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Li G.,City of Hope National Medical Center | Warden C.,City of Hope National Medical Center | Zou Z.,City of Hope National Medical Center | Neman J.,City of Hope National Medical Center | And 4 more authors.
PLoS ONE | Year: 2013

The Polycomb group (PcG) proteins play a critical role in histone mediated epigenetics which has been implicated in the malignant evolution of glioblastoma multiforme (GBM). By systematically interrogating The Cancer Genome Atlas (TCGA), we discovered widespread aberrant expression of the PcG members in GBM samples compared to normal brain. The most striking differences were upregulation of EZH2, PHF19, CBX8 and PHC2 and downregulation of CBX7, CBX6, EZH1 and RYBP. Interestingly, changes in EZH2, PHF19, CBX7, CBX6 and EZH1 occurred progressively as astrocytoma grade increased. We validated the aberrant expression of CBX6, CBX7, CBX8 and EZH2 in GBM cell lines by Western blotting and qRT-PCR, and further the aberrant expression of CBX6 in GBM tissue samples by immunohistochemical staining. To determine if there was functional significance to the diminished CBX6 levels in GBM, CBX6 was overexpressed in GBM cells resulting in decreased proliferative capacity. In conclusion, aberrant expression of PcG proteins in GBMs may play a role in the development or maintenance of the malignancy. © 2013 Li et al.

Lange H.,Flagship Biosciences
Laboratory Medicine | Year: 2011

Digital pathology is a new technology and industry. Official agencies, including the Clinical Laboratory Improvement Amendments (CLIA), the College of American Pathologists (CAP), and the U.S. Food and Drug Administration (FDA), provide little guidance, and manufacturers still have to learn what it means to provide instruments to a clinical laboratory.With digital pathology now entering clinical laboratories, it is crucial for physicians and laboratory professionals to understand the regulatory requirements and how to best implement them in their clinical laboratories. The goal of this article is to provide those professionals with a comprehensive regulatory overview and a reference framework for their future work in digital pathology. This article presents the different regulatory requirements separately-first for clinical laboratories and then for medical device manufacturers.

Suraneni M.V.,University of Texas | Suraneni M.V.,Flagship Biosciences | Moore J.R.,University of Texas | Zhang D.,University of Texas | And 6 more authors.
Cell Cycle | Year: 2014

15-Lipoxygenase-2 (15-LOX2) is a human-specific lipid-peroxidizing enzyme most prominently expressed in epithelial cells of normal human prostate but downregulated or completely lost in > 70% of prostate cancer (PCa) cases. Transgenic expression of 15-LOX2 in the mouse prostate surprisingly causes hyperplasia. Here we first provide evidence that 15-LOX2-induced prostatic hyperplasia does not progress to PCa even in p53+/- or p53 -/- background. More important, by generating 15-LOX2; Hi-Myc double transgenic (dTg) mice, we show that 15-LOX2 expression inhibits Myc-induced PCa development, such that in the 3-month- and 6-month-old dTg mice, there is a significant reduction in prostate intraneoplasia (PIN) and PCa prevalent in age-matched Hi-Myc prostates. The dTg prostates show increased cell senescence and expression of several senescence-associated molecules, including p27, phosphorylated Rb, and Rb1cc1. We further show that in HPCa, 15-LOX2 and c-Myc manifest reciprocal protein expression patterns. Moreover, RB1CC1 accumulates in senescing normal human prostate (NHP) cells, and in both NHP and RWPE-1 cells, the 15-LOX2 metabolic products 15(S)-HPETE and 15(S)-HETE induce RB1CC1. We finally show that unlike 15-LOX2, RB1CC1 is not lost but rather frequently overexpressed in PCa samples. RB1CC1 knockdown in PC3 cells enhances clonal growth in vitro and tumor growth in vivo. Together, our present studies provide evidence for tumor-suppressive functions for both 15-LOX2 and RB1CC1. © 2014 Landes Bioscience.

Richardson F.,OSI Pharmaceuticals Inc. | Young G.D.,Flagship Biosciences | Sennello R.,OSI Pharmaceuticals Inc. | Wolf J.,Novella Clinical | And 5 more authors.
Anticancer Research | Year: 2012

E-Cadherin and vimentin protein expression was assessed in late stage non-small cell lung cancer tumors from the placebo controlled clinical trial, NCIC-CTG BR.21, to determine if these markers had the potential to predict outcome of erlotinib therapy. E-Cadherin and vimentin protein expression levels were assessed in tumors from 95 patients, who were representative of the overall population, using semi-quantitative immunohistochemistry. The percentage of tumor cells with grades 0, 1, 2, or 3 membrane staining of E-cadherin and cytoplasmic staining of vimentin was measured. Three scoring methods and multiple cut-offs were explored to determine if these markers were able to divide patients into groups with different overall survival (OS). A cut-off point for E-cadherin of ≥40% tumor cells with staining of +2 and +3 and a cut-off for vimentin of ≥10% of tumors cell with any staining provided the optimal stratification. The OS hazard ratio (HR) for E-cadherin+ versus E-cadherir - in the erlotinib-treated patients was 0.68 (0.35-1.33) compared with 1.48 (0.69-3.15) in the placebo patients and the OS (HR) for erlotinib versus placebo was 0.47 (0.26-0.88) in E-cadherin + patients compared with 1.12 (0.52-2.44) in the E-cadherin - patients. The OS (HR) for vimentin + versus vimentin - in the erlotinib-treated patients was 0.65 (0.31-1.38) compared to 2.32 (1.09-4.94) in the placebo-treated patients and the OS (HR) for erlotinib versus placebo was 0.26 (0.11-0.63) in vimentin + compared to 0.99 (0.55-1.76) in the vimentin - patients. Similar trends were observed for progression-free survival and response rate. E-Cadherin and vimentin are biomarker s worthy of additional study as predictive markers of outcome of erlotinib therapy.

Aeffner F.,Flagship Biosciences | Bolon B.,Ohio State University | Bolon B.,GEMpath Inc. | Davis I.C.,GEMpath Inc.
Toxicologic Pathology | Year: 2015

Acute respiratory distress syndrome (ARDS) is a severe pulmonary reaction requiring hospitalization, which is incited by many causes, including bacterial and viral pneumonia as well as near drowning, aspiration of gastric contents, pancreatitis, intravenous drug use, and abdominal trauma. In humans, ARDS is very well defined by a list of clinical parameters. However, until recently no consensus was available regarding the criteria of ARDS that should be evident in an experimental animal model. This lack was rectified by a 2011 workshop report by the American Thoracic Society, which defined the main features proposed to delineate the presence of ARDS in laboratory animals. These should include histological changes in parenchymal tissue, altered integrity of the alveolar capillary barrier, inflammation, and abnormal pulmonary function. Murine ARDS models typically are defined by such features as pulmonary edema and leukocyte infiltration in cytological preparations of bronchoalveolar lavage fluid and/or lung sections. Common pathophysiological indicators of ARDS in mice include impaired pulmonary gas exchange and histological evidence of inflammatory infiltrates into the lung. Thus, morphological endpoints remain a vital component of data sets assembled from animal ARDS models. © 2015 by The Author(s).

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