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Wang Y.-Z.,Central South University | Wang Y.-Z.,Affiliated Hospital of Jining Medical College | Tian F.-F.,Central South University | Yan M.,The First Peoples Hospital of Foshan City | And 6 more authors.
Clinical and Experimental Immunology | Year: 2014

Summary: MicroRNA-155 (miR155) is required for antibody production after vaccination with attenuated Salmonella. miR155-deficient B cells generated reduced germinal centre responses and failed to produce high-affinity immunoglobulin (Ig)G1 antibodies. In this study, we observed up-regulation of miR155 in the peripheral blood mononuclear cells (PBMCs) of patients with myasthenia gravis (MG), and miR155 was also up-regulated in torpedo acetylcholine receptor (T-AChR)-stimulated B cells. We used an inhibitor of miR155 conjugated to anti-CD20 single-chain antibody to treat both the cultured B cells and the experimental autoimmune MG (EAMG) mice. Our results demonstrated that silencing of miR155 by its inhibitor impaired the B cell-activating factor (BAFF)-R-related signalling pathway and reduced the translocation of nuclear factor (NF)-κB into the nucleus. Additionally, AChR-specific autoantibodies were reduced, which may be related to the altered amounts of marginal zone B cells and memory B cells in the spleens of EAMG mice. Our study suggests that miR155 may be a promising target for the clinical therapy of MG. © 2014 British Society for Immunology.

Liu C.,The First Peoples Hospital of Foshan City | Zhang P.,The First Peoples Hospital of Foshan City | Rong X.,The First Peoples Hospital of Foshan City
Chinese Journal of Rehabilitation Medicine | Year: 2011

Objective: To observe the effect of low-frequency electrical pulse stimulation on foot drop patients after stroke. Method: Based on routine medical treatment and rehabilitation therapy, 30 patients with foot drop after stoke received electrical stimulation on common peroneal nerve and tibialis anterior muscle of affected side by using XFT-2001 low-frequency electrical pulse stimulator twice a day. In one day, at the first time only exercise mode was used as neuromuscular electrical stimulation (NEMS) on affected tibialis anterior muscle for 20min; at the second time the same NMES and 15-20min walking training were proceeded, 5-6d per week, 2 weeks in total. Before treatment, XFT-2001P neuromuscular locator was used to find sensitive position, then the negative electrode was placed on sensitive position (common peroneal nerve) and the positive electrode was placed on the suitable position (tibialis anterior muscle). Walking function was evaluated before treatment, 1 week after treatment and 2 weeks after treatment. Result: After 2 weeks treatment, daring walking with low-frequency electrical pulse stimulator walking speed, transfer ability, up and down stairs ability improved immediately and the physiological cost consumption of foot drop patients after stroke reduced (P<0.05-0.001). Conclusion: Low-frequency electrical pulse stimulator could improve walking function of affected low limbs of foot drop patients after stroke. Walking speed, transfer ability, up and down stairs ability improved significantly and physiological consumption reduced significantly during walking with the stimulator. The stimulator had no obviaus side effect and it was considered to be a safe and effective training equipment.

Cai S.,Sun Yat Sen University | Chen R.,Sun Yat Sen University | Li X.,Sun Yat Sen University | Cai Y.,Sun Yat Sen University | And 15 more authors.
Oncotarget | Year: 2015

Here we found that levels of miR-23a were decreased in prostate cancer cell lines and tumor tissues. These low levels were associated with poor patients' prognosis. MiR-23a inhibited migration and invasion of prostate cancer in vivo and in orthotopic prostate cancer mice model. MiR-23a decreased levels of p21-activated kinase 6 (PAK6). Expression of miR-23a inhibited phosphorylation of LIM kinase 1 (LIMK1) and cofilin, in turn suppressing formation of stress fibers and actin filaments, which was required for cell motility and invasion. PAK6 bound to LIMK1 and activated it via phosphorylation at Thr-508. Also, PAK6 and LIMK1 were colocalized in the cytoplasma. Thus, miR-23a regulated cytoskeleton by affecting LIMK1 and cofilin. In summary, we have identified the miR-23a-PAK6-LIMK1 pathway of prostate cancer metastasis. Potential therapeutic approach by targeting miR-23 is suggested.

Wang P.-p.,Sun Yat Sen University | Xie D.-y.,Sun Yat Sen University | Liang X.-J.,Jinan University | Peng L.,Sun Yat Sen University | And 4 more authors.
PLoS ONE | Year: 2012

Aims: Bone marrow-derived mesenchymal stem cells (BMSCs) can reduce liver fibrosis. Apart from the paracrine mechanism by which the antifibrotic effects of BMSCs inhibit activated hepatic stellate cells (HSCs), the effects of direct interplay and juxtacrine signaling between the two cell types are poorly understood. The purpose of this study was to explore the underlying mechanisms by which BMSCs modulate the function of activated HSCs. Methods: We used BMSCs directly and indirectly co-culture system with HSCs to evaluate the anti-fibrosis effect of BMSCs. Cell proliferation and activation were examined in the presence of BMSCs and HGF. c-met was knockdown in HSCs to evaluate the effect of HGF secreted by BMSCs. The TLR4 and Myeloid differentiation primary response gene 88(MyD88) mRNA levels and the NF-kB pathway activation were determined by real-time PCR and western blotting analyses. The effect of BMSCs on HSCs activation was investigated in vitro in either MyD88 silencing or overexpression in HSCs. Liver fibrosis in rats fed CCl4 with and without BMSCs supplementation was compared. Histopathological examinations and serum biochemical tests were compared between the two groups. Results: BMSCs remarkably inhibited the proliferation and activation of HSCs by interfering with LPS-TLR4 pathway through a cell-cell contact mode that was partially mediated by HGF secretion. The NF-kB pathway is involved in HSCs activation inhibition by BMSCs. MyD88 over expression reduced the BMSC inhibition of NF-kB luciferase activation. BMSCs protected liver fibrosis in vivo. Conclusion: BMSCs modulate HSCs in vitro via TLR4/MyD88/NF-kB signaling pathway through cell-cell contact and secreting HGF. BMSCs have therapeutic effects on cirrhosis rats. Our results provide new insights into the treatment of hepatic fibrosis with BMSCs. © 2012 Wang et al.

Zheng X.,The First Peoples Hospital of Foshan City | Zhao G.-P.,The First Peoples Hospital of Foshan City | Li S.-X.,The First Peoples Hospital of Foshan City | Yu J.-H.,The First Peoples Hospital of Foshan City | Hu D.,The First Peoples Hospital of Foshan City
International Eye Science | Year: 2013

AIM: To explore the relations of clinical efficacy and surgical timing of vitrectomy combined with silicone oil tamponade for severe infectious traumatized endophthalmitis. METHODS: Totally 59 patients (59 eyes) with severe infectious traumatized endophthalmitis accepted vitrectomy combined with silicone oil tamponade. Patients were divided into two groups by different surgical timing. Group A accepted operation in 24 hours. Group B accepted operation 24 hours after injury. Retina status during operation, clinical efficacy and best-corrected visual acuity were observed and recorded. RESULTS: The cases of early operation group got lesser retina injury and higher efficacy and better best-corrected visual acuity. CONCLUSION: Vitrectomy combined with silicone oil tamponade is an effective way to cure severe infected traumatized endophthalmitis. Early surgical treatment is the key to achieve better effect.

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