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Yoon T.-H.,Pukyong National University | Bae J.,Pukyong National University | Kang H.-E.,Pukyong National University | Choi J.H.,Fisheries Resources Research Division | And 3 more authors.
Ocean Science Journal | Year: 2015

The molecular markers to distinguish different larval stages have various applications in ecological studies. Using the differential display RT-PCR technique, we isolated and characterized four genes, which are expressed predominantly in the megalopa stage of Eriocheir sinensis. The four genes include two cuticular proteins with different domain organization (Ers-CP15 and Ers-CP34) and two skeletal-muscle-specific genes (Ers-SCP and Ers-ActinSK1). The two cuticular protein genes were expressed predominantly in the epidermis and their expression level was significantly higher in the megalopa stage (about 7.0-folds) than it was in the zoea stage. However, their high transcriptional level in zoea IV suggested that these two cuticular protein genes may not be a useful target to discriminate megalopa from zoea. Ers-SCP encoded the invertebratespecific sarcoplasmic calcium binding protein and Ers-ActinSK1 gene encoded the crustacean skeletal muscle actin. Expressions of these two genes were detected only in muscular tissues; leg muscle, claw muscle and thoracic muscle. This suggests that the increased transcription levels of two muscle-specific genes during the megalopa stage are mainly due to increased muscular tissues. Among its three isoforms, Ers-SCPa displayed the highest difference (22.4-folds) between megalopa and zoea suggesting Ers-SCPa is the most reliable marker to distinguish megalopa from zoea. Although Ers-SCPc and Ers-ActinSK1 also showed similar expression profiles to Ers-SCPa and Ers-SCPb, differences in their expression levels were not as high as Ers-SCPa and Ers-SCPb. © 2015, Korea Ocean Research & Development Institute (KORDI) and the Korean Society of Oceanography (KSO) and Springer Science+Business Media Dordrecht. Source

Cho E.-S.,South Korean National Fisheries Research and Development Institute | Seo Y.-I.,Fisheries Resources Research Division | Suh Y.-S.,South Korean National Fisheries Research and Development Institute
Journal of Environmental Biology | Year: 2013

To investigate the genetic structure of the purplish Washington clam population, Saxidomus purpuratus Sowerby, in Korea. A portion of mitochondrial COI gene sequences (605 bp) for phylogenetic comparison was determined. Sequence analysis of 62 individuals collected from six regions revealed 13 haplotypes. Phylogenetic analysis using Phylogeny Inference Package (PHYLIP) subdivided the purplish Washington clam into two clades {termed clade A and B), weak supported groups (<65 of bootstrap value). This haplotype subdivision was also in accordance with geographic separation; one each at Masan, Yeosu, Samcheonpo, Jubyeon and Geojedo, and the other at Sineju. Population genetic analysis subdivided these two population groups with a geographic distance (c/=0.431, p=0.379). Furthermore, in the Sineju population, the maximum sequence divergence (2.67%) and minimum nucleotide diversity (0.0012426) were shown in which might be reflective of a relatively small population size and the geographical isolation of the population as compared with other populations. However, a vjery high migration rate (NM=59.62-infinite) and a very low level of geographic distance (FST=-0.076-0.055) were noted to exist among the South and East Sea populations, suggesting that individuals between populations should show a significantly active genetic mixing and migration regardless of geography. These findings allowed us to conclude that the purplish Washington clam populations occurring in the South and East Sea were formed with randomly dispersed individuals. © Triveni Enterprises, Lucknow (India). Source

Kang J.-H.,Biotechnology Research Division | Noh E.-S.,Biotechnology Research Division | Park J.-Y.,Biotechnology Research Division | An C.-M.,Biotechnology Research Division | And 4 more authors.
Asian-Australasian Journal of Animal Sciences | Year: 2015

Acetes chinensis is an economically important shrimp that belongs to the Sergestidae family; following fermentation, A. chinensis' economic value, however, is low in China, and much of the catch in China is exported to Korea at a low price, thus leading to potential false labeling. For this reason, we developed a simple method to identify A. chinensis' origin using allele-specific polymerase chain reaction (PCR). Ten single nucleotide polymorphisms (SNPs) were identified from partial (i.e., 570 bp) DNA sequence analysis of the mitochondrial 16s rRNA gene in 96 Korean and 96 Chinese individual shrimp. Among 10 SNP sites, four sites were observed in populations from both countries, and two sites located in the middle with SNP sites at their 3'-ends were used to design allele-specific primers. Among the eight internal primers, the C220F primer specific to the Chinese A. chinensis population amplified a DNA fragment of 364 bp only from that population. We were able to identify the A. chinensis population origin with 100% accuracy using multiplex PCR performed with two external primers and C220F primers. These results show that the 16S rRNA gene that is generally used for the identification of species can be used for the identification of the origin within species of A. chinensis, which is an important finding for the fair trade of the species between Korea and China. Copyright © 2015 by Asian-Australasian Journal of Animal Sciences Source

Setyobudi E.,Gangneung - Wonju National University | Setyobudi E.,Gadjah Mada University | Jeon C.-H.,Gangneung - Wonju National University | Choi K.,Fisheries Resources Research Division | And 3 more authors.
Ocean Science Journal | Year: 2013

The occurrence of Genus Anisakis nematode larvae in marine fishes and cephalopods is epidemiologically important because Anisakis simplex larval stage can cause a clinical disease in humans when infected hosts are consumed raw. Common squid (Todarodes pacificus) from Korean waters were investigated for anisakid nematodes infection during 2009∼2011. In total, 1,556 larvae were collected from 615 common squids and 732 of them were subsequently identified by PCR-RFLP analysis of ITS rDNA. Depending on the sampling locations, the nematode larvae from common squid showed different prevalence, intensity and species distribution. A high prevalence (P) and mean intensity (MI) of infection were observed in the Yellow Sea (n = 250, P = 86.0%, MI = 5.99 larvae/host) and the southern sea of Korea (n = 126, P = 57.1%, MI = 3.36 larvae/host). Anisakis pegreffii was dominantly found in common squid from the southern sea (127/ 140, 90.7%) and the Yellow Sea (561/565, 98.9%). In contrast, the P and MI of infection were relatively low in the East Sea (n = 239, P = 8.37%, MI = 1.25 larvae/host). A. pegreffii was not found from the East Sea and 52.0% (13/25) of the nematodes were identified as A. simplex. Most of them were found in the body cavity or digestive tract of common squid, which are rarely consumed raw by humans. Considering the differenences in anisakid nematode species distribution and their microhabitat in common squid, it remains unclear whether common squid plays an important role in the epidemiology of human anisakis infection in Korea. Further extensive identification of anisakid nematodes in common squid, with geographical and seasonal information will be necessary. © 2013 Korea Ocean Research & Development Institute (KORDI) and the Korean Society of Oceanography (KSO) and Springer Science+Business Media Dordrecht. Source

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