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Ma X.,Hunan University | Hu Y.,Hunan University | Wang X.-Q.,Hunan University | Ai Q.-H.,Ocean University of China | And 3 more authors.
Aquaculture International

A 10-week feeding experiment was conducted to evaluate the effects of dietary protein to lipid ratios (P/L) on growth, intestinal digestive enzyme activities and body composition in juvenile rice field eel (Monopterus albus) (initial mean body weight of 65.76 ± 1.07 g, mean ± SEM). Nine test diets were formulated in a 3 × 3 factorial design to contain three protein levels (350, 400 and 450 g kg-1) for each of three lipid levels (40, 80 and 120 g kg-1), respectively. Each diet was randomly assigned to triplicate groups of 60 fish per net cage (1.5 × 2.0 × 1.5 m). Results showed the survival was above 96 % and was not affected by dietary treatments. Fish fed the diet with 450 g kg-1 protein and 40 g kg-1 lipid showed the best weight gain (WG) (103.95 %) and feed conversion ratio (1.60) (P < 0.05). WG, protein efficiency ratio and energy retention increased with the increasing in lipid at 350 g kg-1 protein level (P < 0.05). However, WG showed a little decline with increasing dietary lipid when fish fed the diets with 400 and 450 g kg-1 protein level, but no significant difference was observed (P < 0.05). Hepatosomatic index, visceralsomatic index and intestinal lipase activity increased with the increasing of dietary lipid level irrespective protein level. Intestinal trypsin activity increased with the increasing of dietary lipid level when fish fed the diets with 350 g kg-1 protein, but showed converse trend when fish fed the diets with 400 and 450 g kg-1 protein. Serum triglyceride, body lipid and energy were positively correlated with the dietary lipid. Results of the present study showed that the dietary protein/lipid ratio of 450/40 g kg-1 is considered optimum for rice field eel under culture conditions, and the increase in dietary lipid level has no efficient protein-sparing effect when fish fed the diets with 400 and 450 g kg-1 protein level. © 2013 Springer Science+Business Media Dordrecht. Source

Liu L.,Hunan University | Liu L.,Fisheries Research Institute of Hunan Province | Li Y.L.,Hunan University | Xu S.D.,Guangdong VTR Bio Technology Co. | And 6 more authors.
Genetics and Molecular Research

Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including an open-reading frame of 1131 bp, which encoded 376 amino acids. Sequence analysis demonstrated that the MSTN shared a highly conserved signal peptide, a TGF-β functional peptide, a hydrolytic site (RARR), and nine conservative cysteine residues with other members of the TGF-β superfamily. Sequence alignment and phylogenetic tree analyses indicated that the MSTN had a close relationship with teleostean fish, but they are far separated from mammals. Real-time polymerase chain reaction analysis revealed that the MSTN was strongly expressed in the skeletal muscle and heart tissues. Temporal expression analysis demonstrated that the MSTN gene was expressed in very low levels, from 20 to 90 dph (post-hatching development), and was at its highest level at 150 dph (P < 0.05). The fasting-re-feeding experiment showed that the expression of the MSTN gene was initially decreased in response to a single meal, after seven days of fasting, and subsequently increased significantly, and finally decreased back to its original level. Together, our results provided valuable knowledge regarding the regulation of MSTN gene expression in Chinese perch. © FUNPEC-RP. Source

Liang Z.Q.,Southwest University | Liang Z.Q.,Chinese Academy of Fishery Sciences | Liang Z.Q.,Fisheries Research Institute of Hunan Province | Wang C.R.,Fisheries Research Institute of Hunan Province | And 7 more authors.
Journal of Applied Ichthyology

Length-weight relationships (LWR), W = aLb, were estimated for nine fish species belonging to three orders, four families and nine genera from the Hunan Zhangjiajie Chinese Giant Salamander National Natural Reserve, in the northwestern part of Hunan Province of central China. Six of the species are endemic to China, of which three are also endemic to the Yangtze River. The r2 value ranged from 0.9546 to 0.9924. Values of b varied from 2.9177 to 3.6752. This study represents the first reference on LWR for nine species, and are new maximum length records for six species. © 2015 Blackwell Verlag GmbH. Source

Hong B.,Fisheries Research Institute of Hunan Province | Wang D.-W.,Fisheries Research Institute of Hunan Province | Wu Y.-A.,Fisheries Research Institute of Hunan Province | Liu L.-L.,The Ministry of Agriculture Fishery Products Quality Supervision and Testing Center Changsha | And 3 more authors.
Modern Food Science and Technology

A method of microwave digestion-ICP/MS was established to determinate 8 kinds of trace element in mussel and bighead tissue. The sample was added with HNO3+H2O2 (3+1) after microwave digestion before ICP-MS determination. The introduction of Ge, In, Bi, Sc as internal standard substance can avoid spectral interferences, and the parameters of the apparatus was optimized. The detection limits of Fe, Cu, Zn, Mn, Cd, Pb, As and Hg were 2.3, 0.5, 1.1, 0.4, 0.6, 0.5, 1 and 1.8 μg/L, respectively, with the correlation coefficient higher than 0.9993. The recovery rate and relative standard deviation were 80.4~101.2% and less than 6.29%, respectively. The analysis of Lamprotula leai samples showed that Lamprotula leai was rich in Fe, Zn. However, several potentially harmful elements, such as Pb, Cd and Hg were also exist in the samples. Source

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