Fisheries Research and Training Institute

Manawan, Pakistan

Fisheries Research and Training Institute

Manawan, Pakistan

Time filter

Source Type

Hafeez-Ur-Rehman M.,University of Veterinary and Animal Sciences | Ashraf M.,University of Veterinary and Animal Sciences | Abbas F.,University of Veterinary and Animal Sciences | Qureshi I.A.,Fisheries Research and Training Institute | And 3 more authors.
Journal of Animal and Plant Sciences | Year: 2015

The study was conducted to determine the efficacy of various doses of Ovaprim on reproductive behavior of Channa marulius. Fish was fed on 40% protein containing diet at 5% of its body weight for 4 months. On maturity, males weighed were 1100-1340 g and females 1150-1350 g. Two males and two females were randomly paired (8 pairs in total), the 4 groups which emerged were names as control, treatment 1, 2 and 3 (each in 2 replicate tanks). Each male and female received Ovaprim intramuscularly in two installments with an interval of 24 hours. The blood samples were drawn from each sex with an interval of 2 hour for estimation of testosterone, FSH and LH levels. None of the fish spawned even after 48 hours of hormone administration. Ovaries were removed from each fish, weighed and eggs were counted. Fecundity (No.of eggs) was 870.0 ±75.27, 811.2 ±103.30, 900.0 ±95.56 and 940.0 ±39.79 in Control, T1, T2, and T3 respectively. Mean ova diameter ranged from 1.48±0.02 to 1.54±0.01mm. The lowest mean ova diameter (1.48±0.02 mm) was observed in control tank (0.4 ml Ovaprim/kg b.w) while the highest (1.54±0.01 mm) was in T1 (0.5ml Ovaprim/kg b.w.). The highest testosterone level (2.05±0.11 ng ml-1) was observed in T3 after 20 hours of first injection of Ovaprim while the highest FSH level (0.88±0.02 ng ml-1) was observed in the same treatement after the 28 hours of hormone injection. These studies discovered that Ovaprim dosages are inadequate for stimulation of breeding. © 2015, Pakistan Agricultural Scientists Forum. All rights reserved.


Hafeez-Ur-Rehman M.,University of Veterinary and Animal Sciences | Ashraf M.,University of Veterinary and Animal Sciences | Abbas F.,University of Veterinary and Animal Sciences | Iqbal K.J.,Islamia University of Bahawalpur | And 2 more authors.
Pakistan Journal of Zoology | Year: 2015

Effect of different synthetic hormones and/or their analogues have been evaluated on breeding performance of Channa marulius using four different hormonal treatments. Males were injected with various combinations of ovaprim (gonadotropin releasing and dopamine antagonist) with human menopausal gonadotropin (HMG), human chorionic gonadotropin (HCG) and HCG+HMG; whereas, females received combination of ovaprim with HMG and HCG. After 12, 16, 20 and 24 h of first dose, injection of ovaprim were administered and blood testosterone, follicle stimulating hormone and luteinizing hormone were analyzed every 4 h for 48 h. Results indicated that combination of HCG+HMG and ovaprim+HCG were effective and reliable synthetic hormones for induced spawning in C. marulius. Fecundity and egg fertilization rate was highest for HCG+HMG at latency period of 43.20-44.45 h. Blood hormonal levels were non-significant (P<0.05) and increased gradually till 28 h of post injection and then decreased gradually. Fish injected with ovaprim+HCG does not spawn at all. Eggs obtained were yellow, spherical, non-adhesive and translucent. After fertilization, first cleavage was observed within 2 h, second was between 3-4 h and after 4-6 h, a shield appeared inside and two-layered structure appeared with an outer epiblast and inner hypoblast. However, no further development was observed as the eggs succumb to fungal infection. Copyright 2015 Zoological Society of Pakistan.


Ashraf M.,Fisheries Research and Training Institute | Ullah S.,Fish Seed Hatchery | Rashid T.,Fisheries Research and Training Institute | Ayub M.,32 Sanda Road | And 3 more authors.
Pakistan Journal of Nutrition | Year: 2010

Brachionus calyciflorus is commonly found in fresh water ponds. Its production depends on unrelenting supply of Chlorella in sufficient quantity. In the current studies a water sample was collected from fish culture ponds by Wisconsin plankton net (64 μm mesh). The freshly collected stock was concentrated and fractionated by passing through 600, 200, 125, 75 and 38 μm sieves arranged vertically with gradual decrease in pore size. The pure Brachionus calyciflorus were fed on Chlorella available in the laboratory. Algae was gradually replaced by yeast to reduce dependency on labour intensive live food. Maximum number of rotifers 413 ml-1, was observed when they were fed on 160:32 yeastalgae ratio by weight combination. Ciliates and cyclops posed a major threat during culture and frequent crashes were observed due to this menace. Cyclops were selectively eradicated from the rotifer culture at 0.09 DDVP after 20 h of exposure but not ciliates. There was no selective mortality in ciliates at any stage. Nevertheless both ciliates and rotifers were dead at 0.2 ppm. Simple method of cyst preservation is mentioned. © Asian Network for Scientific Information, 2010.


Shaheen T.,Fisheries Research and Training Institute | Akhtar T.,University of Punjab
Turkish Journal of Zoology | Year: 2012

Static renewal exposure of various sublethal concentrations of hexavalent chromium [Cr(VI)] produced a deleterious effect on some hematological and biochemical parameters of Cyprinus carpio with the increase in concentration of metal. C. carpio breeders (W = 500 ± 9.5 g; L = 25.60 ± 2.6 cm) were randomly divided into the control group (n = 33) and treated group (n = 198), with 11 breeders/tank (8.84 m3). The groups were distributed into tanks containing 1 of 7 sublethal concentrations of Cr(VI) (0, 25, 50, 75, 100, 125, and 150 mg/L), with 3 replicates per concentration for 6 months. Monthly Cr(VI) levels in the water were determined with an atomic absorption spectrophotometer. The Cr(VI) induced a significant increase (P < 0.05) in white blood cells, mean corpuscular volume, erythrocyte sedimentation rate, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and acid phosphatase, indicating anemia. However, red blood cells, hemoglobin, packed cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, glucose, protein, and cholesterol decreased significantly (P < 0.05) with the increase in Cr(VI) concentrations from 25 to 150 mg/L. The actual Cr(VI) concentration varied from 36 to 118 mg/L in the tanks exposed to concentrations from 25 to 150 mg/L. The increasing trend was probably due to the toxic effects of the Cr(VI) and activation of the fish immune system to resist against metallic stress and liver damage. The decreasing trend reflected retarded and deteriorated fish health along with severe hemolysis. © TÜBİTAK.


Mirza Z.S.,Fisheries Research and Training Institute | Nadeem M.S.,Fisheries Research and Training Institute | Beg M.A.,Pmas Arid Agriculture University | Qayyum M.,Pmas Arid Agriculture University
Journal of Animal and Plant Sciences | Year: 2012

Present study was aimed to investigate the age structure, growth parameters, natural and fishing mortality coefficients of the common carp (Cyprinus carpio) in Mangla reservoir, Pakistan. To assess the parameters and infer on the degree of exploitation, the fork length frequency data were analyzed using the FISAT (ELEFAN I) computer program. The parameters of the von Bertalanffy growth curve for length were L∞ = 80.33 cm, K= 0.60 yr-1, to= -0.39 year and longevity potential =5.0 years. The instantaneous coefficient of mortality of the population were estimated to be, total mortality (Z)=1.22 years, natural mortality (M) = 0.89 and fishing mortality (F) = 0.33. The stock showed a growth performance (ø) value of 3.588. The current exploitation rate of the species was calculated as 0.27. The analysis of relative yield per recruitment, and relative biomass per recruitment showed that the maximum allowable exploitation rate at Maximum Sustainable Yield (MSY) for the common carp fishery in Mangla reservoir is 0.63 which is more than the currently observed exploitation rate. The results suggest that common carp stock in the reservoir is not overfished and is within safe exploitation limits. It is recommended that regular monitoring program for the stock should be in place and any proposal for a change in exploitation rate should be assessed according to the stock status at that point of time.


Ameer F.,University of Agriculture at Faisalabad | Javed M.,University of Agriculture at Faisalabad | Hayat S.,Fisheries Research and Training Institute | Abdullah S.,University of Agriculture at Faisalabad
Journal of Animal and Plant Sciences | Year: 2013

Growth performance of three age groups viz 60-, 90- and 120-day of two important culture-able indigenous fish species Thaila (Catla catla) and Rohu (Labeo rohita) was studied in glass aquaria under mix exposure of sub-lethal waterborne and dietary metals viz., Cu, Cd and Zn, keeping their control i.e. fish without metal exposure. This experimental trial was run for a period of 90 days at constant temperature (30 °C), pH (7.0) and hardness (200 mgL-1). The treated fish were fed with a diet having 35% digestible protein, 2.90 kcal/g digestible energy and sub-lethal concentration of each metal. All three age groups of both fish species showed statistically variable responses towards increase in wet weights, feed intake and feed conversion ratios under treated and control regimes against toxicity of metals. The 120-day treated fish revealed significantly better average weight gains than other two age groups. The control fish of both the species showed significantly higher weight gains than the treated fish. Three age groups of treated fish showed variable responses towards Cu, Cd and Zn stresses for their average feed intakes. The overall feed intake of control fish was significantly higher than that of treated fish. Feed conversion ratios (FCR) of both treated and control fish species varied significantly among various weeks of the experimental period and control fish exhibited significantly better FCR than the treated fish.


Ashraf M.,Fisheries Research and Training Institute | Ayub M.,Fisheries Research and Training Institute | Sajjad T.,Fisheries Research and Training Institute | Elahi N.,Fish Hatchery Satyana Road | And 2 more authors.
International Journal of Agriculture and Biology | Year: 2010

The studies were conducted to assess the piscicidal activity of 10 locally available plants on freshwater trash fishes. The studies were focused on the laboratory determination of lethal concentrations (LC 50) through a "static bioassay test". Leaf extracts of Nerium oleander (Family Apocynaceae), Cannabium sativum (Bhang, Family Cannabaceae), Datura alba (Family Solanaceae), Adenophyllum spp. (Berri patta, Family Asteraceae), Nicotiana tabacum (Tobacco, Family Solanaceae) and Ricinus communis (Arind, Family Euphobiaceae), while root extracts of Parthenium spp. (Family Compositeae), Calotropis procera (Family Asclepiadaceae), Achyranthes aspera (puth kanda, Family Amaranthaceae) and seed extracts of Ricinus communis and Strychnos were utilized for these studies. Based on the 96 h lethal concentration, Ricinus communis, Datura alba and Strychnos nux vomica (Kutchla, Family Loganiaceae) showed the strongest piscicidal activity to fish. During exposure, fish exhibited discoloration, gulping for air, erratic swimming, loss of reflexes, slow opercular movement and ultimately settling at the bottom motionless. The trial toxicity tests showed that locally available plants have the potential to be used as piscicides, which can be an alternate to an expensive and scarcely available imported rotenone for eradication of undesirable fish species present in fish ponds. © 2010 Friends Science Publishers.


Mirza Z.S.,Fisheries Research and Training Institute | Nadeem M.S.,Pmas Arid Agriculture University | Beg M.A.,Pmas Arid Agriculture University | Malik I.-U.,Pmas Arid Agriculture University
Pakistan Journal of Zoology | Year: 2013

Mangla dam consists of five distinct basins receiving water from two perennial and two nonperennial rivers. Studies were carried out to find spatial and temporal variation in physicochemical characteristics of the surface water in five basins of the dam from January 2009 to December 2010. The result showed that the mean values of temperature, Secchi Depth, pH, Dissolved Oxygen and Conductivity were 22.8°C, 14.5 cm, 7.81, 7.9 mg/L and 441.7/μS/cm. The mean values for total alkalinity, total hardness and total dissolved solids remained 120.0 mg/L, 195.9 mg/L, and 314.0 mg/L respectively. Mean values for nitrates (2.44 mg/L) resulted in higher phytoplankton count (92/ml) in the dam. In general, the water quality followed a cyclical pattern with summers favoring higher primary productivity. Except for pH and dissolved oxygen, almost all the water quality parameters varied significantly (P

Loading Fisheries Research and Training Institute collaborators
Loading Fisheries Research and Training Institute collaborators