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Gao M.,Zhengzhou University | Liu L.,First Peoples Hospital of Zhengzhou | Li S.,Zhengzhou University | Zhang X.,Zhengzhou University | And 2 more authors.
Oncology Reports | Year: 2015

In the present study, we evaluated the mechanisms of CDC42 in association with the microRNA-137 (miR- 137)-induced inhibition of human hepatocellular carcinoma (HCC). The gene expression levels of miR-137 were evaluated in HCC cell lines. Direct association of miR-137 with its downstream target, cell division control protein 42 (CDC42), was evaluated by dual-luciferase assay, western blot analysis and correlation analysis using clinical tumor samples. In the HCC HuH7 and MHCC97L cell lines, miR-137 was upregulated to inhibit cell proliferation and metastasis in vitro and tumor growth in vivo. CDC42 was overexpressed in the HuH7 and MHCC97L cells to evaluate its effect on the miR-137-mediated antitumor effects. Furthermore, possible crosstalk between CDC42 and another miR-137 target gene, AKT2, was evaluated by co-overexpressing CDC42 and AKT2 in the HuH7 and MHCC97L cells and examining their effects on miR- 137-mediated HCC regulation. miR-137 was confirmed to be downregulated in the HCC cell lines. Dual-luciferase assay showed that CDC42 was directly targeted by miR-137, and western blotting showed that CDC42 was downregulated by miR-137 upregulation in the HuH7 and MHCC97L cells. In human tumors, the expression levels of CDC42 and miR-137 were inversely correlated. The inhibitory effects of miR-137 on HCC proliferation, metastasis and in vivo tumor growth were all ameliorated by CDC42 overexpression. Furthermore, co-overexpression of AKT2 in addition to CDC42 additively reduced the inhibition of miR-137 on HCC proliferation and metastasis, suggesting two independent pathways of CDC42 and AKT2 in miR-137-mediated HCC regulation. Our study demonstrated that CDC42 independently regulated the antitumor effects of miR-137 in human HCC. Source

Zhang Y.-R.,Zhengzhou University | Shi L.,Zhengzhou University | Wu H.,First Peoples Hospital of Zhengzhou | Tang D.-D.,Zhengzhou University | And 4 more authors.
Tumori | Year: 2014

Aims and background. To ascertain the value of the detection of urinary modified nucleosides in the early diagnosis and prognostic monitoring of urothelial bladder cancer. Methods. One hundred seventeen patients with urothelial bladder carcinoma and 66 healthy volunteers were included in the study. High-performance liquid chromatog-raphy/electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOF-MS) was used to measure the levels of urinary modified nucleo-sides in the bladder cancer and control groups. Postoperative monitoring was done every 3 months in patients with noninvasive carcinoma; 85 patients attended the 1-year follow-up visit. Results. The levels of m1A, ac4C, O6-MeG and 1-MeI were significantly higher in cases than controls (P <0.05). The highest sensitivity (92.45%) and specificity (87.50%) were obtained when 1-MeI detection was combined with m1A detection. The m1A and 1-MeI levels 3 months after operation in both patient groups were significantly lower than the preoperative levels (P <0.01). The no-recurrence group subsequently maintained low levels, but in the recurrence group the levels rose again almost to preoperative values. At 6, 9 and 12 months after operation, the m1A and 1-MeI levels of the recurrence group were higher than those of the no-recurrence group and the control group (P <0.01). Conclusions. Urinary modified nucleosides might become novel tumor markers that will facilitate the clinical management and will be helpful in the diagnosis and follow-up of urothelial bladder cancer. m1A and 1-MeI appear to be most promising for clinical use and be worthy of further study in the near future. Source

Bai G.,Xian Jiaotong University | Fu F.,First Peoples Hospital of Zhengzhou | Tang Y.,Xian Jiaotong University | Wang Y.,Xian Jiaotong University
Journal of Obstetrics and Gynaecology Research | Year: 2013

Aim: To investigate the effect and mechanism of hepatitis B virus (HBV) infection on the human choriocarcinoma cell line, JEG-3, in relation to apoptosis and intrauterine infection. Material and Methods: HBV-DNA serum was used to infect the choriocarcinoma cell line, JEG-3, in vitro. Real-time fluorescence quantitative PCR (RT-PCR) was then employed to detect intracellular replication of HBV DNA. Cells were also stained with Annexin-V and propidium iodide (PI) to identify the stages of apoptosis following infection. In addition, reverse transcription PCR was used to detect intracellular HBx mRNA levels, and Western blotting and immunohistochemistry were used to detect changes in the intracellular expression of HBxAg and phosphatidylinositol kinase 3 (PI3K). Flow cytometry was also used to detect the intracellular levels of phosphorylated AKT (pAKT). Results: After JEG-3 cells were infected with HBV in vitro, HBV DNA was detected. The percentage of cells in early and late stage apoptosis also decreased significantly. Expression of HBx mRNA and HBxAg were detected, and intracellular levels of PI3K and pAKT were observed to significantly increase. Conclusion: HBV infected JEG-3 cells in vitro, resulting in an inhibition of early and late stage apoptosis. In addition, the HBxAg/PI3K/pAKT pathway is a possible mechanism mediating this inhibition of apoptosis, and the infection of the placenta by HBV. © 2013 The Authors. Source

Liu L.,First Peoples Hospital of Zhengzhou | Chen X.,Cancer Hospital of Henan Province | Gao M.,Zhengzhou University
Chinese Journal of Clinical Oncology | Year: 2011

Objective: To investigate the response rate and adverse reactions in patients with advanced or metastatic gastric cancer treated with the regimen of Docetaxel in combination with Oxaliplatin and Capecitabine. Methods: Fifty-six patients were treated with the regimen as follows: Docetaxel 75mg/m2, intravenous, day 1; Oxaliplatin 85mg/m2, intravenous, day 2; Capecitabine 1000mg/m2, twice a day, PO, for 14 days, 21 days for each cycle. Evaluation was performed at the end of 2 cyeles. Results: All of these 56 patients were evaluable for response. Six (10.7%) patients achieved complete response (CR), 30 (53.6%) patients had partial response (PR), 12 (21.4%) patients had stable disease (SD), 8 (14.3%) patients had disease progression (DP), with a total response rate ( RR) of 64.3%. The median progression-free survival was 6.2 months (3.6-11.8 months) and the median overall survival time was 11.6 months (5.9-14.6 months). The most common adverse effects were myelosuppression, gastrointestinal toxicities and neurotoxicity. No chemotherapy-related death was observed. Conclusion: Docetaxel combined with Oxaliplatin and Capecitabine is effective for advanced gastric cancer, with tolerable side effects. Source

Wang Y.,Xinxiang Medical University | Tang Z.,Xinxiang Medical University | Wang Y.,First Peoples Hospital of Zhengzhou | Qian X.,Xinxiang Medical University
Life Science Journal | Year: 2013

To detect the expression of E-cadherin and MMP-9 protein in esophageal squamous cell carcinoma (SCC) and investigate their correlations with the occurrence and development of esophageal SCC, SP immunohistochemical method was used to detect the expression of E-cadherin and MMP-9 protein in 124 cases of esophageal SCC, 62 cases of normal esophageal epithelium. The expression of E-cadherin and MMP-9 protein were closely correlated with the infiltration and lymph node metastasis of esophageal SCC (P < 0.05), but were not correlated with the tumor grade, age or gender of the patients (P > 0.05). The expression rate of E-cadherin protein was much higher in normal esophageal epithelium (90.3%) than in esophageal SCC (43.5%). There was significant difference between them (P < 0.01). However, the expression rate of MMP-9 protein was 72.6% in esophageal SCC, 33.9% in normal esophageal epithelium. And there was significant difference between them,too(P < 0.01). The expression of E-cadherin protein was negatively correlated with MMP-9 expression (P<0.01). Conclusively, E-cadherin and MMP-9 may play important roles in the infiltration, metastasis and carcinomatous changes of mucosal epithelium in esophageal carcinoma. United detection of them may be used in the early diagnosis and prognosis judgment of esophageal SCC. Source

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