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Liu K.-F.,Xiangnan College | Feng S.-Y.,Nanhua University | Zhou X.-P.,First Peoples Hospital of Chenzhou City | Kuang G.-P.,First Peoples Hospital of Chenzhou City
International Journal of Ophthalmology | Year: 2010

• AIM: To observe the clinical effect of phacoemulsification and intraocular lens on patients with angle-closure glaucoma at preclinical or presymptomatic stage. • METHODS: Sixty-six patients (66 eyes) with acute angle-closure glaucoma at preclinical or presymptomatic stage were chosen and randomly divided into three groups, with 22 eyes in each group. Group A received laser peripheral iridotomy. Group B received phacoemulsification and intraocular lens. Group C received phacoemulsification and intraocular lens combined with peripheral iridotomy. The changes of intraocular pressure, anterior chamber angle, corrected vision and anterior chamber depth were examined during the 11.72 ± 0.96 months follow-up. • RESULTS: The results in the final follow-up after operation were as follows: the anterior chamber angle in group A, group B and group C widened significantly(P < 0.05), the difference in the gonioscopy grading of the anterior chamber angle among the three groups was not statistically significant. The anterior chamber depth in Group A did not change significantly, the anterior chamber depth significantly increased in group B and group C(P < 0.05), the anterior chamber depth in group A was lower than that in group B and group C, and the difference was statistically significant (P < 0.05), the difference between group B and group C was not statistically significant. The intraocular pressure in the three groups did not change significantly, the variance in the intraocular pressure among the three groups was not statistically significant. The sensitivity of dark room provocative test in group A did not change significantly, the sensitivity of dark room provocative test in group B and group C significantly decreased (P < 0.05), the difference among the three groups was not statistically significant. The corrected vision in group A did not change significantly, the corrected vision in group B and group C improved significantly (P < 0.05). • CONCLUSION: Phacoemulsification and intraocular lens is superior to laser peripheral iridotomy on the aspects of widening the anterior chamber angle, reducing potentialy the sensitivity of dark room provocative test and improving the corrected vision. It is not necessary to combine peripheral iridotomy with phacoemulsification and intraocular lens.


Luo D.-X.,Central South University | Luo D.-X.,University of South China | Luo D.-X.,First Peoples Hospital of Chenzhou City | Xia C.-L.,University of South China | And 9 more authors.
Biochemical and Biophysical Research Communications | Year: 2010

Objective: To investigate the effect of static pressure on cholesterol accumulation in vascular smooth muscle cells (VSMCs) and its mechanism. Methods: Rat-derived VSMC cell line A10 treated with 50. mg/L ox-LDL and different static pressures (0, 60, 90, 120, 150, 180. mm. Hg) in a custom-made pressure incubator for 48. h. Intracellular lipid droplets and lipid levels were assayed by oil red O staining and HPLC; The mRNA levels of caveolin-1 and ABCA1, the protein levels of caveolin-1 SREBP-1 and mature SREBP-1 were respectively detected by RT-PCR or western blot. ALLN, an inhibitor of SREBP metabolism, was used to elevate SREBP-1 protein level in VSMCs treated with static pressure. Results: Static pressures significantly not only increase intracellular lipid droplets in VSMCs, but also elevate cellular lipid content in a pressure-dependent manner. Intracellular free cholesterol (FC), cholesterol ester (CE), total cholesterol (TC) were respectively increased from 60.5 ± 2.8. mg/g, 31.8 ± 0.7 mg/g, 92.3 ± 2.1 mg/g at atmosphere pressure (ATM, 0 mm Hg) to 150.8 ± 9.4 mg/g, 235.9 ± 3.0 mg/g, 386.7 ± 6.4 mg/g at 180 mm. Hg. At the same time, static pressures decrease the mRNA and protein levels of caveolin-1, and induce the activation and nuclear translocation of SREBP-1. ALLN increases the protein level of mature SREBP-1 and decreases caveolin-1 expression, so that cellular lipid levels were upregulated. Conclusion: Static pressures stimulate ox-LDL-induced cholesterol accumulation in cultured VSMCs through decreasing caveolin-1 expression via inducing the maturation and nuclear translocation of SREBP-1. © 2010 Elsevier Inc.


Luo D.-X.,Central South University | Luo D.-X.,University of Illinois at Springfield | Luo D.-X.,University of South China | Peng X.-H.,First Peoples Hospital of Chenzhou City | And 6 more authors.
Molecular and Cellular Biochemistry | Year: 2011

Insulin-like growth factor-1 (IGF-1) plays the role in cellular lipid synthesis and cell proliferation. However, the role of IGF-1 on the growth of colon cancer cell line HCT-8 is not clear. In this study, HCT-8 cells were exposed to IGF-1 at 0, 10, 50, or 100 ng/ml in serum-free medium. Fatty acid/lipid synthesis in HCT-8 cells was examined by 2-14C-acetate incorporation. HCT-8 cell growth and proliferation were determined by MTT assay and Trypan blue exclusive viable cell counting. We found that in serum starvation conditions, IGF-1 at 10-100 ng/ml induced dose-dependent down regulation of both the ACCα expression and the phosphorylation in HCT-8 cells, maintaining a balance in ACCα activity and lipid synthesis. IGF-1 reduced p-ATM, p-AMPK, and then p-ACCα protein levels in HCT-8 cells. IGF-1 increased p-Akt levels, but decreased p-ERK1/2 levels, leading to the decrease in ACCα protein and mRNA levels. Similarly, ERK1/2 inhibitor PD98059 reduced ACCα expression. IGF-1 influences neither HCT-8 cell growth nor their p53 protein levels and PARP cleavage. In a word, IGF-1 reduced ACCα phosphorylation via an ATM/AMPK signaling pathway and suppressed ACCα expression through an ERK1/2 transduction, playing a dual role in regulating ACCα activity and lipogenesis. This may render a cell with survival advantages under a serum starvation crisis, representing a novel mitogenic role of IGF-1. © Springer Science+Business Media, LLC. 2011.


Xie A.,First Peoples Hospital of Chenzhou City | Fang C.,Southern Medical University | Huang Y.,Southern Medical University | Fan Y.,Southern Medical University | And 2 more authors.
Journal of Gastroenterology and Hepatology (Australia) | Year: 2013

Background and Aim: Hepatolithiasis often requires repeated operations in East Asia. This study aims to evaluate the clinical application of three-dimensional reconstruction and visible simulation techniques for repeated operation in patients with intrahepatic calculi. Methods: A medical image processing system was used for modeling, segmentation, and three-dimensional reconstruction of intrahepatic stones in 20 patients, consisting of 7 males and 13 females who were subjected to repeated surgical treatment from May 2010 to November 2011. The three-dimensional models of the liver and bile ducts in a standard template library format were then processed by the FreeForm Modeling System. Accurate digital information about the bile duct system, lesions, calculi distribution, and surrounding organs obtained from all directions, multiple angles, and multistrata were used to decide the rational surgical modality. Virtual operations were then performed on the models with virtual surgical instruments in the FreeForm Modeling System. The results were used to guide and were compared with the real surgical procedures performed. Results: The surgical outcomes of all patients in this study were satisfactory. Three-dimensionally reconstructed models provided clear and strong relief perception and a user-friendly interface. Visible simulation surgery performed based on three-dimensionally reconstructed models led to an optimal operation plan that had great resemblance to the actual surgeries for cases with intrahepatic stones. Conclusions: Three-dimensional reconstruction and visible simulation techniques had unique value in optimizing repeated operation plans and in guiding actual surgical procedures for patients with recurrent intrahepatic calculi. © 2012 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.


Jian D.,Central South University | Jiang D.,Hunan Research Center for Safety Evaluation of Drug | Su J.,Central South University | Chen W.,Central South University | And 5 more authors.
Steroids | Year: 2011

Background: It is well known that melanin synthesis in melanoma cells is controlled by melanogenic enzymes, which regulate the cAMP-PKA signaling pathway. Estrogen was previously reported to upregulate melanogenesis that is associated with human skin pigmentation. Objective: To investigate the influence and mechanism of diethylstilbestrol (DES) on melanogenesis in mouse B16 melanoma cells. Methods: The effects of diethylstilbestrol on cell viability, melanin content, tyrosinase activity, cAMP level, expression of the tyrosinase family and microphthalmia related transcription factor (MITF) were measured in B16 melanoma. Estrogen receptor (ER) expression were detected in B16 melanoma and A375 melanoma. Diethylstilbestrol-induced melanin synthesis were evaluated in the presence and absence of H89 (a PKA-specific inhibitor) and ICI182, 780 (a pure ER antagonist). Tyrosinase activity, the mRNA levels of tyrosinase and MITF were evaluated in the presence and absence of H89. Results: In B16 cells, diethylstilbestrol increased cell proliferation, melanin synthesis, tyrosinase activity and expression of the tyrosinase family and MITF. ER expression have not difference in human and mouse melanoma. When ER were inhibited by ICI182, 780, DES-induced melanogenesis was significantly reduced. Diethylstilbestrol enhanced the level of cAMP. The upregulation of melanin content and tyrosinase activity stimulated by diethylstilbestrol was significantly attenuated in the presence of H89. Further, diethylstilbestrol-induced upregulation of tyrosinase and MITF were significantly attenuated when the PKA pathway was blocked. Conclusions: Diethylstilbestrol can enhance melanin synthesis in melanoma cells. This effect is associated with activation of the cAMP-PKA pathway and upregulation of expression and activity of the melanogenesis-related enzyme tyrosinase and MITF. © 2011 Elsevier Inc. All rights reserved.


Wen H.,Kunming Medical University | Wen H.,First Peoples Hospital of Chenzhou City | Chen Y.,Huazhong University of Science and Technology | Hu Z.,Kunming Medical University | And 4 more authors.
Oncology Reports | Year: 2014

BATF2, also called SARI, is associated with several cancer types, and loss of BATF2 expression is frequently detected in aggressive and metastatic cancers. The expression of BATF2 was previously shown to slow the growth rate of malignant tumor cells injected into athymic nude mice, and decreased expression of BATF2 has been correlated to poor prognosis in hepatocellular carcinoma. However, the func tional role of BATF2 in oral tongue squamous cell carcinoma (OTSCC) remains unknown. In the present study, we examined BATF2 expression in 16 fresh, paired OTSCC and adjacent non-tumor tissues, as well as in a normal tongue epithelial cell line and in 5 OTSCC cell lines by quantitative PCR and western blot analysis. We also evaluated BATF2 expression in 202 paraffin-embedded OTSCC and 30 adjacent non-tumor samples by immunohistochemistry, and its relationship with clinicopathological features and prognosis was investigated. We found that BATF2 expression was significantly reduced in the majority of the 16 OTSCC tumor tissues and the 5 OTSCC cell lines when compared with the non-tumor tissues and the normal tongue epithelial cell line, respectively. Consistent with these results, our immunohistochemistry analysis revealed that decreased BATF2 expression was present in 124 of the 202 cases and was significantly correlated with poor tumor differentiation (P=0.002). Patients with decreased BATF2 expression showed reduced survival when compared to those with high expression (P<0.001). Multivariate analysis revealed that BATF2 expression is an independent predictor of overall survival (P=0.001). These results demonstrate that BATF2 plays a tumor-suppressor role in the development of OTSCC and that BATF2 may serve as a prognostic biomarker and potential therapeutic target for this disease.


Zhang X.,Tianjin University of Traditional Chinese Medicine | Li G.,Tianjin University of Traditional Chinese Medicine | Li G.,First Peoples Hospital of Chenzhou City | Guo L.,Tianjin University of Traditional Chinese Medicine | And 4 more authors.
Neurological Sciences | Year: 2013

Cerebrovascular dysfunction is an early pathogenic event in Alzheimer's disease (AD) and plays a key role in the disease process. Cerebral hypoperfusion, brain glucose hypometabolism and disrupted blood-brain barrier (BBB) integrity contributed to the onset and progression of AD. However, the relationships between the age-related cognitive impairment and cerebral blood flow (CBF), energy metabolism and BBB have not been clearly explained. In this study, we investigated the cognitive function, CBF, BBB damage and expression level of glucose transporter (GLUT) 1 and 3 of senescence-accelerated mouse prone 8 (SAMP8), and the correlations between each of them were analyzed. When compared with SAMR1 (senescence-accelerated mouse resistant 1), the cognitive abilities of SAMP8 were damaged apparently even at 4 months of age, showing up a slower and more capricious acquisition in Morris water maze tasks. In both SAMP8 and SAMR1, reduced CBF and increased BBB leakage were observed with increasing age, but an earlier and more severe impairment was detected in SAMP8. In addition, alterations of GLUT1 and GLUT3 protein expression in cortex and hippocampus were more prominent in SAMP8. Correlation analysis demonstrated that the increased escape latency was correlated negatively with CBF and expression of glucose transporters; and positively with BBB permeability in the hippocampus. These results suggested that CBF, BBB integrity, the expression of GLUT1 and GLUT3 were significantly affected by age and strain, which were also closely associated with cognitive ability. The alteration in CBF and energy failure induced by aging and vascular insults resulted in cognitive decline in SAMP8. © 2013 Springer-Verlag Italia.


Xiao C.-Q.,First Peoples Hospital of Chenzhou City | Chen R.,First Peoples Hospital of Chenzhou City | Lin J.,First Peoples Hospital of Chenzhou City | Wang G.,Central South University | And 3 more authors.
Xenobiotica | Year: 2012

To determine the effect of genistein on cytochrome P450 3A (CYP3A) and P-glycoprotein (P-gp) function using the probe substrates midazolam and talinolol, respectively. Eighteen healthy adult male participants were enrolled in a two-phase randomized crossover design. In each phase, the participants received placebo or genistein for 14 days. On the 15th day, midazolam and talinolol were administered and blood samples were obtained. Midazolam and talinolol pharmacokinetic parameter values were calculated and compared before and after genistein administration. Co-administration of genistein decreased the area under the concentrationtime curve from 0 to 36h (AUC 0-36) (143.65±55.40ng h/mL versus 126.10±40.14ng h/mL, p<0.05), and the area under the concentrationtime curve from zero to infinity (AUC 0-∞) (209.18±56.61ng h/mL versus 180.59±43.03ng h/mL, p<0.05), and also maximum concentration (Cmax) of midazolam (48.86±20.21ng/mL versus 36.25±14.35ng/mL p<0.05). Similarly, AUC 0-36 (2490.282±668. 79ng h/mL versus 2114.46±861.11ng h/mL, p<0.05), AUC 0-∞ (2980.45±921.09ng h/mL versus 2626.92±1003.78ng h/mL, p<0.05) and Cmax of talinolol (326.58±197.67ng/mL versus 293.42±127.19ng/ mL, p<0.05) were reduced by genistein co-administration. The oral clearance of midazolam (1.68±0.85 h-1 versus 3.98±0.59 h-1, p<0.05) and talinolol (3.34±1.24 h-1 versus 3.79±1.55 h-1, p<0.05) were increased by genistien significantly. Administration of genistein can result in a modest induction of CYP3A and possibly P-gp activity in healthy volunteers. © 2012 Informa UK, Ltd.


Li J.,Central South University | Jian D.,Central South University | Chen X.,Central South University | Babajee K.,Central South University | And 3 more authors.
Journal of Dermatological Treatment | Year: 2014

Objectives: The β-blocker propranolol was discovered to be highly effective for the treatment of infantile hemangiomas (IHs), since 2008. Although some side effects have been reported earlier, no serious side effects of its use have been reported so far in Asia, especially in China. To determine the safety of this therapy, the side effects were analyzed in 97 infants who used propranolol (2 mg kg-1·d-1) against hemangioma from 2010 to 2011. Materials and methods: Routine blood and urine tests, hepatic and renal function tests, myocardial enzyme, electrolytes and blood sugar levels at baseline were performed. Electrocardiogram monitoring was performed 48 h after administration of the first dose (2 mg kg-1·d-1). Every patient (n = 97) was required to report to the hospital once a month. Results: The following adverse effects were observed: bronchial hyperactivity (n = 5), cyanosis and cold extremities (n = 1), agranulocytosis (n = 1), and low body temperature (n = 1). These side effects were reported for the first time in Asia. Conclusions: Although propranolol is effective against IHs, its potential side effects should be considered and appropriate monitoring performed. Further studies need to be conducted to determine the optimal dose and duration of propranolol treatment for large and complex hemangiomas. © 2014 Informa Healthcare USA on behalf of Informa UK Ltd.


Liu R.,Central South University | Liu R.,First Peoples Hospital of Chenzhou City | Zhang X.-H.,Central South University | Zhang K.,Central South University | And 4 more authors.
International Journal of Ophthalmology | Year: 2013

AIM To investigate the effect of 5-Aza-2′-deoxy cytidine (5-Aza-CdR), a DNA methyltransferase (DNMT) inhibitor, on the growth and survival of the Chinese retinoblastoma (RB) cell line HXO-RB44. METHODS The DNA methylation status of the Ras association domain family (RASSF1 A) promoter in the presence of 5-Aza-CdR at different concentrations was analyzed by methylation-specific polymerase chain reaction (MSP). RASSF1A m RNA and protein levels were measured by semiquantitative RT-PCR and immunohistochemistry staining, respectively, when cells were treated with 5.0 μmol/L of 5-Aza-CdR. The effect of 5.0 μmol/L 5-Aza-CdR on the proliferation and viability of HXO-RB44 cells was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. RESULTS 5-Aza-CdR efficiently induced cell cycle arrest at G0/G1 and apoptotic death in HXO-RB44 cells. MSP analysis showed that unmethylated RASSF1 A DNA increased and methylated RASSF1 A decreased in a dose-dependent manner in a range of 0.5-5.0 μmol/L 5-Aza-CdR. Accordingly, RASSF1 A expression was reactivated at both m RNA and protein levels. Incubation time of 5-Aza-CdR treatment also functioned as a factor for the demethylation status of RASSF1 A promoter DNA, with a plateau on day four. 5-Aza-CdR at 5.0μmol/L completely demethylated the RASSF1 A promoter in HXO-RB44 cells on day four, and as a result, RASSF1 A expression increased significantly from day 4 to day 7. CONCLUSION 5-Aza-CdR inhibits the growth of the HXO-RB44 RB cell line and induces apoptosis by demethylating the RASSF1 A gene. © Copyright International Journal of Ophthalmology Press.

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