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Chen B.,Fujian Normal University | You W.,Fujian Normal University | Huang J.,Fujian Normal University | Yu Y.,Fujian Normal University | And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2010

The extracellular polysaccharide from Rhodella reticulata was separated from the culture medium followed by concentration and ethanol precipitation, and purified by anion exchange chromatography on DEAE-Sepharose Fast Flow. This study compared the free radical-scavenging property and antioxidant activity with various treatments of crude extracellular polysaccharides of R. reticulata. The results showed that both the crude extracellular polysaccharide and deproteinized crude extracellular polysaccharide gave evidence of the free radical scavenging and antioxidant activity in a dose-dependent manner. The crude extracellular polysaccharide exhibited higher free radical scavenging capacity and better antioxidant activity than the various treatments of crude extracellular polysaccharide samples. The superoxide anion radical scavenging ability of various samples was significantly higher compared to standard antioxidant (α-tocopherol). These results indicate that the extracellular polysaccharide of R. reticulata is a potent natural antioxidant. © Springer Science+Business Media B.V. 2009. Source

Chen H.,Fujian Agriculture and forestry University | Chen H.,Xiamen Ocean Vocational College | Liu Z.,Fujian Agriculture and forestry University | Liu Z.,Fisheries Research Institute of Fujian | And 3 more authors.
Journal of the Science of Food and Agriculture | Year: 2013

Background: The spoilage bacterial community in oyster gill was investigated during storage at 4, 10 and 20°C. Aerobic plate counts and pH values were determined. Total bacterial DNA was extracted from oyster gill and bulk cells of plate count media. The major bacterial species during fresh or different temperatures storage were determined by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Results: The initial aerobic plate count in oyster gill reached 6.70 log CFU g-1. PCR-DGGE fingerprinting analysis of the 16S rRNA gene V3 region revealed that most of the strains in fresh oyster gill belonged to the genera Lactococcus and Enterobacter. The major spoilage bacteria at a storage temperature of 20°C were Leuconostoc pseudomesenteroides, an uncultured bacterium, Cytophaga fermentans, Lactococcus lactis, Pseudoalteromonas sp., Enterococcus mundtii, Clostridium difficile and an uncultured Fusobacteria; those at 10°C were Lactococcus spp., Lactobacillus curvatus, Weissella confusa and C. difficile; those at 4°C were Lactococcus, Weissella, Enterobacter and Aeromonas. The other minor species were L. curvatus, Pseudomonas sp. and E. mundtii. Lactococcus spp. was the most common main spoilage bacteria in oyster gill during chilled storage. Conclusion: PCR-DGGE revealed the complexity of the bacterial microbiota and the major bacteria species in oyster gill for fresh and storage. © 2013 Society of Chemical Industry. Source

Luo D.-L.,Fisheries Research Institute of Fujian
Chinese Journal of Applied Ecology | Year: 2011

Based on the ecological investigation data in September and October 2007, the status of the marine ecological environment of Nanri Archipelago, Fujian Province in summer was diagnosed and assessed from the aspects of sea water quality, nutrient structure and levels, and biodiversity. The comprehensive quality index method was used for the assessment of the marine ecological environment, and the rationality of the assessment obtained from different indices was also discussed. The sea water pH, dissolved oxygen (DO), chemical oxygen demand (COD), and Pb, Cd, Hg, and As concentrations were all within the limit values of the Grade E standard of Sea Water Quality Standard (GB 3097-1997), while the phosphate concentration at 71% stations, inorganic nitrogen at 14% stations, and oil concentration at 7% stations were all above the Grade E standard of Sea Water Quality Standard. Overall, the seawater quality was of better grade, nutrient structure was characterized by N-limited, most of the sea water was at a state of eutrophication, and the diversity index of plankton was at mildly polluted or unpolluted level. The comprehensive quality index indicated that the seawater quality of the Nanri Archipelago was relatively fine. There existed definite differences in the assessment results by using different diagnosis methods, and hence, a relatively objective assessment about marine environmental quality and health status could only be made when the chemical and biological indicators were comprehensively used. Source

He J.,Xiamen University | Qi J.F.,Xiamen University | Qi J.F.,Fisheries Research Institute of Fujian | Feng D.Q.,Xiamen University | Ke C.H.,Xiamen University
Journal of Molluscan Studies | Year: 2016

The marine dreissenid bivalve Mytilopsis sallei is a fouling organism that has invaded habitats outside its original range. Understanding its early development will be useful for early detection in the environment, for species identification in ballast water and for development of control strategies targeted at early life stages, which will help us better manage this important invader. The processes of embryogenesis, shell formation and larval development of M. sallei are described here for the first time by using light and scanning electron microscopy. Released oocytes are 64 μm in diameter. Fertilized eggs were incubated at 27 ± 1 °C. The trochophore, with an apical tuft and a prototroch, developed by 6.0 ± 2.3 h postfertilization (hpf). At 16.5 ± 4.2 hpf, D-shaped veligers with shell length (SL, mean ± SD) of 87.3 ± 8.2 μm appeared, each possessing a velum and a calcified shell. At 2-3 d postfertilization (dpf), the D-shaped veligers developed into umbonate larvae (SL = 111.9 ± 10.7 μm), the last obligate free-swimming veliger stage. Pediveligers (SL = 232.8 ± 37.1 μm) observed at 6-8 dpf could either swim using their velum or crawl with their foot. Pediveligers settled by secreting byssal threads and metamorphosed to plantigrades (SL = 298.7 ± 45.2 μm) 8-10 dpf. It is noteworthy that the larvae of this invasive bivalve are capable of settlement within 10 d. This is the first detailed study of early shell formation of a species of the family Dreissenidae. Shell field invagination appeared during gastrulation, secreting shell material by expanding over both sides in a saddle-shape during the trochophore stage. © The Author 2015. Published by Oxford University Press on behalf of The Malacological Society of London, all rights reserved. Source

Ge H.,Jimei University | Ge H.,Fisheries Research Institute of Fujian | Wang G.,Jimei University | Zhang L.,Jimei University | And 5 more authors.
Gene | Year: 2012

Interleukin receptor-associated kinase (IRAK)-1 binding protein 1 (IRAK1BP1) is a critical factor in preventing dangerous overproduction of proinflammatory cytokines by the innate immune system and in influencing the specificity of TLR responses. In this study, a first molluscan IRAK1BP1 gene, . saIRAK1BP1, was cloned from the small abalone (. Haliotis diversicolor). Its full-length cDNA sequence is 1047. bp, with a 747. bp open reading frame encoding a protein of 249 aa. The molecular mass of the deduced protein is approximately 28.1. kDa with an estimated . pI of 8.87, and shows highest identity (52%) to acorn worm . Saccoglossus kowalevskii. Amino acid sequence analysis revealed that . saIRAK1BP1 shares a conserved SIMPL domain. Quantitative real-time PCR was employed to investigate the tissue distribution of saIRAK1BP1 mRNA, and its expression in abalone under bacteria challenge and larvae at different developmental stages. The saIRAK1BP1 mRNA could be detected in all examined tissues, with the highest expression level in hemocytes, and was up-regulated in gills, kidneys and hemocytes after bacteria injection. Additionally, . saIRAK1BP1 was constitutively expressed at all examined developmental stages. These results indicate that . saIRAK1BP1 play an important role in the adult abalone immune system and might be essential in embryo and larval development in abalone. © 2012 Elsevier B.V. Source

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