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Wu X.-J.,First Peoples Hospital of Xuzhou | Mi Y.-Y.,Xuzhou Medical College | Hu A.-K.,Xuzhou Medical College | Li C.,First Peoples Hospital of Xuzhou | And 2 more authors.
Onkologie(Czech Republic) | Year: 2013

Background: Single-nucleotide polymorphisms (SNPs) in microRNAs (miRNA) have been shown to be related with susceptibility to several human cancers. We evaluated the associations of rs3746444 in pre-miRNA hsa-mir-499 with the risk of gastric cancer (GC) in the Chinese population. Patients and Methods: The rs3746444 (A>G) SNPs were genotyped in 201 GC and 213 non-cancer subjects in a case-control study by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Results: There was no significant overall difference in the genotype distributions of rs3746444 (A>G) SNPs between cases and controls. In the logistic regression analyses, no significantly increased risk of GC was found to be associated with variant genotypes. Conclusion: The rs3746444 (A>G) SNP is not associated with susceptibility to GC in the Chinese population. © 2013 S. Karger GmbH, Freiburg.


Ge X.-H.,Soochow University of China | Ge X.-H.,First Peoples Hospital of Xuzhou | Zhu G.-J.,Soochow University of China | Geng D.-Q.,Xuzhou Medical College | And 2 more authors.
Neurological Sciences | Year: 2012

The aim of this study was to determine the mechanism by which erythropoietin (EPO) suppressed 6-hydroxydopamine (6-OHDA)-induced apoptosis. Our results showed that 6-OHDA remarkably decreased phosphorylation of glycogen synthase kinase 3β (GSK3β) as well as enhanced the level of Bax in the mitochondria. Besides, 6-OHDA decreased the mitochondrial expression of Bcl-2 without altering the cytoplasmic expression of Bcl-2. In line with these results, 6-OHDA treatment enhanced the apoptosis and caspase 3 activity in PC12 cells. These findings indicated that mitochondrial dysfunction was involved in the neurotoxicity of 6-OHDA and GSK3β might act upstream of Bax/Bcl-2 and the caspase 3 pathways in 6-OHDA-treated PC12 cells. Furthermore, EPO reduced 6-OHDA-induced growth inhibition. Western blot exhibited that GSK3β inhibitor 4-benzyl-2-methyl-1, 2,4-thiadiazolidine-3, 5-dione (TDZD8) and EPO not only increased the phosphorylation of GSK3β but also inhibited the mitochondrial translocation of Bax. In agreement with these results, EPO and TDZD8 obviously increased the mitochondrial expression of Bcl-2. Finally, TDZD-8 and EPO significantly suppressed the enhanced apoptosis and activity of caspase 3 induced by 6-OHDA. Taken together, GSK3β-mediated mitochondrial cell death pathway is involved in the neuroprotective effect of EPO against 6-OHDA-induced apoptosis. © The Author(s) 2011.


Kong M.,First Peoples Hospital Of Xuzhou | Li B.,First Peoples Hospital Of Xuzhou | Tian Y.,First Peoples Hospital Of Xuzhou
Experimental and Therapeutic Medicine | Year: 2016

The number of elderly patients undergoing femoral head replacement surgeries is on the increase. These patients often suffer from comorbidity such as cardiovascular and cerebrovascular complications, which limits the ability of medical teams to employ anesthesia. Thus, alternative methods are required. The aim of this study was to examine the advantage of laryngeal mask airway (LMA) in the absence of muscle relaxant in elderly patients undergoing femoral head replacement operations. Fifty patients (27 males and 23 females) undergoing femoral head replacements were selected for the study between March 2013 and May 2014. The mean value for the age in this group was 74.6±12.5 years. The patients were randomly distributed into two groups of 25. One group was designated as the treatment group and the second group as the control group. For the treatment group, LMA without muscle relaxant was used, and the control group received routine anesthesia. Variations in heart rate (HR), mean arterial pressure (MAP) and oxygen saturation (SPO2) in the two groups were monitored at different times. Clinical efficacy and muscle relaxation effects were also analyzed. For the treatment group, the HR, MAP and SPO2 measurements did not reveal any significant variation while these values in the control group demonstrated important dissimilarities. Time to recovery, time to extubation and incidence of throat pain in the treatment group were all markedly decreased as compared to those in control group. The operation time in the treatment group was not significantly different to that of control group. The satisfaction of the muscle relaxation effect in the treatment group was significantly higher than that in the control group while the incidence of adverse reactions was not considerably different. In conclusion, the use of LMA without using muscle relaxant in femoral head replacement surgeries performed on elderly patients showed to be effective and safe. © 2016, Spandidos Publications. All rights reserved.


Wu X.,First Peoples Hospital of Xuzhou | Mi Y.,Xuzhou Medical College | Yang H.,First Peoples Hospital of Xuzhou | Hu A.,Xuzhou Medical College | And 2 more authors.
Molecular and Cellular Biochemistry | Year: 2013

Extracellular high-mobility group box-1 (HMGB-1) has been implicated in the inflammation response leading to the precancerous lesions of non-small cell lung cancer (NSCLC). However, the role of HMGB-1 in the inflammation response in normal human bronchial epithelial (NHBE) cells and its underlying mechanisms were still not fully understood. In this study, the inflammation response in NHBE cells was stimulated by 2.5, 5, and 10 μg/ml HMGB-1. However, the receptor for advanced glycation end products (RAGE) blocker RAGE-Ab (5 μg/ml) or 10 μM c-Jun N-terminal kinases (JNK) inhibitor SP600125 could inhibit HMGB1-induced the release of inflammation cytokines including TNF-α, IL-8, IL-10, and MCP-1 in a dose-dependent manner. Furthermore, HMGB1-induced RAGE protein expression, JNK and NF-κB activation were attenuated by the pretreatment with RAGE-Ab or JNK inhibitor SP600125 in Western blot analysis. Our data indicated that HMGB-1 induced inflammation response in NHBE cells through activating RAGE/JNK/NF-κB pathway. HMGB-1 could act as a therapeutic target for inflammation leading NHBE cells to the precancerous lesions of NSCLC. © 2013 Springer Science+Business Media New York.


Huang H.,First Peoples Hospital Of Xuzhou | Zhou H.,First Peoples Hospital Of Xuzhou | Wang N.,First Peoples Hospital Of Xuzhou
Cell Biochemistry and Biophysics | Year: 2015

One of the most common neurological disorders is epilepsy, which, approximately affecting more than 40 million people worldwide. The situation worsens in the affected patient when the epilepsy becomes intractable, that simply means failure of anti-epileptic drugs (AED) to achieve seizure freedom. It is also associated with an increased prevalence of mental health disorders including anxiety, depression, and suicidal thoughts. The present review is focused on the recent advances being utilized world over for the effective management of epilepsy patients. The review article discusses the efficacy and importance of recent AED drugs in use for management of epilepsy on one hand and on other hand highlights the use of modern advancements like High frequency oscillations, Network analysis, Seizure localization, Epilepsy surgery in curing intractable epilepsy. © 2015, Springer Science+Business Media New York.


Fan S.-H.,Jiangsu University | Wang Y.-Y.,First Peoples Hospital of Xuzhou | Lu J.,Jiangsu University | Zheng Y.-L.,Jiangsu University | And 6 more authors.
Journal of Cellular Biochemistry | Year: 2015

Ceramide synthase 2 (CERS2) is the gene identified from a human liver cDNA library in 2001. Our previous studies have shown higher expression of CERS2 in the breast cancer patients was associated with fewer lymph node metastases. However, the molecular mechanism of CERS2 involved is unknown. Here, we found CERS2 was heterogeneously expressed in various breast cancer cells. The mRNA and protein expression levels of CERS2 in MCF7 cells, which are poorly invasive breast cancer cells, were obviously higher than that in the highly invasive cells MDA-MB-231. Results showed overexpression of CERS2 in MDA-MB-231 cells could significantly inhibit the migration and invasion ability, whereas CERS2 knockdown in MCF7 cells could significantly increase the migration and invasion ability. Overexpression of CERS2 in MDA-MB-231 cells significantly reduced the V-ATPase activity, increased the extracellular pH and decreased the pH-dependent activity of MMP-2 and MMP-9 matrix metalloproteinases (MMPs). CERS2 knockdown in MCF7 cells significantly increased the V-ATPase activity, decreased the extracellular pH and increased the activity of MMP-2 and MMP-9. Taken together, CERS2 can significantly inhibit breast cancer cell invasion and is associated with the decrease of the V-ATPase activity and extracellular hydrogen ion concentration, and in turn the activation of secreted MMP-2/MMP-9 and degradation of extracellular matrix (ECM), which ultimately suppressed tumor's invasion. Thus, CERS2 may represent a novel target for selectively disrupting V-ATPase activity and the invasive potential of cancer cells. J. Cell. Biochem. 116: 502-513, 2015. © 2014 Wiley Periodicals, Inc.


Fan S.-H.,Jiangsu University | Wang Y.-Y.,First Peoples Hospital of Xuzhou | Lu J.,Jiangsu University | Zheng Y.-L.,Jiangsu University | And 6 more authors.
PLoS ONE | Year: 2014

The inflammasome is a multi-protein complex which when activated regulates caspase-1 activation and IL-1β secretion. Inflammasome activation is mediated by NLR proteins that respond to stimuli. Among NLRs, NLRP3 senses the widest array of stimuli. NLRP3 inflammasome plays an important role in the development of many cancer types. However, Whether NLRP3 inflammasome plays an important role in the process of hepatocellular carcinoma (HCC) is still unknown. Here, the anticancer effect of luteoloside, a naturally occurring flavonoid isolated from the medicinal plant Gentiana macrophylla, against HCC cells and the underlying mechanisms were investigated. Luteoloside significantly inhibited the proliferation of HCC cells in vitro and in vivo. Live-cell imaging and transwell assays showed that the migration and invasive capacities of HCC cells, which were treated with luteoloside, were significantly inhibited compared with the control cells. The inhibitory effect of luteoloside on metastasis was also observed in vivo in male BALB/c-nu/nu mouse lung metastasis model. Further studies showed that luteoloside could significantly reduce the intracellular reactive oxygen species (ROS) accumulation. The decreased levels of ROS induced by luteoloside was accompanied by decrease in expression of NLRP3 inflammasome resulting in decrease in proteolytic cleavage of caspase-1. Inactivation of caspase-1 by luteoloside resulted in inhibition of IL-1β. Thus, luteoloside exerts its inhibitory effect on proliferation, invasion and metastasis of HCC cells through inhibition of NLRP3 inflammasome. Our results indicate that luteoloside can be a potential therapeutic agent not only as an adjuvant therapy for HCC, but also, in the control and prevention of metastatic HCC. © 2014 Fan et al.


PubMed | First Peoples Hospital of Xuzhou
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

One of the most common neurological disorders is epilepsy, which, approximately affecting more than 40 million people worldwide. The situation worsens in the affected patient when the epilepsy becomes intractable, that simply means failure of anti-epileptic drugs (AED) to achieve seizure freedom. It is also associated with an increased prevalence of mental health disorders including anxiety, depression, and suicidal thoughts. The present review is focused on the recent advances being utilized world over for the effective management of epilepsy patients. The review article discusses the efficacy and importance of recent AED drugs in use for management of epilepsy on one hand and on other hand highlights the use of modern advancements like High frequency oscillations, Network analysis, Seizure localization, Epilepsy surgery in curing intractable epilepsy.


PubMed | First Peoples Hospital of Xuzhou
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016

The number of elderly patients undergoing femoral head replacement surgeries is on the increase. These patients often suffer from comorbidity such as cardiovascular and cerebrovascular complications, which limits the ability of medical teams to employ anesthesia. Thus, alternative methods are required. The aim of this study was to examine the advantage of laryngeal mask airway (LMA) in the absence of muscle relaxant in elderly patients undergoing femoral head replacement operations. Fifty patients (27 males and 23 females) undergoing femoral head replacements were selected for the study between March 2013 and May 2014. The mean value for the age in this group was 74.612.5 years. The patients were randomly distributed into two groups of 25. One group was designated as the treatment group and the second group as the control group. For the treatment group, LMA without muscle relaxant was used, and the control group received routine anesthesia. Variations in heart rate (HR), mean arterial pressure (MAP) and oxygen saturation (SPO


PubMed | Capital Medical University, First Peoples Hospital of Xuzhou, Massachusetts Eye and Ear Infirmary and Beijing Anzhen Hospital
Type: Journal Article | Journal: Eye (London, England) | Year: 2016

PurposeTo report surgical outcomes of microcatheter-assisted trabeculotomy following failed angle surgeries, and compare those with no previous angle surgery, in primary congenital glaucoma (PCG).MethodsThe early postoperative (12 months) results of 42 eyes of 36 patients who underwent microcatheter-assisted trabeculotomy by single surgeon for PCG were retrospectively analyzed. Group 1, 20 eyes of 16 patients, had no previous angle surgery. Group 2, 22 eyes of 20 patients, had one or two previous failed angle surgeries. Success was defined as an intraocular pressure (IOP) <21mmHg with at least a 30% reduction from preoperative IOP with (qualified success) or without (complete success) the use of antiglaucoma medication.ResultsMean IOP decreased from 31.57.2mmHg on 3 (median, range: 1-5) medications in Group 1 and 34.67.3mmHg on 3 (median, range: 1-4) medications in Group 2 preoperatively to 15.63.1mmHg on 0 (median, range: 0-4) medications in Group 1 and 16.04.6mmHg on 0 (median, range: 0-2) medications in Group 2 postoperatively at 12 months (both P<0.001), respectively. The mean percentage of IOP reduction from preoperative to last postoperative visit was 46.020.1% in Group 1 and 45.525.0% in Group 2, P=0.947. Qualified and complete successes were comparable between Group 1 and Group 2 (qualified success: 90.0% vs 77.3%, P=0.294; complete success: 78.9% vs 77.3%, P=0.853). Complications were minimal.ConclusionsMicrocatheter-assisted trabeculotomy achieved significant pressure-lowering effects with a reduction in medication use in PCG, and it represents a reasonable choice of initial and repeat surgical treatment for PCG.

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