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Zhao Y.,First Peoples Hospital of Jingmen
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

To explore the clinical use of sieving detection among the childhood with allergic disease. The sieving detection about allergen inhalant allergens, Fx5 in the CAP anaphylactogen detection system, and serum specific IgE were detected in three hundred and thirty-one cases of children (aged from 1 year to 14 years old) with allergic disease. Patients were divided into group 1, group 2 and group 3 according to the age from 0 to 3, 3 to 6, and 6 to 14 years old. All datas were statistical analysed among different age groups. Among the 331 patients, the positive rate of allergic sieving detection was 67.98%, the elevation rate of IgE was 53.78%. Inhalant allergen positive rate was 60.42%, while the food allergen positive rate was 28.10%. Inhalant allergen positive rate of the group 3 (aged from 6 to 14 years old) was significant higher than the other two age groups (68.45%). And the food allergen positive rate of the age group 1 (aged from 0 to 3 years old) was significant higher than the other two age groups (62.50%). Positive rate for simply inhalant allergen was 39.88%, while positive rate for simply food allergen was 7.55% and mixed allergen was 20.54%. Inhalant allergen was the main allergen of the children with allergic disease aged over 3 years old, while food allergen was the main allergen of the children with allergic disease aged below 3 years old. It was safe, sensible and effective to use Uni CAP anaphylactogen detection system for rapid assay of specific allergens. Source

Ding J.-Y.,General Hospital of Guangzhou Military Command | Wang P.,First Peoples Hospital of Jingmen
Medical Principles and Practice | Year: 2011

Objective: To evaluate the accuracy of detection for screening group A streptococci (GAS) in pediatric clinics using fluorescent in situ hybridization (FISH) and immunochromatographic assay (ICA). Subjects and Methods: A group of 630 children who attended an outpatient clinic with signs and symptoms of acute upper respiratory tract infection were enrolled in this study. Specimens were collected using a double-swab collection device. Culturing methods were employed as the gold standard for comparison. Discordant results (i.e., positive results for FISH or ICA along with negative culture results) were further evaluated by using Todd-Hewitt broth (THB) with subsequent subculture for group A selective streptococcus agar. True-positive or false-positive of GAS was determined by the presence or absence of THB subculture. The diagnostic characteristics of FISH and ICA were assessed. Results: After discrepant analysis, the sensitivity, specificity and positive and negative predictive values were as follows: 89.2, 100, 100 and 95.4%, respectively, for FISH; corresponding values for ICA were 76.8, 98.6, 96.1 and 90.5%. Conclusion: The results demonstrated that the FISH assay had a higher detection sensitivity than ICA and is suitable for rapid and accurate GAS screening in pediatric clinics. © 2011 S. Karger AG, Basel. Source

Wang G.X.,Liaoning Medical University | Hu L.,First Peoples Hospital of Jingmen | Zhang Z.,Liaoning Medical University | Liu D.P.,Liaoning Medical University
Genetics and Molecular Research | Year: 2014

The aim of this study was to construct an adenoviral expression vector for vascular endothelium growth factor 121 (VEGF121)-FLAG and humanized Renilla reniformis green fluorescent protein (hrGFP-1) genes, and to observe their expressions in bone marrow mesenchymal stem cells. Using pTG19T-VEGF121 as a template, polymerase chain reaction technology was adopted to mutate the VEGF121 gene by removing the stop codon and inserting NotI and XhoI restriction sites both before and after the gene sequences. The resultant gene was then subcloned into a pMD19-T plasmid, the pMD19-T-VEGF121 and pShuttle-CMV-IRES-hrGFP-1 plasmids were double-digested, and small and large fragments were linked after gel recovery to complete the construction of recombinant adenovirus vectors. After titer determination, the recombinant adenovirus vectors were used to affect rabbit bone marrow mesenchymal stem cells, and fluorescence intensity was observed under fluorescence microscopy. Enzyme digestion identification and sequencing confirmed that the recombinant plasmids were successfully constructed, and observations under fluorescence microscopy showed significant expression of green fluorescent protein in recombinant adenovirus-infected bone marrow mesenchymal stem cells. The constructed adenoviral gene expression vectors carrying VEGF121-FLAG and hrGFP-1 can be expressed in eukaryotic cells, which may be used for gene therapy of ischemic disorders. © FUNPEC-RP. Source

Xia H.-C.,Ningxia Medical University | Wang F.,First Peoples Hospital of Jingmen | Li Y.-H.,Ningxia Medical University | Li Z.-K.,Ningxia Medical University | And 3 more authors.
Future Neurology | Year: 2012

Per2 plays a key role in regulating the circadian rhythm in mammals. However, the circadian clock gene expression of Per1 and Per2 and its influence on radiotherapeutic sensitivity of C6 glioma cells in vitro have not been explored. Aim: To investigate the rhythm expression of circadian gene Per1 and Per2, and examine the influence on radiotherapeutic sensitivity of two important clock genes in C6 glioma cells. Materials & methods: The cultured C6 glioma cells and NIH3T3 cells were stimulated by phorbol 12-myristate 13-acetate (PMA). The expression of Per1 and Per2 at the indicated times were examined with a method for the absolute quantification of cDNA using real-time PCR. The cultured cell were given x-irradiation at the indicated times and the cell-cycle, apoptosis and proliferation were examined by flow cytometry. Results: We report here that PMA treatment of C6 rat glioma cells induces circadian expression of Per2, and that during periods of high expression, cells are blocked at the G2/M transition and are more sensitive to x-irradiation. PMA treatment of NIH3T3 cells induced circadian expression of Per1 and Per2, but high Per expression did not block the cell cycle or render the cells more sensitive to irradiation. Conclusion: Our results suggest that Per2 expression may increase the efficacy of radiotherapy against glioma. © 2012 Future Medicine Ltd. Source

Cai X.-Y.,Huazhong University of Science and Technology | Xiong L.-M.,Huazhong University of Science and Technology | Yang S.-H.,Huazhong University of Science and Technology | Shao Z.-W.,Huazhong University of Science and Technology | And 3 more authors.
Spine Journal | Year: 2014

Background context It has been shown that bupivacaine, the most commonly used local anesthetic to relieve or control pain in interventional spine procedures, is cytotoxic to intervertebral disc (IVD) cells in vitro. However, some other common local anesthetics, such as ropivacaine and lidocaine, are also frequently used in the treatment of spine-related pain, and the potential effects of these agents remain unclear. Purpose The purpose of this study was to evaluate the effect of various local anesthetics on rabbit IVD cells in vitro and further compare the cytotoxicity of ropivacaine, bupivacaine, lidocaine, and saline solution control. Study design Controlled laboratory study. Subjects Rabbit annulus fibrosus (AF) and nucleus pulposus (NP) cells were isolated from Japanese white rabbits. Methods Both AF and NP cells at the second generation maintained in monolayer were exposed to various concentrations of local anesthetics (eg, bupivacaine) or different durations of exposure and evaluated for cell viability by use of cell counting kit-8 (CCK-8). In addition, to compare the cytotoxicity of ropivacaine, bupivacaine, lidocaine, and saline solution control in commercial concentration, the viability was analyzed by flow cytometry after 60-minute exposure, and the morphologic changes were observed by the phase-contrast microscopy. Apoptosis and necrosis of IVD cells were confirmed by using fluorescence microscopy with double staining of Hoechst 33342 and propidium iodide. Results Rabbit IVD cell death demonstrated a time and dose dependence in response to bupivacaine and lidocaine. However, ropivacaine only exerted a significant time-dependent effect on IVD cells. There was no significant difference in IVD viability after treatment with different doses of ropivacaine. In addition, the results showed that lidocaine was the most toxic of the three local anesthetics and that ropivacaine presented less cytotoxicity than lidocaine and bupivacaine. Fluorescence microscopy also confirmed that the short-term toxic effect of local anesthetics on both AF and NP cells was mainly caused by necrosis rather than apoptosis. Conclusions Results show that bupivacaine and lidocaine decrease cell viability in rabbit IVD cells in a dose- and time-dependent manner. All local anesthetics should be avoided if at all possible. Ropivacaine may be a choice if necessary, but it is also toxic. The increase in cell death is more related with cell necrosis rather than cell apoptosis. If these results can be corroborated in tissue explant models or animal studies, caution regarding diagnosing, treating, and controlling spine-related pain with local anesthetics is prompted. © 2014 Elsevier Inc. All rights reserved. Source

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