Wang L.,Zibo Vocational Institute |
Sun S.,First Hospital of Zibo City |
Wang Q.,Shandong Wanjie Medical College
Annals of Laboratory Medicine | Year: 2015
Background: Breast cancer is the most common type of cancer in females. Aberrant expression of microRNA-21 (miR-21) has previously been reported in breast cancer tissue. The aim of this study was to investigate expression levels of serum miR-21 in breast cancer patients and evaluate its prognostic value in Chinese females. Methods: Real-time quantitative (RQ)-PCR was used to analyze miR-21 expression in archived serum, tumor tissue, and adjacent normal tissue from 549 participants (326 with breast cancer, 223 without breast cancer). We also analyzed associations between serum miR-21 expression and breast cancer subtypes and patient prognosis. Recurrence and survival were analyzed by using the multivariate Cox proportional hazards model. Results: Expression of miR-21 was significantly higher in breast cancer tissues compared with normal adjacent breast tissues (P < 0.001). The 2-δδa values for serum miR-21 in breast cancer patients versus healthy controls were 9.12±3.43 and 2.96±0.73, respectively. Multivariate Cox proportional hazards model suggested that serum miR-21 expression was an independent poor prognostic factor for both recurrence (hazard ratio [HR]= 2.942; 95% confidence interval [CI]=1.420-8.325; P =0.008) and disease-free survival (HR=2.732; 95% CI=1.038-7.273, P =0.003) in breast cancer. Conclusions: Increased serum miR-21 expression level was correlated with poor prognosis of breast cancer patients, indicating that serum miR-21 may be a novel prognostic marker for recurrence and survival of breast cancer patients before resection. © The Korean Society for Laboratory Medicine.
Xu D.,First Hospital of Zibo City |
Lv X.,First Hospital of Zibo City |
Bi Z.,First Hospital of Zibo City |
Ren G.,First Hospital of Zibo City |
Wang L.,Shandong University
Chinese Journal of Clinical Oncology | Year: 2014
Objective: To investigate the effect of c-maf gene on the MM cells' proliferation and invasion activity. Methods:Lipofectin Reagent was used to transfect c-maf siRNA into multiple myeloma cell of RPMI8226. The mRNA expression level of c-maf was detected by RT-PCR.Cell growth curve was measured by MTT assay. Transwell chamber test was used to measure MM cells' in vitro invasion activity. The cell cycle distribution were assessed by flow cytomentry. The protein expression levels of survivin, MMP-2, MMP-9, ARK5 and cyclin B1 were detected by Western blot. We also detected the activity of Caspase-3/7. Results: The c-maf siRNA was effectively transfected into cells and the mRNA expression of the c-maf gene was inhibited.MTT test and Transwell chamber test showed that the proliferation and in vitro invasion activity of transfected cells were significantly lower than those of other two groups (P<0.05). Cell cycle of c-maf siRNA transfected group cells was arrested in G2/M phase. The expression levels of survivin, MMP-2, MMP-9, ARK5, cyclin B1 and the activity of Caspase-3/7 between c-maf siRNA transfected group and the other two groups were statistically different (P<0.05). Conclusion: c-maf gene by c-maf siRNA can remarkably inhibit proliferation and invasion of multiple myeloma cell lines of RPMI8226. C-maf gene may be used as the target for multiple myeloma gene therapy.
Jiang X.-J.,First Hospital of Zibo City |
Xia Y.,First Hospital of Zibo City
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: In various clinical treatments of soft tissue defects, vacuum sealing drainage technology can effectively control wound infection, shorten the treatment time, and accelerate wound healing. OBJECTIVE: To further understand the features and characteristics of hydrated ethanol polyethylene foam vacuum sealing drainage treatment, and compare the treatment effects against soft tissue defects with the conventional dressing change treatment. METHODS: A total of 140 cases of large soft tissue defects were divided randomly into two groups. Observation group received hydrated ethanol polyethylene foam vacuum sealing drainage treatment, while control group was treated by conventional dressing change. The degree of pain relief during treatment, the family satisfaction, the survival rate of first skin-grafting, the time needed before skin-grafting, the hospitalization time, the number of operations, the readmission rates, the complication rate and other clinical effectiveness indicators were compared between the two groups. RESULTS AND CONCLUSION: In the observation group, visual analog scale scores decreased 5.76±0.68, in the control group visual analog scale score decreased 2.85±0.53, with significant differences between the two groups (P < 0.05), and family satisfaction degree of the observation group was higher (P < 0.05). The survival rate of first skin-grafting of the observation group was higher than the control group (P < 0.05), the time needed before skin-grafting, the hospitalization time, the number of operations, the readmission rates, and the complication rate were lower than the control group (P < 0.05). The results showed that, compared with conventional dressing change treatment, hydrated ethanol polyethylene foam vacuum sealing drainage has advantages of light pain, short treatment time, low rate of complications, and high efficacy.