First Affiliated Hospital Of Xian Jiaotong University

Xi’an, China

First Affiliated Hospital Of Xian Jiaotong University

Xi’an, China
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Maghaiya D.B.,First Affiliated Hospital Of Xian Jiaotong University | Min G.Z.,First Affiliated Hospital Of Xian Jiaotong University | Yang Y.,First Affiliated Hospital Of Xian Jiaotong University
Biomedical Research (India) | Year: 2017

Background: In 1968, Bentall proposed the artificial vessels with ascending aorta and aortic valve replacement and re-implantation of left and right coronary arteries to treat aortic disease with ascending aortic aneurysmal dilatation. Successful Bentall operation can be achieved by greater myocardial protection, no distortion and unobstructed coronary artery bypass grafting, as well as ensured anastomotic stoma. Patients and methods: From June 2007 to November 2013, 421 consecutive patients with a variety of cardiac pathologies underwent beating heart surgery at our institution utilizing the myocardial perfusion strategies described above. Among them, 21 cases underwent beating heart Bentall surgery, the etiology of patients was Marfan syndrome (5 patients; 23.8%), degenerative disease (2 patients; 9.5%), bicuspid aortic valve (2 patients; 9.5%), Takayasu’s arteritis (1 patient; 4.8%), and rheumatic disease in the remaining 11 patients (52.4%). Surgical Technique: Surgical procedures were performed and valve was resected, and sutured with artificial blood vessels with valve in continuous or interrupted sutures manner in aortic rings. Results: None of the patients had perioperative myocardial infarction. Major complications included low output syndrome in 1 patient (4.8%), need for prolonged mechanical ventilation in 2 (9.5%). Postoperative echocardiographic data were available for 17 of 21 patients (81.0%). 17 patients were evaluated; none of the patients had peri valvular leak or prosthetic valve dysfunction. Conclusion: Bentall operation can improve the functional recovery and quality of life in most of the surviving patients. Outcomes obtained using warm blood retrograde coronary sinus perfusion in beating heart surgery seems to compare favorably to those of traditional cardiac arrest surgery. © 2017, Scientific Publishers of India. All rights reserved.


Wang L.-L.,Affiliated Hospital Of Yanan University | Feng Y.-Q.,Affiliated Hospital Of Yanan University | Cheng Y.-H.,First Affiliated Hospital Of Xian Jiaotong University
International Journal of Ophthalmology | Year: 2017

AIM: To investigate the effect of high mobility group protein box-1 (HMGB1) siRNA on proliferation and apoptosis of retinoblastoma (Rb) cells. ● METHODS: The expression of HMGB1 in Rb cells were detected by real-time polymerase chain reaction (RT-PCR) and Western blot. Chemically synthesized HMGB1 siRNA was transfected into Y79 cells. The inhibitory rate was also examined by RT-PCR and Western blot. After HMGB1 siRNA transfection, the cell proliferation was analyzed by MTT, and cell apoptosis was detected by Caspase-3 active detection kit. Cell cycle distribution and apoptosis were detected by flow cytometry. ● RESULTS: The expression of HMGB1 significantly ele-vated in Rb cells (P <0.01). After transfected by siRNA, the HMGB1 protein level of Y79 cells was significantly reduced (P <0.01). After siRNA interference HMGB1, the proportion of proliferating cells reduced, and the proportion of quiescent cells increased (P <0.05). In addition, apoptosis rate of Y79 cells increased from 2.03% to 9.10% after interfering with HMGB1 siRNA (P <0.05). ● CONCLUSION: Specific HMGB1 siRNA can inhibit the expression of HMGB1. The effect may be attributed to inhibit the proliferation and promote cell apoptosis. © 2017, International Journal of Ophthalmology (c/o Editorial Office). All rights reserved.


Li J.,First Affiliated Hospital Of Xian Jiaotong University | Ge Z.,Honghui Hospital Of Xian Jiaotong University | Fan L.,Second Affiliated Hospital Of Xian Jiaotong University | Wang K.,Second Affiliated Hospital Of Xian Jiaotong University
BMC Musculoskeletal Disorders | Year: 2017

Background: The objective of this study was to investigate the protective effects of molecular hydrogen, a novel and selective antioxidant, on steroid-induced osteonecrosis (ON) in a rabbit model. Methods: Sixty rabbits were randomly divided into two groups (model group and hydrogen group). Osteonecrosis was induced according to an established protocol of steroid-induced ON. Rabbits in the hydrogen group were treated with intraperitoneal injections of molecular hydrogen at 10 ml/kg body weight for seven consecutive days. Plasma levels of total cholesterol, triglycerides, soluble thrombomodulin(sTM), glutathione(GSH) and malondialdehyde(MDA) were measured before and after steroid administration. The presence or absence of ON was examined histopathologically. Oxidative injury and vascular injury were assessed in vivo by immunohistochemical staining of 8-hydoxy-2-deoxyguanosine(8-OHdG) and MDA, and ink artery infusion angiography. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assays were performed to measure apoptosis. Results: The incidence of steroid-induced ON was significantly lower in hydrogen group (28.6%) than that in model group (68.0%). No statistically differences were observed on the levels of total cholesterol and triglycerides. Oxidative injury, vascular injury and apoptosis were attenuated in the hydrogen group compared with those in the model group in vivo. Conclusions: These results suggested that molecular hydrogen prevents steroid-induced osteonecrosis in rabbits by suppressing oxidative injury, vascular injury and apoptosis. © 2017 The Author(s).


Yan F.,Third Affiliated Hospital Of Xian Jiaotong University | Yue W.,Tianjin Huanhu Hospital | Zhang Y.-L.,Third Affiliated Hospital Of Xian Jiaotong University | Mao G.-C.,Third Affiliated Hospital Of Xian Jiaotong University | And 4 more authors.
Neural Regeneration Research | Year: 2015

In this study, we successfully constructed a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold in vitro, transplanted either the composite or bone marrow mesenchymal stem cells alone into the ischemic area in animal models, and compared their effects. At 14 days after co-transplantation of bone marrow mesenchymal stem cells and the hitosan- collagen scaffold, neurological function recovered noticeably. Vascular endothelial growth factor expression and nestin-labeled neural precursor cells were detected in the ischemic area, surrounding tissue, hippocampal dentate gyrus and subventricular zone. Simultaneously, a high level of expression of glial fibrillary acidic protein and a low level of expression of neuron-specific enolase were visible in BrdU-labeled bone marrow mesenchymal stem cells. These findings suggest that transplantation of a composite of bone marrow mesenchymal stem cells and a chitosan- collagen scaffold has a neuroprotective effect following ischemic stroke. © 2015, Editorial Board of Neural Regeneration Research. All rights reserved.


Gao Y.,First Affiliated Hospital Of Xian Jiaotong University | Gao F.,First Affiliated Hospital Of Xian Medical University | Chen K.,Zhejiang Sci-Tech University | Tian M.-L.,First Affiliated Hospital Of Xian Jiaotong University | Zhao D.-L.,First Affiliated Hospital Of Xian Jiaotong University
Drug Design, Development and Therapy | Year: 2015

The development of chemotherapeutic resistance is a major challenge in oncology. Elevated sphingosine kinase 1 (SK1) levels is predictive of a poor prognosis, and SK1 overexpression may confer resistance to chemotherapeutics. The SK/sphingosine-1-phosphate (S1P)/sphingosine-1-phosphate receptor (S1PR) signaling pathway has been implicated in the progression of various cancers and in chemotherapeutic drug resistance. Therefore, SK1 may represent an important target for cancer therapy. Targeting the SK/S1P/S1PR signaling pathway may be an effective anticancer therapeutic strategy, particularly in the context of overcoming drug resistance. This review summarizes our current understanding of the role of SK/S1P/S1PR signaling in cancer and development of SK1 inhibitors. © 2015 Gao et al.


Li P.,First Affiliated Hospital Of Xian Jiaotong University | Mao X.,First Affiliated Hospital Of Xian Jiaotong University | Ren Y.,First Affiliated Hospital Of Xian Jiaotong University | Liu P.,First Affiliated Hospital Of Xian Jiaotong University
International Journal of Biological Sciences | Year: 2014

Cell polarity, which is defined as asymmetry in cell shape, organelle distribution and cell function, is essential in numerous biological processes, including cell growth, cell migration and invasion, molecular transport, and cell fate. Epithelial cell polarity is mainly regulated by three conserved polarity protein complexes, the Crumbs (CRB) complex, partitioning defective (PAR) complex and Scribble (SCRIB) complex. Research evidence has indicated that dysregulation of cell polarity proteins may play an important role in cancer development. Crumbs homolog 3 (CRB3), a member of the CRB complex, may act as a cancer suppressor in mouse kidney epithelium and mouse mammary epithelium. In this review, we focus on the current data available on the roles of CRB3 in cancer development. © 2014, Ivyspring International Publisher.


Yan X.-T.,First Affiliated Hospital Of Xian Jiaotong University
European Journal of Clinical Nutrition | Year: 2016

Background/Objectives:Effects of vitamin D deficiency in pregnancy have been associated with some adverse pregnancy outcomes. The objective of this study was to analyze the relationship between vitamin D deficiency in childbearing aged women and pregnancy loss (PL) in the first trimester.Subjects/Methods:This is a cross-sectional study. Plasma was collected from 60 nulliparous women with singleton at 7–9 weeks of gestation (30 with viable gestation and 30 with PL) and 60 non-gravid childbearing aged women (30 with a successful pregnancy history, and 30 with one or more spontaneous first-trimester PL history). Quantitation of serum 25-hydroxyvitamin D (25(OH)D) and 25-hydroxyvitamin D-1 alpha hydroxylase (CYP27B1) was assayed.Results:By pregnancy/non-gravid, normal pregnant women had higher 25(OH)D (49.32 μg/l) and CYP27B1 (82.00 pg/ml) than PL women (34.49 μg/l and 37.87 pg/ml, both P<0.01); the non-gravid women with a successful pregnancy history also had higher 25(OH)D (39.56 μg/l) and CYP27B1 (39.04 pg/ml) than women with PL history (12.30 μg/l and 12.35 pg/ml, both P<0.01). The 96.7% of non-gravid women with PL history and 43.3% of PL women had serum 25(OH)D concentrations below 30 μg/l. There was a strong association between low vitamin D levels and PL (odds ratio 1.71; 95% confidence interval: 1.2–2.4, P<0.001). The regression analyses showed that PL was significantly inversely correlated with 25(OH)D (P<0.01) and CYP27B1 levels (P<0.01).Conclusions:Vitamin D deficiency associated with PL in the first trimester of pregnancy. Decreased serum vitamin D levels among childbearing aged women with the failed clinical pregnancies history may predispose to increased risk for PL.European Journal of Clinical Nutrition advance online publication, 25 May 2016; doi:10.1038/ejcn.2016.83. © 2016 Macmillan Publishers Limited


Wang X.,First Affiliated Hospital Of Xian Jiaotong University | Hu Y.,First Affiliated Hospital Of Xian Jiaotong University | Ren M.,First Affiliated Hospital Of Xian Jiaotong University | Lu X.,First Affiliated Hospital Of Xian Jiaotong University | And 2 more authors.
Korean Journal of Radiology | Year: 2016

Objective: To compare the efficacy and safety of combined radiofrequency ablation (RFA) and transcatheter arterial chemoembolization (TACE) with RFA alone for hepatocellular carcinomas (HCC). Materials and Methods: Randomized controlled trial (RCT) studies that compared the clinical or oncologic outcomes of combination therapy of TACE and RFA versus RFA for the treatment of HCC were identified through literature searches of electronic databases (Pubmed, Embase, Cochrane Library, China Biology Medicine disc, China National Knowledge Infrastructure, and Google Scholar). Hazard ratios (HRs) or odds ratios (ORs) with their corresponding 95% confidence interval (CI) were combined as the effective value to assess the summary effects. The strength of evidence was rated by the Grading of Recommendations Assessment, Development, and Evaluation system. Results: Six RCTs with 534 patients were eligible for inclusion in this meta-analysis. The meta-analysis showed that the combination of TACE and RFA is associated with a significantly longer overall survival (HR = 0.62, 95% CI: 0.49–0.78, p < 0.001) and recurrence-free survival (HR = 0.55, 95% CI: 0.40–0.76, p < 0.001) in contrast with RFA monotherapy. The seemingly higher incidence of major complications in the combination group compared with RFA group did not reach statistical significance (OR = 1.17, 95% CI: 0.39–3.55, p = 0.78). Conclusion: In patients with HCC, the combination of TACE and RFA is associated with significantly higher overall survival and recurrence-free survival, as compared with RFA monotherapy, without significant difference in major complications. © 2016 The Korean Society of Radiology.


Liu S.,First Affiliated Hospital Of Xian Jiaotong University | Yi Z.,First Affiliated Hospital Of Xian Jiaotong University | Ling M.,First Affiliated Hospital Of Xian Jiaotong University | Shi J.,First Affiliated Hospital Of Xian Jiaotong University | And 2 more authors.
Tumor Biology | Year: 2014

Genetic polymorphisms in drug metabolism and transport genes can influence the pharmacokinetics and pharmacodynamics of chemotherapy drugs. We investigated the role of genes involved in metabolic and transport pathways in response to chemotherapy and clinical outcome of osteosarcoma patients. The association between the eight polymorphisms with response to chemotherapy and clinical outcome of patients was carried out by unconditional logistic regression analysis and Cox proportional hazard models. Of 186 patients, 98 patients showed good response to chemotherapy, 64 died, and 97 showed progression at the end of the study. Patients carrying ABCB1 rs1128503 TT genotype and T allele were more likely to have a good response to chemotherapy. ABCC3 rs4148416 TT genotype and T allele and GSTP1 rs1695 GG genotype and G allele were associated with poor response to chemotherapy. In the Cox proportional hazards model, after adjusting for potential confounding factors, patients carrying ABCB1 rs1128503 TT genotype and T allele were associated with lower risk of progression-free survival (PFS) and overall survival (OS). ABCC3 rs4148416 TT genotype and T allele and GSTP1 rs1695 GG genotype and G allele were correlated with high risk of PFS and OS. The ABCB1 TT and GSTP1 GG genotypes were significantly associated with a shorter OS. In conclusion, variants of ABCB1 rs128503, ABCC3 rs4148416, and GSTP1 rs1695 are associated with response to chemotherapy and PFS and OS of osteosarcoma patients; these gene polymorphisms could help in the design of individualized therapy. © 2014, International Society of Oncology and BioMarkers (ISOBM).


Li Y.,First Affiliated Hospital Of Xian Jiaotong University | Mi C.,First Affiliated Hospital Of Xian Jiaotong University | Li W.,First Affiliated Hospital Of Xian Jiaotong University | She J.,First Affiliated Hospital Of Xian Jiaotong University
Digestive Diseases and Sciences | Year: 2016

Background: Acute appendicitis is the most common abdominal emergency, but the diagnosis of appendicitis remains a challenge. Endoscopic retrograde appendicitis therapy (ERAT) is a new and minimally invasive procedure for the diagnosis and treatment of acute appendicitis. Aim: To investigate the diagnostic value of ERAT for acute appendicitis by the combination of colonoscopy and endoscopic retrograde appendicography (ERA). Methods: Twenty-one patients with the diagnosis of suspected uncomplicated acute appendicitis who underwent ERAT between November 2014 and January 2015 were included in this study. The main outcomes, imaging findings of acute appendicitis including colonoscopic direct-vision imaging and fluoroscopic ERA imaging, were retrospectively reviewed. Secondary outcomes included mean operative time, mean hospital stay, rate of complication, rate of appendectomy during follow-up period, and other clinical data. Results: The diagnosis of acute appendicitis was established in 20 patients by positive ERA (5 patients) or colonoscopy (1 patient) alone or both (14 patients). The main colonoscopic imaging findings included mucosal inflammation (15/20, 75 %), appendicoliths (14/20, 70 %), and maturation (5/20, 25 %). The key points of ERA for diagnosing acute appendicitis included radiographic changes of appendix (17/20, 85 %), intraluminal appendicoliths (14/20, 70 %), and perforation (1/20, 5 %). Mean operative time of ERAT was 49.7 min, and mean hospital stay was 3.3 days. No patient converted to emergency appendectomy. Perforation occurred in one patient after appendicoliths removal was not severe and did not require invasive procedures. During at least 1-year follow-up period, only one patient underwent laparoscopic appendectomy. Conclusion: ERAT is a valuable procedure of choice providing a precise yield of diagnostic information for patients with suspected acute appendicitis by combination of colonoscopy and ERA. © 2016 Springer Science+Business Media New York

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