Flower Research Institute

Kunming, China

Flower Research Institute

Kunming, China
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Li J.,Yunnan Academy of Agricultural Sciences | Lu L.,Flower Research Institute | Jia Y.,U.S. Department of Agriculture | Wang Q.,Agricultural Environment and Resources Research Institute | And 2 more authors.
Plant Disease | Year: 2016

Due to the harmful nature of the rice blast fungus, Magnaporthe oryzae, it is beneficial to characterize field isolates to help aid in the deployment of resistance (R) genes in rice. In the present study, 252 field isolates of M. oryzae, collected from rice fields of Yunnan Province in China, were assessed for mating type, DNA fingerprinting, and disease reactions to differential rice lines. In total, 94 isolates (37.3%) were MAT1-1 and 158 (62.7%) were MAT1-2 based on polymerase chain reaction assays, and some of them were verified with the tester isolates. All MAT1-1 and MAT1-2 isolates were virulent to some of the International Rice Research Institute–Japan International Research Center for Agricultural Sciences monogenic lines harboring 22 major resistance genes as differential varieties. Three simple-sequence repeat markers were used to examine genetic diversity in all isolates. The existence of regional patterns of genetic diversity, sexual reproduction potential, and pathogenicity suggests that M. oryzae populations have been independently asexually adapted in rice fields during crop cultivation. © 2016 The American Phytopathological Society.

Chung M.-Y.,Sunchon National University | Kim M.B.,Sunchon National University | Chung Y.M.,Flower Research Institute | Nou S.,Sunchon National University | Kim C.K.,Kyungpook National University
Journal of Plant Biotechnology | Year: 2016

This research was conducted to improve the cold tolerance of the gerbera cv. Gold Eye by introduction of the Arabidopsis Ca 2+ /H + antiporter gene (CAX1) via Agrobacteriummediated transformation. Prior to genetic transformation, we optimized a combination of plant growth regulators; 1.0 mgl-1 6-Benzyladenine (BA) and 0.1 mgl-1 3-indole-Acetic acid (IAA) were found to lead to proper in vitro shoot regeneration from petiole explants. In addition, 50 mgl-1 kanamycin was determined to be the minimal concentration useful for selection of putative transgenic plants. In this study, transgenic gerbera expressing the Arabidopsis Ca 2+ /H + antiporter gene (CAX1) were obtained using the optimized concentrations. We expect that introduction of the gene to the cultivar will improve cold tolerance, which will be important in the winter months. © Korean Society for Plant Biotechnology.

Duan C.F.,Tropical and Subtropical Cash Crops Research Institute | Long Y.Q.,Tropical and Subtropical Cash Crops Research Institute | Chen H.,Yunnan Academy of Agricultural Sciences | Yang G.H.,Yunnan Agricultural University | And 2 more authors.
EPPO Bulletin | Year: 2015

A severe disease of carnation flowers was observed in Kunming, China in November 2009. Initial symptoms on flowers and immature flower buds were characterized by water-soaked areas. Spots with purple margins could be seen on petals of some varieties. An Alternaria sp. was isolated from 100% of symptomatic tissues sampled. The isolates showed high virulence when inoculated onto tissue culture plantlets and on healthy carnation flowers. Results of sequence analysis of 5.8S rDNA-ITS of isolates showed 99% sequence similarity with Alternaria dianthicola in GenBank. To the best of the authors' knowledge, this was the first sighting of blight of flowers and immature flower buds of carnation caused by Alternaria dianthicola in China. © 2015 The Authors.

Chi Y.H.,Gyeongsang National University | Kim S.Y.,Flower Research Institute | Lee E.S.,Gyeongsang National University | Jung Y.J.,National Institute of Ecology | And 6 more authors.
Biochemical and Biophysical Research Communications | Year: 2016

To identify novel roles of SMALL RUBBER PARTICLE PROTEIN Homolog in the non-rubber-producing plant Arabidopsis (AtSRP1), we isolated a T-DNA-insertion knock-out mutant (FLAG-543A05) and investigated its functional characteristics. AtSRP1 is predominantly expressed in reproductive organs and is localized to lipid droplets and ER. Compared to wild-type (WT) Arabidopsis, atsrp1 plants contain small siliques with a reduced number of heterogeneously shaped seeds. The size of anther and pollen grains in atsrp1 is highly irregular, with a lower grain number than WT. Therefore, AtSRP1 plays a novel role related to pollen growth and development in a non-rubber-producing plant. © 2016 Elsevier Inc. All rights reserved.

Park Y.H.,Pusan National University | Ahn S.G.,Pusan National University | Choi Y.M.,Pusan National University | Oh H.J.,Pusan National University | And 5 more authors.
Scientia Horticulturae | Year: 2010

The 'Genome database for Rosaceae (GDR)' provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei-Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 ('Grand King' and 'Carnival') to 0.99 ('First Red' and 'Red Champ'). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 ('Maehyang' and 'Janghyee') to 0.64 ('Janghyee' and 'Pragana'). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid 'Pragana' was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae. © 2010 Elsevier B.V.

Jeon Y.,Chungnam National University | Jin Y.-T.,Chungnam National University | Choi S.-H.,Chungnam National University | Park N.,Chungnam National University | And 4 more authors.
Journal of Plant Biotechnology | Year: 2016

This study was conducted to investigate the potential use of New Zealand spinach (Tetragonia tetragonioides) as a new vegetable crop which will be cultivated in salt-Affected soils such as reclaimed areas. New Zealand spinach ecotypes native to Korea were collected across the Southern, Western and Eastern seashore regions of the Korean peninsula, among which fifty-five accessions were later further propagated and evaluated genetically by using an AFLP (amplified fragment length polymorphism) marker. Based on the AFLP analysis performed to uncover the genetic diversity of the collected ecotypes, enzymatic cleavage of the extracted DNA was implemented based on 12 EcoRI and MseI combinations. A total of 1,279 alleles (107 alleles per EcoRI and MseI enzyme combination) were successfully amplified, among which 62 alleles per enzyme combination were polymorphic (58%). The AFLP analysis indicated that the rate of genetic dissimilarity was 29% among the New Zealand spinach collections, which were clustered into the 7 genetic diversity group. This is the first report on the genetic variation in the genus Tetragonia, and the basic information can be applied to select parental lines for enhancing the segregation spectrum of the new halophytic vegetable plant grown in salt-Affected areas. © Korean Society for Plant Biotechnology.

Hwang J.-C.,Flower Research Institute | Chin Y.-D.,Flower Research Institute | Chung Y.-M.,Flower Research Institute | Kim S.-K.,Flower Research Institute | And 2 more authors.
Korean Journal of Horticultural Science and Technology | Year: 2013

A new spray chrysanthemum (Dendranthema grandiflorum) 'Blue Hope' was bred by the Flower Research Institute, Gyeongsangnam-do Agricultural Research & Extension Services in 2009. The cultivar 'Blue Hope' was initially derived from the cross in 2005 between 'Ford', a spray chrysanthemum cultivar, with white anemone type, and 'Chopin', a spray chrysanthemum cultivar with white anemone type. The cultivar has anemone type with white petals. After the evaluation of the characteristics under shade culture in summer and retarding culture in spring and consecutive selection from 2007 to 2009, 'Blue Hope' was selected finality. The natural flowering time of 'Blue Hope' was October 24th, and year-round flowering is possible by shading or lighting treatment. The growth of plant was very vigorous and response time 6.5 weeks. The diameter of flower was 4.9 cm. Number of flowers per stem was 19.1 in autumn. Days to flowering under the short day treatment was about 45 in spring and its vase life was 23.8 days in the autumn season. This cultivar was resistance white rust and consumer's preference of new spray is high level than the control.

Chung Y.M.,Flower Research Institute | Hwang J.C.,Flower Research Institute | Chin Y.D.,Flower Research Institute | Lee J.S.,South Korean National Institute of Animal Science | And 4 more authors.
Korean Journal of Horticultural Science and Technology | Year: 2013

A new pink gerbera (Gerbera hybrida Hort.) cultivar 'Party Time' was developed from a cross between 'Hope' and 'Sunnyeo', followed by seedling and line selections at the Flower Research Institute of Gyeongnam Agricultural Research and Extension Services (GARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2005 to 2007. 'Party Time' has semi-double type large flowers in a good harmony with pink (RHS, 65-C) ray floret and brown center. It has a good, stable flower shape and strong peduncles, and its vase life was 12.7 days. The average flower yield of 'Party Time' was about 47.3 stems per plant a year in greenhouse yield trails carried out 2005 to 2007. 'Party Time' (Grant No. 2691) was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009.

Kim D.G.,Flower Research Institute | Kim K.K.,Flower Research Institute | Been C.G.,Flower Research Institute
Horticulture Environment and Biotechnology | Year: 2015

To develop a new type of orchid with low-temperature tolerance and good fragrance, we generated intergeneric crosses between Korea-native wind orchids and tropical Phalaenopsis. In 2009, 160 cross combinations were made among Sedirea japonica, Phalaenopsis, Doritaenopsis and Neofinetia falcata. High incompatibility was observed from most of crosses as evidenced by traits such as early dropping of seed pods after pod formation or no seed formation after pod maturation. A total of 528 hybrid seedlings were obtained from 12 cross combinations. From these hybrid plants, several lines were selected based on flower type, inflorescence, cold tolerance, and fragrance, and finally the three best lines were selected. The selected lines were generally intermediate in both phenotype and genetic characters of the two parents, but tended to be more similar to Phalaenopsis. © 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH.

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