First Peoples Hospital of Zhangjiagang

Zhangjiagang, China

First Peoples Hospital of Zhangjiagang

Zhangjiagang, China
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Shen D.,First Peoples Hospital of Zhangjiagang | Bian X.-H.,Jiangsu Tumor Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2010

OBJECTIVE: To investigate the clinical effect of 3D-CRT combined with FOLFOX regimen in the treatment of stage III of esophageal cancer. METHODS: Totally 75 patients with stage III esophageal cancer were randomly divided into treatment group (n=40) and control group (n=35). Patients in treatment group received concurrent chemoradiation therapy (oxaliplatin +CF+5-FU). Radiotherapy using 6 MV X ray, the radiation dose ranged from 60 to 64 Gy, in daily 2.0 Gy/f, 5 times weekly. Patients in control group only received chemotherpy in control group. RESULTS: The efficiency rate(RR) of treatment group was 80.0%, 1,2,3-year survival rates were 67.5%, 52.5% and 42.5%, significantly higher than the reatment group (54.3% and 45.7%, 31.4%, 22.9%, P<0.05), the incidence of radiation esophagitis in the control group up to 100.0%, mainly grade I, II degree, significantly higher than that of treatment group (0, P<0.05), but the symptoms can alleviate after givening symptomatic treatment. CONCLUSION: Concurrent chemotherapy can improve the efficacy and 1,2 and 3-year survival rate, but the radiation esophagitis significantly increasing.


Song Y.,Soochow University of China | Song Y.,First Peoples Hospital of Zhangjiagang | Tian Y.,Soochow University of China | Zuo Y.,First Peoples Hospital of Zhangjiagang | And 3 more authors.
Molecular Medicine Reports | Year: 2013

Human hedgehog-interacting protein (HHIP) and protein patched homolog (PTCH) are two negative regulators of the hedgehog signal, however, the mechanism of action in gastric cancer is unknown. Methylation of TSG promoters has been considered as a causative mechanism of tumorigenesis. In the present study, we first determined the expression of PTCH and HHIP mRNA and protein in gastric cancer tissues and adjacent normal tissues, and then detected methylation of the two genes to associate their expression and gene promoter methylation in gastric cancer. Expression in gastric cancer tissues and the cancer cells (AGS) were evaluated by reverse transcription-PCR (RT-PCR), qRT-PCR and IHC, while the methylation expression was valued by methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP). Cell viability and apoptosis were analyzed by MTT assay and flow cytometry following treatment with 5-aza-dc. Results showed that PTCH and HHIP expression was reduced in gastric cancer tissues that were not associated with clinical features. Moreover, methylation of the promoters was reversely correlated with the expression. Following treatment with 5-aza-dc, AGS reduced cell viability and induced apoptosis, which is associated with upregulation of HHIP expression. The data demonstrated that loss of expression of HHIP and PTCH is associated with the methylation of gene promoters. In addition, 5-aza-dc-induced apoptosis correlated with the upregulation of HHIP expression in AGS. The findings demonstrated that the PTCH and HHIP genes may be novel targets for the control of gastric cancer. Copyright © 2013 Spandidos Publications Ltd.


Xi H.-J.,First Peoples Hospital of Zhangjiagang
Journal of Clinical Neurology (China) | Year: 2012

Objective: To approach the effects of Rosuvastatin calcium on blood lipids and carotid artery atherosclerotic in patients with transient ischemic attack (TIA). Methods: Sixty-four TIA patients with carotid atherosclerosis were randomly divided into therapy group (32 cases) and control group (32 cases). On the basic of the treatment including Nimodipine, Aspirin, cytidine diphosphate choline, etc. in the 2 group, the patients in therapy group received Rosuvastatin calcium 10 mg/d for 6 months. Pre and post treatment, the levels of blood lipids were detected. The carotid intima-media thickness (IMT) and the area of carotid atherosclerotic plaques were measured by ultrasonic. Followed-up for 6 months, the incidence of cerebrovascular disease and adverse drug reaction were record. Results: The levels of blood triglyceride, cholesterol and low density lipoprotein were significantly lower, and the carotid IMT and area of plaque were significantly less at post treatment in therapy group than pre treatment and control group (all P<0.05), while there was no significant difference in the control group between pre and post treatment. The incident of cerebrovascular disease (9.4%) in therapy group was significantly lower than that in control group (37.5%) (χ 2 = 7.108, P < 0.05). There was no adverse reaction in the two groups. Conclusion: Rosuvastatin calcium can effectively reduce the blood lipid level, improve the carotid atherosclerosis and reduce the incidence of cerebrovascular disease.


Song Y.,First Peoples Hospital of Zhangjiagang | Zuo Y.,First Peoples Hospital of Zhangjiagang | Tu J.,First Peoples Hospital of Zhangjiagang | Feng Y.,First Peoples Hospital of Zhangjiagang | Xu Z.,First Peoples Hospital of Zhangjiagang
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To Observe the methylation level of CpG island in human hedgehog interacting protein (HHIP) gene in gastric cancer tissues, peritumoral tissues and AGS cells, and to explore the relationship between the methylation level of CpG island and the tumorigenesis of gastric cancer. Methods: The expressions of HHIP mRNA in 30 human gastric carcinnoma tissues, peritumoral tissues and gastric carcinoma AGS cells were detected by RT-PCR. The expressions of HHIP and the methylation level of promoter region of HHIP gene in gastric carcinnoma tissues and peritumoral tissues were detected by immunohistochemical staining and methylation specific PCR (MSP), respectively. Before and after the treatment of methyl transferase inhibitor 5-Aza-2'-deoxycitydine (5-Aza-dc), the HHIP mRNA expression, methylation level of promoter region and methylation sites locus on CpG island in AGS cells were detected by RT-PCR, MSP and bisulfite sequencing PCR(BSP), respectively. The correlation between the methylation status of CpG island at HHIP promoter region and HHIP mRNA expression level was analyzed. Results: The expression of HHIP mRNA (0.82±0.38 vs 1.60±0.26, P=0.000) and HHIP (0.5±0.03 vs 0.8±0.27, P<0.05) in gastric carcinoma was significantly lower than that in the peritumoral tissues. No significant correlation was observed between the expression of HHIP mRNA or protein and age, sex, TNM stage, differentiation degree and lymph node metastasis (P>0.05). The methylation level of HHIP gene promtor in the peritumoral tissues was significantly higher than that in the gastric cancer tissues and AGS cells (\[17.7±3.59\]% vs \[62.9±6.14\]%, \[99.7±0.67\]%; all P<0.05). Compared with that before 5-Aza-dc treatment, the HHIP mRNA expression in AGS cells was significantly increased after treatment (4.68±0.22 vs 0.21±0.12, P<0.01), while the methylation level of AGS cells decreased significantly (\[10.1±0.21\]% vs \[90.2±0.67\]%, P<0.01), and the methylation sites in CpG islands were significantly reduced. The degree of HHIP methylation showed a negative correlation with the mRNA expression(r=-0.693, P=0.00). Conclusion: High methylation level of CpG island in HHIP gene promoter may increase the expression of HHIP gene, and then be involved in the carcinogenesis of gastric cancer.


Shen D.,First Peoples Hospital of Zhangjiagang | Zuo Y.,First Peoples Hospital of Zhangjiagang
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To evaluate the efficacy and toxicity of three-dimensional conformal radiotherapy combined with FOLFOX4 regimen in the treatment of advanced unresectable rectal cancer. METHODS: A total of 63 elderly patients with inoperable advanced cancer were retrospectively analyzed, 33 patients in the treatment group received three-dimensional conformal radiotherapy combined with FOLFOX4 regimen (oxaliplatin + CF + 5-FU), and 30 patients only received chemotherapy with FOLFOX4 regimen in the control group. Radiotherapy regimen was 6 MV/15 MV X ray, the radiation dose ranged from 46 to 50 Gy, 2.0 Gy/f. RESULTS: The response rate in the treatment group was 87.8%, significantly higher than that (63.3%) in the control group (P<0.05). The life quality of treatment group was improved better than that of control group, but there was no significant difference (P>0.05). The main side effects were neutropenia, hand and foot numbness, nausea, vomiting and radiation proctitis, there were no significant difference in the two groups (P>0.05). CONCLUSION: The three-dimensional conformal radiotherapy combined with FOLFOX4 regimen are safe and effective in the treatment of elderly patients with advanced cancer, it can be considered as one of the effective treatment for elderly colorectal cancer.


Wang R.,First Peoples Hospital of Zhangjiagang | Chen J.-X.,First Peoples Hospital of Zhangjiagang | Yang W.,First Peoples Hospital of Zhangjiagang
Journal of Practical Oncology | Year: 2010

Objective: To examine the diagnostic value of multi-spiral CT (MSCT) for gastric stromal tumors (GST). Methods: Twenty-five patients with GST confirmed by pathology and immunohistochemistry were included in the study. Both plain and enhanced CT findings were retrospectively analyzed. Results: All the tumors were solitary lesion presenting homogeneous or inhomogeneous density with necrosis, cystis formation and calcification. The types of lesions were intraluminal (n = 4), exophytic pattern (n = 14) or dumbbell pattern (n = 7). Nineteen benign cases with diameter was < 5 cm, presented uniform density and homogenous enhancement; 6 maglinant cases with diameter > 5 cm presented uneven density, heterogenous enhancement, necrosis and cystic degeneration. The extend of enhancement in venous phase was higher than that of arterial phase. One case showed intratumor punctuate calcification. Conclusion: Multi-spiral CT is of value for early diagnosis of GST.

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