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Jiangtao G.,First Peoples Hospital Of Zhengzhou | Heping H.,First Peoples Hospital Of Zhengzhou | Zhi'an J.,First Peoples Hospital Of Zhengzhou | Changqing M.,First Peoples Hospital Of Zhengzhou | Peng L.,Shanghai JiaoTong University
Chinese Journal of Andrology | Year: 2014

Objective To study the clinical and pathological features, diagnosis and treatment of malignant mesothelioma of the tunica vaginalis testis. Methods A case of malignant mesothelioma of the tunica vaginalis testis was reported and the related literatures were reviewed. Results The patient was A 63 years old and his B-ultrasound result showed obvious liquid anechoic area around the testis, the tunica vaginalis was resected radically. Pathological analysis of testis tunica vaginalis demonstrated malignant mesothelium-derived carcinoma. Two months later, the patient died. Conclusion Malignant mesothelioma of the tunica vaginalis is a rare disease of the testis with potentially aggressive behavior. Due to the lack of tumor clinic characteristic symptoms and specific tumor maker, correct preoperative diagnosis is extremely difficult. Radical surgical treatment is a major treatment and after operation chemoradiotherapy can improve survival of the patients. Totally the patient has an indicator of poor prognosis. ©, 2014. Shanghai Jiaotong University School of Medicine. All rights reserved.


Liu L.,First Peoples Hospital of Zhengzhou | Chen X.,Cancer Hospital of Henan Province | Gao M.,Zhengzhou University
Chinese Journal of Clinical Oncology | Year: 2011

Objective: To investigate the response rate and adverse reactions in patients with advanced or metastatic gastric cancer treated with the regimen of Docetaxel in combination with Oxaliplatin and Capecitabine. Methods: Fifty-six patients were treated with the regimen as follows: Docetaxel 75mg/m2, intravenous, day 1; Oxaliplatin 85mg/m2, intravenous, day 2; Capecitabine 1000mg/m2, twice a day, PO, for 14 days, 21 days for each cycle. Evaluation was performed at the end of 2 cyeles. Results: All of these 56 patients were evaluable for response. Six (10.7%) patients achieved complete response (CR), 30 (53.6%) patients had partial response (PR), 12 (21.4%) patients had stable disease (SD), 8 (14.3%) patients had disease progression (DP), with a total response rate ( RR) of 64.3%. The median progression-free survival was 6.2 months (3.6-11.8 months) and the median overall survival time was 11.6 months (5.9-14.6 months). The most common adverse effects were myelosuppression, gastrointestinal toxicities and neurotoxicity. No chemotherapy-related death was observed. Conclusion: Docetaxel combined with Oxaliplatin and Capecitabine is effective for advanced gastric cancer, with tolerable side effects.


Bai G.,Xi'an Jiaotong University | Fu F.,First Peoples Hospital of Zhengzhou | Tang Y.,Xi'an Jiaotong University | Wang Y.,Xi'an Jiaotong University
Journal of Obstetrics and Gynaecology Research | Year: 2013

Aim: To investigate the effect and mechanism of hepatitis B virus (HBV) infection on the human choriocarcinoma cell line, JEG-3, in relation to apoptosis and intrauterine infection. Material and Methods: HBV-DNA serum was used to infect the choriocarcinoma cell line, JEG-3, in vitro. Real-time fluorescence quantitative PCR (RT-PCR) was then employed to detect intracellular replication of HBV DNA. Cells were also stained with Annexin-V and propidium iodide (PI) to identify the stages of apoptosis following infection. In addition, reverse transcription PCR was used to detect intracellular HBx mRNA levels, and Western blotting and immunohistochemistry were used to detect changes in the intracellular expression of HBxAg and phosphatidylinositol kinase 3 (PI3K). Flow cytometry was also used to detect the intracellular levels of phosphorylated AKT (pAKT). Results: After JEG-3 cells were infected with HBV in vitro, HBV DNA was detected. The percentage of cells in early and late stage apoptosis also decreased significantly. Expression of HBx mRNA and HBxAg were detected, and intracellular levels of PI3K and pAKT were observed to significantly increase. Conclusion: HBV infected JEG-3 cells in vitro, resulting in an inhibition of early and late stage apoptosis. In addition, the HBxAg/PI3K/pAKT pathway is a possible mechanism mediating this inhibition of apoptosis, and the infection of the placenta by HBV. © 2013 The Authors.


Wang Y.,Xinxiang Medical University | Tang Z.,Xinxiang Medical University | Wang Y.,First Peoples Hospital of Zhengzhou | Qian X.,Xinxiang Medical University
Life Science Journal | Year: 2013

To detect the expression of E-cadherin and MMP-9 protein in esophageal squamous cell carcinoma (SCC) and investigate their correlations with the occurrence and development of esophageal SCC, SP immunohistochemical method was used to detect the expression of E-cadherin and MMP-9 protein in 124 cases of esophageal SCC, 62 cases of normal esophageal epithelium. The expression of E-cadherin and MMP-9 protein were closely correlated with the infiltration and lymph node metastasis of esophageal SCC (P < 0.05), but were not correlated with the tumor grade, age or gender of the patients (P > 0.05). The expression rate of E-cadherin protein was much higher in normal esophageal epithelium (90.3%) than in esophageal SCC (43.5%). There was significant difference between them (P < 0.01). However, the expression rate of MMP-9 protein was 72.6% in esophageal SCC, 33.9% in normal esophageal epithelium. And there was significant difference between them,too(P < 0.01). The expression of E-cadherin protein was negatively correlated with MMP-9 expression (P<0.01). Conclusively, E-cadherin and MMP-9 may play important roles in the infiltration, metastasis and carcinomatous changes of mucosal epithelium in esophageal carcinoma. United detection of them may be used in the early diagnosis and prognosis judgment of esophageal SCC.


Zhang Y.-R.,Zhengzhou University | Zhang Y.-R.,Peoples Hospital of Zhengzhou | Shi L.,Zhengzhou University | Wu H.,First Peoples Hospital of Zhengzhou | And 5 more authors.
Tumori | Year: 2014

Aims and background. To ascertain the value of the detection of urinary modified nucleosides in the early diagnosis and prognostic monitoring of urothelial bladder cancer. Methods. One hundred seventeen patients with urothelial bladder carcinoma and 66 healthy volunteers were included in the study. High-performance liquid chromatog-raphy/electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOF-MS) was used to measure the levels of urinary modified nucleo-sides in the bladder cancer and control groups. Postoperative monitoring was done every 3 months in patients with noninvasive carcinoma; 85 patients attended the 1-year follow-up visit. Results. The levels of m1A, ac4C, O6-MeG and 1-MeI were significantly higher in cases than controls (P <0.05). The highest sensitivity (92.45%) and specificity (87.50%) were obtained when 1-MeI detection was combined with m1A detection. The m1A and 1-MeI levels 3 months after operation in both patient groups were significantly lower than the preoperative levels (P <0.01). The no-recurrence group subsequently maintained low levels, but in the recurrence group the levels rose again almost to preoperative values. At 6, 9 and 12 months after operation, the m1A and 1-MeI levels of the recurrence group were higher than those of the no-recurrence group and the control group (P <0.01). Conclusions. Urinary modified nucleosides might become novel tumor markers that will facilitate the clinical management and will be helpful in the diagnosis and follow-up of urothelial bladder cancer. m1A and 1-MeI appear to be most promising for clinical use and be worthy of further study in the near future.


Lu T.,First Peoples Hospital of Zhengzhou | Li Y.-C.,First Peoples Hospital of Zhengzhou | Li S.-R.,First Peoples Hospital of Zhengzhou | Niu X.-H.,First Peoples Hospital of Zhengzhou | Lou J.-H.,First Peoples Hospital of Zhengzhou
Journal of Dalian Medical University | Year: 2012

[Objective] To investigate the protective effects of edaravone treatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI). [Methods] Fifty-four mice were randomized into 3 groups: normal group (Sham operation), control group (LPS + physiological saline) and experiment group (LPS + Edaravone). The mice in control and experiment group were induced ALI by intra-tracheal LPS solution (2 mg/kg body weight). The mice in experiment group were given Edaravone solution (2 mg/mL) 2.5 mL/kg body weight and the mice in control group were given saline at the same volume. At 24 h post-injury, 6 mice in each group were killed and their lung tissues were isolated and assayed for malon-aldehyde (MDA), superoxide dismutase activity (SOD), TNF-α, IL-1β, IL-6 and histological examination. The remained 12 mice in each group were used for monitoring the survival rate of mice every 12 h for 4 days. [Results] Our experiments exhibited that the survival rate of experiment group was higher than control group (P < 0.05). After LPS stimulation, MDA content in the lung tissues was increased from (1.41 ± 0.119) nmol/mg to (4.48 ± 0.159) nmol/mg, whereas it was decreased to (2.56 ± 0.157) nmol/mg in the Edaravone treatment group (P < 0.01). After LPS stimulation, SOD activity in the lung tissues was decreased from (12.86 ± 1.49) U/mg to (8.95 ± 1.02) U/mg, whereas it was increased to (8.95 ± 1.02) nmol/mg in the Edaravone treatment group (P < 0.01). After LPS stimulation, TNF-α, IL-1β and IL-6 content in the lung tissues was increased from (47.89 ± 4.71) pg/mg, (79.39 ± 3.45) pg/mg and (81.9 ± 6.39) pg/mg to (300.48 ± 12.18) pg/mg, (717.99 ± 35.01) pg/mg and (428.99 ± 21.89) pg/mg, whereas they were decreased to (191.84 ± 6.43) pg/mg, (236.87 ± 13.46) pg/mg and (136.92 ± 12.47) pg/mg in the Edaravone treatment group. Edaravone treatment further attenuated lung edema, inflammatory cell infiltrations, widened alveolar septum, and diffuse hemorrhage. [Conclusions] In conclusion, our data demonstrated that ameliorated LPS-induced ALI.


Feng Z.,First Peoples Hospital of Zhengzhou | Jing Z.,First Peoples Hospital of Zhengzhou | Liu H.,Zhengzhou University | Liao S.,First Peoples Hospital of Zhengzhou | And 5 more authors.
Chinese Journal of Medical Genetics | Year: 2015

Objective To explore the possible roles of polymorphisms of SPO11 and glutathionine S-transferase (GST) genes in idiopathic male infertility in a ethnic Han Chinese population from Henan. Methods Multiplex PCR and DNA sequencing were performed to determine the SPOll c. 517>T (rs28368082) and GST genes (GSTM1, GSTT1, GSTP1) polymorphisms in 216 idiopathic male infertility cases and 198 normal samples. Results The frequencies of the SPOll CC and CT genotypes were 87.5% (189/216) and 12.5% (27/216) in the patients, and 97.5% (193/198) and 2.5% (5/198) in the controls, respectively. The frequencies of SPOll CC and CT genotypes, the A>G transition at nucleotide 313 in the exon 5 of the GSTP1 gene, and the frequencies of combined genotypes GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and SPOll (CT) were significantly different between the two groups (P<0.05). Conclusion The rs28368082 polymorphism of the SPOll gene, the A>G transition at nucleotide 313 in the exon 5 of the GSTPl gene, and the combined genotypes of GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and SPO11 (CT) may be associated with idiopathic male infertility in ethnic Han Chinese.


Gao M.,Zhengzhou University | Liu L.,First Peoples Hospital of Zhengzhou | Li S.,Zhengzhou University | Zhang X.,Zhengzhou University | And 2 more authors.
Oncology Reports | Year: 2015

In the present study, we evaluated the mechanisms of CDC42 in association with the microRNA-137 (miR- 137)-induced inhibition of human hepatocellular carcinoma (HCC). The gene expression levels of miR-137 were evaluated in HCC cell lines. Direct association of miR-137 with its downstream target, cell division control protein 42 (CDC42), was evaluated by dual-luciferase assay, western blot analysis and correlation analysis using clinical tumor samples. In the HCC HuH7 and MHCC97L cell lines, miR-137 was upregulated to inhibit cell proliferation and metastasis in vitro and tumor growth in vivo. CDC42 was overexpressed in the HuH7 and MHCC97L cells to evaluate its effect on the miR-137-mediated antitumor effects. Furthermore, possible crosstalk between CDC42 and another miR-137 target gene, AKT2, was evaluated by co-overexpressing CDC42 and AKT2 in the HuH7 and MHCC97L cells and examining their effects on miR- 137-mediated HCC regulation. miR-137 was confirmed to be downregulated in the HCC cell lines. Dual-luciferase assay showed that CDC42 was directly targeted by miR-137, and western blotting showed that CDC42 was downregulated by miR-137 upregulation in the HuH7 and MHCC97L cells. In human tumors, the expression levels of CDC42 and miR-137 were inversely correlated. The inhibitory effects of miR-137 on HCC proliferation, metastasis and in vivo tumor growth were all ameliorated by CDC42 overexpression. Furthermore, co-overexpression of AKT2 in addition to CDC42 additively reduced the inhibition of miR-137 on HCC proliferation and metastasis, suggesting two independent pathways of CDC42 and AKT2 in miR-137-mediated HCC regulation. Our study demonstrated that CDC42 independently regulated the antitumor effects of miR-137 in human HCC.


Chen H.-C.,First Peoples Hospital of Zhengzhou | Liang Q.-P.,First Peoples Hospital of Zhengzhou | Yang D.-S.,First Peoples Hospital of Zhengzhou | Fang L.-F.,First Peoples Hospital of Zhengzhou
Cancer Research and Clinic | Year: 2013

Objective: To investigate the relationships and clinicopathologic features of the expressions of cytochrome P450 2E1 (CYP2E1) and PC cell-derived growth factor (PCDGF) in esophageal squamous cell carcinoma (ESCC), and to determine the role of CYP2E1 and PCDGF in angiogenesis. Methods: The expression levels of CYP2E1 and PCDGF in 42 surgical cancer specimens and 20 adjacent normal esophageal mucosa specimens from patients with ESCC were detected by immunohistochemistry. Results: The positive expression rates of CYP2E1 and PCDGF in ESCC were 83.3 % (35/42) and 88.1 % (37/42), respectively, which were obviously higher than those of normal mucosa (P < 0.05). Expression of PCDGF was correlated with the degree of histological differentiation (r = 0.444, P < 0.05), depth of tumor invasion (r = 0.332, P < 0.05), lymph node metastasis (r = 0.476, P < 0.05), and TNM classification (r = 0.450, P < 0.05). The expression of CYP2E1 was negatively correlated with tumor tissue differentiation (r = -0.518, P < 0.05), and positively correlated with depth of invasion in ESCC (r = 0.388, P < 0.05). The expression of CYP2E1 was related to that of PCDGF in the tumor (r = 0.483, P < 0.05). Conclusion: The expressions of CYP2E1 and PCDGF are synergistically involved in tumor growth, infiltration and metastasis. Overexpression of CYP2E1 and PCDGF can be used as the important predictors for evaluating the biological behavior of ESCC and predicting prognosis of patients.


Lou P.,First Peoples Hospital of Zhengzhou | Lu H.-b.,First Peoples Hospital of Zhengzhou | Qiang L.,First Peoples Hospital of Zhengzhou
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Gateway-Wingspan stent system was certified by the U. S. FDA in 2005 as a nickel-titanium alloy self-expanding stent for the treatment of symptomatic artery stenosis, which was approved to be applied formally in China in 2007. OBJECTIVE: To evaluate the safety and short-term effect of endovascular implantation of nickel-titanium alloy self-expanding Wingspan stent for symptomatic artery stenosis. METHODS: Totally 13 patients with 18 lesions diagnosed as symptomatic artery stenosis were treated with endovascular Wingspan stent based on the Gateway saccule self-expansion. The vascular stenotic lesions involved middle cerebral artery in 11 cases, internal carotid artery in 4 cases, vertebral artery in 2 cases, and basilar artery in 1 case. The length of vascular stenotic lesions was 5-15 mm with a mean incidence of (85. 3±10. 0)%. RESULTS AND CONCLUSION: All of the 18 stents in the 13 patients were placed successfully in one implantation, accounting for 100%. The stenosis residue was (30. 5±12. 0)% immediately after implantation; after 12 months follow-up, there was no stroke attack or transient cerebral infarction. The vascular angiography showed no stenosis. After stent implantation, there was no arterial dissection, tear of arterial intima, and acute thrombosis in 13 cases. The stents showed no displacement or rupture. One case appeared to have hypotension and one case had ecchymoma within 3 days after stent implantation, and the symptoms disappeared after treatment. The experimental findings demonstrate that endovascular implantation of nickel-titanium alloy self-expanding Wingspan stent is a safe and effective treatment for intracranial symptomatic artery stenosis, with good short-term efficacy.

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