Fluoptics

Grenoble, France

Fluoptics

Grenoble, France
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Dorval P.,CNRS Computer Science and Engineering Laboratory | Mangeret N.,Fluoptics | Guillermet S.,Fluoptics | Righini C.A.,Joseph Fourier University | And 3 more authors.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2014

Near-infrared fluorescence image-guided surgery, FIGS, has lately shown a huge potential in oncologic and lymphatic related surgeries. In some indications such as liver or heart surgery, fluorescence-reachable anatomic structures are limited by the access to the surgical field. Nevertheless, most of the systems available on the market are too large to image the sides of cavities. Small devices are clearly required to improve workability of fluorescence imaging systems. The current work describes the development of an instrument and the results of its evaluation. In order to image narrow area, we developed a small size device consisting of an optical head connected to a control box. The whole system, optical head, control box and software, receives a CE mark for clinical procedures. Building on existing technologies, we simplified the fluorescence imaging system. It consists of a custom charged-coupled device camera, a high color rendering index visible LED illumination and a Class1 Laser fluorophore excitation. With a curved shape of 25x35x150mm, the optical head was designed as a true hand-held probe. The field of view varies from 5x3.75cm to 2x1.5cm. The device is able to collect and display the signal of 5pmol of IndoCyanine Green (ICG) with a spatial resolution down to 70μm at 25 frames per second. The system has been evaluated in pre-clinical and clinical procedures. The preclinical studies confirmed the ability of the system to visualize tumors in mice models. Clinical evaluations includes lymphedema investigations and surgical resections of tumors in colorectal cancer.


Rizo P.,Fluoptics | Dinten J.-M.,CEA Grenoble | Texier I.,CEA Grenoble
Bio Tribune Magazine | Year: 2010

Fluorescence probes and imaging methods have been extensively developed in microscopy to visualize biological pathways, cell trafficking and intracellular interactions, which are the main targets of molecular imaging. The translation of these methods from microscopy to preclinical and clinical applications requires to image through large thickness of live biological tissues, and to ensure the non-toxicity of the probes. We hereafter list the main issues that must be addressed to translate fluorescence techniques to clinic, and we present the main envisioned solutions. As first realistic clinical application, we present work in progress on intraoperative fluorescence guided surgery. © 2009 Springer Verlag France.


Antakia R.,University of Sheffield | Gayet P.,Fluoptics | Stephanie G.,Fluoptics | Stephenson T.J.,Sheffield Teaching Hospitals | And 3 more authors.
Journal of Surgical Research | Year: 2014

Background: Near infrared fluorescence imaging using intravenous methylene blue (MB) is a novel technique that has potential to aid the parathyroid gland (PG) localization during thyroid and parathyroid surgery. The aim of this study was to examine MB fluorescence in the rabbit neck and determine the influence of MB dose and time following administration on fluorescence from thyroid and PGs. Methods: Thyroid and external PGs were exposed in six New Zealand white rabbits under anesthesia. Varying doses of MB (0.025-3 mg/kg) were injected through the marginal ear vein. Near infrared fluorescence from exposed tissues was recorded at different time intervals (10-74 min) using Fluobeam 700. Specimens of identified glands were then resected for histologic assessment. Results: Histology confirmed accurate identification of all excised thyroid and PGs; these were the only neck structures to demonstrate significant fluorescence. The parathyroid demonstrated lower fluorescence intensities and reduced washout times at all MB doses compared with the thyroid gland. A dose of 0.1 mg/kg MB was adequate to identify fluorescence; this also delineated the blood supply of the external PGs. Conclusions: The study demonstrates that near infrared fluorescence with intravenous MB helps differentiate between thyroid and PGs in the rabbit. This has potential to improve outcomes in thyroid and parathyroid surgery by increasing the accuracy of parathyroid identification; however, the findings require replication in human surgery. The use of low doses of MB may also avoid the side effects associated with currently used doses in humans (3-7 mg/kg). © 2014 Elsevier Inc. All rights reserved.


Dutour A.,Center Leon Berard | Dutour A.,French Institute of Health and Medical Research | Josserand V.,French Institute of Health and Medical Research | Josserand V.,Joseph Fourier University | And 12 more authors.
Bone | Year: 2014

Tumor size and location along with efficacy of pre-operative imaging are limiting factors for optimal surgical excision for osteosarcoma. Our general hypothesis is that targeting αvβ3 integrin-rich osteosarcoma neoangiogenesis should provide improved delivery of diagnostic compounds and assist surgeons intra operatively using near-infrared imaging techniques. We evaluated in an orthotopic metastatic osteosarcoma in rats the potential of AngioStamp™ targeting αvβ3 integrins and detected intra operatively by near infrared (NIR) illumination (Fluobeam™) as a novel, intra operative imaging technique. To determine the potential of this association in improving tumor and metastasis detection, we compared the quality and sensitivity of tumor/metastasis margin delineation and tumor resection using intra-operative NIR imaging to the ones guided by pre-operative imaging (i.e., MRI subsequently confirmed by histopathological analysis). Chemotherapy being essential in osteosarcoma treatment, we evaluated the capacity of AngioStamp™ to specifically localize to the tumor after chemotherapy treatment. We showed a significantly lesser extent of healthy tissue resection after surgical excision when assessing tumor margin intra operatively using AngioStamp™/Fluobeam™ association compared to pre-operative MRI post-operatively confirmed by histopathological analysis (p<0.01). Importantly, intra-operative NIR illumination of lungs revealed more metastases than were detected by CT Scan or under intra-operative white light examination (p<0.01). Importantly, chemotherapy did not alter AngioStamp™ tumor specific targeting nor the sensitivity of tumor detection. Our preclinical data confirm the potential of intra-operative imaging for improved primary tumor and lung metastasis excision. Based on these promising results, we now propose to evaluate this approach as a mean to improve surgical excision while maintaining tumor control in other sarcoma or tumors overexpressing αvβ3 integrins. © 2014 Elsevier Inc.


Mery E.,Institute Claudius Regaud | Mery E.,University Paul Sabatier | Jouve E.,Institute Claudius Regaud | Jouve E.,University Paul Sabatier | And 14 more authors.
Gynecologic Oncology | Year: 2011

Objective: Improvement of the management and outcome of ovarian cancers may require intraoperative detection and therapeutic intervention to treat minimal residual disease after complete surgery. The aim of this study was to validate the importance of fluorescence in the peroperative detection of human ovarian adenocarcinoma cells and to determine its efficiency in detecting infra millimetric tumor metastases. Methods: A fluorescent RAFT-(cRGD)4 tracer molecule (AngioStamp®) was used. The tracer is based on a biomarker, which has a very high affinity for the αvβ3 integrin, which is overexpressed in a large ratio of cancer cells and neovessel endothelial cells during angiogenesis. Infrared fluorescence was visualized with Fluobeam®, an open fluorescent imaging system that could potentially be used in peroperative conditions in the future. Results: This novel technique allowed the specific detection of residual tumor deposits and inframillimetric metastases, smaller than 500 μm, which were resected under fluorescent guidance. AngioStamp® was able to detect all types of cell lines, derived from human ovarian adenocarcinomas, before or after chemotherapy treatment in animals. The effectiveness of AngioStamp® for the detection of various human ovarian adenocarcinomas was assessed on 10 different fragments of tumor, implanted subcutaneously in nude mice. All implanted tumor fragments were visualized by AngioStamp®. Conclusions: The high rate of recurrence after apparently complete surgery and/or complete clinical response to chemotherapy implies that most patients have undetected minimal residual disease. Novel techniques such as laparoscopic or laparotomic fluorescence may prove to be crucial in reassessing the definition of primary outcome in ovarian cancer management. © 2011 Elsevier Inc. All rights reserved.


Wenk C.H.F.,French Institute of Health and Medical Research | Wenk C.H.F.,Joseph Fourier University | Ponce F.,VetAgro Sup | Guillermet S.,Fluoptics | And 10 more authors.
Cancer Letters | Year: 2013

We investigated how near-infrared imaging could improve highly infiltrative spontaneous fibrosarcoma surgery in 12 cats in a clinical veterinary phase. We used an RGD-based nanoprobe at different doses and times before surgery and a portable clinical grade imaging system. All tumours were labelled by the tracer and had an overall tumour-to-healthy tissue ratio of 14. ±. 1 during surgery. No false negatives were found, and the percentage of tumour cells was linearly correlated with the fluorescence intensity. All cats recovered well and were submitted to long-term follow-up that is currently on-going 1. year after the beginning of the study. © 2012 Elsevier Ireland Ltd.


Patent
Fluoptics | Date: 2013-04-23

Fluorescence imaging system for an operating theatre, comprising a device illuminating the operating theatre and emitting a white light, and a fluorescence imaging device. Said fluorescence imaging device comprises a light source emitting radiation for excitation of a fluorescent marker in a range of emission wavelengths of between 600 and 900 nm. The light emitted by the illuminating device is filtered by a low-pass filter, of which the cut-off wavelength is below the emission range of the fluorescent marker, but is nonetheless able to show an increase or fluctuations in the attenuation for wavelengths above the emission range of the fluorescent marker, the product of the attenuation of the filter of the detector and of the attenuation of the low-pass filter of the illuminating device leading to an attenuation by a factor of at least 10^(6).


Trademark
Fluoptics | Date: 2012-10-23

Fluorescence imaging apparatus for medical use in surgery in the medical and veterinary fields and in the fields of pharmaceutical and biological research, including a source of illumination in white light, a fluorescent marker excitation source and a device for detecting fluorescence.


PubMed | University of Sheffield, Sheffield Teaching Hospitals and Fluoptics
Type: Journal Article | Journal: The Journal of surgical research | Year: 2014

Near infrared fluorescence imaging using intravenous methylene blue (MB) is a novel technique that has potential to aid the parathyroid gland (PG) localization during thyroid and parathyroid surgery. The aim of this study was to examine MB fluorescence in the rabbit neck and determine the influence of MB dose and time following administration on fluorescence from thyroid and PGs.Thyroid and external PGs were exposed in six New Zealand white rabbits under anesthesia. Varying doses of MB (0.025-3mg/kg) were injected through the marginal ear vein. Near infrared fluorescence from exposed tissues was recorded at different time intervals (10-74min) using Fluobeam 700. Specimens of identified glands were then resected for histologic assessment.Histology confirmed accurate identification of all excised thyroid and PGs; these were the only neck structures to demonstrate significant fluorescence. The parathyroid demonstrated lower fluorescence intensities and reduced washout times at all MB doses compared with the thyroid gland. A dose of 0.1mg/kg MB was adequate to identify fluorescence; this also delineated the blood supply of the external PGs.The study demonstrates that near infrared fluorescence with intravenous MB helps differentiate between thyroid and PGs in the rabbit. This has potential to improve outcomes in thyroid and parathyroid surgery by increasing the accuracy of parathyroid identification; however, the findings require replication in human surgery. The use of low doses of MB may also avoid the side effects associated with currently used doses in humans (3-7mg/kg).


PubMed | Fluoptics, University Claude Bernard Lyon 1, French Institute of Health and Medical Research, Center Leon Berard and Joseph Fourier University
Type: | Journal: Bone | Year: 2014

Tumor size and location along with efficacy of pre-operative imaging are limiting factors for optimal surgical excision for osteosarcoma. Our general hypothesis is that targeting v3 integrin-rich osteosarcoma neoangiogenesis should provide improved delivery of diagnostic compounds and assist surgeons intra operatively using near-infrared imaging techniques. We evaluated in an orthotopic metastatic osteosarcoma in rats the potential of AngioStamp targeting v3 integrins and detected intra operatively by near infrared (NIR) illumination (Fluobeam) as a novel, intra operative imaging technique. To determine the potential of this association in improving tumor and metastasis detection, we compared the quality and sensitivity of tumor/metastasis margin delineation and tumor resection using intra-operative NIR imaging to the ones guided by pre-operative imaging (i.e., MRI subsequently confirmed by histopathological analysis). Chemotherapy being essential in osteosarcoma treatment, we evaluated the capacity of AngioStamp to specifically localize to the tumor after chemotherapy treatment. We showed a significantly lesser extent of healthy tissue resection after surgical excision when assessing tumor margin intra operatively using AngioStamp/Fluobeam association compared to pre-operative MRI post-operatively confirmed by histopathological analysis (p<0.01). Importantly, intra-operative NIR illumination of lungs revealed more metastases than were detected by CT Scan or under intra-operative white light examination (p<0.01). Importantly, chemotherapy did not alter AngioStamp tumor specific targeting nor the sensitivity of tumor detection. Our preclinical data confirm the potential of intra-operative imaging for improved primary tumor and lung metastasis excision. Based on these promising results, we now propose to evaluate this approach as a mean to improve surgical excision while maintaining tumor control in other sarcoma or tumors overexpressing v3 integrins.

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