. | Endo K.,Morinaga Institute of Biological Science Inc. | Kotoura S.,Marudai Food Co. | Takeda Z.,Food and Agricultural Materials Inspection Center
Journal of Veterinary Medical Science | Year: 2016

A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies directed against a synthetic peptide with an amino-acid sequence related to the C-terminus of bovine myoglobin and the whole molecule of sodium dodecyl sulphate (SDS)-denatured bovine myoglobin was adapted for detecting bovine myoglobin in contaminated feeds. The ELISA employed bovine meat extract of a known myoglobin concentration as a calibration standard and had an limit of detection (LOD) of 3.54 ng/ml and an limit of quantification (LOQ) of 11.0 ng/ml corresponding to 0.022% and 0.067% (wt/wt) bovine meat-and-bone-meal (MBM) mixed in 20-fold-diluted feed extracts, respectively. A cut-off threshold of 20.6 ng/ml bovine myoglobin was set to simplify ELISA and facilitate quick assessment of test results without a tedious calibration process. The ELISA was able to detect bovine MBM in artificially prepared model feeds, mixed botanical feeds, mixed botanical feeds with skimmed milk, fish meal, pork meal and pork/chicken meal at 0.1% (wt/wt). It was also able to detect sheep MBM in test feeds, but showed no reactivity to swine MBM, chicken MBM, skimmed milk or gelatine of bovine origin. The advantages of this method are the quick and easy extraction protocol of proteins from test feeds, using 100 mM sodium sulphide and 0.6% sodium dodecyl sulphate in the extraction solution and the effective detection of bovine and sheep MBM at 0.1% (wt/wt). © 2015 The Japanese Society of Veterinary Science.


Boulange J.,Tokyo University of Agriculture and Technology | Watanabe H.,Tokyo University of Agriculture and Technology | Inao K.,Japan National Institute for Agro - Environmental Sciences | Iwafune T.,Food and Agricultural Materials Inspection Center | And 3 more authors.
Journal of Hydrology | Year: 2014

The objective of this study was to develop, verify, and validate a new GIS-based model for simulating the fate and transport of rice pesticides in river basins. A plot scale model simulating pesticide fate and transport in rice paddies (PCPF-1) was incorporated into the Soil and Water Assessment Tool (SWAT) basin scale water and pollutant transport model. The new combined model, PCPF-1@SWAT model, was first used on some base-case scenarios to verify that the PCPF-1 algorithm and the routing of variables were correctly implemented. Next, the PCPF-1@SWAT model was calibrated and validated on the Sakura River basin (Ibaraki prefecture, Japan) using mefenacet concentrations measured during the rice growing season in 2008. The modeling procedures for simulating pesticide fate and transport in a Japanese river basin were demonstrated by providing model parameters related to hydrology, land use, pesticide fate, and rice field managements methods.The water flows predicted by the PCPF-1@SWAT model in the Sakura River basin were accurate throughout the whole simulation year, with R2 and ENS statistics exceeding 0.74 and 0.71, respectively for daily flow. The use of different seepage rates had appreciable influence on the simulations. High seepage rates gave a slight overestimation of the predicted base flow during the rice growing period, whereas the base flow predictions using lower seepage rates were comparable to measured data.The PCPF-1@SWAT model successfully simulated the fate and transport of mefenacet in the Sakura River in which measured mefenacet concentrations peaked soon after the initial herbicide application in May, and decreased gradually during the months of June and July. Occasional major precipitation events caused the mefenacet concentration in streams to peak quickly due to a corresponding loss of mefenacet from paddy areas, and then rapidly decrease due to dilution by excess rainfall discharge. The simulation using a seepage rate of 0.12cmday-1 had the most accurate prediction of mefenacet concentration in river water with an R2 of 0.61 and an ENS of 0.65. © 2014 Elsevier B.V.


Hiraoka H.,Food and Agricultural Materials Inspection Center | Goto T.,Shinshu University
Animal Feed Science and Technology | Year: 2010

Organic acids and some inorganic anions may have an effect on the nutritive quality of silage. To facilitate the simultaneous and quantitative measurement of nine organic acids (formate, acetate, propionate, butyrate, n-valerate, iso-valerate, n-hexanate, citrate and lactate) and three inorganic anions (nitrate, nitrite and chloride) in silage, we optimized an analysis method using capillary zone electrophoresis (CZE) with a photodiode-array detector (PDA). We then validated the optimized method in a collaborative study. Samples were extracted with water, purified through a cartridge column to remove interferences, diluted with water, filtered with a centrifugal filter and applied on the CZE. The separation was carried out with a fused-silica capillary column at 30. kV. A wavelength of 350. nm was selected for indirect UV detection and a wavelength of 275. nm was selected as a reference. A 30. mM 2,6-pyridinedicarboxylic acid (PDC) solution was used as a background electrolyte (BGE) at pH 12 with 0.5. mM of cetyltrimethylammonium hydroxide (CTAH).For the recovery study, the silage was spiked with 3.0 or 6.0g/kg of each of the nine organic acids and three inorganic anions. The mean recoveries of the acids were 0.996-1.097 and the coefficient of variation for the repeatability (CV) was within 0.071. The collaborative study included five laboratories. Grass silage was spiked with 7.0g/kg of each analyte. The mean recoveries of the acids were 0.843-1.143, the coefficients of variation for the repeatability and reproducibility (CVr and CVR) were zero to 0.058 and 0.033-0.081, respectively, and the HorRat values were 0.8-1.9. From the measurements of farm silage samples, chloride, nitrate, formate, citrate, acetate, lactate, butyrate and iso-valerate were detected, and their mean ranges were 2.7-4.4, 0-0.9, 0-2.6, 0-1.8, 0.7-5.5, 0-12.3, 0-3.4 and 0-2.4g/kg, respectively, and the coefficients of variation for the repeatability were within 0.087, 0.079, 0.083, 0.095, 0.091, 0.070, 0.103 and 0.037, respectively. Over a long-term storage period of nine months, nitrate and citrate decreased. The acetate and lactate contents were higher in the high-moisture silage than the low-moisture silage. © 2010 Elsevier B.V.


Iwafune T.,Food and Agricultural Materials Inspection Center | Ogino T.,Food and Agricultural Materials Inspection Center | Watanabe E.,Japan National Institute for Agro - Environmental Sciences
Journal of Agricultural and Food Chemistry | Year: 2014

This paper proposes an environmentally friendly method involving water-based extraction of the samples, cleanup of the extracts by solid-phase extraction, and subsequent liquid chromatography coupled with tandem mass spectrometry, which was used for simultaneous determination of seven hydrophilic neonicotinoid insecticides as well as their metabolites in agricultural samples. The effects of sample matrix on detection of the target compounds were negligibly small. Mean recoveries obtained at spiked concentrations between 0.01 and 1.00 mg/kg were 71.2-122.3% with relative standard deviations of ≥7.5%. When the method was applied to crop samples sprayed with commercial formulations of the target compounds, the residual concentrations of the compounds determined by the proposed method (0.015-0.27 mg/kg in green peppers and 0.017-0.31 mg/kg in tomatoes) were equivalent to those determined by the official Japanese method (0.017-0.26 mg/kg in green peppers and 0.013-0.30 mg/kg in tomatoes). © 2014 American Chemical Society.


Nagai T.,Japan National Institute for Agro - Environmental Sciences | Ishihara S.,Food and Agricultural Materials Inspection Center | Yokoyama A.,Japan National Institute for Agro - Environmental Sciences | Iwafune T.,Food and Agricultural Materials Inspection Center
Environmental Toxicology and Chemistry | Year: 2011

Paddy herbicides are a high-risk concern for aquatic plants, including algae, because they easily flow out from paddy fields into rivers, with toxic effects. The effect on algal population dynamics, including population recovery after timed exposure, must be assessed. Therefore, we demonstrated concentration-response relationships of four paddy herbicides for algal growth inhibition and mortality, and the relationship between the effect on algal cell viability and population recovery following exposure. We used SYTOX Green dye assay and flow cytometry to assess cell viability of the alga Pseudokirchneriella subcapitata. Live cells could be clearly distinguished from dead cells during herbicide exposure. Our results showed that pretilachlor and quinoclamine had both algicidal and algistatic effects, whereas bensulfuron-methyl only had an algistatic effect, and pentoxazone only had an algicidal effect. Then, a population recovery test following a 72-h exposure was conducted. The algal population recovered in all tests, but the periods required for recovery differed among exposure concentrations and herbicides. The periods required for recovery were inconsistent with the dead cell ratio at the beginning of the recovery test; that is, population recovery could not be described only by cell viability. Consequently, the temporal effect of herbicides and subsequent recovery of the algal population could be described not only by the toxicity characteristics but also by toxicokinetics, such as rate of uptake, transport to the target site, and elimination of the substance from algal cells. © 2011 SETAC.


Iguchi J.,Food and Agricultural Materials Inspection Center | Takashima Y.,Food and Agricultural Materials Inspection Center | Namikoshi A.,Food and Agricultural Materials Inspection Center | Yamashita M.,Japan National Research Institute of Fisheries Science
Fisheries Science | Year: 2012

The complete nucleotide sequences of mitochondrial DNA (mtDNA) from four Seriola spp. (S. quinqueradiata, S. lalandi, S. dumerili, and S. rivoliana) were determined with the aim of developing a species identification analysis method for discriminating between commercially important Seriola spp. and other related species. In addition, the nucleotide sequences of the mitochondrial cytochrome b gene (Cytb) from five related but less expensive species in terms of market value (Seriolella brama, S. caerulea, S. punctata, Hyperoglyphejaponica, and Rachycentroncanadum), which are often used as substitutes for Seriola spp., were determined. Restriction enzyme sites were examined by comparing the nucleotide sequences, and species-specific primers were designed for PCR-based restriction fragment length polymorphism (RFLP) analysis. Based on the results of the PCR amplification studies, the four Seriola spp. and the five related species tested could be categorized into three groups according to their PCR product pattern: a 373-bp product from the four Seriola spp., a 513-bp product from three Seriolella spp. and H. japonica, and a 204-bp product from R. canadum. In addition, RFLP analysis of the PCR products was able to differentiate these fish species. © 2011 The Japanese Society of Fisheries Science.


Phong T.K.,Kyushu University | Vu S.H.,Ministry of Agriculture and Rural Development | Ishihara S.,Food and Agricultural Materials Inspection Center | Hiramatsu K.,Kyushu University | Watanabe H.,Tokyo University of Agriculture and Technology
Pest Management Science | Year: 2011

Background: Monitoring studies revealed high concentrations of pesticides in the drainage canal of paddy fields. It is important to have a way to predict these concentrations in different management scenarios as an assessment tool. A simulation model for predicting the pesticide concentration in a paddy block (PCPF-B) was evaluated and then used to assess the effect of water management practices for controlling pesticide runoff from paddy fields.Results: The PCPF-B model achieved an acceptable performance. The model was applied to a constrained probabilistic approach using the Monte Carlo technique to evaluate the best management practices for reducing runoff of pretilachlor into the canal. The probabilistic model predictions using actual data of pesticide use and hydrological data in the canal showed that the water holding period (WHP) and the excess water storage depth (EWSD) effectively reduced the loss and concentration of pretilachlor from paddy fields to the drainage canal. The WHP also reduced the timespan of pesticide exposure in the drainage canal.Conclusions: It is recommended that: (1) the WHP be applied for as long as possible, but for at least 7 days, depending on the pesticide and field conditions; (2) an EWSD greater than 2 cm be maintained to store substantial rainfall in order to prevent paddy runoff, especially during the WHP. © 2010 Society of Chemical Industry.


Baba R.,Nagoya University | Kimura M.,Nagoya University | Kimura M.,Food and Agricultural Materials Inspection Center | Asakawa S.,Nagoya University | Watanabe T.,Nagoya University
FEMS Microbiology Letters | Year: 2014

Hydrogen (H2) is one of the most important intermediates in the anaerobic decomposition of organic matter. Although the microorganisms consuming H2 in anaerobic environments have been well documented, those producing H2 are not well known. In this study, we elucidated potential members of H2-producing bacteria in a paddy field soil using clone library analysis of [FeFe]-hydrogenase genes. The [FeFe]-hydrogenase is an enzyme involved in H2 metabolism, especially in H2 production. A suitable primer set was selected based on the preliminary clone library analysis performed using three primer sets designed for the [FeFe]-hydrogenase genes. Soil collected in flooded and drained periods was used to examine the dominant [FeFe]-hydrogenase genes in the paddy soil bacteria. In total, 115 and 108 clones were analyzed from the flooded and drained paddy field soils, respectively. Homology and phylogenetic analysis of the clones showed the presence of diverse [FeFe]-hydrogenase genes mainly related to Firmicutes, Deltaproteobacteria, and Chloroflexi. Predominance of Deltaproteobacteria and Chloroflexi suggests that the distinct bacterial community possessed [FeFe]-hydrogenase genes in the paddy field soil. Our study revealed the potential members of H2-producing bacteria in the paddy field soil based on their genetic diversity and the distinctiveness of the [FeFe]-hydrogenase genes. © 2013 Federation of European Microbiological Societies.


Hakoda A.,Food and Agricultural Materials Inspection Center
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2010

Validated analytical methods have been adopted in Codex Alimentarius Commission for international regulatory analysis. They also have been needed in the Japanese Agricultural Standard (JAS) because of increased international business through the import and export. In this regard, the Food and Agricultural Materials Inspection Center (FAMIC) have designed the candidate analytical methods and conducted their interlaboratory studies in commission of the Minister of Agriculture, Forestry and Fisheries. All procedures were performed under the international harmonized protocol of method-performance study. In this article, the general procedure for analytical methods in the JAS is summarized. Practical interlaboratory studies, performed at FAMIC, are also introduced on determination of crude protein and ash in macaroni products.

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