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Chen C.,Nanjing Medical University | Chen C.,First Peoples Hospital of Changzhou | Qian Y.,Nanjing Medical University
Folia Neuropathologica | Year: 2016

Unmethylated CpG DNA, as a stimulatory molecule, has potent pro-inflammatory effects in the central nervous system (CNS). Dexmedetomidine (DEX) has been confirmed to exert anti-inflammatory effects in CNS. Our study was aimed to explore the effects of DEX on tumor necrosis factor-α (TNF-α) expression in unmethylated CpG DNA-challenged microglia. In vivo, after 3 d intracisternal injection of ODN1668, we evaluated the severity of meningitis with or without DEX via pathobiology method and detected the expression of TNF-α from molecular and protein levels. In vitro, we explored whether the ODN1668 could activate microglia to express TNF-α and the inhibition mechanism of DEX. Our results demonstrated that DEX could alleviate the severity of ODN1668-induced meningitis. And while BV2 microglia was stimulated by ODN1668 for different time, TNF-α was increased in mRNA and protein levels but the effect was attenuated by DEX via decreasing phosphorylated AKT and ERK.


Huang Y.,University of Macau | Hu N.,University of Macau | Hu N.,First Peoples Hospital of Changzhou | Gao X.,University of Macau | And 4 more authors.
Chemico-Biological Interactions | Year: 2015

Down-regulation of some hepatic cytochrome P450s (CYP450s) was observed in patients and animals with ulcerative colitis (UC). This study examined changes of CYP450s activities in microsomes of liver (RLMs), intestine (RIMs) and kidney (RRMs) from rats with experimental acute colitis induced by 5% dextran sulfate sodium (DSS) for 7 days and those receiving DSS treatment followed by 7-d cessation through measuring 6α-(CYP1A1), 7α-(CYP2A1), 16α-(CYP2C11) and 2β-/6β-(CYP3A2) hydroxytestosterone (OHT) formed from testosterone. Both pro-(IL-1β, IL-6, TNF-α) and anti-(IL-4, IL-10) inflammatory cytokines were elevated in acute colitis, while the production of the former was enhanced and that of the latter declined by DSS withdrawal. In RLMs, the CYP2A1 activity was significantly increased at DSS stimulation and partially returned to normal level when DSS treatment was terminated. Activity of other CYP450s were decreased by acute colitis and remained after DSS withdrawal. In RRMs, formations of 6α-, 16α- and 2β-OHT significantly declined in acute colitis and DSS termination further potentiated the down-regulation, while 7α-OHT formation was suppressed at DSS stimulation and remained after DSS withdrawal. The formation of 6β-OHT only showed significant decrease after DSS withdrawal. Two metabolites (6α- and 6β-OHT) formed in RIMs and 6β-OHT formation was significantly decreased by DSS stimulation and continued after DSS treatment halted. These findings indicate that the alterations of CYP450s activities vary with organ, CYP isoforms and colitis status, which arouse cautions on efficacy and toxicity of drug therapy during disease progression. © 2015 Elsevier Ireland Ltd.


Wu J.,Soochow University of China | Wu J.,First Peoples Hospital of Changzhou | Xue K.,Shanghai JiaoTong University | Li H.,Shanghai JiaoTong University | And 2 more authors.
PLoS ONE | Year: 2013

Polymer scaffold systems consisting of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) have proven to be possible matrices for the three-dimensional growth of chondrocyte cultures. However, the engineered cartilage grown on these PHBV scaffolds is currently unsatisfactory for clinical applications due to PHBV's poor hydrophilicity, resulting in inadequate thickness and poor biomechanical properties of the engineered cartilage. It has been reported that the incorporation of Bioglass (BG) into PHBV can improve the hydrophilicity of the composites. In this study, we compared the effects of PHBV scaffolds and PHBV/BG composite scaffolds on the properties of engineered cartilage in vivo. Rabbit articular chondrocytes were seeded into PHBV scaffolds and PHBV/BG scaffolds. Short-term in vitro culture followed by long-term in vivo transplantation was performed to evaluate the difference in cartilage regeneration between the cartilage layers grown on PHBV and PHBV/BG scaffolds. The results show that the incorporation of BG into PHBV efficiently improved both the hydrophilicity of the composites and the percentage of adhered cells and promoted cell migration into the inner part the constructs. With prolonged incubation time in vivo, the chondrocyte-scaffold constructs in the PHBV/BG group formed thicker cartilage-like tissue with better biomechanical properties and a higher cartilage matrix content than the constructs in the PHBV/BG group. These results indicate that PHBV/BG scaffolds can be used to prepare better engineered cartilage than pure PHBV. © 2013 Wu et al.


Ling Y.,First Peoples Hospital of Changzhou | Qian X.,First Peoples Hospital of Changzhou | Cao X.,First Peoples Hospital of Changzhou
Wspolczesna Onkologia | Year: 2013

Immune thrombocytopenic purpura (ITP) is an autoimmune disorder, for which rituximab has been proven to be an effective treatment. The response rate was reported to be approximately 60% in refractory ITP patients. However, the response time is slower than expected, and the mechanism of action of rituximab in ITP is still unclear. Thus, sometimes, the use of a combination therapy with rituximab according to different patient conditions is necessary. We report two refractory chronic ITP cases. The two patients were administered a low dose of dexamethasone (10 mg, weekly) combined with rituximab and a smaller dose of prednisone (10 mg, daily) asmaintenance therapy. Although their peripheral B cells were almost eliminated, no complete reaction was observed. Themaintenance therapy with prednisone was helpful in the prevention of bleeding. The patients' responses to rituximab treatment suggest that multiple immunological mechanisms are involved in ITP pathogenesis and that the use of a combination therapy with rituximab according to the different patient conditions is necessary.


Wu L.,First Peoples Hospital of Changzhou | Qiu Y.,Nanjing University | Wang B.,Nanjing University | Zhu Z.Z.,Nanjing University | Ma W.W.,Nanjing University
Spine | Year: 2010

Study Design: A prospective trial of MRI study in patients with "idiopathic" left thoracic scoliosis. Objective: To investigate the prevalence of neural axis abnormalities in asymptomatic patients with "idiopathic" left thoracic scoliosis. Summary of Background Data: Some patients with neural axis abnormalities have scoliosis as the only presenting sign, and they might be given a diagnosis of "idiopathic" scoliosis. These neural axis abnormalities are risk factors for neurologic injury during spine correction. With the development of MRI, neural axis abnormalities are increasingly being found in patients with "idiopathic" scoliosis. However, there are few reports on the prevalence of neural axis abnormalities in asymptomatic patients with "idiopathic" left thoracic scoliosis. Methods: A total of 68 patients with presumed "idiopathic" left thoracic scoliosis were examined for neural axis abnormalities, using MRI. Results: Neural axis abnormalities were detected in 37 (54%) patients, including Chiari 1 malformation in 15 patients, Chiari 1 malformation with syringomyelia in 10, Chiari 1 malformation with syringomyelia and tethered cord in 1, Chiari 1 malformation with syringomyelia and diastematomyelia in 1, syringomyelia in 8, syringomyelia with tethered cord in 1, and arachnoidal cyst in cerebel-lomedullary cistern in 1 patient. There were statistically significant differences between patients with and without neural axis abnormalities regarding gender and curve severity (P < 0.05). Conclusion: When a left thoracic curve pattern is present in patients with "idiopathic" scoliosis, especially in male patients or patients with severe curve, strong consideration should be given to the possibility of the presence of neural axis abnormalities, and acquisition of an MRI scan considered. © 2010, Lippincott Williams & Wilkins.


Shao D.,First Peoples Hospital Of Changzhou | Chen J.-P.,First Peoples Hospital Of Changzhou
World Chinese Journal of Digestology | Year: 2015

AIM: To evaluate the efficacy and safety of endoscopic duodenal papillary balloon dilatation via fistula in the treatment of patients with common bile duct stones and papillary choledochoduodenal fistula. METHODS: Twenty-eight patients with bile duct stones and papillary choledochoduodenal fistula who received endoscopic retrograde cholangiopancreatography (ERCP) examination from June 2009 to October 2014 at our hospital were divided into either an endoscopic sphincterotomy (EST) group or a balloon dilatation group to receive the two procedures, respectively. Clinical effects were compared for the two groups. RESULTS: Stones were successfully removed in all patients. Bleeding occurred in four patients in the EST group and one in the balloon dilatation group. Perforation did not occur in either group. The operation time was longer in the EST group than in the balloon dilation group (P < 0.01). With regard to intraoperative complications, the balloon dilation group was better than the EST group (P < 0.05). Mild abdominal pain occurred in four patients in the EST group and one in the balloon dilation group. Fever occurred in one patient in the EST group and did not occur in the balloon dilation group. Delayed perforation, hemorrhage, severe acute pancreatitis, and suppurative cholangitis did not occur in either group. Postoperative complications in the two groups were different significantly (P < 0.05). CONCLUSION: Endos copi c duodena l papillary balloon dilatation via fistula for treatment of patients with common bile duct stones and papillary choledochoduodenal fistula is safe and effective. © 2015 Baishideng Publishing Group Inc.


Xu Y.,Nantong University | An Y.,First Peoples Hospital of Changzhou | Wang X.,Nanjing Medical University | Zha W.,Nantong University | Li X.,Nanjing Medical University
Oncology Reports | Year: 2014

The HH signaling pathway is a 'core' signal transduction pathway in pancreatic cancer that promotes the tumorigenesis of pancreatic cancers via enhancing cell proliferation, increasing invasion and metastasis and protecting against apoptosis. In the present study, we found that HH signaling regulates autophagy in pancreatic cancer cells. Activation of HH signaling inhibits autophagy, while inhibition of the HH pathway induces autophagy. Although the role of autophagy in cell survival and apoptosis may depend on tumor type and the microenvironment, our data clearly demonstrated that GANT61-induced autophagy contributed to reduced viability and increased apoptosis in pancreatic cancer cells both in vivo and in vitro, and these effects were reversed by the autophagy inhibitor, 3-MA. We propose that HH signaling by regulating autophagy plays an important role in determining the cellular response to HH-targeted therapy in pancreatic cancer and further investigation of the interaction between autophagy and HH signaling is particularly important.


Lu X.,Jiangsu University | Lu J.,First Peoples Hospital of Changzhou
Clinical Laboratory | Year: 2015

Background: Colorectal cancer (CRC) is a severe disease with considerable morbidity and mortality. Stable presence of miRNAs in serum enables them to be biomarkers for early diagnosis and surveillance of the progress of CRC. Methods: The blood samples of S3 CRC patients of I-IV stage pre therapy and 50 controlled healthy people were collected and total serum RNA was extracted. The level of miR-423-5p and miR-484 in serum were detected by qPCR. Results: In stage I - II CRC, serum miR-423-5p was significantly elevated compared with the control, whereas miR-484 was attenuated. In stage III - IV, no significance difference in miR-423-5p level was found between CRC patients and the control, and miR-484 level increased dramatically. Conclusions: The study provides the possibility that the detection of miR-484-5p and miR-484 may be a useful method for early diagnosis and surveillance of the progress of CRC.


PubMed | First Peoples Hospital of Changzhou and Nanjing Medical University
Type: Journal Article | Journal: Mycopathologia | Year: 2016

Pulmonary fungal disease is an emerging issue in immunocompetent patients, for whom the characteristics are only partially understood.We conducted a single-center retrospective study of histologically verified pulmonary fungal disease in Eastern China from 2006 to 2014 to understand the demographics, clinical manifestations, therapeutic approaches, and factors associated with prognosis in this population. All cases were diagnosed according to the 2008 European Organization for the Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infection Diseases Mycoses Study Group definition criteria.A total of 112 cases of pulmonary fungal diseases were enrolled (35 proven, 16 probable, 61 possible), and we analyzed the 35 patients with histologically proven pulmonary fungal diseases in this study. The main fungal species identified were Aspergillus (51.4%), Cryptococcus (22.9%), and Mucor (2.4%). Treatment consisted of antifungal therapeutic agents (54.3%), surgery and postsurgical agents (25.7%), or surgery alone (14.3%). The overall crude mortality rate was 14.3%, and the mortality due to pulmonary fungal infections was 2.9%. Significant predictors of mortality by univariate analysis were hypoalbuminemia (P=0.005), cancer (P=0.008), and positive culture (P=0.044). Additionally, hypoalbuminemia was the only risk factor for mortality by multivariate analysis (RR=7.56, 95% CI 1.38-41.46).Pulmonary fungal disease in immunocompetent patients, with Aspergillus as the most common identified species, had a prognosis that was influenced by the level of serum albumin.


Liu J.,First Peoples Hospital of ChangZhou | Tang T.,Suzhou University | Yang H.,Suzhou University
Injury | Year: 2011

Objective: To observe the protective effect of deferoxamine on experimental spinal cord injury (SCI) in rats. Methods: Sprague-Dawley rats were randomly divided into the following four groups. Control group: rats were performed laminectomy only; SCI group: rats were performed laminectomy with SCI; DFO group: rats were injected intraperitoneally a bolus of 100 mg/kg deferoxamine after SCI; vehicle group: rats were injected intraperitoneally 0.9% saline after SCI. The SCI of animal model was made by using a modified Allen's method on T 10. Six rats of each group were sacrificed at 4 h after injured, and the levels of free iron and malondialdehyde (MDA) of involved spinal cord segments were measured by bleomycin assay and the thiobarbituric acid (TBA) separately. The recovery of function was assessed by Modified Tarlov's scale and inclined plane method at 7, 14, 21 d after SCI. The histologic changes of the damaged spinal cord were also examined at 7 d after SCI. Results: Following SCI, the levels of free iron and MDA were increased significantly and the Modified Tarlov's score and inclined plane angles decreased in SCI group and vehicle group. In DFO group, the levels of free iron and MDA were not increased, but the Modified Tarlov's score and inclined plane angles decreased, the histological findings were improved as well. Conclusion: Deferoxamine can reduce the levels of free iron and lipid peroxidation, and improve the hind limb functional status of rats with spinal cord injury. © 2010 Elsevier Ltd.

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