Balakrishnan G.,Annamalai University |
Peyail S.,Annamalai University |
Kumaran R.,Annamalai University |
Theivasigamani A.,Fisheries College and Research Institute |
And 3 more authors.
AACL Bioflux | Year: 2011
Scientific shrimp culture began in India in the late eighties along the east coast particularly in Andrapradesh and Tamilnadu. Continuous success of shrimp culture was affected by mass mortalities of cultured shrimp in 1994. Thereafter disease infection on survival and production of shrimps get its importance in culture. The present study is the first report on WSSV (white spot syndrome virus) infection in cultured Litopenaeus vannamei (Boone, 1931) in India. WSSV infection was observed on 70 th days of culture due to cross contamination of white spot infected shrimp from the neighboring farm because of birds. Due to this infection within two days the mortality ratio has gone up to 25% in pond 1 and 12% in pond 2. So this present study strongly recommends to every shrimp farmers to go for bird fencing & crab fencing to avoid horizontal contamination, before stocking the good quality seed, then they will have the risk free WSSV culture.
Chellapan A.,Central Marine Fisheries Research Institute |
Rajagopalsamy C.B.T.,Fisheries College and Research Institute |
Jasmine G.I.,Fisheries College and Research Institute
Indian Journal of Science and Technology | Year: 2013
Anesthetics play an important role in fisheries research and aquaculture and are used to facilitate various handling procedures, such as weighing, sorting, collection of eggs and milt, tagging and medical treatment. Clove oil and benzocaine are approved anesthetic by US Food and Drug Administration. The present study investigates the effect of anesthetics on the respiratory metabolic parameters such as O2 consumption rate, CO2 output rate, NH3-N excretion rate, RQ, AQ and opercular activity of Angel fish, Pterophyllum scalare. The concentration of clove oil and benzocaine were selected based on the time of induction of sedation and recovery of the Angel fish. The concentration of clove oil selected for the Angel fish were 5 ppm, 15 ppm, 30 ppm, 45 ppm, 55 ppm and the benzocaine concentration were 30 ppm, 45 ppm, 50 ppm, 100 ppm, 200 ppm, 300 ppm and 400 ppm. The minimum values of respiratory metabolic parameters in clove oil-anaesthetized Angel fish P.scalare were observed at the concentration of 45 ppm while the same was in 50 ppm in the case of benzocaine treatment. Simulated transportation experiments were conducted for Angel fish and the results suggested that 50ppm benzocaine was the optimum dose of anaesthetics for safe transport of Angel fish.
Panda D.,Central Inland Fisheries Research Institute |
Jawahar P.,Fisheries College and Research Institute |
Venkataramani V.K.,D Research and Extension Fisheries
Indian Journal of Fisheries | Year: 2011
The sacred chank, Turbinella pyrum is exploited throughout the year along Thoothukudi coast. The life span of T. pyrum was estimated at about 13 years. The growth was observed to be high in the first three years of age, after which the individual attains maturity. Seasonal variation was found to have significant impact on the growth of T. pyrum and reduced growth was observed during the north-east monsoon months i.e., October and November. The growth coefficient (K) was 0.27 which indicates T. pyrum as a slow growing species. The total instantaneous mortality rate (Z) was estimated as 1.73. The fishing mortality (1.33) was found to be more than three times of natural mortality (0.39) for this species, inferring their over-exploitation along Thoothukudi coast. The minimum size at first capture for this species should be fixed above 140 mm shell length (or 70 mm maximum shell diameter) to avoid recruitment overfishing. The chank fishing should be regularized to conserve this precious resource.
Gopalakannan A.,Pondicherry University |
Gopalakannan A.,Fisheries College and Research Institute |
Arul V.,Pondicherry University
Aquaculture International | Year: 2011
The present study involved the control of Aeromonashydrophila in Cyprinuscarpio by bacteria Enterococcusfaecium MC13 isolated from fish Mugilcephalus intestine. Antagonistic effects were confirmed against Aeromonashydrophila by cross-streaking and the agar spot method. Probiotic effects of the isolate were confirmed by injection and oral administration to check for nonpathogenicity to fish. All the two probiotics were orally administered to fingerlings of Cyprinus carpio for 60 days through feed. The intestinal load of bacteria and NBT assay were analyzed on 7, 15, 30, and 60th day of treatment. Experimental fish were challenged with Aeromonas hydrophila on 30 and 60th day. The neutrophil activity was higher in E. faecium MC13 fed fish on day 60 (1. 525 ± 0. 379 OD). In respect of relative percentage survival (RPS), the higher protection of 75 and 77. 8% was observed in E. faecium MC13 fed fish on 30 and 60th day of challenge with Aeromonas hydrophila This study identified the bacterial probiont MC13 (Enterococcusfaecium) effectively controlled the Aeromonashydrophila infection in Cyprinuscarpio. © 2011 Springer Science+Business Media B.V.
Vinothkumar R.,Fisheries College and Research Institute |
Bharti V.S.,Central Institute of Fisheries Education |
Vennila A.,Central Institute of Fisheries Education |
Kumar H.S.,Central Institute of Fisheries Education |
Pandey P.K.,Central Institute of Fisheries Education
Ecology, Environment and Conservation | Year: 2016
Isolation and identification of indigenous bacteria are essential for bio-remediating the nitrogenous wastes in aquaculture systems. Nitrite is one of the critical issues inherent to the aquaculture systems due to its toxicity, and hence it is rightly called as "invisible killer". In this study, the heterotrophic nitrifyingdenitrifying bacteria were isolated and characterized from shrimp ponds of Maharashtra. The samples were enriched and bacteria were isolated using Mineral salt media. For molecular identification of the bacteria, the 16S rDNA gene was PCR amplified using universal primers, sequenced and analyzed by BLAST comparison. 15 isolates were identified and selected for nitrate and nitrite removal assay. The nitrate removal activity ranged from 3.012 ± 0.072 to 17.513 ± 0.010 mg of NO3-N/1 in 24h. The nitrite removal activity ranged from 9.385 ± 0.069 to 18.345 ± 0.012 mg NO2-N/1 in 24h. In the present study, the isolates Microbacterium esteraromaticum (M6) and Ochrobactrum intermedium (M13) significantly removed nitrate-N and nitrite-N respectively. The isolated species have the potentials to be used as bioremediator to overcome the toxicity of nitrogenous compounds in aquaculture systems. Copyright © EM International.
Pon Saravana Karman A.,Fisheries College and Research Institute |
Raj Kumar M.,Central Institute of Fisheries Education |
Venkataramani V.K.,Fisheries College and Research Institute
Ecology, Environment and Conservation | Year: 2014
The present study deals with age and growth of Etroplus suratensis. Growth equation based on modified von Bertalanffy's equation was arrived. The growth parameters such as L" = 40.4 cm and K = 0.41 were estimated combining both sexes. The spawning peaks one in October and another in February could be observed. The exploitation ratio of 0.28 indicated that the resource of E. suratensis is less exploited along the Tamiraparani riverine system. The life span of E. suratensis was estimated for seven years. The study further recommends that the efforts should be increased to achieve the optimum exploitation (0.5) of the present average annual catch of this species from Tamiraparani riverine system in Western Ghats of India in particular. © EM International.
Betsy C.J.,Fisheries College and Research Institute |
Kumar J.S.S.,Fisheries College and Research Institute
Journal of Applied Aquaculture | Year: 2015
Cryopreservation of fish gametes can help in producing quality fish seeds. Success of cryopreservation is evaluated by the post-thaw motility of the spermatozoa. The changes in the seminal plasma during cryopreservation would alter the energy supply for the motility of the spermatozoa, and thus energy supplementation is found to be useful during cryopreservation. Cyprinus carpio spermatozoa were cryopreserved along with egg yolk as a co-cryoprotectant after 1:100 dilution with 0.85% physiological saline as extender and DMSO as cryoprotectant (85:15). The diluents contained egg yolk at three different concentrations, viz., T1 (5%), T2 (10%), and T3 (15%). The diluted milt was equilibrated for 10 min at 5°C and loaded into 0.25 ml straws. The loaded straws were then frozen with LN2 vapor for 5 min and immersed in liquid nitrogen. Observations were made once in 7 days for 42 days on motility parameters based on which the duration, score, pattern, and percentage were determined. There were significant differences in the motility duration between treatments, and egg yolk at 5% (T1) concentration was found to support the cryopreserved spermatozoa better than the other concentrations; the difference in motility duration was statistically significant (P > 0.005). © , Copyright © Taylor & Francis Group, LLC.
Hema K.,Fisheries College and Research Institute |
Velayutham P.,Fisheries College and Research Institute |
Sukumar D.,Tamil University |
Sundaramoorthy B.,Fisheries College and Research Institute |
Athithan S.,Fisheries College and Research Institute
Biochemical and Cellular Archives | Year: 2016
An attempt was made to explore the possibilities of better utilization of Lizard fish (Saurida tumbil) for the development of valuable imitation shrimp products. Minced meat, surimi and imitation shrimp products were prepared from Lizard fish. The aluminium shrimp mould was used and to develop the imitation shrimp products. In the present study, the imitated product was observed for the wholesomeness.
George M.R.,Fisheries College and Research Institute |
John K.R.,Fisheries College and Research Institute |
Mansoor M.M.,Fisheries College and Research Institute |
Saravanakumar R.,Fisheries College and Research Institute |
And 2 more authors.
Journal of Fish Diseases | Year: 2015
We investigated mass mortalities of koi, Cyprinus carpio Linnaeus, 1758, experienced in South Indian fish farms by virus isolation, electron microscopy, PCR detection, sequencing of capsid protein gene and transmission studies. Samples of moribund koi brought to the laboratory suffered continuous mortality exhibiting swimming abnormalities, intermittent surfacing and skin darkening. Irido-like virus was isolated from the infected fish in the indigenous snakehead kidney cell line (SNKD2a). Icosahedral virus particles of 100 to 120 nm were observed in the infected cell cultures, budding from the cell membrane. Virus transmission and pathogenicity studies revealed that horizontal transmission occurred associated with mortality. PCR analysis of infected fish and cell cultures confirmed the presence of Ranavirus capsid protein sequences. Sequence analysis of the major capsid protein gene showed an identity of 99.9% to that of largemouth bass virus isolated from North America. Detection and successful isolation of this viral agent becomes the first record of isolation of a virus resembling Santee-Cooper Ranavirus from a koi and from India. We propose the name koi ranavirus to this agent. © 2014 John Wiley & Sons Ltd.
John K.R.,Fisheries College and Research Institute |
George M.R.,Fisheries College and Research Institute |
Iyappan T.,Fisheries College and Research Institute |
Thangarani A.J.,Fisheries College and Research Institute |
Jeyaseelan M.J.P.,Fisheries College and Research Institute
Journal of Fish Diseases | Year: 2010
To detect genomic variation of white spot syndrome virus (WSSV) isolates from different geographical regions of India, the variable number of the tandem repeat (VNTR) region of the ORF 94 (Thailand WSSV isolate - GeneBank Accession No. AF369029) was analysed using five specific sets of primers. Analysis of 70 WSSV-positive samples showed the presence of 14 different genotypes of WSSV with VNTRs ranging from 2 to 16 tandem repeats with the majority (85.47%) having 6-12 tandem repeats. Occurrence of different genotypes of WSSV was found to be neither correlated to any specific geographical region nor to the different growth stage of the tiger shrimp, Penaeus monodon. Pathogenicity studies conducted with 25 isolates of WSSV revealed the presence of virulent and avirulent strains of WSSV in Indian shrimp farms. However, an unambiguous link could not be established between the different genotypes and their virulence. © 2010 Blackwell Publishing Ltd.