Fondazione Studio Diagnostica Nucleare SDN

Napoli, Italy

Fondazione Studio Diagnostica Nucleare SDN

Napoli, Italy

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de Nigris F.,The Second University of Naples | Rienzo M.,The Second University of Naples | Sessa M.,The Second University of Naples | Infante T.,Fondazione Studio Diagnostica Nucleare SDN | And 6 more authors.
Journal of Cellular Physiology | Year: 2012

Oxidation and glycation enhance foam cell formation via MAPK/JNK in euglycemic and diabetic subjects. Here, we investigated the effects of glycated and oxidized LDL (glc-oxLDL) on MAPK-ERK and JNK signaling pathways using human coronary smooth muscle cells. Glc-oxLDL induced a broad cascade of MAPK/JNK-dependent signaling transduction pathways and the AP-1 complex. In glc-oxLDL treated coronary arterioles, tumor necrosis factor (TNF) α increased JNK phosphorylation, whereas protein kinase inhibitor dimethylaminopurine (DMAP) prevented the TNF-induced increase in JNK phosphorylation. The role of MKK4 and JNK were then investigated in vivo, using apolipoprotein E knockout (ApoE-/-) mice. Peritoneal macrophages, isolated from spontaneously hyperlipidemic but euglycemic mice showed increases in both proteins and phosphorylated proteins. Compared to streptozotocin-treated diabetic C57BL6 and nondiabetic C57BL6 Wt mice, in streptozotocin-diabetic ApoE-/- mice, the increment of foam cell formation corresponded to an increment of phosphorylation of JNK1, JNK2, and MMK4. Thus, we provide a first line of evidence that MAPK-ERK/JNK pathways are involved in vascular damage induced by glycoxidation. © 2012 Wiley Periodicals, Inc.


de Nigris F.,The Second University of Naples | Mancini F.P.,University of Sannio | Schiano C.,The Second University of Naples | Infante T.,Fondazione Studio Diagnostica Nucleare SDN | And 6 more authors.
Journal of Cellular Physiology | Year: 2013

Understanding the mechanisms inducing endothelial cell (EC) proliferation following tumor microenvironment stimuli may be important for the development of antiangiogenic therapies. Here, we show that cyclin-dependent kinase 2 and 5 (Cdk2, Cdk5) are important mediators of neoangiogenesis in in vitro and in vivo systems. Furthermore, we demonstrate that a specific Yin Yang 1 (YY1) protein-dependent signal from osteosarcoma (SaOS) cells determines proliferation of human aortic endothelial cells (HAECs). Following tumor cell stimuli, HAECs overexpress Cdk2 and Cdk5, display increased Cdk2 activity, undergo enhanced proliferation, and form capillary-like structures. Moreover, Roscovitine, an inhibitor of Cdks, blunted overexpression of Cdk2 and Cdk5 and Cdk2 activity induced by the YY1-dependent signal secreted by SaOS cells. Furthermore, Roscovitine decreased HAEC proliferation and angiogenesis (the latter by 70% in in vitro and 50% in in vivo systems; P<0.01 vs. control). Finally, the finding that Roscovitine triggers apoptosis in SaOS cells as well as in HAECs by activating caspase-3/7 indicates multiple mechanisms for the potential antitumoral effect of Roscovitine. Present work suggests that Cdk2 and Cdk5 might be pharmacologically accessible targets for both antiangiogenic and antitumor therapy. © 2012 Wiley Periodicals, Inc.


Napoli C.,The Second University of Naples | Zullo A.,University of Sannio | Picascia A.,The Second University of Naples | Infante T.,Fondazione Studio Diagnostica Nucleare SDN | And 2 more authors.
Journal of Cellular Biochemistry | Year: 2013

In recent years, the diagnosis of cardiovascular disease (CVD) has increased its potential, also thanks to mass spectrometry (MS) proteomics. Modern MS proteomics tools permit analyzing a variety of biological samples, ranging from single cells to tissues and body fluids, like plasma and urine. This approach enhances the search for informative biomarkers in biological samples from apparently healthy individuals or patients, thus allowing an earlier and more precise diagnosis and a deeper comprehension of pathogenesis, development and outcome of CVD to further reduce the enormous burden of this disease on public health. In fact, many differences in protein expression between CVD-affected and healthy subjects have been detected, but only a few of them have been useful to establish clinical biomarkers because they did not pass the verification and validation tests. For a concrete clinical support of MS proteomics to CVD, it is, therefore, necessary to: ameliorate the resolution, sensitivity, specificity, throughput, precision, and accuracy of MS platform components; standardize procedures for sample collection, preparation, and analysis; lower the costs of the analyses; reduce the time of biomarker verification and validation. At the same time, it will be fundamental, for the future perspectives of proteomics in clinical trials, to define the normal protein maps and the global patterns of normal protein levels, as well as those specific for the different expressions of CVD. © 2012 Wiley Periodicals.


Schiano C.,The Second University of Naples | Grimaldi V.,The Second University of Naples | Casamassimi A.,The Second University of Naples | Infante T.,Fondazione Studio Diagnostica Nucleare SDN | And 3 more authors.
Medical Oncology | Year: 2012

The CD146 cell membrane adhesion molecule is highly expressed on the cell surface of several tumours. The level of its expression has been found to correlate directly with tumour progression and metastatic potential, thus establishing CD146 as an important candidate of tumour growth and metastasis. In order to characterize its expression in human osteosarcoma (OS) cell lines, we have examined the CD146 expression at protein and RNA levels in both normal and tumour osteoblast-like cell lines by several methods. Our results indicate that CD146 protein is expressed at low levels in normal osteoblast cells whereas it is highly expressed in all OS cell lines analysed, (SaOS, MG-63, U-2OS). Moreover, CD146 overexpression was partially reduced in shYY1 cells, where the Yin Yang 1 transcription factor, also found over-expressed in human OS cells, has been silenced. © 2012 Springer Science+Business Media, LLC.


Grimaldi V.,The Second University of Naples | Cesario E.,The Second University of Naples | Casamassimi A.,The Second University of Naples | Infante T.,Fondazione Studio Diagnostica Nucleare SDN | Napoli C.,The Second University of Naples
Immunology, Endocrine and Metabolic Agents in Medicinal Chemistry | Year: 2012

The relevance of anti-human leucocyte antigen (anti-HLA) antibodies in the outcome of organ transplantation has been recognized in the past 40 years. Detection of these antibodies in sensitized recipients plays a pivotal role in this issue. Flow cytometry analysis is one of the choice cell-based techniques together with the pioneer complementdependent cytotoxicity crossmatch. Here, we discuss the advantages and limits of these cell-based assays and their use in the clinical laboratory of immunology of transplantation. Moreover, we compared this approach to other beads-based detection assays introduced more recently in the laboratory practice. © 2012 Bentham Science Publishers.


Casamassimi A.,The Second University of Naples | Grimaldi V.,The Second University of Naples | Infante T.,Fondazione Studio Diagnostica Nucleare SDN | Al-Omran M.,King Saud University | And 2 more authors.
Cardiovascular and Hematological Agents in Medicinal Chemistry | Year: 2012

The following paper is an overview on stem cells therapy in patients with peripheral vascular diseases. Recent research shows the ability of stem cells to develop and strengthen the collateral network in ischemic legs. Here, we discuss this clinical and therapeutic approach. To date, research has been mainly focused on patients with ischemic ulcers without possibility of revascularization. Non-invasive stem cell therapy has been proposed as an alternative to the amputation of such patients, but when the ulcers appear it is sometime too late. In our point of view, the selection of patients is a very important issue and we believe that the best candidate for this treatment is the patient with intermittent claudication before the development of ulcers. This choice could allow the optimization of results by the type of treated patients and not only by the type of infused cells. Indeed, several variables still remain to be elucidated for stem cell therapy, including the type of cells to be used, the infusion route, and more importantly, the stage of patients to be treated. © 2012 Bentham Science Publishers.

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