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Chen H.,Yibin University | Lin Z.,Yibin University | Wu H.,First Peoples Hospital of Yibin | Wang L.,Yibin University | And 2 more authors.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2015

Near-infrared (NIR) spectroscopy has such advantages as being noninvasive, fast, relatively inexpensive, and no risk of ionizing radiation. Differences in the NIR signals can reflect many physiological changes, which are in turn associated with such factors as vascularization, cellularity, oxygen consumption, or remodeling. NIR spectral differences between colorectal cancer and healthy tissues were investigated. A Fourier transform NIR spectroscopy instrument equipped with a fiber-optic probe was used to mimic in situ clinical measurements. A total of 186 spectra were collected and then underwent the preprocessing of standard normalize variate (SNV) for removing unwanted background variances. All the specimen and spots used for spectral collection were confirmed staining and examination by an experienced pathologist so as to ensure the representative of the pathology. Principal component analysis (PCA) was used to uncover the possible clustering. Several methods including random forest (RF), partial least squares-discriminant analysis (PLSDA), K-nearest neighbor and classification and regression tree (CART) were used to extract spectral features and to construct the diagnostic models. By comparison, it reveals that, even if no obvious difference of misclassified ratio (MCR) was observed between these models, RF is preferable since it is quicker, more convenient and insensitive to over-fitting. The results indicate that NIR spectroscopy coupled with RF model can serve as a potential tool for discriminating the colorectal cancer tissues from normal ones. © 2014 Elsevier B.V. All rights reserved.

Zhou F.,University of Sichuan | He M.M.,Guangzhou Medical College | Liu Z.F.,First Peoples Hospital of Yibin | Zhang L.,Peking Union Medical College | And 2 more authors.
Placenta | Year: 2013

Objective: Intrahepatic cholestasis of pregnancy (ICP) is the most common pregnancy-specific liver disorders. Although various biological effects of corticotrophin-releasing hormone (CRH) has in pregnancy have been reported, its activities in patients with ICP are lacking. Here we evaluated CRH and its receptor (CRH-R1) expression in placenta and serum in control and ICP patients, to assess their potential activities in the ICP pathogenesis. Methods and materials: Placental tissues were obtained from the control and ICP patients (10 cases for each group) between 37 and 39 gestational weeks. Immunohistochemistry, Western Blotting and real-time PCR analysis were used to detect the CRH and CRH-R1 expression in placenta. Meanwhile, maternal serums were analyzed for detecting CRH in the control and ICP patients (80 cases for each group) in 34-37 gestational weeks. All data were observed and recorded for comparing and analyzing in control and ICP patients. Results: CRH staining was found in syncytiotrophoblast and feto-placental vascular endothelium cells of placenta, whereas CRH-R1 staining was found in syncytiotrophoblast by using immunohistochemical analysis. The CRH expression level in ICP placenta was significantly lower than those results in controls (P < 0.01). For CRH-R1, CRH mRNA and CRH-R1 mRNA expressions, no statistical differences were found between control and ICP groups (all P > 0.05). Serum CRH levels increased in both control and ICP groups, but the growth rate was limited in ICP group, especially in late pregnancy (P < 0.05). Conclusions: The down-regulation of CRH in ICP placentas and the limited growth rate of CRH in the maternal serum of ICP patients might impair the blood flow regulation of the utero-placental- fetal unit, which might result in poor fetoplacental vascular perfusion and adverse pregnancy outcomes. CRH might play a significant role in the pathogenesis of ICP and provide a new approach to further investigate the etiology of ICP. ©2013 Elsevier Ltd. All rights reserved.

Li J.,University of Sichuan | Li J.,First Peoples Hospital of Yibin | Mao H.,First Peoples Hospital of Yibin | Liang Y.,University of Sichuan | And 4 more authors.
Clinical and Developmental Immunology | Year: 2013

All randomized controlled trials (RCTs) of iguratimod for rheumatoid arthritis (RA) to assess its efficacy and safety are included in this paper. The Review Manager software was used for meta-analysis to assess risk bias of the studies included, and GRADE profiler software was used for the evidence quality of the studies included. Four RCTs involving 1407 patients with RA were included. Meta-analyses showed that, after 24-week therapy, ACR20, tender joint count, swollen joint count, rest pain, physician and patient global assessment of disease activity, HAQ score, ESR, and CRP in iguratimod group were better than those in placebo group and that the difference between those of iguratimod group and those of other DMARDs (MTX and SASP) group was not significant. GRADE evidence classification of the studies included was moderate. Iguratimod for RA had few adverse events, and its efficacy and safety were the same as those of MTX and SASP for RA. The results of this systematic review suggest that more high-quality and large-scaled RCTs were needed to determine the efficacy of iguratimod for RA and whether iguratimod is as effective as other DMARDs besides MTX and SASP. © 2013 Jiangtao Li et al.

Xie M.,Guangxi Medical University | Yi X.,Guangxi Medical University | Wang R.,First Peoples Hospital of Yibin | Wang L.,Guangxi University | And 7 more authors.
Cellular Physiology and Biochemistry | Year: 2014

Background/Aims: Nasopharyngeal carcinoma (NPC) is a distinctive type of head and neck cancer with the highest incidence in South China. Previous studies have proved that matrine, a main alkaloid isolated from Sophora flavescens Ait, has antitumor activity against NPC. However, the effect is not so pronounced and the underlying mechanism remains largely unclear. Here we investigated whether 14-thienyl methylene matrine (YYJ18) that was derived from matrine could exert more effective suppression activity on NPC, along with the underlying mechanism. Methods: NPC cell lines CNE1, CNE2 and HONE1 were treated with YYJ18. Cell proliferation and apoptosis were determined by MTT assay and flow cytometry. Activation of mitogen-activated protein kinases (MAPK) and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathways were determined by Western blotting and quantitative RT-PCR. Results: YYJ18 remarkably inhibited proliferation and induced apoptosis of all three NPC cell lines in a dose-dependent manner, especially in CNE2 cells. Furthermore, YYJ18 treatment significantly suppressed phosphorylation of p38 in CNE2 cells, but upregulated phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and Akt. Next, alterations in downstream signaling were found, including activation of BCL2-associated X protein (Bax), caspase-3 and inactivation of B-cell CLL/lymphoma 2 (Bcl-2). Conclusion: We demonstrate the potent inhibitory effects of 14-thienyl methylene matrine on NPC cells for the first time, which could be mediated by modulation of MAPK and PI3K/Akt pathways. © 2014 S. Karger AG, Basel.

Wu X.,Luzhou Medical College | Zhu X.,Luzhou Medical College | Xie M.,First Peoples Hospital Of Yibin
Medical Science Monitor | Year: 2015

Background: Diet is the primary way cadmium (Cd) enters the body in those without occupational exposure and who do not inhabit Cd-polluted regions. Findings on the relationship between dietary Cd exposure and breast cancer (BC) risk have been inconsistent; a meta-analysis has supported this association but 2 recent cohort studies showed inconsistent results. Hence, we performed an updated meta-analysis to re-evaluate the association between dietary Cd exposure and BC risk. Material/Methods: We searched PubMed, Medline, and EMBASE to identify relevant studies published through September 2014. Combined relative risks (RRs) and the corresponding 95% confdence intervals (CIs) were used to assess the association between dietary Cd exposure and BC risk. Results: We identifed 6 studies involving 321 315 participants and 11 978 cases. Our study suggested there was no statistically signifcant positive association between dietary Cd exposure and BC risk, the combined RR and corresponding 95% CI was 1.01 [0.88, 1.14]. The result was not modifed by menopause status, geographic area, or study design. Conclusions: Our study did not fnd a statistically signifcant positive association between dietary Cd exposure and BC risk. It is necessary to investigate this relationship among the high-risk groups and more cohort studies based on diverse populations are needed. © Med Sci Monit, 2015.

Wei L.,First Peoples Hospital of Yibin | Sun J.-Y.,Soochow University of China | Wang Y.,Soochow University of China | Yang X.,Soochow University of China
Orthopedics | Year: 2011

Combined ipsilateral acetabular and femoral neck fractures are the result of high-energy trauma. Satisfactory treatment for this injury pattern remains a challenge, since traditional open reduction and internal fixation (ORIF) is always accompanied by a high prevalence of posttraumatic arthritis and avascular necrosis of the femoral head. Eight of 502 acetabular fractures from 1990 to 2008 were diagnosed with combined ipsilateral femoral neck fracture, in which 5 patients' fractures were associated with hip dislocation. These patients were injured from falls, traffic accidents, or crushing accidents. Radiographs and computed tomography scans were taken to check acetabular and femoral neck fractures. All of the patients underwent surgery using appropriate approaches and techniques. Postoperative radiographs demonstrated anatomic or satisfactory reduction for acetabular fractures as well as excellent or good reduction for femoral neck fractures in all of the patients. Follow-up radiographs showed femoral head necrosis in the 5 patients with femoral head dislocations, but not in the other 3 patients. We have seen few patients with this injury pattern, which makes us unable to detect significant differences between the patients associated with femoral head dislocation and those without femoral head dislocation. But by considering the results of our study and those reported in the literature, we believe that for patients with ipsilateral acetabular and femoral neck fractures without hip dislocation, satisfactory results could be expected after ORIF. But for those cases associated with hip dislocation, alternative methods such as acute THR as primary treatment are worthy of consideration.

He M.-M.,Guangzhou Medical College | Liu Z.-F.,First Peoples Hospital of Yibin | Wang X.-D.,University of Sichuan
Journal of Sichuan University (Medical Science Edition) | Year: 2011

Objective: To investigate the stereological changes of placental lobular villi vessels in patients with intrahepatic cholestasis of pregnancy (ICP). Methods: Stereological analysis on vessels volume and cross-areas of placental samples taken from 10 normal and 10 ICP patients at 37-40 week pregnancy were performed. Results: Compared with normal pregnancy, the ICP patients had 29% reduction in the volume of placental lobular villi vessels [(287. 81±69. 81) mL vs. (203. 63±41. 29) mL]: 21% reduction in the cross-areas [(617119. 5± 158422. 65) μm 2 vs. (485087. 6± 104707. 63) μm 2]; but no change in the numbers [(313. 40±137. 51) vs. (365. 20±96. 73)] of lobular villi vessels (P>0. 05). Similarly, significant decreases in the volume [(131. 98±84. 68) mL vs. (55.98 ±21.08] mL) and cross-areas [(271809. 6± 162354. 35) μm 2 vs. (127576. 2±54897. 44) μm 2) of placental lobular villi vessels with smooth muscles in the ICP patients were also found (P<0. 05). But the volume [(161. 48 ± 75.80) mL vs. (155. 91±30. 52) mL] and cross-areas [(342639. 6± 145717. 51) μm 2 vs. (341849. 3±57043. 88) μm 2] of placental lobular villi vessels without smooth muscle in the ICP patients remained unchanged (P>0. 05). The placental lobular villi vascular volume was negatively correlated (r= -0. 413) with the maternal plasma level of total bile acid (TBA) (P>0. 05), and positively correlated (r=0. 302) with the onset week of ICP (P>0. 05). But the correlations were not statistically significant. Conclusion: Patients with ICP have 29% reduction in placental lobular villi vascular volume, which is mainly a result of reduction of lobular villi vessels with smooth muscles. The placental lobular villi vascular volume is possibly negatively correled with maternal plasma level of TBA, and positively correled with the onset week of ICP.

Huang G.,University of Sichuan | Liu Z.,First Peoples Hospital of Yibin | He M.,Guangzhou Medical College | Wang X.,University of Sichuan
Gynecologic and Obstetric Investigation | Year: 2014

Objective: Intrahepatic cholestasis of pregnancy (ICP) may lead to sudden onset of stillbirth, which most likely is related to uteroplacental insufficiency and dysregulation of the fetal blood supply. The relaxing effect of corticotropin-releasing hormone (CRH) on blood vessels was measured to examine the role of CRH in the pathogenesis of ICP. Methods: Eighty normal pregnant women and 80 ICP patients were divided into four groups of 20 cases, respectively, each based on gestational age from week 34 to 37. Radioimmunoassay was used to measure CRH in plasma samples collected from all of the subjects. Results: Plasma CRH increased markedly from week 34 to 37 in both ICP and healthy patients, but the increase was lower in the ICP group. Plasma CRH was 322 ± 61 pg/ml in mild ICP cases at 37 weeks' compared to 1,066 ± 173 pg/ml in controls (p < 0.05), but only 218 ± 128 pg/ml in severe ICP (p < 0.05). Plasma CRH was significantly lower at all other measured time points in patients with severe ICP. In ICP patients, there was a negative correlation between plasma CRH and total bile acid (TBA). Conclusion: A limited increasing CRH level and negative correlation of CRH with TBA were unveiled in ICP patients. © 2014 S. Karger AG, Basel.

Cao C.,University of Sichuan | Liu S.,First Peoples Hospital of Yibin | Lou S.-F.,Chongqing Medical University | Liu T.,University of Sichuan
European Review for Medical and Pharmacological Sciences | Year: 2014

BACKGROUND AND OBJECTIVES: Many studies have shown that the +252A/G polymorphism in the lymphotoxin-α gene is implicated in susceptibility to non-Hodgkin lymphoma but with considerable variance of results. This study aimed to clarify the overall association between the +252A/G polymorphism in the lymphotoxin-α gene and non-Hodgkin lymphoma (NHL) risk by performing a meta-analysis. MATERIALS AND METHODS: The Pubmed and Embase databases were searched for all studies relating to lymphotoxin-α +252A/G gene polymorphism and NHL risk. Data were retrieved and statistical analyses were performed using the Revman 5.1 and STATA 12.0 software. RESULTS: Fourteen case-control studies with 25,098 subjects were included. There was no significant association between lymphotoxin-α +252A/G gene polymorphism and the risk of NHL in the all-combined analysis (OR = 1.08, 95%CI: 0.98-1.19 for GG+GA vs. AA; OR = 1.05, 95%CI: 0.95-1.25 for GG vs. GA+AA). In a subgroup analysis by ethnicity, increased NHL risk was found in North Americans (OR = 1.21, 95%CI: 1.05-1.39 for GG+GA vs. AA), no significant association with NHL risk was identified in Asians or Europeans; In a subgroup analysis by NHL subtype, a significantly increased risk was identified in diffuse large B cell lymphoma patients (OR = 1.20 95%CI: 1.11-1.29 for GG+GA vs. AA), but not for follicular lymphoma. CONCLUSIONS: This meta-analysis suggested that the lymphotoxin-α +252A/G gene polymorphism is a risk factor for NHL in North Americans, and this polymorphism may contribute to diffuse large B cell lymphoma susceptibility. Future studies that include different types of NHL and ethnicities are needed to support and extend these observations.

Li T.,First Peoples Hospital of Yibin
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2012

To discuss the improved method and effectiveness of posterior pedicle-screw fixation combined with restoring and grafting through the injured vertebrae for treating thoracolumbar burst fracture. Between March 2008 and September 2010, 21 patients with thoracolumbar burst fracture were treated by posterior pedicle-screw fixation combined with restoring and grafting through the injured vertebrae. Of 21 cases, 15 were male and 6 were female with an age range of 20-61 years (mean, 38.4 years). Affected segments included T12 in 5 cases, L1 in 7 cases, L2 in 5 cases, and T12-L1 in 4 cases. According to Frankel classification for neurological function, 2 cases were rated as grade A, 4 cases as grade B, 6 cases as grade C, 5 cases as grade D, and 4 cases as grade E; based on Denis classification, all 21 cases were burst fractures, including 7 cases of type A, 11 cases of type B, and 3 cases of type C. The X-ray film was taken to measure the relative height of fractured vertebrae and Cobb's angle, and the function of the spinal cord was evaluated at preoperation, postoperation, and last follow-up. All the incisions healed primarily. The 21 patients were followed up 12-30 months (mean, 26 months). No loosening or breakage of screws and rods occurred. X-ray films showed good bone healing with the healing time from 12 to 23 months (mean, 16 months). The Cobb's angles at 1 week and 1 year postoperatively were (3.4 +/- 2.4) degrees and (5.2 +/- 3.2) degrees respectively, showing significant differences when compared with preoperative angle (22.1 +/- 1.2) degrees (P < 0.05), while no significant difference between 1 week and 1 year after operation (P > 0.05). The anterior height of injured vertebrae recovered from (14.6 +/- 2.1) mm (40.2% +/- 1.5% of the normal) at preoperation to (36.0 +/- 2.0) mm (95.3% +/- 1.3% of the normal) at 1 week, and to (35.0 +/- 2.4) mm (94.4% +/- 2.5% of the normal) at 1 year; significant differences were found between preoperation and postoperation (P < 0.05), while no significant difference between 1 week and 1 year after operation (P > 0.05). At 1 year after operation, the Frankel neurological function grade was improved in varying degrees, showing significant difference when compared with preoperative grade (chi2 = 11.140, P = 0.025). Improved method of posterior pedicle-screw fixation combined with restoring and grafting through the injured vertebrae in treatment of thoracolumbar burst fracture can reconstruct the anterior and middle column stability and prevent loss of Cobb's angle and height of vertebrae.

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