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Vinothkumar R.,Fisheries College and Research Institute | Bharti V.S.,Central Institute of Fisheries Education | Vennila A.,Central Institute of Fisheries Education | Kumar H.S.,Central Institute of Fisheries Education | Pandey P.K.,Central Institute of Fisheries Education
Ecology, Environment and Conservation

Isolation and identification of indigenous bacteria are essential for bio-remediating the nitrogenous wastes in aquaculture systems. Nitrite is one of the critical issues inherent to the aquaculture systems due to its toxicity, and hence it is rightly called as "invisible killer". In this study, the heterotrophic nitrifyingdenitrifying bacteria were isolated and characterized from shrimp ponds of Maharashtra. The samples were enriched and bacteria were isolated using Mineral salt media. For molecular identification of the bacteria, the 16S rDNA gene was PCR amplified using universal primers, sequenced and analyzed by BLAST comparison. 15 isolates were identified and selected for nitrate and nitrite removal assay. The nitrate removal activity ranged from 3.012 ± 0.072 to 17.513 ± 0.010 mg of NO3-N/1 in 24h. The nitrite removal activity ranged from 9.385 ± 0.069 to 18.345 ± 0.012 mg NO2-N/1 in 24h. In the present study, the isolates Microbacterium esteraromaticum (M6) and Ochrobactrum intermedium (M13) significantly removed nitrate-N and nitrite-N respectively. The isolated species have the potentials to be used as bioremediator to overcome the toxicity of nitrogenous compounds in aquaculture systems. Copyright © EM International. Source

Gopalakannan A.,Pondicherry University | Gopalakannan A.,Fisheries College and Research Institute | Arul V.,Pondicherry University
Aquaculture International

The present study involved the control of Aeromonashydrophila in Cyprinuscarpio by bacteria Enterococcusfaecium MC13 isolated from fish Mugilcephalus intestine. Antagonistic effects were confirmed against Aeromonashydrophila by cross-streaking and the agar spot method. Probiotic effects of the isolate were confirmed by injection and oral administration to check for nonpathogenicity to fish. All the two probiotics were orally administered to fingerlings of Cyprinus carpio for 60 days through feed. The intestinal load of bacteria and NBT assay were analyzed on 7, 15, 30, and 60th day of treatment. Experimental fish were challenged with Aeromonas hydrophila on 30 and 60th day. The neutrophil activity was higher in E. faecium MC13 fed fish on day 60 (1. 525 ± 0. 379 OD). In respect of relative percentage survival (RPS), the higher protection of 75 and 77. 8% was observed in E. faecium MC13 fed fish on 30 and 60th day of challenge with Aeromonas hydrophila This study identified the bacterial probiont MC13 (Enterococcusfaecium) effectively controlled the Aeromonashydrophila infection in Cyprinuscarpio. © 2011 Springer Science+Business Media B.V. Source

Balakrishnan G.,Annamalai University | Peyail S.,Annamalai University | Kumaran R.,Annamalai University | Theivasigamani A.,Fisheries College and Research Institute | And 3 more authors.
AACL Bioflux

Scientific shrimp culture began in India in the late eighties along the east coast particularly in Andrapradesh and Tamilnadu. Continuous success of shrimp culture was affected by mass mortalities of cultured shrimp in 1994. Thereafter disease infection on survival and production of shrimps get its importance in culture. The present study is the first report on WSSV (white spot syndrome virus) infection in cultured Litopenaeus vannamei (Boone, 1931) in India. WSSV infection was observed on 70 th days of culture due to cross contamination of white spot infected shrimp from the neighboring farm because of birds. Due to this infection within two days the mortality ratio has gone up to 25% in pond 1 and 12% in pond 2. So this present study strongly recommends to every shrimp farmers to go for bird fencing & crab fencing to avoid horizontal contamination, before stocking the good quality seed, then they will have the risk free WSSV culture. Source

Panda D.,Central Inland Fisheries Research Institute | Jawahar P.,Fisheries College and Research Institute | Venkataramani V.K.,D Research and Extension Fisheries
Indian Journal of Fisheries

The sacred chank, Turbinella pyrum is exploited throughout the year along Thoothukudi coast. The life span of T. pyrum was estimated at about 13 years. The growth was observed to be high in the first three years of age, after which the individual attains maturity. Seasonal variation was found to have significant impact on the growth of T. pyrum and reduced growth was observed during the north-east monsoon months i.e., October and November. The growth coefficient (K) was 0.27 which indicates T. pyrum as a slow growing species. The total instantaneous mortality rate (Z) was estimated as 1.73. The fishing mortality (1.33) was found to be more than three times of natural mortality (0.39) for this species, inferring their over-exploitation along Thoothukudi coast. The minimum size at first capture for this species should be fixed above 140 mm shell length (or 70 mm maximum shell diameter) to avoid recruitment overfishing. The chank fishing should be regularized to conserve this precious resource. Source

Pon Saravana Karman A.,Fisheries College and Research Institute | Raj Kumar M.,Central Institute of Fisheries Education | Venkataramani V.K.,Fisheries College and Research Institute
Ecology, Environment and Conservation

The present study deals with age and growth of Etroplus suratensis. Growth equation based on modified von Bertalanffy's equation was arrived. The growth parameters such as L" = 40.4 cm and K = 0.41 were estimated combining both sexes. The spawning peaks one in October and another in February could be observed. The exploitation ratio of 0.28 indicated that the resource of E. suratensis is less exploited along the Tamiraparani riverine system. The life span of E. suratensis was estimated for seven years. The study further recommends that the efforts should be increased to achieve the optimum exploitation (0.5) of the present average annual catch of this species from Tamiraparani riverine system in Western Ghats of India in particular. © EM International. Source

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