Fisiologia i Immunologia UAB

Spain

Fisiologia i Immunologia UAB

Spain
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Ibarlucea B.,CSIC - National Center of Microelectronics | Vila-Planas J.,CSIC - National Center of Microelectronics | Fernandez-Rosas E.,CSIC - National Center of Microelectronics | Demming S.,TU Braunschweig | And 4 more authors.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 | Year: 2010

A low-cost photonic lab on a chip with three different working regimes for cell screening is presented, namely scattering, scattering+absorbance and absorbance. In all regimes, the whole spectral response in absorbance is obtained, being possible to determine for each regime which is the optimal working wavelength that provides the highest performance. Experimental limits of detection (LOD) of 54.9 ± 0.7 cells, 53 ± 1 cells, and 105 ± 4 cells, depending on the working regime used, have been obtained. The system has also been used for measuring the dead/live cell ratio, obtaining LODs between 7.6 ± 0.4% and 6.7 ± 0.3% of dead cells.


Ortiz P.,CSIC - National Center of Microelectronics | Demming S.,Fisiologia i Immunologia UAB | Fernandez-Rosas E.,TU Braunschweig | Ibarlucea B.,CSIC - National Center of Microelectronics | And 3 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2011

In this paper two different photonic lab-on-a-chip devices for real-time cell screening are presented. We discuss, in parallel, screening of cell viability and cell count using a multiple total internal reflection (MIR) system, and a microbioreactor (MBR) for continuous cell culture screening. Each device takes advantage, in different ways, of the enhanced light-analyte interaction provided by the full-field approach. Their performance and how they represent an improvement to current methods, are also discussed herein. Both devices integrate photonic components and are fabricated by soft lithography (PDMS) replica molding of SU-8 masters, thus making them low-cost and easily mass produced. © 2011 SPIE.

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