Sun X.,First Peoples Hospital of Kunming |
Liao N.-K.,Shanghai JiaoTong University |
Yu J.-J.,Shanghai JiaoTong University
Journal of International Medical Research | Year: 2012
Objective: To evaluate the utility of a mitochondrial functional score in predicting the progress of prostate cancer. Methods: This retrospective study included 72 patients (mean age 70.1 years) with prostate cancer who were treated by radical prostatectomy between October 2006 and March 2007. The epithelio - glandular mitochondrial functional scores were assessed according to the Flameng grading. Patients were divided into six groups (groups 1 - 6) according to their Gleason score (Gleason score 2 - 7, respectively). The correlation between Gleason score and mitochondrial functional score was examined using Pearson's correlation coefficient. Results: The mean mitochondrial functional score was significantly lower in group 6 compared with group 1. An inverse correlation was found between the Gleason and mitochondrial functional scores. At 1 year, significantly fewer patients in group 1 had died (0/15 patients) than in group 6 (2/10 patients); the deaths were cancerrelated. Conclusion: Mitochondrial dysfunction exists in patients with prostate cancer, particularly in cases with a higher degree of malignancy. The mitochondrial functional score, combined with the Gleason score, is beneficial for predicting the progress of prostate cancer. © 2012 Field House Publishing LLP.
Guan Z.,Prince of Songkla University |
Guan Z.,First Peoples Hospital of Kunming |
Shi S.,University of Southern California |
Samruajbenjakun B.,Prince of Songkla University |
Kamolmatyakul S.,Prince of Songkla University
Bio-Medical Materials and Engineering | Year: 2015
Chitosan has been used as scaffolds with various methods of fabrication including expensive commercial available ones for tissue engineering. The objective of this study is to assemble our novel method of chitosan scaffold fabrication in economical and uncomplicated way that suitable for dental pulp stem cell (DPSC) and stem cells of human exfoliated deciduous teeth (SHED). Chitosan scaffolds (2% and 3%) were fabricated in an uncomplicated procedure, including centrifugation and freeze-drying steps. The chitosan scaffolds were compared and the pore size, swelling and degradation were assessed. In addition, the cytocompatibility was assessed of chitosan scaffolds seeded with DPSC and SHED. The pore size of 2% and 3% chitosan scaffolds were similar being 188.71±51.90 μm and 195.30±67.21 μm, respectively. Swelling ratios of 3% chitosan scaffolds were significantly lower than those of 2% chitosan scaffolds. Dimension of scaffolds changed in first 5 minutes. After that, those scaffolds could maintain their dimension. Chitosan scaffolds degraded as from day 7. No differences were found between 2% and 3% chitosan scaffolds. The scaffolds were shown to be non-toxic and to promote DPSCs and SHED growth. The viability of DPSCs and SHED on 2% scaffolds proved to be higher than that of the 3% scaffold group. This study suggested that chitosan scaffolds fabricated with our novel method were suitable for the growth and survival of DPSC and SHED. © 2015 - IOS Press and the authors.
Che Y.,Prince of Songkla University |
Che Y.,First Peoples Hospital of Kunming |
Assanangkornchai S.,Prince of Songkla University |
Mcneil E.,Prince of Songkla University |
And 5 more authors.
Drug and Alcohol Review | Year: 2010
Introduction and Aim. The aim of this study is to identify the predictors of early dropout of patients in methadone maintenance treatment (MMT) clinics inYunnan province, China. Methods. A cohort study was conducted on 218 patients starting treatment in five MMT clinics between 1 March 2008 and 31 August, with follow up to 28 February 2009. Patients were interviewed using a semistructured questionnaire covering socio-demographic characteristics and drug abuse history. Attendance at clinic and daily dose were abstracted from the clinic records. The mean average daily dose per patient in each period was compared across three periods, 0-1, >1-3 and >3-6 months, using analysis of variance and random-intercept mixed linear regression modelling. Cox regression model with time-varying average daily dose within each period was performed to identify factors predicting dropout in the MMT program. Results. Cumulative probability of retention at 1, 3 and 6 months was 94%, 75% and 57%, respectively. There was no relationship between dose and probability of dropout in periods 1 and 2. However, after 3 months higher average daily dose (>60 mg) was associated with lower probability of dropout. Dropout was more likely among the Han ethnic group [hazard ratio (HR) = 1.86, 95% confidence interval (CI): 1.65-3.26], in those who had to spend over 30 min to visit the clinic (HR = 1.63, 95% CI: 1.07-2.49) and in those living with other drug users (HR = 2.71,95% CI: 1.55-4.74). Discussion and Conclusion. Patients' early dropout was related to ethnicity, clinic accessibility, living with drug users and methadone dose. A higher methadone dose as appropriate for maintenance treatment is recommended. © 2010 Australasian Professional Society on Alcohol and other Drugs.
Yang P.,First Peoples Hospital of Kunming |
Guo T.,Xichang College |
Wang W.,First Peoples Hospital of Kunming |
Peng Y.-Z.,Xichang College |
And 6 more authors.
Heart and Vessels | Year: 2013
Our aim was to evaluate the safety and effectiveness of extracorporeal cardiac shock wave therapy (CSWT) for the patients with coronary heart disease (CHD) using a randomized, double-blind, controlled clinical trial design. Twenty-five patients with CHD were enrolled in this study. Fourteen of the patients were randomized into the CSWT group and 11 into the control group. We applied the CSWT procedure to each patient by using nine shock treatments during 3 months, but the shock wave (SW) energy was only applied to the patients in the CSWT group and not to the patients in the control group. Technetium-99m sestamibi myocardial perfusion, fluorine-18 fluorodeoxyglucose myocardial metabolism single-photon emission computed tomography (SPECT), and two-dimensional echocardiography were performed to identify segments of myocardial ischemia, myocardial viability, and ejection fraction before and after CSWT. We also followed the patients to evaluate adverse effects. After CSWT, the New York Heart Association class, the Canadian Cardiovascular Society angina scale, nitroglycerin dosage, myocardial perfusion and myocardial metabolic imaging scores of dual-isotope SPECT in the CSWT group were reduced significantly (P = 0.019, 0.027, 0.039, 0.000, 0.001, respectively), and the Seattle Angina Questionnaire scale, 6-min walking test, and left ventricular ejection fraction were increased significantly (P = 0.021, 0.024, 0.016, respectively) compared with those before the SW treatment. All of the parameters in the control group did not change significantly after the treatment (all P > 0.05). No serious adverse effects of CSWT were observed. Cardiac shock wave therapy is a safe and effective treatment for CHD patients. © 2012 Springer.
Qin W.,First Peoples Hospital of Kunming
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2011
The objective is to obtain the information of women in the period of prepregnancy and pregnancy infected by TORCH (Toxoplasma, Rabella Virus, Cytomegalovirus, and Herpes Simplex Virus) in Kunming for preventing fetus from infection by TORCH in uterus. The serum IgM and IgG specific for TORCH from 1307 women in the period of pre-pregnancy and pregnancy were measured with ELISA and The reagent is the product of virion/serion. The serum IgM specific for TORCH were detected in 2.83% of 1307 pregnant women for Toxoplasma, 2.37% for Rebulla Virus, 0.46% for Cytomegalovirus, 2.45% for Herpes Simplex Virus. The total positive rate of serum IgM specific for TORCH was 1.45%. The serum IgG specific for TORCH were detected in 3.98% of 1307 pregnant women for Toxoplasma, 72.3% for Rebulla Virus, 97.78% for Cytomegalovirus, 80.34% for Herpes Simplex Virus. The total positive rate of serum IgG specific for TORCH was 63.60%. There was no significant difference among the women with different pregnant situation in terms of the serum IgM and IgG specific for TORCH. There are some of women in the period of pre-pregnancy and pregnancy with current infection by TORCH in Kunming. It is recommended to screen for infection by TORCH in women in the period of pre-pregnancy and pregnancy to prevent fetus from infection by TORCH in uterus.
Huang M.-P.,First Peoples Hospital of Kunming |
Xu H.,First Peoples Hospital of Kunming |
Pan M.,First Peoples Hospital of Kunming
Journal of Clinical Dermatology | Year: 2016
A case of pelvic Castleman disease complicated by paraneoplastic pemphigus is reported. A 13-year-old boy presented with oral mucosal erosion for over 3 months. Physical examination showed extensive erosions on his canthi, lips, oral mucosa and tongue, with hemorrhagic crusts on the surface. Other abnormalities included fingernail deformation and onycholysis, nailfold erythema, erosion on the glans penis, sulcus coronaries and anus. A lump could be touched on the lower left abdomen 23 cm to McBurney point. The whole body PET/CT showed a huge irregular mass in the pelvic cavity with calcification. Histopathological examination of the resected tissue revealed angiofollicular lymph node hyperplasia in the pelvic cavity, with hyaline vascular type. The final diagnose was the pelvic Castleman disease complicated by paraneoplastic pemphigus. © 2016, Editorial Board of Journal of Clinical. All rights reserved.
Liu W.-H.,Yunnan University |
Zhao Y.-S.,Yunnan University |
Gao S.-Y.,Chuxiong Normal University |
Li S.-D.,Kunming Medical College |
And 3 more authors.
American Journal of Pathology | Year: 2010
Elevated homocysteine levels are defined as hyperhomocysteinemia (HHcy), a disorder that is associated with cardiovascular and neurodegenerative diseases as well as with hepatic fibrosis. Recent studies have shown that HHcy promotes hepatic injury by increasing oxidative stress. Although homocysteine induces cell cycle arrest in a variety of different cell types, it is not known whether HHcy has a definitive role in hepatocyte proliferation during liver regeneration. In this report, we investigated the effect of homocysteine on liver regeneration. Our results demonstrated that mice with HHcy exhibited an impairment in liver regeneration after partial hepatectomy, as measured by immunohistochemical staining of proliferation cell nuclear antigen and bromodeoxyuridine incorporation. Impaired proliferation was also correlated with reduced cyclin D1 induction and elevated expression levels of both p53 and p21Cip1. In addition, the phosphorylation of Akt, which plays an essential role in normal regeneration responses, was attenuated during the early phases of liver regeneration in HHcy mice. Our results also indicated that the cAMP/protein kinase A pathway mediated the inhibitory effect of homocysteine on liver regeneration. These findings provide evidence that impairment of liver regeneration by HHcy may result in delayed recovery from liver injury induced by homocysteine itself. Copyright © American Society for Investigative Pathology.
Liu Q.-Y.,First Peoples Hospital of Kunming |
Zhang W.-Z.,Chinese People's Liberation Army |
Xia H.-T.,Chinese People's Liberation Army |
Leng J.-J.,Chinese People's Liberation Army |
And 4 more authors.
World Journal of Gastroenterology | Year: 2014
METHODS: The data from 196 consecutive patients who underwent pancreaticoduodenectomy, performed by different surgeons, in the General Hospital of the People's Liberation Army between January 1st, 2013 and December 31st, 2013 were retrospectively collected for analysis. The diagnoses of POPF and clinically relevant (CR)-POPF following pancreaticoduodenectomy were judged strictly by the International Study Group on Pancreatic Fistula Definition. Univariate analysis was performed to analyze the following factors: patient age, sex, body mass index (BMI), hypertension, diabetes mellitus, serum CA19-9 level, history of jaundice, serum albumin level, blood loss volume, pancreatic duct diameter, pylorus preserving pancreaticoduodenectomy, pancreatic drainage and pancreaticojejunostomy. Multivariate logistic regression analysis was used to determine the main independent risk factors for POPF.RESULTS: POPF occurred in 126 (64.3%) of the patients, and the incidence of CR-POPF was 32.7% (64/196). Patient characteristics of age, sex, BMI, hypertension, diabetes mellitus, serum CA19-9 level, history of jaundice, serum albumin level, blood loss volume, pylorus preserving pancreaticoduodenectomy and pancreaticojejunostomy showed no statistical difference related to the morbidity of POPF or CR-POPF. Pancreatic duct diameter was found to be significantly correlated with POPF rates by univariate analysis and multivariate regression analysis, with a pancreatic duct diameter ≤ 3 mm being an independent risk factor for POPF (OR = 0.291; P = 0.000) and CR-POPF (OR = 0.399; P = 0.004). The CR-POPF rate was higher in patients without external pancreatic stenting, which was found to be an independent risk factor for CR-POPF (OR = 0.394; P = 0.012). Among the entire patient series, there were three postoperative deaths, giving a total mortality rate of 1.5% (3/196), and the mortality associated with pancreatic fistula was 2.4% (3/126).CONCLUSION: A pancreatic duct diameter ≤ 3 mm is an independent risk factor for POPF. External stent drainage of pancreatic secretion may reduce CR-POPF mortality and POPF severity.AIM: To explore the morbidity and risk factors of postoperative pancreatic fistula (POPF) following pancreaticoduodenectomy. © 2014 Baishideng Publishing Group Inc. All rights reserved.
Wang Q.,First Peoples Hospital of Kunming |
Wu Q.,Tianjin Haihe Hospital |
Feng J.,Tianjin Medical University |
Feng J.,Duke University |
Sun X.,Tianjin Haihe Hospital
Patient Preference and Adherence | Year: 2013
Obstructive sleep apnea (OSA) occurs in 4% of middle-aged men and 2% of middle-aged women in the general population, and the prevalence is even higher in specific patient groups. OSA is an independent risk factor for a variety of cardiovascular diseases. Endothelial injury could be the pivotal determinant in the development of cardiovascular pathology in OSA. Endothelial damage ultimately represents a dynamic balance between the magnitude of injury and the capacity for repair. Bone marrow-derived endothelial progenitor cells (EPCs) within adult peripheral blood present a possible means of vascular maintenance that could home to sites of injury and restore endothelial integrity and normal function. Methods: We summarized pathogenetic mechanisms of OSA and searched for available studies on numbers and functions of EPCs in patients with OSA to explore the potential links between the numbers and functions of EPCs and OSA. In particular, we tried to elucidate the molecular mechanisms of the effects of OSA on EPCs. Conclusion: Intermittent hypoxia cycles and sleep fragmentation are major pathophysiologic characters of OSA. Intermittent hypoxia acts as a trigger of oxidative stress, systemic inflammation, and sympathetic activation. Sleep fragmentation is associated with a burst of sympathetic activation and systemic inflammation. In most studies, a reduction in circulating EPCs has emerged. The possible mechanisms underlying the decrease in the number or function of EPCs include prolonged inflammation response, oxidative stress, increased sympathetic activation, physiological adaptive responses of tissue to hypoxia, reduced EPC mobilization, EPC apoptosis, and functional impairment in untreated OSA. Continuous positive airway pressure (CPAP) therapy for OSA affects the mobilization, apoptosis, and function of EPCs through preventing intermittent hypoxia episodes, improving sleep quality, and reducing systemic inflammation, oxidative stress levels, and sympathetic overactivation. To improve CPAP adherence, the medical staff should pay attention to making the titration trial a comfortable first CPAP experience for the patients; for example, using the most appropriate ventilators or proper humidification. It is also important to give the patients education and support about CPAP use in the follow-up, especially in the early stage of the treatment. © 2013 Wang et al.
Li L.,First Peoples Hospital of Kunming |
Zhang S.-N.,First Peoples Hospital of Kunming |
Ran J.-H.,First Peoples Hospital of Kunming |
Liu J.,First Peoples Hospital of Kunming |
And 2 more authors.
Chinese Medical Sciences Journal | Year: 2011
Objective: To investigate the mechanism of immune hyporesponsiveness induced by donor-antigenunloaded recipient-derived immature dendritic cell (imDC) of liver grafts in rats. Methods: Forty Sprague-Dawley rats (donor) and forty male Wistar rats (recipient) were randomly divided into 4 groups: control, cyclosporine A (CsA), mature DC (mDC), and imDC groups respectively, with 10 donor rats and 10 recipient rats in each group. Recipient rats in CsA group were treated with 10 mg kg-1 d-1 CsA starting day 2 after the transplantation. Recipients in the mDC or imDC groups were given Wistar rat derived mDCs (1×106/rat) or imDCs (1×106/rat) via dorsal vein of the penis respectively 1 day before the transplantation. In each group, 5 recipients were kept for determination of survival time and the other 5 rats were executed at day 10 after transplantation. Blood samples were collected for the measurement of serum alanine aminotransferase (ALT), total bilirubin (TBIL), interleukin 2 (IL-2), interferon gamma (IFN-γ), IL-4, and IL-10 levels. Liver tissue was harvested for HE staining and acute rejection evaluation. Expression levels of Fas-L/Fas in the grafts were detected by immunohistochemical staining; and Western blot was used to detect the expression level of Scurfin. Results The survival time of CsA and imDC groups was significantly longer than that of control and mDC groups (all P<0.05). The levels of serum ALT and TBIL in the control group (2072.20±217.93 IU/L and 147.42±22.02 μmol/L) and mDC group (2117.00±285.13 IU/L and 141.58±20.82 μmol/L) were significantly higher than those in the CsA group (59.68±13.48 IU/L and 15.40±2.13 μmol/L) or imDC group (50.80±9.63 IU/L and 14.44±3.49 μmol/L) (all P<0.05). In the CsA and imDC groups, the levels of IL-2 (22.52±3.75 pg/mL and 22.12±3.90 pg/mL) and IFN-γ (309.20±25.19 pg/mL and 321.00±21.64 pg/mL) were significantly lower, but the levels of IL-4 (297.60±25.07 pg/mL and 277.00±22.47 pg/mL) and IL-10 (1226.00±140.49 pg/mL and 1423.00±106.39 pg/mL) were higher than those of the control (IL-2: 147.78±12.80 pg/mL, IFN-γ: 1758.60±106.22 pg/mL, IL-4: 17.40±4.77 pg/mL, IL-10: 81.00± 9.47 pg/mL) and mDC groups (IL-2: 142.34±9.29 pg/mL, IFN-γ: 1835.00±82.63 pg/mL, IL-4: 15.60± 3.96 pg/mL, IL-10: 68.80±11.23 pg/mL) (all P<0.01). The expression level of Scurfin protein on CD4+CD25+ T cells of the imDC group (1.34±0.29) was significantly higher than that in the control (0.72±0.13), CsA (0.37±0.11), and mDC groups (0.78±0.17) (all P<0.05). Conclusion: Donor-antigen-unloaded recipient-derived imDC is an effective treatment in inducing immune hyporesponsiveness through induction of T cell apoptosis, shift in Thl/Th2 balance, and proliferation of regulatory T cell.