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Huang Y.,Southern Medical University | Hu Y.,Southern Medical University | Mai W.,Sun Yat Sen University | Cai X.,First Peoples Hospital of Shunde | And 8 more authors.
Disease Markers | Year: 2011

Objectives: Oxidized low-density lipoprotein (ox-LDL) is considered to be a key factor of initiating and accelerating atherosclerosis. The objective of this study was to investigate the role of ox-LDL in young patients with coronary artery disease (CAD). Methods: 128 consecutive angiographically proven young CAD patients (aged ≤ 55 years) were enrolled, and 132 age-matched non-CAD individuals (coronary angiography normal or negative finding by coronary ultrafast CT) were set as control group. Conventional risk factors (hypertension, dyslipidemia, diabetes mellitus, obesity, smoking) were evaluated in the two groups. Ox-LDL was measured by competitive ELISA. Framingham risk score (FRS) and absolute 10-year CAD events risk were calculated for each individual. Results: Male sex was more prevalent in group CAD than in control (87.5% vs. 62.1%; P< 0.01). There were significant differences in smoking history (P< 0.01) and triglyeride (TG) and ratio of apolipoprotein B/apolipoprotein A1 (ApoB/ApoA1) (both P< 0.05) but no remarkable difference in other conventional risk factors (all P> 0.05) between group CAD and control. Level of ox-LDL was significantly higher in group CAD than in control (P< 0.01). Multivariate logistic regression showed that male sex (OR, 4.54; 95%CI, 1.76-9.77), smoking quantity (OR, 2.78; 95%CI, 1.34-4.25), TG (OR, 1.42; 95%CI, 1.18-2.83), ApoB/ApoA1 (OR, 1.73; 95%CI, 1.32-4.23), and ox-LDL (OR, 2.15; 95%CI, 1.37-6.95) were independently correlated with CAD in young patients. Area under the curve (AUC) of receiver operating characteristic (ROC) curve of TG, ApoB/ApoA1, and ox-LDL was 0.831, 0.866, and 0.935, respectively (P< 0.001). Conclusions: Ox-LDL is an important independent risk factor for CAD in young patients after adjusting other risk factors such as smoking, TG, and ApoB/ApoA1. © 2011 - IOS Press and the authors. All rights reserved. Source

Jiang Y.,First Peoples Hospital of Shunde | Xiao Q.,Jiangxi Province Peoples Hospital | Hu Z.,Chongqing Medical University | Pu B.,Kunming Medical College | And 4 more authors.
Orthopedics | Year: 2014

The objective of this study was to observe the expression of leukemia inhibitory factor (LIF) in animals and in different clinical grades of patient osteoarthritic tissues. Thirty-five rabbits were used in a Colombo model of experimental osteoarthritis (OA). Five rabbits each were sacrificed on postoperative days 3, 7, 14, 28, 42, 56, and 84. Immunohistochemistry analysis for LIF expression and distribution in the cartilage and synovium of animals was performed at these times. Sixty-seven samples of human articular tissue were obtained from patients with different grades of OA according to symptoms and radiographic inspection. The mRNA expression of LIF was determined by reverse transcription polymerase chain reaction, and LIF protein was determined by enzyme-linked immunosorbent assay (ELISA). The results showed a slight expression of LIF in normal cartilage tissue but less in synovium tissue; however, the expression of LIF was marked in synovial lining cells and superficial and middle-layer cartilage in animal OA (P<.05). Leukemia inhibitory factor mRNA was expressed at the highest level in moderate degrading subchondral bone, and LIF was expressed at the highest level in seriously degrading articular cartilage tissue. These results were similar to those found with ELISA. This study suggests that LIF in OA articular tissues varies by clinical symptoms and grade. It plays an important role in the pathogenesis of OA. Source

Chen J.,Southern Medical University | Wang J.,Southern Medical University | Huang Y.,Southern Medical University | Lai X.,Guangzhou University | And 5 more authors.
PLoS ONE | Year: 2014

Objective: To study the influence of acupuncture at Waiguan (TE5) on the functional connectivity of the central nervous system of patients with ischemic stroke. Methods: Twenty-four patients with ischemic stroke in the left basal ganglia were randomized based on gender to receive TE5 acupuncture (n = 12) or nonacupoint acupuncture (n = 12). Each group underwent sham acupuncture and then verum acupuncture while being scanned with functional magnetic resonance imaging. Six regions of interest (ROI) were defined, including bilateral motor, somatosensory, and bilateral basal ganglia areas. The functional connectivity between these ROIs and all voxels of the brain was analyzed in Analysis of Functional NeuroImages(AFNI) to explore the differences between verum acupuncture and sham acupuncture at TE5 and between TE5 acupuncture and nonacupoint acupuncture. The participants were blinded to the allocation. Result: The effect of acupuncture on six seed-associated networks was explored. The result demonstrated that acupuncture at Waiguan (TE5) can regulate the sensorimotor network of the ipsilesional hemisphere, stimulate the contralesional sensorimotor network, increase cooperation of bilateral sensorimotor networks, and change the synchronization between the cerebellum and cerebrum. Furthermore, a lot of differences of effect existed between verum acupuncture and sham acupuncture at TE5, but there was little difference between TE5 acupuncture and nonacupoint acupuncture. Conclusion: The modulation of synchronizations between different regions within different brain networks might be the mechanism of acupuncture at Waiguan (TE5). Stimulation of the contralesional sensorimotor network and increase of cooperation of bilateral hemispheres imply a compensatory effect of the intact hemisphere, whereas changes in synchronization might influence the sensorimotor function of the affected side of the body. Trial Registration: Chinese Clinical Trial Registry ChiCTR-ONRC-08000255. © 2014 Chen et al. Source

Han Y.,Sun Yat Sen University | Zeng F.,Sun Yat Sen University | Tan G.,Sun Yat Sen University | Yang C.,Guangzhou University | And 5 more authors.
Cellular Physiology and Biochemistry | Year: 2013

Background/Aim: The abnormal activation of the AKT/GSK3β signal pathway in lymphocytes from systemic lupus erythematosus (SLE) patients plays an important role in the pathogenesis of the disease. Recently Hydrogen sulfide (H2S) has been recognized as a crucial gaseous signaling molecule, involved in regulation of cell proliferation. However, the role of H 2S in regulating the abnormal activation of lymphocytes from SLE patients has not been established. This study was conducted to investigate the effect of H2S on lymphocytes and to explore the mechanisms involved. Methods: The lymphocytes were isolated from SLE patients with or without renal disease and healthy controls. The cells were treated as indicated in each experiment. Cell viability was analyzed by CCK-8. Cell cycle distribution was determined by flow cytometry. Western blot was used to detect the expression of phosphorylated AKT (ser473), GSK3β (ser9) and CDK2, p27Kip1 and p21WAF1/CIP1. Results: Our findings showed that proliferation of lymphocytes was stimulated following treatment with NaHS (a H2S donor) at low NaHS concentrations (<1mM) but inhibited at high NaHS concentrations (>2mM). Similar results were observed using GYY4137, which is a slow-releasing H2S donor. Pretreatment of lymphocytes from SLE patients with NaHS at high concentrations prior to exposure to phytohemagglutinin (PHA) significantly attenuated proliferation, evidenced by decrease in cell viability and S phase distribution of cell cycle. Pretreatment with NaHS decreased PHA-induced expression of CDK2, phosphorylation levels of AKT (ser473) and GSK3β (ser9) and increased the expression of p27 Kip1 and p21WAF1/CIP1. Moreover, pretreatment with NaHS blunted the stimulation of SLE lymphocyte proliferation by GSK3β inhibitor lithium chloride. Conclusion: These results demonstrate that H2S inhibits the abnormal activation of lymphocytes from SLE patients throuqh the AKT/GSK3β signal pathway. Copyright © 2013 S. Karger AG, Basel. Source

Yang Y.,First Peoples Hospital of Shunde | Bi M.,Southern Medical University | Xiao L.,First Peoples Hospital of Shunde | Chen Q.,First Peoples Hospital of Shunde | And 5 more authors.
European Journal of Internal Medicine | Year: 2015

Background and purpose Young Chinese male adults have faced increasing psychological stress. Whether this is associated with the increased risk of coronary artery disease (CAD) in young Chinese males remains unclear. This study aimed to evaluate the correlation and underlying mechanisms of perceived stress and CAD in young male patients. Methods A total of 178 male patients diagnosed as young CAD (aged ≤ 55 years) by coronary angiography (CAG) were enrolled, and 181 age-matched non-CAD individuals were set as control group. Traditional cardiovascular risk factors and levels of epinephrine and norepinephrine were measured, and perceived stress status was accessed by Perceived Stress Scale (PSS). Results The PSS score was correlated with levels of epinephrine (r = 0.45), norepinephrine (r = 0.41), high-sensitivity C-reactive protein (hs-CRP) (r = 0.38, p < 0.01), and current smoking (r = 0.32) (all p <0.05). Multivariate logistic regression analysis showed that smoking (OR, 3.12; 95%CI, 1.23-7.91), triglycerides (OR, 1.42; 95%CI, 1.04-1.94), hs-CRP (OR, 3.57; 95%CI, 1.65-7.72), and PSS score (OR, 1.81; 95%CI, 1.23-2.66) were independently correlated with CAD in young patients. The association between PSS score and risk of CAD become insignificant (OR, 1.43; 95%CI, 0.96-2.13) when further adjusted for the levels of epinephrine and norepinephrine. Conclusions After adjustment for multiple cardiovascular risk factors, high perceived stress was an independent risk factor for CAD in young Chinese male patients. Abnormal activation of the sympathetic nervous system may play an important role linking perceived stress with the risk of CAD. ;copy; 2015 European Federation of Internal Medicine. Source

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