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Lin H.,First Peoples Hospital of Wenling | Pan E.-C.,Centers for Disease Control and Prevention | Shan H.-S.,Bengbu Medical College | Wen X.-Y.,Bengbu Medical College | And 4 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2014

OBJECTIVE: To investigate the incidence and distribution characteristics of cancer in Huai'an during the period of 2009-2011 for prevention and control of the disease. METHODS: Based on demography and death data collected through Huai'an's Disease Surveillance System, 2009-2011, the incidence rate, age-standardized rate and cumulative rate as well as the distribution characteristics of cancer in Huai'an were analyzed. RESULTS: The incidence rate and age-standardized rate of cancer were 205.60/105 and 166.22/105, the crude and standardized rates of man were higher than that of women, the incidence gradually increased with age over 40 years old, the highest peak was in the age of 70-75 years old. The top 10 most common cancer sites in Huai'an area during the 3-year period in order were esophagus, stomach, lung (including trachea and bronchus), liver, colon-rectum (including anus), breast, pancreas, cervix uteri, brain and CNS and leukemia, which accounted for 87.56% of all cancers. The incidence rate of esophageal cancer was 62.91/105, accounting for 30.60% of all cancers. It was located in the first onset in male, female, city and rural population. CONCLUSIONS: Cancer is one kind of major diseases threatening people's health in Huai'an area and a major threats to the elderly population. Although the incidence of malignant tumor is lower than that in the national average level, but from a single disease situation, esophageal cancer had a high incidence. Cancer prevention and control should be enhanced, especially for esophageal cancer.


Gong Y.-N.,Chinese National Institute for Communicable Disease Control and Prevention | Gong Y.-N.,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases | Li Y.-M.,Zhejiang University | Yang N.-M.,Zhiyuan Medical Inspection Institute Co. | And 28 more authors.
World Journal of Gastroenterology | Year: 2015

AIM: To evaluate the efficacy of centralized culture and possible influencing factors. METHODS: From January 2010 to July 2012, 66452 patients with suspected Helicobacter pylori (H. pylori ) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy. Gastric mucosal biopsies were taken from the antrum for culture. These biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time: 5, 24 and 48 h. The culture results were reported after 72 h and the positive culture rates were analyzed by a χ 2 test. An additional 5736 biopsies from H. pylori -positive patients (5646 rapid urease test-positive and 90 14C-urease breath test-positive) were also cultured for quality control in the central laboratory setting. RESULTS: The positive culture rate was 31.66% (21036/66452) for the patient samples and 71.72% (4114/5736) for the H. pylori -positive quality control specimens. In the 5 h transport group, the positive culture rate was 30.99% (3865/12471), and 32.84% (14960/45553) in the 24 h transport group. In contrast, the positive culture rate declined significantly in the 48 h transport group (26.25%; P < 0.001). During transportation, the average natural temperature increased from 4.67 to 29.14 ?, while the positive culture rate declined from 36.67% (1462/3987) to 24.12% (1799/7459). When the temperature exceeded 24 °C., the positive culture rate decreased significantly, especially in the 48 h transport group (23.17%). CONCLUSION: Transportation of specimens within 24 h and below 24 °C. is reasonable and acceptable for centralized culture of multicenter H. pylori samples. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Li X.-L.,First Peoples Hospital of Wenling | Zheng Z.-J.,First Peoples Hospital of Wenling | Qu H.-O.,First Peoples Hospital of Wenling
JRAAS - Journal of the Renin-Angiotensin-Aldosterone System | Year: 2015

Objective: Previous case-control studies on the relation between angiotensin-converting enzyme (ACE) gene insertion/ deletion (I/D) polymorphism and breast cancer did not reach the same conclusion. In the present study, we aimed to further evaluate the relationship between the ACE gene I/D polymorphism and breast cancer. Methods: We selected 13 case-control studies related to ACE gene I/D polymorphism and breast cancer by searching PubMed, EMBase, Chinese Biomedical Literature Database, Chinese CNKI, and Wanfang database. To test the heterogeneity between each study, we utilized the Q-test and I2 test. To merge the odds ratio (OR) and 95% confidence interval (CI), we utilized the random effects model during the analyses. Results: The present study included 1997 patients with breast cancer and 8404 cancer-free control subjects. By metaanalysis, we did not find any association of ACE gene I/D polymorphism with breast cancer in different model (DD vs (ID+II): OR=1.28, 95% CI (0.90-1.81), p=0.16; II vs (ID+DD): OR=0.99, 95% CI (0.81-1.21), p=0.93; D allele vs I allele: OR=1.15, 95% CI (0.94-1.41), p=0.18). Conclusion: We concluded that ACE gene I/D polymorphism was not associated with breast cancer. © The Author(s) 2014.


Jiang Z.,Zhejiang University | Jiang Z.,First Peoples Hospital of Wenling | Zhang W.,First Peoples Hospital of Wenling | Lin N.,Zhejiang University | And 2 more authors.
Tumor | Year: 2016

Objective: To explore the clinical manifestations, treatment methods, therapeutic efficacy and outcomes of soft tissue osteosarcoma. Methods: The clinical records and follow-up information of 10 patients with soft tissue osteosarcoma admitted in Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine from January 2002 to December 2014 were retrospectively analyzed. Results: Of 10 patients with soft tissue osteosarcoma, six were male, and four were female; 7 patients had tumors located in limbs, and 3 had tumors located in groin, ilium and hip, respectively; 7 tumor lesions were located in superficial layer of deep fascia, and 3 were located in deep layer; concerning tumor size, the tumor diameter was larger than 10 cm in 4 cases , 5-10 cm in 4 cases, and shorter than 5 cm in 2 cases; regarding to pathologic grading, 9 cases were high-grade osteosarcoma, and 1 case was low to medium grade. All patients underwent tumor resection, of which, 4 cases received post-operative chemotherapy, and radiotherapy was not utilized. Three cases suffered from local recurrence, in which, 4 cases also had distant metastasis; no metastasis alone was observed. Four cases died of lung metastasis. Conclusion: Soft tissue osteosarcoma is characterized by low morbidity, high malignant grade, high local recurrence rate and distant metastasis rate, as well as high mortality. Extensive resection is the most important therapeutic strategy for soft tissue osteosarcoma, and its outcomes are related to resection extent and tumor size. Chemotherapy is not a validated treatment strategy, and radiotherapy is also seldom utilized in treatment of soft tissue osteosarcoma. Copyright © 2016 by TUMOR. All rights reserved.


Xia C.-M.,First Peoples Hospital of Wenling | Xia C.-M.,Fudan University | Zhang S.-C.,Fudan University
Fudan University Journal of Medical Sciences | Year: 2010

Objective To investigate the effect of Schistosoma ja ponicum ova on the expression of intestinal NOD2/CARD15 in the mice induced by 2,4,6- trinitrobenzesulfonic acid (TNBS). Methods Mice (n = 50) in the experiment were randomly allocated into 3 groups: control group (n = 10) , TNBS+ saline (n = 20) and TNBS+ Schistosoma japonicum ova (n = 20). TNBS enema (100 mg/kg) was applied to the two TNBS groups in order to establish a colonitis model. Schistosoma japonicum ova was administered i. p. on the 14th and 3rd day before the instillation of haptenating agent. All mice were killed on the 7th day after colitis induction. The transcription level of NOD2 in colon tissues was measured by Real time PCR, and the expression of NOD2 protein was measured by Western blot. Results The transcription and protein levels of NOD2 in TNBS-induced mice increased statistically compared with those of the normal group (P<0. 05). Compared with the TNBS-induced mice, Schistosoma japonicum ova-treated ones exhibited a statistical reduction of gene and protein expression (P < 0. 05). Conclusions TNBS-treated mice exhibited a statistical increased expression of NOD2/CARD15, Schistosoma japonicum ova treatment reduced the severity of experimental colitis through down-regulating NOD2/CARD15.


Dai D.-P.,Beijing Hospital | Hu L.-M.,First Peoples Hospital of Wenling | Geng P.-W.,Peoples Hospital of Lishui | Wang S.-H.,Peoples Hospital of Lishui | And 4 more authors.
Xenobiotica | Year: 2015

1. CYP2C19 is a highly polymorphic enzyme responsible for the metabolism of a wide range of clinical drugs. Alterations to the CYP2C19 gene contribute to the variability of CYP2C19 enzyme activity, which causes pharmacokinetics and drug efficacies to vary and adverse drug reactions to occur in different persons. Recently, we identified 24 novel CYP2C19 allelic variants in the Chinese Han population. The purpose of present study is to assess the impact of these newly found nucleotide mutations on the enzymatic activity of the CYP2C19 protein.2. Dual-expression vectors were constructed and transiently transfected into 293FT cells. Forty-eight hours after transfection, cells were re-suspended and incubated with two typical probe substrates, omeprazole and S-mephenytoin, to determine the activities of each variant relative to the wild-type protein.3. Immunoblotting results showed that the protein expression levels of the CYP2C19 variants were diverse. Enzymatic ability analysis showed that the variant 35FS exhibited no functional activity, and most of the other variants showed significantly decreased metabolic activities toward both omeprazole and S-mephenytoin compared with wild-type.4. These findings greatly enrich the knowledge of biological effects of these newly found CYP2C19 mutations and aid the application of this knowledge to future individualized drug therapy in clinic. © 2015 Informa UK Ltd.


Chen J.,Zhejiang University | Qian C.,Zhejiang University | Qian C.,First Peoples Hospital of Wenling | Duan H.,Zhejiang University | And 7 more authors.
Journal of Pineal Research | Year: 2015

Neurogenic pulmonary edema (NPE) is a serious non-neurological complication that can occur after a subarachnoid hemorrhage (SAH) and is associated with decreased survival and a poor neurological outcome. Melatonin is a strong antioxidant that has beneficial effects against SAH in rats, including reduced mortality and reduced neurological deficits. The molecular mechanisms underlying these clinical effects in the SAH model, however, have not been clearly identified. This study was undertaken to determine the influence of melatonin on SAH-induced NPE and the potential mechanism of these effects using the filament perforation model of SAH in male Sprague Dawley rats. Either melatonin (150 mg/kg) or a vehicle was given via an intraperitoneal injection 2 hr after an SAH induction. Lung samples were extracted 24 hr after SAH. The results show that the melatonin treatment attenuated SAH-induced NPE by preventing alveolar-capillary barrier dysfunctions via inhibiting the disruption of tight junction proteins (ZO-1 and occludin). Moreover, the treatment downregulated the levels of mature interleukin (IL) -1β, myeloperoxidase (MPO), and matrix metallopeptidase (MMP) 9 expression/activation, which were increased in the lung; also, melatonin treatment improved neurological deficits. Furthermore, the melatonin treatment markedly reduced caspase-3 activity and the number of TUNEL-positive cells in the lung. Taken together, these findings show that administration of melatonin attenuates NPE by preventing alveolar-capillary barrier dysfunctions via repressing the inflammatory response and by anti-apoptosis effects after SAH. © 2015 John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.


Wang W.-J.,First Peoples Hospital of Wenling | Chen J.-Z.,First Peoples Hospital of Wenling | Fang Q.,First Peoples Hospital of Wenling | Li J.-F.,First Peoples Hospital of Wenling | And 2 more authors.
Journal of Laparoendoscopic and Advanced Surgical Techniques | Year: 2013

Objective: To compare the effects of laparoscopic inguinal hernia repair (LIHR) and Lichtenstein tension-free inguinal hernia repair and to explore the safety and feasibility of LIHR as well as the advantages and disadvantages of these procedures. Subjects and Methods: In total, 252 patients with inguinal hernia were equally randomized into the transabdominal preperitoneal (TAPP) repair, totally extraperitoneal (TEP) repair, and Lichtenstein tension-free hernia repair groups (n=84 each). Operating time, postoperative pain scores, postoperative scrotal seroma, postoperative local esthesiodermia, postoperative chronic pains, postoperative long-term hernia relapse, and costs of hospitalization were compared among the three groups. Results: All laparoscopic operations were performed smoothly without intraoperative conversion to open surgery. The LIHR groups showed significantly better effects on postoperative pains and hernia recurrence than the Lichtenstein tension-free herniorrhaphy group (P<.05), but with a significantly higher hospitalization cost (P<.05). The occurrence rate of postoperative scrotal seroma or hydrops in the TAPP, TEP, and Lichtenstein groups was 11 (13.10%), 13 (15.48%), and 6 (7.14%), respectively. No significant differences among the operating time, postoperative local esthesiodermia, or postoperative chronic pains of the groups were observed (P>.05). Conclusions: LIHR is a safe and feasible procedure. It has significantly better effects on postoperative pains and hernia relapse than Lichtenstein tension-free hernia repair. © Mary Ann Liebert, Inc.


PubMed | First Peoples Hospital of Wenling
Type: Journal Article | Journal: Journal of the renin-angiotensin-aldosterone system : JRAAS | Year: 2015

Previous case-control studies on the relation between angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and breast cancer did not reach the same conclusion. In the present study, we aimed to further evaluate the relationship between the ACE gene I/D polymorphism and breast cancer.We selected 13 case-control studies related to ACE gene I/D polymorphism and breast cancer by searching PubMed, EMBase, Chinese Biomedical Literature Database, Chinese CNKI, and Wanfang database. To test the heterogeneity between each study, we utilized the Q-test and I(2) test. To merge the odds ratio (OR) and 95% confidence interval (CI), we utilized the random effects model during the analyses.The present study included 1997 patients with breast cancer and 8404 cancer-free control subjects. By meta-analysis, we did not find any association of ACE gene I/D polymorphism with breast cancer in different model (DD vs (ID+II): OR=1.28, 95% CI (0.90-1.81), p=0.16; II vs (ID+DD): OR=0.99, 95% CI (0.81-1.21), p=0.93; D allele vs I allele: OR=1.15, 95% CI (0.94-1.41), p=0.18).We concluded that ACE gene I/D polymorphism was not associated with breast cancer.

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