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Zhou Y.-F.,First Peoples Hospital of Hangzhou
Hepato-Gastroenterology | Year: 2013

Background/Aims: To systematically evaluate the effect and safety of octreotide on primary moderate to severe acute pancreatitis. Methodology: The Cochrane Central Register of Controlled Trials (CENTRAL) in The Cochrane Library, PubMed, EMBASE, Science Citation Index Expanded (SCI-E), and Chinese Biomedicine Database (CBM) were searched in September 2011. Major outcomes contained mortality, incidence rate of complications, rate of surgical intervention, and length of hospital stay. Results: Eleven randomized clinical trials with 720 participants were included and evaluated, only two of which had a high study quality and were combined in meta-analysis. The pool estimate of RR of mortality was 0.88 (95% CI: 0.53, 1.45) and that of incidence rate of complication was 1.08 (95% CI: 0.94, 1.26), both of which had no significant difference. The other two outcomes could not be combined for lack of enough data. Conclusions: Present evidence does not approve octreotide's benefit in the major outcomes of moderate to severe acute pancreatitis and further randomized controlled trials with high quality and large sample size are required. © H.G.E. Update Medical Publishing S.A.


Yang J.,Zhejiang University | Yang J.,First Peoples Hospital of Hangzhou | Fox M.,University of Zürich | Fox M.,NIHR Nottingham Digestive Diseases Biomedical Research Unit | And 6 more authors.
Alimentary Pharmacology and Therapeutics | Year: 2014

Background Irritable bowel syndrome patients with diarrhoea (IBS-D) often report intolerance to milk; however, the mechanism underlying these symptoms is unknown. Aim To assess the role of psychological factors, immune activation and visceral sensitivity on the development of lactose intolerance (LI) in IBS-D patients. Methods Fifty-five IBS-D patients and 18 healthy controls (HCs) with lactase deficiency underwent a 20-g lactose hydrogen breath test (LHBT). Patients were categorised as lactose malabsorption (LM; malabsorption only) or LI [malabsorption plus increase in total symptom score (TSS). Measurements included (i) psychological status; (ii) enteric biopsies with quantification of mast cells (MCs), T-lymphocytes and enterochromaffin cells; (iii) serum cytokines; (iv) rectal sensitivity before and after lactose ingestion. Results LI was more prevalent in IBS-D patients than HCs [25/55 (46%) vs. 3/18 (17%), P = 0.029]. IBS-D patients with LI had (i) higher levels of anxiety than those with LM (P = 0.017) or HCs (P = 0.006); (ii) increased mucosal MCs compared with LM (P = 0.006) and HCs (P < 0.001); (iii) raised serum TNF-α compared with LM (P = 0.034) and HCs (P < 0.001) and (iv) increased rectal sensitivity after lactose ingestion compared with LM (P < 0.001) or HCs (P < 0.001). Severity of abdominal symptoms after lactose ingestion was associated with the increase in visceral sensitivity after lactose intake (r = 0.629, P < 0.001), MCs (r = 0.650, P < 0.001) and anxiety (r = 0.519, P < 0.001). Conclusions IBS-D patients with lactose intolerence are characterised by anxiety, mucosal immune activation and increased visceral sensitivity after lactose ingestion. The presence of these biomarkers may indicate an IBS phenotype that responds to dietary therapy and/or mast cell stabilisers (ClinicalTrials.gov Identifier: NCT01286597). © 2013 John Wiley & Sons Ltd.


Lou H.-Z.,Zhejiang University | Weng X.-C.,Hangzhou Xia sha Hospital | Pan H.-M.,Zhejiang University | Pan Q.,Zhejiang University | And 3 more authors.
Biochemical and Biophysical Research Communications | Year: 2014

Pancreatic cancer has one of worst prognosis among all human malignancies around the world, the development of novel and more efficient anti-cancer agents against this disease is urgent. In the current study, we tested the potential effect of INK-128, a novel mammalian target of rapamycin (mTOR) complex 1 and 2 (mTORC1/2) dual inhibitor, against pancreatic cancer cells in vitro. Our results demonstrated that INK-128 concentration- and time-dependently inhibited the survival and growth of pancreatic cancer cells (both primary cells and transformed cells). INK-128 induced pancreatic cancer cell apoptosis and necrosis simultaneously. Further, INK-128 dramatically inhibited phosphorylation of 4E-binding protein 1 (4E-BP1), ribosomal S6 kinase 1 (S6K1) and Akt at Ser 473 in pancreatic cancer cells. Meanwhile, it downregulated cyclin D1 expression and caused cell cycle arrest. Finally, we found that a low concentration of INK-128 significantly increased the sensitivity of pancreatic cancer cells to gemcitabine. Together, our in vitro results suggest that INK-128 might be further investigated as a novel anti-cancer agent or chemo-adjuvant for pancreatic cancer treatment. © 2014 Elsevier Inc. All rights reserved.


Yang J.,Zhejiang University | Yang J.,First Peoples Hospital of Hangzhou | Deng Y.,Zhejiang University | Chu H.,Zhejiang University | And 8 more authors.
Clinical Gastroenterology and Hepatology | Year: 2013

Background & Aims: The effects of lactase deficiency on digestive symptoms and diet in patients with irritable bowel syndrome (IBS) have not been well defined. We assessed lactose absorption and tolerance and the intake of dairy products in healthy volunteers (controls) and patients with diarrhea-predominant IBS (D-IBS). Methods: Sixty patients diagnosed with D-IBS at the Sir Run Run Shaw Hospital, Hangzhou, China and 60 controls were given hydrogen breath tests to detect malabsorption and intolerance after administration of 10, 20, and 40 g lactose in random order 7-14 days apart; participants and researchers were blinded to the dose. We assessed associations between the results and self-reported lactose intolerance (LI). Results: Malabsorption of 40 g lactose was observed in 93% of controls and 92% of patients with D-IBS. Fewer controls than patients with D-IBS were intolerant to 10 g lactose (3% vs 18%; odds ratio [OR], 6.51; 95% confidence interval [CI], 1.38-30.8; P = .008), 20 g lactose (22% vs 47%; OR, 3.16; 95% CI, 1.43-7.02; P = .004), and 40 g lactose (68% vs 85%; OR, 2.63; 95% CI, 1.08-6.42; P = .03). H2 excretion was associated with symptom score (P = .001). Patients with D-IBS self-reported LI more frequently than controls (63% vs 22%; OR, 6.25; 95% CI, 2.78-14.0; P < .001) and ate fewer dairy products (P = .040). However, self-reported LI did not correlate with results from hydrogen breath tests. Conclusions: The risk of LI is related to the dose of lactose ingested and intestinal gas production and is increased in patients with D-IBS. Self-reported LI, but not objective results from hydrogen breath tests, was associated with avoidance of dairy products. © 2013 AGA Institute.


Duan X.,First Peoples Hospital of Hangzhou | Duan X.,Peoples Hospital of Aksu County
Journal of Electrocardiology | Year: 2014

A case of electrocardiographic artifact due to mobile a phone mimicking ventricular tachycardia was presented. The artifact was discriminated by close scrutiny of ECG and was attributed to a mobile phone because it was simultaneous with mobile phone game. © 2014 Elsevier Inc. All rights reserved.


Kong F.-J.,First Peoples Hospital of Hangzhou | Ma L.-L.,Zhejiang University | Hu W.-W.,Zhejiang University | Wang W.-N.,Zhejiang University | And 2 more authors.
Biochemical Pharmacology | Year: 2012

We developed a maternal fetal rat model to study the effects of isoflurane-induced neurotoxicity on the fetuses of pregnant rats exposed in utero. Pregnant rats at gestational day 14 were exposed to 1.3 or 3% isoflurane for 1 h. At postnatal day 28, spatial learning and memory of the offspring were examined using the Morris Water Maze. The apoptosis was evaluated by caspase-3 immunohistochemistry in the hippocampal CA1 region. Simultaneously, the ultrastructure changes of synapse in the hippocampal CA1 and dentate gyrus region were observed by transmission electron microscopy (TEM). The 3% isoflurane treatment group showed significantly longer escape latency, less time spent in the third quadrant and fewer original platform crossings in the Morris Water Maze test, significantly increased number and optical densities of caspase-3 neurons. This treatment also produced remarkable changes in synaptic ultrastructure compared with the control and the 1.3% isoflurane groups. There were no differences in the Morris Water Maze test, densities of caspase-3 positive cells, or synaptic ultrastructure between the control and 1.3% isoflurane groups. High isoflurane concentration (3%) exposure during pregnancy caused spatial memory and learning impairments and more neurodegeneration in the offspring rats compared with control or lower isoflurane concentrations. © 2012 Elsevier Inc.


Yu Y.-P.,First Peoples Hospital of Hangzhou | Huang X.-M.,First Peoples Hospital of Hangzhou
Chinese Journal of Contemporary Pediatrics | Year: 2012

This paper provides an overview of the current state of pharmacotherapy in children with pulmonary arterial hypertension (PAH) and a brief introduction to the potentially novel pharmacologic targets for PAH. Currently, 3 classes of drugs including prostacyclin analogues, endothelin receptor antagonists and phosphodiesterase-5 inhibitors are approved for the treatment of PAH in children, which has led to improved hemodynamics, increased exercise capacity and prolonged survival. Despite these improvements, there is still a need to carry out well-designed, randomized, controlled studies with larger samples. In addition, novel drugs targeting other molecular pathways should be developed.


Sun W.-C.,First Peoples Hospital of Hangzhou | Li W.,First Peoples Hospital of Hangzhou | Chen Q.-H.,First Peoples Hospital of Hangzhou | Tong J.-Y.,First Peoples Hospital of Hangzhou
Journal of Obstetrics and Gynaecology Research | Year: 2013

Corpus luteum hemorrhage may lead to a life-threatening intraperitoneal hemorrhage in women with bleeding disorders. A 17-year-old girl with aplastic anemia presented to the emergency ward with complaints of severe abdominal pain for the last 24 h. On examination, she was noted to be pale with circulatory compromise. Transabdominal pelvic ultrasonography revealed a left complex adnexal mass, 6.9 + 5.1 + 5.1 cm3 in size, with a large anechoic area of fluid in the abdomen. On laparoscopic exploration, there was a massive hemoperitoneum (2.6 L) with a ruptured corpus luteum on the surface of the left ovary. Hemostatic electric coagulationwas successfully applied to the bleeding surface. The patient recovered without incident and was transferred to a special hospital for blood disease treatment one week after surgery. We concluded that corpus luteum hemorrhage in women with aplastic anemia can cause therapeutic difficulties. Laparoscopy is feasible for the management of a massive intraperitoneal hemorrhage secondary to a ruptured corpus luteum in such patients. © 2012 The Authors.


Jin X.,Zhejiang University | Lin Z.,First Peoples Hospital of Hangzhou | Xie X.,Zhejiang University
European Journal of Clinical Microbiology and Infectious Diseases | Year: 2010

Keratitis caused by Pseudomonas aeruginosa is a potentially vision-threatening condition that requires prompt treatment to prevent vision loss. The recognition of infectious agents by the Toll-like receptor (TLR) system initiates primary innate and later adaptive immune responses. In this study, in late cases of corneal P. aeruginosa infection, the expression of TLR2, 4, 5 and 9 mRNA were all upregulated. In early infection cases, only TLR9 mRNA expression was upregulated. In late cases, the protein expression of TLR2, 4, 5, 9 and pIκB-α were elevated. In early cases, only TLR9 and pIκB-α expression were upregulated. Concentrations of IL-6 and IL-8 increased in infected corneas, especially in late cases. Myeloperoxidase (MPO) activity suggested that polymorphonuclear leukocyte (PMN) numbers were higher in late than in early stages of infection. The delayed response of TLRs may explain why P. aeruginosa infection exacerbates rapidly at the early infection stage. This finding may have important implications for the treatment of innate immunologic responses to corneal infections. © 2009 Springer-Verlag.


Duan X.,First Peoples Hospital of Hangzhou | Ling F.,First Peoples Hospital of Hangzhou | Shen Y.,First Peoples Hospital of Hangzhou | Yang J.,First Peoples Hospital of Hangzhou | Xu H.-Y.,First Peoples Hospital of Hangzhou
Europace | Year: 2012

AimsContrast-guided axillary vein puncture is commonly used for pacemaker or defibrillator lead implantation. Venous spasm during contrast-guided axillary vein puncture has been reported rarely. We investigated the incidence of venous spasm during contrast-guided axillary vein puncture and the impact of venous spasm on axillary vein puncture.Methods and resultsSeventy-four consecutive patients referred for contrast-guided axillary vein puncture for pacemaker or defibrillator implantation were included in the study. The puncture was guided by uoroscopy and the venogram of axillary vein. After the success of puncture, the venography was taken again. When the puncture could not be successful up to 3 min, the second venography was taken and the puncture was guided by the second venogram. The degree of venous spasm was evaluated by the reduction in the lumen calibre of the axillary vein after puncture. Mild venous spasm and severe venous spasm were defined as a reduction in the lumen calibre of 5090 and <90, respectively. The success rate of axillary vein puncture within 3 min was 87.8, and the total success rate was 95.9. Mild venous spasm occurred in 22 patients (29.7) and severe venous spasm occurred in 6 patients (8.1). Severe venous spasm occurred in all the three patients with a failed puncture. Severe venous spasm was independently negatively correlated with both success rate within 3 min and total success rate.ConclusionVenous spasm is not a rare phenomenon during the contrast-guided puncture of axillary vein and it has an important negative impact on the puncture. Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2012.2012 © Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2012.

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