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Paone G.,University of Rome La Sapienza | Conti V.,University of Rome La Sapienza | Leone A.,Pathology Unit | Schmid G.,IRCCS Fondazione Don Carlo Gnocchi ONLUS | And 3 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2011

Background and Objectives: Human Neutrophil Peptides (HNP) are major neutrophils' products which may contribute to the airway inflammation and lung remodelling during chronic obstructive pulmonary disease (COPD). We aimed to assess whether HNP sputum concentrations could be used as indicators of airway inflammation and progression towards pulmonary functional impairment, and correlate with the degree of airways obstruction. Materials and Methods: We measured, by ELISA tests, HNP concentrations from 37 symptomatic smokers and 34 COPD patients. All participants underwent pulmonary function tests. Sputum samples were collected at the enrolment, and 6 months after smoking cessation. Differences between groups and correlation coefficients between variables were determined using non parametric tests. Results: Sputum HNP concentrations were higher in COPD patients as compared to symptomatic smokers (14±1.5 μg/ml vs 1.6±0.4 μg/ml; p<0.0001). Among COPD patients HNP concentrations were higher in individuals with severe obstruction than in patients with mild to moderate COPD (19.9±2.3 μg/ml vs 10.3±0.8 μg/ml, p=0.003). A negative correlation was observed between HNP levels and FEV1 (rho= -0.38, p=0.02), and FEV1/FVC (rho= -0.42, p=0.01). No differences were found in HNP levels before and after 6 months of smoking withdrawal (1.1 μg/ml±0.3 vs 1.1 μg/ml±0.3 for symptomatic smokers, p=0.9, and 14.4 μg/ml± 1 vs 16 μg/ml±1.1 for COPD, p=0.6). Discussion: Sputum levels of HNP may represent a marker of severity of functional impairment in COPD. Our data support the hypothesis that HNP may have a role in smoking-and COPD-related lung inflammation. Source


Paone G.,University of Rome La Sapienza | Conti V.,University of Rome La Sapienza | Vestri A.,University of Rome La Sapienza | Leone A.,Pathology Unit | And 6 more authors.
Disease Markers | Year: 2011

The pivotal role of neutrophils and macrophages in smoking-related lung inflammation and COPD development is well-established. We aimed to assess whether sputum concentrations of Human Neutrophil Peptides (HNP), Neutrophil Elastase (NE), Interleukin-8 (IL-8), and Metalloproteinase-9 (MMP-9), major products of neutrophils and macrophages, could be used to trace airway inflammation and progression towards pulmonary functional impairment characteristic of COPD. Forty-two symptomatic smokers and 42 COPD patients underwent pulmonary function tests; sputum samples were collected at enrolment, and 6 months after smoking cessation. HNP, NE, IL-8, MMP-9 levels were increased in individuals with COPD (p < 0.0001). HNP and NE concentrations were higher in patients with severe airways obstruction, as compared to patients with mild-to-moderate COPD (p =0.002). A negative correlation was observed between FEV 1 and HNP, NE and IL-8 levels (p < 0.01), between FEV 1FVC and HNP, NE and IL-8 levels (p< 0.01), and between NE enrolment levels and FEV 1 decline after 2 years (p =0.04). ROC analysis, to discriminate symptomatic smokers and COPD patients, showed the following AUCs: for HNP 0.92; for NE 0.81; for IL-8 0.89; for MMP-9 0.81; for HNP, IL-8 and MMP-9 considered together 0.981. The data suggest that the measurement of sputum markers may have an important role in clinical practice for monitoring COPD. © 2011 - IOS Press and the authors. All rights reserved. Source


Paone G.,University of Rome La Sapienza | Conti V.,IRCCS San Raffaele Pisana | Biondi-Zoccai G.,University of Rome La Sapienza | De Falco E.,University of Rome La Sapienza | And 9 more authors.
Mediators of Inflammation | Year: 2014

Background. Long-term home noninvasive mechanical ventilation (NIV) is beneficial in COPD but its impact on inflammation is unknown. We assessed the hypothesis that NIV modulates systemic and pulmonary inflammatory biomarkers in stable COPD. Methods. Among 610 patients referred for NIV, we shortlisted those undergoing NIV versus oxygen therapy alone, excluding subjects with comorbidities or non-COPD conditions. Sputum and blood samples were collected after 3 months of clinical stability and analyzed for levels of human neutrophil peptides (HNP), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNF-alpha). Patients underwent a two-year follow-up. Unadjusted, propensity-matched, and pH-stratified analyses were performed. Results. Ninety-three patients were included (48 NIV, 45 oxygen), with analogous baseline features. Sputum analysis showed similar HNP, IL-6, IL-10, and TNF-alpha levels (P > 0.5). Conversely, NIV group exhibited higher HNP and IL-6 systemic levels (P < 0.001) and lower IL-10 concentrations (P < 0.001). Subjects undergoing NIV had a significant reduction of rehospitalizations during follow-up compared to oxygen group (P = 0.005). These findings were confirmed after propensity matching and pH stratification. Conclusions. These findings challenge prior paradigms based on the assumption that pulmonary inflammation is per se detrimental. NIV beneficial impact on lung mechanics may overcome the potential unfavorable effects of an increased inflammatory state. © 2014 Gregorino Paone et al. Source


Salatino C.,IRCCS Fondazione Don Carlo Gnocchi ONLUS | Gower V.,IRCCS Fondazione Don Carlo Gnocchi ONLUS | Ghrissi M.,Robosoft Services Robots | Tapus A.,ENSTA ParisTech | And 7 more authors.
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2016

Mild cognitive impairment (MCI) is a state related to ageing, and sometimes evolves to dementia. As there is no pharmacological treatment for MCI, a non-pharmacological approach is very important. The use of Information and Communication Technologies (ICT) in care and assistance services for elderly people increases their chances of prolonging independence thanks to better cognitive efficiency. Robots are seen to have the potential to support the care and independence of elderly people. The project ENRICHME (funded by the EU H2020 Programme) focuses on developing and testing technologies for supporting elderly people with MCI in their living environment for a long time. This paper describes the results of the activities conducted during the first year of the ENRICHME project, in particular the definition of user needs and requirements and the resulting system architecture. © Springer International Publishing Switzerland 2016. Source


Mehn D.,IRCCS Fondazione Don Carlo Gnocchi ONLUS | Morasso C.,IRCCS Fondazione Don Carlo Gnocchi ONLUS | Vanna R.,IRCCS Fondazione Don Carlo Gnocchi ONLUS | Schiumarini D.,IRCCS Fondazione Don Carlo Gnocchi ONLUS | And 3 more authors.
BioNanoScience | Year: 2014

We present here the surface-enhanced Raman spectroscopic (SERS)-based detection of the Wilm's tumor gene (WT1) sequence using dye-labeled reporter oligonucleotide and magnetic core @ gold shell nanoparticles. Thiolated single-stranded DNA (ssDNA) complementers of the WT1 sequence were used to functionalize the gold shell with capture oligonucleotides in a facile and fast two-step method. The signal amplification performance of the core @ shell colloidal SERS substrate was tested using malachite green as label dye. The Raman signal enhancing efficacy of the magnetic core @ gold shell nanomaterial was compared with the efficacy of spherical gold particles produced using the conventional citrate reduction method. The core @ shell particles were found to be superior both regarding robustness in SERS and facile separation in a heterogeneous reaction system. The core @ shell particles functionalized with target specific oligonucleotide were able to capture the WT1 target and worked as Raman signal amplifiers in our assay system. The good physicochemical characteristics of these particles and the sensitivity observed in SERS experiments allow us to expect good performance in the further development steps of a novel, fast and reliable spectroscopic method for WT1 detection in minimal residual disease patients. © 2014 Springer Science+Business Media New York. Source

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