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Mahamuni S.P.,P.A. College | Khose R.D.,P.A. College | Menaa F.,Fluorotronics Inc. | Badole S.L.,P.A. College
BioMedicine (Netherlands) | Year: 2012

Hyperlipidemia is a metabolic syndrome characterized by diverse lipid profiles (e.g. hypercholesterolemia, hypertriglyceridemia, and familial combined hyperlipidemia) and may have significant adverse effects on health (e.g. atherosclerosis, cardiovascular diseases, diabetes, insulin resistance, obesity). Both genetic and environmental components are associated with hyperlipidemia sub-types. Effective drugs targeting hyperlipidemia sub-types are thus required. In the present review, we mainly focus on types of hyperlipidemia, digestion, and absorption of lipids as well as on their consequences on human health and on potential effective drug targets against hyperlipidemia. Omega-3 fatty acids have favorable effect on reducing postprandial triglyceride levels and will be beneficial if combined with statins. © 2012.

Shahrokhi S.,Lorestan University of Medical Sciences | Daneshmandi S.,Tarbiat Modares University | Menaa F.,Fluorotronics Inc.
Human Gene Therapy | Year: 2014

The interaction between mesenchymal stem cells (MSCs) and dendritic cells (DCs) affects T cell development and function. Further, the chemotactic capacity of MSCs, their interaction with the tumor microenvironment, and the intervention of immune-stimulatory molecules suggest possible exploitation of tumor necrosis factor-α (TNF-α) and CD40 ligand (CD40L) to genetically modify MSCs for enhanced cancer therapy. Both DCs and MSCs were isolated from BALB/c mice. DCs were then cocultured with MSCs transduced with TNF-α and/or CD40L [(TNF-α/CD40L)-MSCs]. Major DCs' maturation markers, DC and T cell cytokines such as interleukin-4,-6,-10,-12, TNF-α, tumor growth factor-β, as well as T cell proliferation, were assessed. Meantime, a BALB/c mouse breast tumor model was inducted by injecting 4T1 cells subcutaneously. Mice (n=10) in each well-defined test groups (n=13) were cotreated with DCs and/or (TNF-α/CD40L)-MSCs. The controls included untreated, empty vector-MSC, DC-lipopolysaccharide, and immature DC mouse groups. Eventually, cytokine levels from murine splenocytes, as well as tumor volume and survival of mice, were assessed. Compared with the corresponding controls, both in vitro and in vivo analyses showed induction of T helper 1 (Th1) as well as suppression of Th2 and Treg responses in test groups, which led to a valuable antitumor immune response. Further, the longest mouse survival was observed in mouse groups that were administered with DCs plus (TNF-α/CD40L)-MSCs. In our experimental setting, the present pioneered study demonstrates that concomitant genetic modification of MSCs with TNF-α and CD40L optimized the antitumor immunity response in the presence of DCs, meantime increasing the mouse lifespan. © Copyright 2014, Mary Ann Liebert, Inc. 2014.

Bakhsh S.,Gomal University | Khan G.M.,Quaid-i-Azam University | Menaa F.,Fluorotronics Inc. | Khan B.A.,Gomal University | Khan B.A.,Kampala International University
Pakistan Journal of Pharmaceutical Sciences | Year: 2015

The present study was conducted to formulate controlled release dosage forms containing Ibuprofen with Eudragit® S 100 polymer. The tablets were formulated at three different ratios with the polymer to investigate the effect of different concentrations of polymer on in vitro drug release patterns/kinetics and in vivo absorption/pharmacokinetics. Pre-formulation studies were conducted including bulk density, tapped density, compressibility index, Hausner ratio and angle of repose. In vitro studies were conducted using phosphate buffer (pH 7.4) as dissolution medium. In vivo performance was evaluated using albino rabbits. Physico-chemical characteristics (i.e. dimensional tests, weight variation, hardness, friability and drug content determination) fell in the USP acceptable limits. The compressibility index was found to range between 12.02±0.01% and 18.66±0.03%, the Hausner ratio varied between 1.02±0.01 and 1.19±0.10 and the angle of repose ranged from 15.19±0.01 to 24.52±0.10, all indicating better flow properties than the bulk-reference standard. Both bulk and tapped densities also fell in the USP acceptable range. Ibuprofen market tablets showed Tmax of 2.1±0.4h, which was significantly (P-value <0.05) lower compared to that of the reference standard (i.e. 4.09±1.3h). Ibuprofen test formulation has a half-life (t1/2) of 16.9±2.5h, which was significantly (P-value<0.001) higher compared to that of the reference standard (i.e. 9.23±2.9h). Eudragit® S 100 polymers can be used efficiently to develop directly compressed prolonged release tablets.

Djeribi R.,Annaba University | Bouchloukh W.,Annaba University | Jouenne T.,French National Center for Scientific Research | Menaa B.,Annaba University | Menaa B.,Fluorotronics Inc.
American Journal of Infection Control | Year: 2012

Background: The formation of bacterial biofilms on urinary catheters is a leading cause of urinary tract infections in intensive care units. Cytobacteriological examination of urine from patients is often misleading, due to the formation of these biofilms. Therefore, characterizing these biofilms and identifying the bacterial species residing on the surface of catheters are of major importance. Methods: We studied the formation of biofilms on the inner surface of urinary catheters using microbiological culture techniques, with the direct contact of catheter pieces with blood agar. The bacterial species on the surface were characterized by scanning electron microscopy, and the kinetic profile of biofilm formation on a silicone substrate for an imipenem-resistant Acinetobacter baumannii bacterium was evaluated with a crystal violet staining assay. Results: The bacterial species that constituted these biofilms were identified as a variety of gram-negative bacilli, with a predominance of strains belonging to Pseudomonas aeruginosa. The other isolated strains belonged to A baumannii and Klebsiella ornithinolytica. Kinetic profiling of biofilm formation identified the transient behavior of A baumannii between its biofilm and planktonic state. This strain was highly resistant to all of the antibiotics tested except colistin. Scanning electron microscopy images showed that the identified isolated species formed a dense and interconnected network of cellular multilayers formed from either a single cell or from different species that were surrounded and enveloped by a protective matrix. Conclusions: Microbiological analysis of the intraluminal surface of the catheter is required for true identification of the causative agents of catheter-associated urinary tract infections. This approach, combined with a routine cytobacteriological examination of urine, allows for the complete characterization of biofilm-associated species, and also may help prevent biofilm formation in such devices and help guide optimum antibiotic treatment. Copyright © 2012 by the Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

Djeribi R.,Annaba University | Boucherit Z.,Abou Bekr Belkaid University Tlemcen | Bouchloukh W.,Annaba University | Zouaoui W.,Annaba University | And 4 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2013

The first step in the biofilm formation is the bacterial attachment to solid surfaces, which is dependent on the bacteria cell surface physico-chemical properties. The purpose of this work was to analyze the effect of pH on the physicochemical cell surface properties of Acinetobacter baumannii by two different methods. The cell surface properties were evaluated using the microbial adhesion to solvents method (MATS) and contact angle measurements (CAM). MATS technique allowed us to enlighten that A. baumannii was hydrophilic at the different values of pH. It was found that at a desired pH of 6.5, the strain presents a maximum and stable value of electron-donor characteristic, while the electron acceptor character increased as the pH increased. Regardless of the methods employed, the obtained results using MATS and CAM confirmed the influence of the pH on the surface physicochemical properties of A. baumannii. The cell surface electron-donor and electron-acceptor character at pH 6.5 was found to be quite similar using both methods. © 2012 Elsevier B.V.

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