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Li L.,First Peoples Hospital of Kunming City | Zhong H.,Kunming Medical University | Tian E.,Kunming Medical University | Yu M.,Sun Yat Sen University | And 8 more authors.
Cornea | Year: 2015

Purpose: To determine the 5-year cumulative incidence of pterygium and its associated predictors in the Bai Chinese population in a rural community. Methods: This population-based study included 2133 subjects aged 50 years or older in 2010 and was repeated in 2015 with 1520 subjects (71.3%) participating in the follow-up examination. Participants with pterygium in either eye in 2010 were excluded from the analysis related to incidence. Anterior segment examination was performed without pupil dilation using a slit lamp, and pterygium was defined as a raised fleshy triangular fibrovascular tissue growth of the conjunctiva encroaching onto the clear cornea. Results: The 5-year cumulative incidence of pterygium was 6.8% [95% confidence interval (CI), 5.2-8.4] and was significantly higher in women compared with men (8.8% vs. 3.8%; P = 0.003). The agespecific incidence was 7.7%, 6.5%, and 5.6% in those aged 50-59, 60-69, 70 years, or older at the baseline, respectively. Outdoor occupation was the only predictor, which remained to be signifi-cantly associated with a higher incidence of pterygium in multivariate analysis (odds ratio = 2.52, 95% CI, 1.27-4.95). The predictive effect of outdoor occupation on incident pterygium was moderate with an area under the curve in the receiver operating characteristic analysis of 0.59 (95% CI, 0.53-0.66). Conclusions: The incidence of pterygium in this rural cohort was higher compared to a previous report in Chinese in urban areas. The findings are important for health policy makers to project future burden of pterygium and make proper decisions on health resource allocation. © 2015 Wolters Kluwer Health, Inc. All rights reserved. Source

Xu Y.,Center for Hepatology | Chen X.-P.,First Peoples Hospital of Kunming City | Yang J.-H.,Center for Hepatology | Zhang Y.-Z.,First Peoples Hospital of Kunming City | And 2 more authors.
World Chinese Journal of Digestology | Year: 2010

AIM: To investigate whether treatment with ammonia induces apoptosis and necrosis of rat astrocytes in vitro and to analyze the pathogenesis of hepatic encephalopathy. METHODS: Primary astrocytes isolated from the cerebral cortex of newborn rats were cultured in vitro and identified by immunocytochemistry (positive for glial fibrillary acidic protein). Cells were then randomly assigned to low-concentration ammonia group (treated with 2.5 mmol/L NH 4Cl), high-concentration ammonia group (treated with 5 mmol/L NH 4Cl), and control group (untreated). After culture for 24, 48, and 72 h, cell apoptosis and necrosis were detected by flow cytometry. RESULTS: Treatment with ammonia at a concentration of 2.5 mmol/L could induce astrocyte apoptosis and necrosis, and apoptosis-and necrosis-inducing activity of ammonia was concentration- and time-dependent. Compared with the control group, the apoptosis and necrosis rates of astrocytes in the high-concentration ammonia group were significantly higher (24 h: 12.5% ± 4.0% vs 7.7% ± 1.9%, 9.3% ± 1.6% vs 6.3% ± 0.7%; 48 h: 17.7% ± 4.2% vs 8.5% ± 1.3%, 10.5% ± 2.8% vs 7.2% ± 1.1%; 72 h: 23.9% ± 4.1% vs 9.6% ± 1.9%, 11.4% ± 2.5% vs 7.9% ± 1.5%, all P < 0.05). CONCLUSION: Treatment with ammonia could induce astrocyte apoptosis and necrosis in vitro in a time- and concentration-dependent manner. Source

Li J.-Y.,First Peoples Hospital of Kunming City | Wang D.-Y.,Kunming Medical University | Yang J.-W.,First Peoples Hospital of Kunming City | Zhao N.,First Peoples Hospital of Kunming City | And 5 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Nerve growth factor plays an irreplaceable role on the survival, differentiation and maintenance of the central nervous system cells, also promote the growth and development of these cells. Objective: To explore effect of Breviscapine on the growth of in vitro cultured neurons in cerebral cortex of Sprague-Dawley rats, and preliminarily investigate the action mechanism. Methods: The cerebral cortex of newborn Sprague-Dawley rat embryos was collected, the neurons were primary cultured and randomly divided into three groups: normal control group (no treatment), control group (neurons were cultured with normal saline), Breviscapine group (neurons were cultured with 10 g/L Breviscapine). Furthermore each group was assigned into 24-hour and 48-hour subgroups. Images were captured from the 24-well plates at each time points. RT-PCR was applied to examine the expression of nerve growth factor and TrkA mRNA. MTT was used to detect the neuronal growth at each time point. Results And Conclusion: There was no difference between normal control group and control group in the cell counts, cell body area, neurite length and viability (P > 0.05), as the time prolonged, all data were raised in the two groups (P < 0.05). Breviscapine group showed a higher cell count, cell body area, neurite length and viability than normal control group and control group (P < 0.05). No significant difference was found in the expression of nerve growth factor and TrkA mRNA between normal control group and control group (P > 0.05). At each time point, these data in Breviscapine group were increased compared with normal control group and control group (P < 0.05). Breviscapine can promote the survival and growth of brain-derived neurons in Sprague-Dawley rats, and the mechanism might depend on the up-regulating expression of nerve growth factor and its receptor TrkA. Source

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