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Yan J.-Q.,Affiliated Hospital of Shaoyang Medical College | Tan C.-Z.,First Peoples Hospital of Shaoyang City | Tan C.-Z.,University of South China | Wu J.-H.,Nanhua University | And 8 more authors.
Molecular and Cellular Biochemistry | Year: 2013

To investigate the effects of neopterin on ABCA1 expression and cholesterol efflux in human THP-1 macrophage-derived foam cells, and to explore the role of the liver X receptor alpha (LXRα) involved. In the present study, THP-1 cells were pre-incubated with ox-LDL to become foam cells. The protein and mRNA expression were examined by Western blot assays and real-time quantitative PCR, respectively. Liquid scintillation counting and high performance liquid chromatography assays were used to test cellular cholesterol efflux and cholesterol content. Neopterin decreased ABCA1 expression and cholesterol efflux in a time- and concentration-dependent manner in THP-1 macrophage-derived foam cells, and the LXRα siRNA can reverse the inhibitory effects induced by neopterin. Neoterin has a negative regulation on ABCA1 expression via the LXRα signaling pathway, which suggests the aggravated effects of neopterin on atherosclerosis. © 2013 Springer Science+Business Media New York. Source

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