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Pang X.-M.,Guangxi Medical University | Liu J.-L.,Guangxi Medical University | Li J.-P.,Guangxi Medical University | Huang L.-G.,Guangxi Medical University | And 6 more authors.
Journal of Neurochemistry | Year: 2015

Previous studies have shown that fastigial nucleus stimulation (FNS) reduces tissue damage resulting from focal cerebral ischemia. Although the mechanisms of neuroprotection induced by FNS are not entirely understood, important data have been presented in the past two decades. MicroRNAs (miRNAs) are a newly discovered group of non-coding small RNA molecules that negatively regulate target gene expression and are involved in the regulation of cell proliferation and cell apoptosis. To date, no studies have demonstrated whether miRNAs can serve as mediators of the brain's response to FNS, which leads to endogenous neuroprotection. Therefore, this study investigated the profiles of FNS-mediated miRNAs. Using a combination of deep sequencing and microarray with computational analysis, we identified a novel miRNA in the rat ischemic cortex after 1 h of FNS. This novel miRNA (PC-3p-3469-406), herein referred to as rno-miR-676-1, was upregulated in rats with cerebral ischemia after FNS. In vivo observations indicate that this novel miRNA may have antiapoptotic effects and contribute to neuroprotection induced by FNS. Our study provides a better understanding of neuroprotection induced by FNS. MicroRNA (miRNA) is defined as a small non-coding RNA that fulfills both the expression and biogenesis criteria. Here, we describe a novel miRNA in the rat ischemic cortex expressed after 1 h of fastigial nucleus stimulation (FNS). The miRNA was functionally characterized by secondary structure, quantitative expression, the conservation analysis, target gene analysis, and biological functions. We consider rno-miR-676-1 to be a true microRNA and present evidence for its neuroprotective effects exerted after induction by FNS. MicroRNA (miRNA) is defined as a small non-coding RNA that fulfills both the expression and biogenesis criteria. Here, we describe a novel miRNA in the rat ischemic cortex expressed after 1 h of fastigial nucleus stimulation (FNS). The miRNA was functionally characterized by secondary structure, quantitative expression, the conservation analysis, target gene analysis, and biological functions. We consider rno-miR-676-1 to be a true microRNA and present evidence for its neuroprotective effects exerted after induction by FNS. © 2015 International Society for Neurochemistry. Source


Li Q.,First Peoples Hospital of Yichang | Chen J.,Huazhong University of Science and Technology | Chen Y.,Huazhong University of Science and Technology | Cong X.,Huazhong University of Science and Technology | Chen Z.,Huazhong University of Science and Technology
Molecular Medicine Reports | Year: 2016

In the present study, pathological alterations in neurons of the dorsal root ganglia (DRG) were investigated in a rat model of chronic sciatic nerve compression. The rat model of chronic sciatic nerve compression was established by placing a 1 cm Silastic tube around the right sciatic nerve. Histological examination was performed via Masson's trichrome staining. DRG injury was assessed using Fluoro Ruby (FR) or Fluoro Gold (FG). The expression levels of target genes were examined using reverse transcription-quantitative polymerase chain reaction, western blot and immunohistochemical analyses. At 3 weeks post-compression, collagen fiber accumulation was observed in the ipsilateral area and, at 8 weeks, excessive collagen formation with muscle atrophy was observed. The collagen volume fraction gradually and significantly increased following sciatic nerve compression. In the model rats, the numbers of FR-labeled DRG neurons were significantly higher, relative to the sham-operated group, however, the numbers of FG-labeled neurons were similar. In the ipsilateral DRG neurons of the model group, the levels of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) were elevated and, surrounding the neurons, the levels of collagen type I were increased, compared with those in the contralateral DRG. In the ipsilateral DRG, chronic nerve compression was associated with significantly higher levels of phosphorylated (p)-extracellular signal-regulated kinase 1/2, and significantly lower levels of p-c-Jun N-terminal kinase and p-p38, compared with those in the contralateral DRGs. Chronic sciatic nerve compression likely induced DRG pathology by upregulating the expression levels of TGF-β1, CTGF and collagen type I, with involvement of the mitogen-activated protein kinase signaling pathway. Source


Wu J.-f.,China Three Gorges University | Feng G.,First Peoples Hospital of Yichang | Shi H.,China Three Gorges University | Zhang Q.-j.,China Three Gorges University | Wang X.-l.,China Three Gorges University
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Studies show that the microencapsulated heterogeneous olfactory bulb cells can reduce the immunological rejection and improve the functional recovery of spinal cord injury. However, the mechanism is unclear. OBJECTIVE: To investigate the effects of the xenotransplantation of microencapsulated olfactory bulb cells into rats on the expression and activity of nuclear factor-kappa B (NF-κB) after spinal cord injury. METHODS: Rabbits were used to prepare the heterogeneous olfactory bulb cell suspension. Sprague Dawley rats were randomly divided into four groups: the sham operation group, the microcapsule group, the cell group and the simple injury group. The rats in the later three groups were prepared for spinal cord hemisection model and transplanted with gelatin sponge sticking 10 μL microencapsulated olfactory bulb cell suspension, 10 μL microencapsulated olfactory bulb cell suspension, and 10 μL physiological saline respectively. The pathological changes of spinal tissues were observed by haematoxylin-eosin staining, and the expressions of NF-κB were observed by immunohistochemical staining. RESULTS AND CONCLUSION: The expressions of NF-κB in the cytoplasm and cytoblast of neurons were increased in rats after spinal cord injury. The expression level reached a peak at 24 hours, gradually decreased after 3 days, and returned to the normal level after 7 days. The NF-κB+ cells in the microcapsule group were obviously fewer than that in the cell and simple injury groups (P < 0.05). The xenotransplantation of microencapsulated olfactory bulb cells can inhibit the expression and activity of NF-κB to mitigate the NF-κB modified inflammatory reaction after spinal cord injury. Source


Han L.,First Peoples Hospital of Yichang | Zhang B.,First Peoples Hospital of Yichang | Li H.,First Peoples Hospital of Yichang | Zuo M.,First Peoples Hospital of Yichang | And 2 more authors.
Chinese-German Journal of Clinical Oncology | Year: 2010

Objective: The paper aimed to study the relationship between the expressions of immunoglobulin G (IgG) subclasses toward human papillomavirus 16-like particles (HPV16VLPs) in the serum of patients and different grades of cervical lesions. Methods: The expressions of IgG subclasses in 32 cases of human papillomavirus (HPV) infection, 30 cervical intraepithelial neoplasia (CIN I), 43 CIN II-III, and 24 hysteromyoma and chronic cervicitis were examined by ELISA. Results: The absorbance values of HPV16VLPs-IgG, IgG1 increased with the grade of CIN (P < 0.05). The IgG2 dominance (IgG2/ IgG1 ratio > 1) from control group was 100%, 87.50% for HPV infection group, 75% for CIN I group, compared with that from CIN II-III patients (9.52%) (P < 0.05). The positive rates and absorbance values of HPV16VLPs-IgG, IgG1 and IgG2 from HPV16-DNA positive group were significantly higher than those from non-HPV16-DNA positive group (P < 0.05). There was a moderate correlation between the HPV16-DNA testing and detection of HPV16VLPs-IgG (r = 0.531, P < 0.05). Conclusion: An increase of the expressions of HPV16VLPs-IgG and its subclasses in the serum of the patients with cervical precancerous lesions, especially those with CIN II-III, might be associated with duration of HPV infection and severity of cervical lesions. An increase of the IgG2 dominance (IgG2/IgG1 > 1) in serum from low grade cervical lesions group and normal control group, might indicate the clearance of HPV infection and the regression of cervical lesions. © Springer-Verlag Berlin Heidelberg 2010. Source


Yao Y.-B.,First Peoples Hospital of Yichang | Wang L.,First Peoples Hospital of Jingmen | Xia Y.,First Peoples Hospital of Yichang | Wang Z.,Shanghai University
Chinese Journal of New Drugs | Year: 2010

Drug castration therapy is important in the treatment of advanced and metastatic prostate cancer. Degarelix is a new potent GnRH-receptor antagonist, which has high water solubility, low histamine release, low systemically allergic reactions and no testosterone surge. In this article, we introduced its features of molecule construction, mechanism of action, pharmacokinetics, and clinical efficacy and safety. Source

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