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Ling-Juan Z.,Shanghai University | Jie C.,Shanghai University | Jian L.,Shanghai University | Xiao-Ying L.,Shanghai University | And 6 more authors.
Burns | Year: 2012

Purpose: This study was designed to develop and test a Chinese burn patient-specific quality of life (QOL) scale based on the burn-specific health scale - brief (BSHS - B) to provide an effective assessment tool to measure quality of life in Chinese burn patients. Method: Delphi method was used after the translation, back translation and pre-commissioning tests of BSHS - B, to amend the description of the items for cultural adaptation. The adapted Chinese version (ACV) of the BSHS - B was tested for internal consistency and validity of construct on a group of 271 burn patients from three major burn units in China. Results: Thirty-eight items within six domains of ACV BSHS-B were developed with scoring '0-4' in each item. The mean total score for our study group was 98.36 (standard deviation (S.D.) = 37.86), ranging from 13 to 150. The total Cronbach's alpha value was 0.97 and total split-half reliability was 0.98, demonstrating that the internal consistency of the ACV was very high. Pearson correlations among the six domains of ACV BSHS - B were statistically significant. Exploratory factor analysis results showed that the six-factor function explained 78.30% of total variance; each entry in the corresponding domain had a factor-loading value higher than 0.4, indicating that the scale has a good validity. Conclusion: The ACV of the BSHS - B showed good reliability and validity, and can be used as a tool for assessing the QOL in Chinese burn patients.


Zhu F.,Shanghai University | Zhu F.,Nanchang University | Qiu X.,Shanghai University | Wang J.,Shanghai University | And 5 more authors.
Inhalation Toxicology | Year: 2012

Context: Smoke inhalation injury is the leading cause of acute respiratory failure in critical burn victims. Advances in the treatment of smoke inhalation injury have been limited in the past years. To further explore the pathogenesis, stable and practical animal models are necessary. Objective: To develop a rat model of smoke inhalation injury. Materials and methods: The smoke composition including the particulate matters, irritant gases, chemical carcinogens was measured. The blood gas values, pro-inflammatory and protein concentration in bronchoalveolar lavage fluid and lung wet to dry weight ratio were assayed. Pathological evaluations of pulmonary were performed at 24 h, 96 h, 7 days and 28 days post-injury. MassonGoldner trichrome staining was performed on day 7 and 28 post-injury, along with the measurement of hydroxyproline and collagen I and III. Results: In our present animal model, smoke inhalation caused a significant hypoxemia and CO poisoning. A surge of pro-inflammatory response and microvascular hyperpermeability with neutrophils accumulations were also found in our animal model. At 24 h post-smoke inhalation, the hematoxylin and eosin results exhibited that there were inflammatory exudates and diffuse hemorrhage in the lung tissue with significant edema. With the time going, the lung injuries appeared at alveolar collapse and alveolar septum thickening, which indicated that smoke inhalation further induced damage to lung parenchyma. Specially, the markedly collagen deposition appeared at 28 days post-injury indicated that pulmonary fibrosis happened. Discussion and conclusion: In conclusion, this rat smoke inhalation injury model induced by our novel self-made smoke generator could be used for acute and chronic lung injury experiments. © 2012 Informa Healthcare USA, Inc.


Feng W.,First Peoples Hospital of Zhengzhou City | Jin A.-M.,First Peoples Hospital of Zhengzhou City | Liu J.-E.,First Peoples Hospital of Zhengzhou City | Gou R.-E.,First Peoples Hospital of Zhengzhou City | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Previous studies have successfully prepared nano-hydroxyapatite-poly(3-hydroxybutyrate- hydroxyvalerate)/polyethylene glycol-5-fluorouracil. OBJECTIVE: To discuss the effect of nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/polyethylene glycol-5-fluorouracil composite on bone defect repair and against bone tumor. METHODS: Bone tumor models were induced by injection of VX2 carcinoma cell suspension into the medullary cavity of right tibias in 72 New Zealand white rabbits through the knee joints. Partial resection of the tibia was performed to form bone defects that were adjacent to bone tumors. After modeling, all the rabbits were randomized into experimental and control groups. Nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/ polyethylene glycol-5-fluorouracil and nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/ polyethylene glycol artificial bone were implanted into bone defects, respectively, in the experimental and control groups. RESULTS AND CONCLUSION: (1) Inorganic content was decreased gradually in the two groups, especially in the experimental group. (2) X-ray examination: At 24 weeks after implantation, the implant became smaller than that in the early stage after implantation. However, there were no changes in the implant volume in the control group, and tumor volume in the control group was enlarged progressively. (3) Biomechanical detection: At 24 weeks after implantation, the maximal torque of the lower limbs in the experimental group was significantly higher than that in the control group (P < 0.01). (4) Bone mineral density in the two groups was firstly decreased and then increased at 2-24 weeks after implantation. The changes in the bone mineral density of the experimental group were significant. (6) Tumor volume was decreased in the experimental group and increased in the control group at 24 weeks after implantation. These findings indicate that nano-hydroxyapatite-poly(3-hydroxybutyrate- hydroxyvalerate)/polyethylene glycol-5-fluorouracil can perfectly repair bone defects and exert a remarkable effect against tumor growth.


Zhao K.,Pediatric Hospital | Tan R.-G.,First Peoples Hospital of Zhengzhou City
Current Pediatric Research | Year: 2014

This work aims to investigate the effect of traditional Chinese medicine fast-acting asthma relieving mistura (in Chinese, Suxiao Pingchuan Heji) on serum LTC4 expression in mouse models of asthma. 50 KM mice were randomly divided into Group A (control), group B (asthma model), group C (dexamethasone intervention), group D (fast-acting asthma relieving mistura intervention), and group E (intervention by dexamethasone together with fastacting asthma relieving mistura). The asthma models were established by intraperitoneal injection and aerosol inhalation with ovalbumin. The serum LTC4 level in mouse was determined by ELISA method, and the pathological change of lung tissue was observed underlight microscope. There was a little serum LTC4 expression in group A. The LTC4 level in group B was significantly higher than group A (P < 0.01), and that in group C, D, E was significantly lower than group B, respectively (P < 0.01). The inhibition effect on LTC4 expression in group E was obviously greater than group C and D, respectively (P < 0.01), with no significant difference between group C and D (P > 0.05). There was obvious change of inflammatory cell response in group B, with relatively mild inflammatory change in group C, D and E. No inflammatory change was found in group A. Over-expression of LTC4 is closely related to the occurrence and development of asthma. Fast-acting asthma relieving mistura can inhibit the over-expression of LTC4, thus preventing the further development of airway inflammation in asthma. © 2014, Scientific Publishers of India. All rights reserved.


Shen S.,Zhengzhou University | Yue H.,First Peoples Hospital of Zhengzhou City | Li Y.,Zhengzhou University | Qin J.,Zhengzhou University | And 3 more authors.
Tumor Biology | Year: 2014

MicroRNAs (miRNAs) have been integrated into cancer development and progression, because they repress translation of target genes which can be tumor suppressors and oncogenes. A number of miRNAs have been found to be closely related to human non-small cell lung cancer (NSCLC). However, the roles of miR-136 in NSCLC are still largely unknown. Here, we show that miR-136 is significantly upregulated in human NSCLC primary tumors and cell lines compared to their nontumor counterparts. Suppression of miR-136 expression in NSCLC cell line A549 inhibited both anchorage-dependent and anchorage-independent proliferation. Further studies showed that suppression of miR-136 expression attenuated phosphorylation of extracellularsignal-regulated kinase 1/2 (Erk1/2). We found that serine/ threonine protein phosphatase 2A 55 kDa regulatory subunit B α isoform (PPP2R2A, also known as B55α) was a direct target of miR-136, and suppression of miR-136 expression led to a robust increase in both mRNA and protein levels of PPP2R2A.We found thatmiR-136 promoted phosphorylation of Erk1/2 through inhibition of PPP2R2A expression, and forced overexpression of PPP2R2A abrogated promotion of Erk1/2 phosphorylation by miR-136. Moreover, forced overexpression of PPP2R2A abrogated the promoting effect of miR-136 on cell growth and led to a reduced growth rate of NSCLC cells. Our findings indicate that miR-136 promotes Erk1/2 phosphorylation through targeting PPP2R2A in NSCLC cells and suggest that it may serve as a therapeutic target in NSCLC therapy.


Fu S.,Zhengzhou University | Sun X.,Zhengzhou University | Dlykan A.,Zhengzhou University | Jia Y.,Zhengzhou University | And 5 more authors.
Life Science Journal | Year: 2012

Objecttive. To investigate the oncogenicity change of HL-60 cells with silent nucleostemin gene in nude mice and the role of Nucleostemin (NS) specific short hairpin RNA (NS-shRNA) for the anti-leukemia effect in nude mice xenograft tumor model. Methods. HL-60 cells were taken as the model, and were directly transfected with one of Nucleostemin short hairpin RNA (NS-shRNA) which its effect of silencing NS gene is remarkable. In addition, negative control group and blank group were set up. The progress of tumors was observed regularly. Tissues of tumor in every group were handled with pathological section and dyed with HE. Determine the NS protein by immunocytochemisty. In addition, the heterogenic nude mice xenograft tumor models of high-oncogenic HL-60 leukemia cells were established. NS-shRNA was synthesized in vitro to prepare lipid inclusion, and was intraperitoneal inoculated into the mice. The volume and weight of the tumor bodys were measured, the slices of xenograft tumor were stained by HE dye, the NS protein inhibiting effect was detected by immunocytochemistry, and the apoptotic cells of HL-60 in the tumor body were examined by Tunel technique. Results. Different groups need different time to progress the tumor. The experimental group need longer than control group, and the tumor was smaller. The final tumor volume of mouse in experimental group was different significantly with other two groups (P<0.05). But the difference between negative control group and blank group was not significant (P>0.05). Under microscope, it showed that interstitial connective tissue and blood vessels were fewer than other two groups, and the cells arranged becomes loosely. HL-60 cells were not uniform. The cells with karyorrhexis and small nucleus increased. Pykno-levels of nuclear chromatin were not uniform and tumor giant cells decreased. All mice in our study were successfully transplanted by high-oncogenic HL-60 leukemia cells, and the volume of the tumors was even smaller. After treated with NS-shRNA lipid inclusion for 13 days, the tumor volume, weight and NS protein in the tumor cells were statistically lower than control groups. Large areas of patchy destroyed of tumor tissue and "apoptosis character" changes appeared in treated group. A great deal of apoptotic cells appeared in tumor tissue after therapy, detected by Tunel technique. Conclusion. The oncogenicity of HL-60 cells with silent nucleostemin gene was decreased. It is likely related to the change of cells′ biological characters. The anti-leukemia effect of NS-shRNA in nude mice xenograft tumor model is significant; one of the mechanisms probably induce the apoptosis of leukemia cells by the down-regulation of NS expression.


Xu B.,Zhengzhou University | Zhou L.,First Peoples Hospital of Zhengzhou City | Zhang Z.-F.,Zhengzhou University | Liu X.-Z.,Zhengzhou University
Neurosurgery Quarterly | Year: 2015

Objective: To explore the expressions and clinical significance of murine double-minute 2 (MDM2) and p21WAF1 proteins in human glioma. Methods: The expressions of MDM2 and p21WAF1 proteins were, respectively, detected with immunohistochemistry S-P method in glioma and normal brain tissue, and then the relations of their expressions with clinical and pathologic parameters were analyzed. Results: The expressions of MDM2 and p21WAF1 proteins were significantly higher in glioma than in normal brain tissue (P < 0.05). With the increase in the pathologic grading of glioma, MDM2 protein expression was significantly increased (P < 0.05) and p21WAF1 protein expression was significantly decreased (P < 0.05). The expressions of MDM2 and p21WAF1 proteins were related to survival rate and there was a correlation between MDM2 and p21WAF1 (P < 0.05). Conclusions: MDM2 and p21WAF1 proteins play an important role in the genesis and development of glioma, and they may be used as indicators to assess the prognosis of glioma. Copyright © 2014 Wolters Kluwer Health, Inc. All rights reserved.

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