FMC BioPolymer AS NovaMatrix

Sandvika, Norway

FMC BioPolymer AS NovaMatrix

Sandvika, Norway

Time filter

Source Type

Andersen T.,FMC BioPolymer AS NovaMatrix | Andersen T.,Norwegian University of Science and Technology | Melvik J.E.,FMC BioPolymer AS NovaMatrix | Gaserod O.,FMC BioPolymer AS NovaMatrix | And 2 more authors.
Biomacromolecules | Year: 2012

Alginates in the format of scaffolds provide important functions as materials for cell encapsulation, drug delivery, tissue engineering and wound healing among others. The method for preparation of alginate-based foams presented here is based on homogeneous, ionotropic gelation of aerated alginate solutions, followed by air drying. The method allows higher flexibility and better control of the pore structure, hydration properties and mechanical integrity compared to foams prepared by other techniques. The main variables for tailoring hydrogel properties include operational parameters such as degree of aeration and mixing times and concentration of alginate, as well as macromolecular properties such as the type of alginate (chemical composition and molecular weight distribution). Exposure of foams to γ-irradiation resulted in a dose-dependent (0-30 kGy) reduction in molecular weight of the alginate and a corresponding reduction in tensile strength of the foams. © 2012 American Chemical Society.


Andersen T.,FMC BioPolymer AS NovaMatrix | Andersen T.,Norwegian University of Science and Technology | Markussen C.,FMC BioPolymer AS NovaMatrix | Dornish M.,FMC BioPolymer AS NovaMatrix | And 4 more authors.
Tissue Engineering - Part A | Year: 2014

Essential cellular functions are often lost under culture in traditional two-dimensional (2D) systems. Therefore, biologically more realistic three-dimensional (3D) cell culture systems are needed that provide mechanical and biochemical cues which may otherwise be unavailable in 2D. For the present study, an alginate-based hydrogel system was used in which cells in an alginate solution were seeded onto dried alginate foams. A uniform distribution of NIH:3T3 and NHIK 3025 cells entrapped within the foam was achieved by in situ gelation induced by calcium ions integrated in the foam. The seeding efficiency of the cells was about 100% for cells added in a seeding solution containing 0.1-1.0% alginate compared with 18% when seeded without alginate. The NHIK 3025 cells were allowed to proliferate and form multi-cellular structures inside the transparent gel that were later vital stained and evaluated by confocal microcopy. Gels were de-gelled at different time points to isolate the multi-cellular structures and to determine the spheroid growth rate. It was also demonstrated that the mechanical properties of the gel could largely be varied through selection of type and concentration of the applied alginate and by immersing the already gelled disks in solutions providing additional gel-forming ions. Cells can efficiently be incorporated into the gel, and single cells and multi-cellular structures that may be formed inside can be retrieved without influencing cell viability or contaminating the sample with enzymes. The data show that the current system may overcome some limitations of current 3D scaffolds such as cell retrieval and in situ cell staining and imaging. © 2014 Mary Ann Liebert, Inc.


Andersen T.,FMC BioPolymer AS NovaMatrix | Andersen T.,Norwegian University of Science and Technology | Melvik J.E.,FMC BioPolymer AS NovaMatrix | Gaserod O.,FMC BioPolymer AS NovaMatrix | And 2 more authors.
Carbohydrate Polymers | Year: 2014

A new and flexible method for preparation of dry macroporous alginate foams with the capability of absorbing physiological solutions has been developed, which may find use within areas such as wound healing, cell culture, drug delivery and tissue engineering. The present study demonstrates how the gelation rate of the alginate and degree of ionic crosslinking can be utilized to control the physical foam properties. The rate of released Ca 2+/Sr2+ gelling ions available for interaction with the alginate was influenced by the concentration and physical characteristics of CaCO3/SrCO3 particles. The method of preparation of such foams allows, as described herein, tailoring of the pore structure, hydration properties and mechanical integrity in a manner not possible by other techniques. © 2013 Elsevier Ltd. All rights reserved.


Kristiansen K.A.,Norwegian University of Science and Technology | Tomren H.B.,Norwegian University of Science and Technology | Tomren H.B.,FMC BioPolymer AS NovaMatrix | Christensen B.E.,Norwegian University of Science and Technology
Carbohydrate Polymers | Year: 2011

Limited periodate oxidation (0-8%) of alginates results in enhanced degradability, extending the range of applications of alginates as biomaterials, e.g. in tissue engineering. Oxidation produces dialdehydes that are highly sensitive to alkaline β-elimination, even under physiological conditions (pH 7.4, 37°C). Although all periodate is consumed during limited oxidation, not all of the resulting dialdehydes are equally degradable, leading to a characteristic level off in molecular weight upon prolonged degradation. A significant fraction (20-50%) of the oxidized residues were resistant even at pH 10.4. We tentatively assign this finding to the presence of particularly stable intramolecular hemiacetals known to form in periodate oxidized alginates (POA). The enhanced degradation of POA persists into the gel state, allowing tailoring of more biodegradable alginate gels. The activation energy for β-elimination of dialdehydes was 88 kJ/mol compared to 113 kJ/mol for unoxidized residues, presumably reflecting the absence of strain in the non-cyclic transition state of the former. © 2011 Elsevier Ltd. All rights reserved.


PubMed | Norwegian University of Science and Technology and FMC BioPolymer AS NovaMatrix
Type: Journal Article | Journal: Carbohydrate polymers | Year: 2015

The kinetics and mechanisms of depolymerization of aqueous chitosan and alginate solutions at elevated temperatures have been investigated. Chitosan salts of different degree of acetylation (FA), type of counterions (-glutamate, -chloride) and degree of purity were studied. One commercially available highly purified sodium alginate sample with high content of guluronic acid (G) was also studied. Furthermore, the influence of oxygen, H(+) and OH(-) ions on the initial depolymerization rates was investigated. Depolymerization kinetics was followed by measuring the time courses of the apparent viscosity and the intrinsic viscosity. The initial rate constants for depolymerization were determined from the intrinsic viscosity data converted to a quantity proportional to the fraction of bonds broken. The activation energies of the chitosan chloride and chitosan glutamate solutions with pH close to 5 and the same degree of acetylation, FA=0.14, were determined from the initial rate constants to be 7613kJ/mol and 8011kJ/mol, respectively. The results reported herein suggest that the stability of aqueous chitosan and alginate solutions at pH values 5-8 will be influenced by oxidative-reductive depolymerization (ORD) as the primary mechanism as long as transition metal ions are presented in the samples. Acid - and alkaline depolymerization will be the primary mechanisms for highly purified samples.


PubMed | FMC BioPolymer AS NovaMatrix
Type: Journal Article | Journal: Biomacromolecules | Year: 2012

Alginates in the format of scaffolds provide important functions as materials for cell encapsulation, drug delivery, tissue engineering and wound healing among others. The method for preparation of alginate-based foams presented here is based on homogeneous, ionotropic gelation of aerated alginate solutions, followed by air drying. The method allows higher flexibility and better control of the pore structure, hydration properties and mechanical integrity compared to foams prepared by other techniques. The main variables for tailoring hydrogel properties include operational parameters such as degree of aeration and mixing times and concentration of alginate, as well as macromolecular properties such as the type of alginate (chemical composition and molecular weight distribution). Exposure of foams to -irradiation resulted in a dose-dependent (0-30 kGy) reduction in molecular weight of the alginate and a corresponding reduction in tensile strength of the foams.

Loading FMC BioPolymer AS NovaMatrix collaborators
Loading FMC BioPolymer AS NovaMatrix collaborators