First Peoples Hospital of Yancheng
First Peoples Hospital of Yancheng
Huang Z.-J.,First Peoples Hospital Of Yancheng |
Zhu J.-J.,Shanghai University |
Yang X.-Y.,Shanghai University |
Biskup E.,University of Basel
Oncology Letters | Year: 2017
The novel E3 ubiquitin-protein ligase neural precursor cell-expressed developmentally downregulated protein 4 (NEDD4) has been implicated as a crucial factor promoting the tumorigenesis of several types of cancer. The present study investigated the oncogenic role of NEDD4 in hepatocellular carcinoma (HCC) by targeted small interfering RNA silencing of the tumor suppressor phosphatase and tensin homolog (PTEN). Using normal hepatocyte and HCC cell lines, the influence of NEDD4 depletion on proliferation and migration as well as on the PTEN/phosphatidylinositol-3-kinase/protein kinase B signaling pathway was assessed. Additionally, the expression of NEDD4 was assessed in HCC specimens from 78 patients. The in vitro immunohistochemistry results indicated that NEDD4 protein expression was higher, but PTEN expression was lower, in HCC cells compared with normal hepatocytes. The results from the MTT assay, wound healing experiment and Transwell assays demonstrated that NEDD4 depletion lead to decreased proliferation and migration ability of HCC cells. Results from western blotting and immunofluorescence demonstrated that silencing of NEDD4 disrupted the PTEN/phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway in HCC cells. A total of 55 (70.5%) of the HCC specimens stained positive for NEDD4 and expression significantly correlated with tumor size (P=0.047), differentiation degree (P=0.032), vascular invasion (P<0.001), and lymph node metastasis (P=0.005). Thus, NEDD4 appears to perform a critical role in promoting the proliferation and metastasis of HCC via activation of the PTEN/PI3K/AKT signaling pathway; as such, NEDD4 may be a promising target for novel treatments of HCC. © 2017, Spandidos Publications. All rights reserved.
Liu Z.,Xingtai Peoples Hospital |
Qi L.,Taian Central Hospital |
Zhu J.,First Peoples Hospital Of Yancheng
Biomedical Research (India) | Year: 2017
Objective: To explore the feasibility and clinic value of super-early operation in treatment of high-grade intracranial aneurysm with ruptured hemorrhage. Methods: From August, 2014 to March, 2016, 46 patients suffering high-grade ruptured intracranial aneurysm were collected. Among them, 23 patients were treated with early operation (operation within 48 or 72 h, group A) while the rest received super-early operation (operation within 24, group B). The differences of effect, post-operation complication, and recovery between groups A and group B were analysed statistically with chi-squire test. Results: Compare to the 30.43% aneurysms rupture recurrence rate in patients who received early operation (group A), the recurrence rate of aneurysms rupture in patients receiving super-early operation (group B) is lower to 4.35%. Additionally, the post-operation complication rate of group B (8.69%) is significantly lower than that of group A (39.13%). Consistently, 18 out 23 patients in group B recovered very well from the operation, while only 10 out 23 patients in group achieved good recovery. Conclusion: Super-early operation has certain clinical application value in treatment of high-grade ruptured intracranial aneurysm. © 2017, Scientific Publishers of India. All rights reserved.
Wang Y.-L.,Nanjing Medical University |
Li F.,First Peoples Hospital of Yancheng |
Chen X.,Nanjing Medical University
European Journal of Pharmacology | Year: 2016
Postnatal propofol exposure impairs hippocampal synaptic development and memory. However, the effective agent to alleviate the impairments was not verified. In this study, piracetam, a positive allosteric modulator of AMPA receptor was administered following a seven-day propofol regime. Two months after propofol administration, hippocampal long-term potentiation (LTP) and long-term memory decreased, while intraperitoneal injection of piracetam at doses of 100 mg/kg and 50 mg/kg following last propofol exposure reversed the impairments of memory and LTP. Mechanically, piracetam reversed propofol exposure-induced decrease of BDNF and phosphorylation of mTor. Similar as piracetam, BDNF supplementary also ameliorated propofol-induced abnormalities of synaptic plasticity-related protein expressions, hippocampal LTP and long-term memory. These results suggest that piracetam prevents detrimental effects of propofol, likely via activating BDNF synthesis. © 2016 Elsevier B.V. All rights reserved.
Sun J.,First Peoples Hospital of Yancheng
Molecular Medicine Reports | Year: 2013
Single nucleotide polymorphisms (SNPs) of Xeroderma pigmentosum group D (XPD) are associated with various types of cancer. However, previous studies of correlations between SNPs in this gene and esophageal squamous cell carcinoma (ESCC) have generated conflicting results. In the present study, we investigated the potential relationship between SNPs in two key regions of XPD, codons 312 and 751 and ESCC in a Chinese population. Polymerase chain reaction-restriction fragment length polymorphism was used to analyze genotypes at codons 312 and 751 of XPD in 400 ESCC patients (case group) and 400 healthy individuals (control group). Logistic regression was used to analyze the relationship between genotypes and ESCC. No statistically significant difference was observed for the genotype or allele frequencies of codon 312 between case and control groups (P>0.05). However, a statistically significant difference was observed in the genotype and allele frequencies of codon 751 between the case and control groups (P<0.05). Specifically, compared with the AA genotype at codon 751, a significant increase in risk of ESCC was detected for individuals with the CC genotype (OR=1.600; 95% CI, 1.137-2.253; P=0.007). Therefore, XPD polymorphism at codon 312 is not correlated with ESCC, while polymorphism at codon 751 is associated with ESCC and the CC genotype may confer increased susceptibility to the disease. Copyright © 2013 Spandidos Publications Ltd.
Wu J.,First Peoples Hospital of Yancheng |
Zhang J.-R.,Peoples Hospital of Dafeng |
Jiang X.-Q.,Peoples Hospital of Dafeng |
Cao X.-G.,First Peoples Hospital of Yancheng
Molecular Medicine Reports | Year: 2017
The aim of the present study was to investigate the association between the expression level of secreted protein acidic and rich in cysteine (SPARC) and the prognosis of postoperative patients with esophageal squamous cell carcinoma (ESCC). The expression level of SPARC was detected in the 89 ESCC tissue cases and 100 healthy esophageal mucosa cases, which served as the controls. Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were employed to evaluate the SPARC expression in cases with ESCC. RT-PCR demonstrated that the positive rates of SPARC mRNA expression in ESCC were 71.91% (64/89). The positive rates of normal esophageal mucosa mRNA expression were 15.00% (15/100), which were significantly lower than that in the ESCC tissue samples. The difference was statistically significant (P<0.001). Immunohistochemical staining indicated that the positive expression rate of SPARC protein in the ESCC tissue samples was significantly higher than that in the esophageal mucosa tissue samples (65.17 vs. 8.00%; P<0.001). The expression of SPARC protein was negatively correlated with lymph node metastasis (P<0.05), which was not associated with the pathologic gross morphology, tumor differentiation degree or other clinical features. The survival of patients with ESCC was not associated with the expression level of SPARC protein (P>0.05), but was associated with the tumor location (P<0.05), differentiation (P<0.001) and staging (P<0.05). Thus, SPARC mRNA and protein were highly expressed in ESCC, and negatively correlated with lymph node metastasis, which was not associated with postoperative survival of ESCC patients. Thus, detection of SPARC mRNA and protein expression levels may facilitate early diagnosis and prognosis assessment of ESCC.
Xu L.,First Peoples Hospital of Yancheng |
Huang S.,Sun Yat Sen University |
Chen W.,Sun Yat Sen University |
Song Z.,Wenzhou University |
Cai S.,Second Hospital of Yancheng
Tumor Biology | Year: 2014
Previous studies on the associations of the NFKB1 -94 insertion/deletion polymorphism with cancer risk have produced conflicting results. The purpose of this meta-analysis is to define the effect of the NFKB1 -94 insertion/deletion polymorphism on cancer risk. A search of the literature by PubMed was performed to identify studies based on the predetermined inclusion criteria. Twenty-three studies consisting of 6,494 cases and 9,884 controls were identified and analyzed. Overall, significant association was observed between the polymorphism and cancer risk under all genetic models. Subgroup analysis according to ethnicity and cancer type also detected significant association. The NFKB1 -94 insertion/deletion polymorphism was associated with cancer risk in Asian population (dominant model: OR=1.52, 95 % CI=1.17-1.98; recessive model: OR=1.50, 95 % CI=1.26-1.79; II vs. DD: OR=1.90, 95 % CI=1.37-2.65; ID vs. DD: OR=1.32, 95 % CI=1.05-1.66; I vs. D: OR=1.37, 95 % CI=1.17-1.60), but not in Caucasian population. In addition, significant associations in OC, HCC, and OSCC were observed, but significant associations were not found in BC and LC. The current meta-analysis suggested that NFKB1 -94 insertion/deletion polymorphism may influence cancer risk in Asian population. © 2014 International Society of Oncology and BioMarkers (ISOBM).
Zheng X.-Z.,First Peoples Hospital of Yancheng |
Zheng X.-Z.,Nanjing University |
Zheng Q.,First Peoples Hospital of Yancheng |
Zhou J.,Third Peoples Hospital of Yancheng |
Yang B.,Nanjing University
Journal of Ultrasound in Medicine | Year: 2015
Objectives-This study was conducted to evaluate the value of sonographic B-lines (previously called "comet tail artifacts") in assessment of pulmonary hypertension in patients with interstitial lung diseases. Methods-One hundred thirty-four patients with clinically diagnosed interstitial lung diseases complicated by pulmonary hypertension underwent transthoracic lung sonography and Doppler echocardiography for assessment of the presence of B-lines, the distance between them, and the pulmonary artery (PA) systolic pressure. A correlation analysis and a receiver operating characteristic curve analysis were performed. Results-All patients had diffuse bilateral B-lines. The maximum number of B-lines seen in any positive zone (not a summation) was significantly correlated with the severity of PA systolic pressure (r = 0.812; P< .0001), and a linear regression equation could be demonstrated: that is, y = 6.06 x + 17.57, where x and y represent the number of Blines and PA systolic pressure, respectively. A cutoff of more than 4 B-lines seen in any positive zone had 89.5% sensitivity, 85.0% specificity, and 87.2% accuracy in predicting elevated PA pressure (>30 mm Hg). Conclusions-The number of B-lines is useful in assessment of pulmonary hypertension, especially when tricuspid regurgitation and pulmonary valve regurgitation do not exist or cannot be satisfactorily measured by Doppler echocardiography. © 2015 by the American Institute of Ultrasound in Medicine.
Chen P.,First Peoples Hospital of Yancheng |
Zhao X.,First Peoples Hospital of Yancheng |
Ma L.,First Peoples Hospital of Yancheng
Molecular and Cellular Biochemistry | Year: 2013
Increasing evidence suggests that dysregulation of microRNAs is correlated with malignant transformation and tumor development. miR-100, a potential tumor suppressor, is downregulated by many human cancers. However, the expression and functions of miR-100 in hepatocellular carcinoma (HCC) are still unclear. The aim of this study was to detect the expression of miR-100 in HCC tissues and investigate its clinicopathological and prognostic significance. Also, the effects of miR-100 on growth and apoptosis of HCC cells and its potential molecular mechanisms were analyzed. Results showed that the expression level of miR-100 in HCC tissues was significantly lower than that in matched non-cancerous liver tissues. Also, low-miR-100 expression was observed to be significantly correlated with higher tumor grade, higher incidence of lymph node metastasis, advanced TNM stage and higher incidence of tumor recurrence in HCC patients. Multivariate survival analyses suggested that low-miR-100 expression was an independent prognostic factor for HCC patients (HR = 1.66, 95 % CI 1.32-2.82, P = 0.019). In addition, we found that upregulation of miR-100 could inhibit growth and increase apoptosis of HCC cells by downregulating polo-like kinase 1 (plk1). In HCC tissues, miR-100 expression was inversely correlated with the expression of plk1 protein (r = -0.418; P = 0.029). Therefore, downregulation of miR-100 was correlated with progressive pathological feature and poor prognosis in HCC patients, and miR-100 could function as a tumor suppressor by targeting plk1. miR-100 may serve as a prognostic marker and molecular therapeutic target in HCC. © 2013 Springer Science+Business Media New York.
PubMed | First Peoples Hospital of Yancheng
Type: Journal Article | Journal: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine | Year: 2015
The clinical presentation of myocarditis often mimics acute coronary syndrome. Coronary sinus flow has been used for detection of the presence of myocardial ischemia. Whether myocarditis is associated with changes in coronary sinus flow remains unknown. The aim of this study was to assess coronary sinus flow at the onset and follow-up of myocarditis mimicking acute coronary syndrome using transthoracic Doppler echocardiography (TTE).Sixty-four patients with clinically diagnosed viral myocarditis mimicking acute coronary syndrome underwent TTE on days 3, 7, 30, 90, 180, and 360 after onset. Coronary sinus flow was compared among different points in time.Compared to healthy participants, all patients with myocarditis had a larger cardiac size, reduced cardiac function, and electrocardiographic and myocardial enzyme abnormalities on days 3 and 7 days (P< .01; P< .05). They later had gradual restoration to normal levels. On days 3 and 7, the coronary sinus flow in patients with myocarditis was extremely lower than that in healthy participants (about one-tenth), although coronary angiography revealed unobstructed arteries. On days 30, 90, 180, and 360, the coronary sinus flow had been increasing; however, it was still far less than that in healthy participants (P < .01).Coronary sinus flow depicted by TTE is reduced but recovers with time in viral myocarditis mimicking acute coronary syndrome, which is a useful indicator in the follow-up of this type of myocarditis.
PubMed | University of California at San Diego, First Peoples Hospital of Yancheng, China Japan Friendship Hospital and Soochow University of China
Type: Journal Article | Journal: Oncology letters | Year: 2016
Dobutamine has been widely used for the treatment of heart failure and cardiogenic shock since the 1970s. Osteosarcoma is the most commonly observed malignant bone tumor in children. Currently, there are no effective drugs for the treatment of osteosarcoma. In the present study, the potential anticancer activity of dobutamine on human osteosarcoma cells was examined. Human osteosarcoma MG-63 cells were treated with dobutamine at various concentrations and for various incubation times. The inhibition of cell growth by dobutamine was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Flow cytometry was utilized to evaluate the effect of dobutamine on cell apoptosis and the cell cycle. Furthermore, the expression levels of caspase-3 and caspase-9 were assessed by western blot analysis. The influence of dobutamine on cancer cell migration and invasion was additionally evaluated using wound-healing assay and the Boyden Chamber migration method. Dobutamine significantly inhibited the growth of MG-63 cells at a concentration of 10 M or higher when incubated for 12 h or longer (P=0.023). Dobutamine augmented cell apoptosis and arrested the cell cycle in the G2/M phase. Western blot analysis revealed that dobutamine induces expression of caspase-3 and caspase-9. In addition, the invasiveness and migration of MG-63 cells was inhibited by dobutamine in a concentration-dependent manner. The results of the present study may lead to novel applications for dobutamine in the treatment of osteosarcoma.