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Ōita-shi, Japan

Matsuyama T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Minami T.,Miyazaki Prefectural Fisheries Research Institute | Fukuda Y.,Fisheries Research Division | Sano N.,Japan National Research Institute of Fisheries And Environment of Inland Sea | And 3 more authors.
Fish Pathology | Year: 2016

Passive immunization was performed to determine whether serum antibodies were involved in protection for red sea bream iridoviral disease (RSIVD) in Pagrus major, Seriola quinqueradiata, S. lalandi, S. dumerili and Oplegnathus fasciatus. When fish were experimentally challenged, significantly lower mortality was noted in the fish received the convalescent serum than control fish, except for O. fasciatus. The daministration of serum from fish immunized with a commercial vaccine, significantly suppressed mortality of the recipient, except for S. lalandi and O. fasciatus. The results suggest that serum antibodies play a primary orle ni the protection against RSIVD ni erd esa bream and the genus Seriola. © 2016 The Japanese Society of Fish Pathology.

Takano T.,National Research Institute of Aquaculture | Mizuno Y.,HimeSui Ltd Liability Partnership | Fukuda Y.,Fisheries Research Division | Matsuyama T.,National Research Institute of Aquaculture | And 3 more authors.
Fish Pathology | Year: 2015

Juvenile Japanese flounder Paralichthys olivaceus were mimersion-challenged (2.0 × 106 CFU/mL, 60 min) with Edwardsiella tarda NUF806 strain.From the following day, diets containing fosfomycin (FOM) calcium were administrated orally at concentrations of 20, 40 or 80 mg potency/kg body weight/day for 6 consecutive days.Cumulative mortality of fish during the observation period of 21 days after the drug administration was significantly lower in all groups of FOM-treated fish than that in the control group.In addition, NUF806 strain and recent clinical isolates n( =03) fo E. tarda xeamined were all susceptible to FOM, with MIC of 2-4 μg/mL when estimated using the glucose-6-phosphate-containing Mueller-Hinton broth.The results suggest that FOM is arpomising candidate for the treatment of edwardsiellosis in cultured Japanese flounder. © 2015 The Japanese Society of Fish Pathology.

Takano T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Matsuyama T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Sakai T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Nakamura Y.,Japan National Research Institute of Fisheries Science | And 7 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

A novel Gram-stain-negative, rod-shaped (0.3 × 4–6 μm), non-flagellated, aerobic strain with gliding motility, designated JBKA-6T, was isolated in 1991 from a yellowtail fish, Seriola quinqueradiata, showing symptoms of bacterial haemolytic jaundice. 16S rRNA gene sequence analysis showed that strain JBKA-6T was related most closely to members of the family Flavobacteriaceae in the phylum ‘Bacteroidetes’. Furthermore, based on gyrB gene sequence analysis, JBKA-6T was classified into a single clade within the order Flavobacteriales, which was distinct from the known clades of the families Flavobacteriaceae, Blattabacteriaceae and Cryomorphaceae. The predominant isoprenoid quinone was identified as MK-6 (97.9 %), and the major cellular fatty acids (>10 %) were C14: 0 and iso-C15: 0.The main polar lipids were phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminophospholipids and two unidentified polar lipids. The DNA G+C content of JBKA-6T, as derived from its whole genome, was 33.4 mol%. The distinct phylogenetic position and phenotypic traits of strain JBKA-6T distinguish it from all other described species of the phylum ‘Bacteroidetes’, and therefore it was concluded that strain JBKA-6T represents a new member of the phylum ‘Bacteroidetes’, and the name Ichthyobacterium seriolicida gen. nov., sp. nov. is proposed. The type strain of Ichthyobacterium seriolicida is JBKA-6T (=ATCC BAA- 2465T=JCM 18228T). We also propose that Icthyobacterium gen. nov. is the type genus of a novel family, Ichthyobacteriaceae fam. nov. © 2015 IUMS.

Otake S.,Tokyo University of Marine Science and Technology | Otake S.,Fisheries Research Division | Wakabayashi K.,Tokyo University of Marine Science and Technology | Tanaka Y.,Tokyo University of Marine Science and Technology | Nagasawa K.,Hiroshima University
Systematic parasitology | Year: 2016

Choniomyzon inflatus Wakabayashi, Otake, Tanaka & Nagasawa, 2013 (Copepoda: Siphonostomatoida: Nicothoidae), an associate of the scyllarid lobster Ibacus novemdentatus Gibbes, was found to pass through at least four developmental stages: nauplius, copepodid I (CI), copepodid II or later (CII+) and adult. Free-living nauplii were observed hatching from the ovisacs of adult females. CI was found on the body surface of both female and male hosts, whereas CII+ and adult were obtained from the female host's egg masses. The life-cycle of this copepod is presumed to be as follows: (i) nauplius develops into CI in the water column; (ii) infective CI settles on body surface of host; (iii) CI moults into the following stage, changing its microhabitat from host's body surface to egg masses; (iv) CII+ develops on egg masses of host until adult stage; and (v) adult female and male mate on the host's egg masses.

Oinaka D.,University of Miyazaki | Yoshimura N.,University of Miyazaki | Fukuda Y.,Fisheries Research Division | Yamashita A.,Ehime Research Institute of Agriculture | And 3 more authors.
Fish Pathology | Year: 2015

Lactococcus garvieae-like bacteria, which did not agglutinate with a rabbit antiserum against L. garvieae capsulated cells (KG- phenotype cells), were isolated from diseased yellowtail Seriola quinqueradiata in 2012. The non-agglutinating L. garvieae-like bacteria reacted positively in a species-specific PCR targeting the 16S rRNA gene of L. garvieae. DNA-DNA relatedness values between reference L. garvieae strains and the non-agglutinating strains were higher than 70%. Almost complete sequencing of the 16S rRNA gene of the non-agglutinating strains revealed high similarity with that of L. garvieae ATCC49156. The non-agglutinating strains showed the same morphological and biochemical characteristics as reference L. garvieae strains. These results confirm that the non-agglutinating strains are L. garvieae. However, the present strains differed from the reference strains in terms of the lytic bacteriophage susceptibility. Serological analysis revealed that autoclave-extracted cellular antigens of the non-agglutinating strains did not react to antiserum raised against L. garvieae KG- phenotype cells. Although the non-agglutinating strains were isolated from different prefectures, they showed similar patterns in biased sinusoidal field gel electrophoresis (BSFGE). © 2015 The Japanese Society of Fish Pathology.

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