Hou X.,First Peoples Hospital of Jingzhou |
Zhang P.,First Peoples Hospital of Jingzhou |
Nie W.,Shanghai Changzheng Hospital |
Tang S.,First Peoples Hospital of Jingzhou |
And 5 more authors.
JRAAS - Journal of the Renin-Angiotensin-Aldosterone System | Year: 2015
Background: Previous studies investigating the association between the angiotensin-converting enzyme I/D polymorphism and sepsis have provided inconsistent results. Thus, a meta-analysis was performed to clarify the effect of the angiotensin- converting enzyme I/D polymorphism on sepsis risk and sepsis-related mortality. Methods: A comprehensive literature search was performed. Six casecontrol studies and two cohort studies were included in this meta-analysis. Odds ratios and corresponding 95% confidence intervals were estimated using randomeffects models. Results: Pooled analysis of six casecontrol studies showed that there was a significant association between the angiotensin- converting enzyme I/D polymorphism and sepsis risk in a recessive genetic model (odds ratio = 0.75, 95% confidence interval 0.620.91, p = 0.004). However, no significant association between the angiotensin-converting enzyme I/D polymorphism and mortality was observed (odds ratio = 0.84, 95% confidence interval 0.571.24, p = 0.38). Conclusions: Our meta-analysis confirmed that the angiotensin-converting enzyme I/D polymorphism was associated with sepsis risk. However, the angiotensin-converting enzyme I/D polymorphism was not associated with sepsis mortality. © 2015 The Author(s).
Chen H.,Central Hospital of Shanghai Zhabei District |
Fang Y.,Central Hospital of Shanghai Zhabei District |
Zhu H.,Tongji University |
Li S.,Tongji University |
And 11 more authors.
Molecular Medicine Reports | Year: 2014
The aim of this study was to identify the molecular events that distinguish serrated colorectal carcinoma (SCRC) from conventional colorectal carcinoma (CCRC) through differential gene expression, pathway and protein-protein interaction (PPI) network analysis. The GSE4045 and GSE8671 microarray datasets were downloaded from the Gene Expression Omnibus database. We identified the genes that are differentially expressed between SCRC and normal colon tissues, CCRC and healthy tissues, and between SCRC and CCRC using Student's t-tests and Benjamini-Hochberg (BH) multiple testing corrections. The differentially expressed genes (DEGs) were then mapped to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and their enrichment for specific pathways was investigated using the Database for Annotation, Visualization and Integrated Discovery (DAVID) tool with a significance threshold of 0.1. Analysis of the potential interactions between the protein products of 220 DEGs (between CCRC and SCRC) was performed by constructing a PPI network using data from the high performance RDF database (P<0.1). The interaction between pathways was also analyzed in CCRC based on the PPI network. Our study identified thousands of genes differentially expressed in SCRC and CCRC compared to healthy tissues. The DEGs in SCRC and CCRC were enriched in cell cycle, DNA replication, and base excision repair pathways. The proteasome pathway was significantly enriched in SCRC but not in CCRC after BH adjustment. The PPI network showed that tumour necrosis factor receptor-associated factor 6 (TRAF6) and atrophin 1 (ATN1) were the most central genes in the network, with respective degrees of node predicted at 90 and 88. In conclusion, the preoteasome pathway was shown to be specifically enriched in SCRC. Furthermore, TRAF6 and ATN1 may be promising biomarkers for the distinction between serrated and conventional CRC.
Xiong X.-B.,First Peoples Hospital of Jingzhou |
Cheng X.-L.,First Peoples Hospital of Jingzhou |
Xiang M.-Q.,First Peoples Hospital of Jingzhou
Journal of Clinical Neurology | Year: 2010
Objective: To explore the influence of Edaravone on tumor necrosis factor(TNF)-α and interleukin (IL)-1β expression at hippocampus in epileptic rats. Methods: Thirty-six Wistar rats were randomly divided into Edaravone group, epilepsy group and normal control group. Epilepsy models of rats were prepared with intraperitoneal injection of pentylenetetrazol. Edaravone group were intraperitoneally injected Edaravone(3 mg/kg) at 0.5 h before, right after and 12 h after the operation. The behaviour of epileptic rats was observed. And the expressions of TNF-α and IL-1β at hippocampus in all the rats were detected by enzyme linked immunosorbent assay (ELISA). Results: After operation, all the 12 rats of epilepsy group were at stage V seizure; while in Edaravone group, 3 rats were at stage V seizure, 2 rats were at stage IV seizure, 5 rats were at stage III seizure,2 rats were at stage II seizure. Compared with normal control group, the expressions of TNF-α and IL-1β in Edaravone group and epilepsy group were significantly increased (P < 0.05-0.01). Compared with epilepsy group, the expressions of TNF-α and IL-1β in Edaravone group were significantly decreased (all P <0.01). Conclusion: Edaravone can remarkably decrease the expression of TNF-α and IL-1β at hippocampus in epileptic rats, which results in anti-epilepsy effect.